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Title: In situ architecture of the algal nuclear pore complex

Abstract

Nuclear pore complexes (NPCs) span the nuclear envelope and mediate nucleocytoplasmic exchange. They are a hallmark of eukaryotes and deeply rooted in the evolutionary origin of cellular compartmentalization. NPCs have an elaborate architecture that has been well studied in vertebrates. Whether this architecture is unique or varies significantly in other eukaryotic kingdoms remains unknown, predominantly due to missing in situ structural data. Here, we report the architecture of the algal NPC from the early branching eukaryote Chlamydomonas reinhardtii and compare it to the human NPC. We find that the inner ring of the Chlamydomonas NPC has an unexpectedly large diameter, and the outer rings exhibit an asymmetric oligomeric state that has not been observed or predicted previously. Our study provides evidence that the NPC is subject to substantial structural variation between species. The divergent and conserved features of NPC architecture provide insights into the evolution of the nucleocytoplasmic transport machinery.

Authors:
ORCiD logo [1];  [2];  [3];  [3];  [4]; ORCiD logo [4]; ORCiD logo [4];  [3];  [3]; ORCiD logo [3]; ORCiD logo [1]
  1. European Molecular Biology Lab., Heidelberg (Germany)
  2. European Molecular Biology Lab., Heidelberg (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  3. Max Planck Inst. for Biochemistry, Martinsried (Germany)
  4. Univ. of California, Los Angeles, CA (United States)
Publication Date:
Research Org.:
Univ. of California, Los Angeles, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1511713
Grant/Contract Number:  
FC02-02ER63421
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mosalaganti, Shyamal, Kosinski, Jan, Albert, Sahradha, Schaffer, Miroslava, Strenkert, Daniela, Salomé, Patrice A., Merchant, Sabeeha S., Plitzko, Jürgen M., Baumeister, Wolfgang, Engel, Benjamin D., and Beck, Martin. In situ architecture of the algal nuclear pore complex. United States: N. p., 2018. Web. doi:10.1038/s41467-018-04739-y.
Mosalaganti, Shyamal, Kosinski, Jan, Albert, Sahradha, Schaffer, Miroslava, Strenkert, Daniela, Salomé, Patrice A., Merchant, Sabeeha S., Plitzko, Jürgen M., Baumeister, Wolfgang, Engel, Benjamin D., & Beck, Martin. In situ architecture of the algal nuclear pore complex. United States. doi:10.1038/s41467-018-04739-y.
Mosalaganti, Shyamal, Kosinski, Jan, Albert, Sahradha, Schaffer, Miroslava, Strenkert, Daniela, Salomé, Patrice A., Merchant, Sabeeha S., Plitzko, Jürgen M., Baumeister, Wolfgang, Engel, Benjamin D., and Beck, Martin. Mon . "In situ architecture of the algal nuclear pore complex". United States. doi:10.1038/s41467-018-04739-y. https://www.osti.gov/servlets/purl/1511713.
@article{osti_1511713,
title = {In situ architecture of the algal nuclear pore complex},
author = {Mosalaganti, Shyamal and Kosinski, Jan and Albert, Sahradha and Schaffer, Miroslava and Strenkert, Daniela and Salomé, Patrice A. and Merchant, Sabeeha S. and Plitzko, Jürgen M. and Baumeister, Wolfgang and Engel, Benjamin D. and Beck, Martin},
abstractNote = {Nuclear pore complexes (NPCs) span the nuclear envelope and mediate nucleocytoplasmic exchange. They are a hallmark of eukaryotes and deeply rooted in the evolutionary origin of cellular compartmentalization. NPCs have an elaborate architecture that has been well studied in vertebrates. Whether this architecture is unique or varies significantly in other eukaryotic kingdoms remains unknown, predominantly due to missing in situ structural data. Here, we report the architecture of the algal NPC from the early branching eukaryote Chlamydomonas reinhardtii and compare it to the human NPC. We find that the inner ring of the Chlamydomonas NPC has an unexpectedly large diameter, and the outer rings exhibit an asymmetric oligomeric state that has not been observed or predicted previously. Our study provides evidence that the NPC is subject to substantial structural variation between species. The divergent and conserved features of NPC architecture provide insights into the evolution of the nucleocytoplasmic transport machinery.},
doi = {10.1038/s41467-018-04739-y},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {2018},
month = {6}
}

Journal Article:
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Cited by: 16 works
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Figures / Tables:

Fig. 1 Fig. 1: Structure of the CrNPC in comparison to the HsNPC. a Structures are displayed as rendered isosurfaces, sliced through the central axis. Green arrowheads indicate the connector element, which is absent from the cytoplasmic side of the CrNPC. b Cytoplasmic face view. The CrNPC central channel is dilated. cmore » Cytoplasmic and nuclear rings of both NPCs. The CrNPC cytoplasmic ring has reduced density compared to the HsNPC. Black and magenta arrowheads indicate the density attributed to the Nup159 (Nup214 in humans) subcomplex, which forms cytoplasmic filaments that protrude towards the central channel. CR cytoplasmic ring, IR inner ring, and NR nuclear ring« less

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