Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Molecular architecture of fungal cell walls revealed by solid-state NMR

Abstract

The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets. The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive. Here we report the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus. Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan. The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence. This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1];  [1];  [2]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [1]
+ Show Author Affiliations
  1. Louisiana State Univ., Baton Rouge, LA (United States)
  2. Univ. of Georgia, Athens, GA (United States)
  3. Louisiana State Univ. Health Sciences Center, New Orleans, LA (United States)
  4. Florida State Univ., Tallahassee, FL (United States). National High Magnetic Field Lab. (MagLab)
Publication Date:
Research Org.:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division
OSTI Identifier:
1511439
Grant/Contract Number:  
SC0015662
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Kang, Xue, Kirui, Alex, Muszyński, Artur, Widanage, Malitha C. Dickwella, Chen, Adrian, Azadi, Parastoo, Wang, Ping, Mentink-Vigier, Frederic, and Wang, Tuo. Molecular architecture of fungal cell walls revealed by solid-state NMR. United States: N. p., 2018. Web. doi:10.1038/s41467-018-05199-0.
Copy to clipboard
Kang, Xue, Kirui, Alex, Muszyński, Artur, Widanage, Malitha C. Dickwella, Chen, Adrian, Azadi, Parastoo, Wang, Ping, Mentink-Vigier, Frederic, & Wang, Tuo. Molecular architecture of fungal cell walls revealed by solid-state NMR. United States. https://doi.org/10.1038/s41467-018-05199-0
Copy to clipboard
Kang, Xue, Kirui, Alex, Muszyński, Artur, Widanage, Malitha C. Dickwella, Chen, Adrian, Azadi, Parastoo, Wang, Ping, Mentink-Vigier, Frederic, and Wang, Tuo. Mon . "Molecular architecture of fungal cell walls revealed by solid-state NMR". United States. https://doi.org/10.1038/s41467-018-05199-0. https://www.osti.gov/servlets/purl/1511439.
Copy to clipboard
@article{osti_1511439,
title = {Molecular architecture of fungal cell walls revealed by solid-state NMR},
author = {Kang, Xue and Kirui, Alex and Muszyński, Artur and Widanage, Malitha C. Dickwella and Chen, Adrian and Azadi, Parastoo and Wang, Ping and Mentink-Vigier, Frederic and Wang, Tuo},
abstractNote = {The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets. The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive. Here we report the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus. Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan. The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence. This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.},
doi = {10.1038/s41467-018-05199-0},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {2018},
month = {7}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1038/s41467-018-05199-0
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 141 works
Citation information provided by
Web of Science
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Invasive Aspergillosis
journal, November 1983

The Target of β-Expansin EXPB1 in Maize Cell Walls from Binding and Solid-State NMR Studies
journal, October 2016

  • Wang, Tuo; Chen, Yuning; Tabuchi, Akira
  • Plant Physiology, Vol. 172, Issue 4
  • DOI: 10.1104/pp.16.01311

A simple method for quantitative determination of polysaccharides in fungal cell walls
journal, December 2006

Secondary Structure, Dynamics, and Topology of a Seven-Helix Receptor in Native Membranes, Studied by Solid-State NMR Spectroscopy
journal, January 2007

  • Etzkorn, Manuel; Martell, Swetlana; Andronesi, Ovidiu C.
  • Angewandte Chemie International Edition, Vol. 46, Issue 3
  • DOI: 10.1002/anie.200602139

Cell wall analysis
journal, July 2004

Surface hydrophobin prevents immune recognition of airborne fungal spores
journal, August 2009

  • Aimanianda, Vishukumar; Bayry, Jagadeesh; Bozza, Silvia
  • Nature, Vol. 460, Issue 7259
  • DOI: 10.1038/nature08264

Structural Rearrangements of Membrane Proteins Probed by Water-Edited Solid-State NMR Spectroscopy
journal, January 2009

  • Ader, Christian; Schneider, Robert; Seidel, Karsten
  • Journal of the American Chemical Society, Vol. 131, Issue 1
  • DOI: 10.1021/ja806306e

Highly Efficient, Water-Soluble Polarizing Agents for Dynamic Nuclear Polarization at High Frequency
journal, August 2013

  • Sauvée, Claire; Rosay, Melanie; Casano, Gilles
  • Angewandte Chemie International Edition, Vol. 52, Issue 41
  • DOI: 10.1002/anie.201304657

Highly Efficient, Water-Soluble Polarizing Agents for Dynamic Nuclear Polarization at High Frequency
journal, August 2013

  • Sauvée, Claire; Rosay, Melanie; Casano, Gilles
  • Angewandte Chemie, Vol. 125, Issue 41
  • DOI: 10.1002/ange.201304657

Quality Control Parameters for Broth Microdilution Tests of Anidulafungin
journal, January 2004

  • Krisher, Karen; Brown, Steven D.; Traczewski, Maria M.
  • Journal of Clinical Microbiology, Vol. 42, Issue 1
  • DOI: 10.1128/jcm.42.1.490.2004

High Frequency Dynamic Nuclear Polarization
journal, April 2013

  • Ni, Qing Zhe; Daviso, Eugenio; Can, Thach V.
  • Accounts of Chemical Research, Vol. 46, Issue 9
  • DOI: 10.1021/ar300348n

Revisit of α-Chitin Crystal Structure Using High Resolution X-ray Diffraction Data
journal, May 2009

  • Sikorski, Pawel; Hori, Ritsuko; Wada, Masahisa
  • Biomacromolecules, Vol. 10, Issue 5
  • DOI: 10.1021/bm801251e

Aspergillosis Case-Fatality Rate: Systematic Review of the Literature
journal, February 2001

  • Lin, S. -J.; Schranz, J.; Teutsch, S. M.
  • Clinical Infectious Diseases, Vol. 32, Issue 3
  • DOI: 10.1086/318483

Current perspectives on echinocandin class drugs
journal, April 2011

Cross polarization in the tilted frame: assignment and spectral simplification in heteronuclear spin systems
journal, December 1998

Analysis of peptidogalactomannans from the mycelial surface of Aspergillus fumigatus
journal, January 1998

Multidimensional solid-state NMR spectroscopy of plant cell walls
journal, September 2016

Phosphoethanolamine cellulose: A naturally produced chemically modified cellulose
journal, January 2018

  • Thongsomboon, Wiriya; Serra, Diego O.; Possling, Alexandra
  • Science, Vol. 359, Issue 6373
  • DOI: 10.1126/science.aao4096

Histoplasma capsulatum  -(1,3)-glucan blocks innate immune recognition by the beta-glucan receptor
journal, January 2007

  • Rappleye, C. A.; Eissenberg, L. G.; Goldman, W. E.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 4
  • DOI: 10.1073/pnas.0609848104

Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls
journal, September 2013

  • Wang, T.; Park, Y. B.; Caporini, M. A.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 41
  • DOI: 10.1073/pnas.1316290110

The cell wall: a carbohydrate armour for the fungal cell
journal, October 2007

Water–Polysaccharide Interactions in the Primary Cell Wall of Arabidopsis thaliana from Polarization Transfer Solid-State NMR
journal, July 2014

  • White, Paul B.; Wang, Tuo; Park, Yong Bum
  • Journal of the American Chemical Society, Vol. 136, Issue 29
  • DOI: 10.1021/ja504108h

Rapid Natural-Abundance 2D 13 C- 13 C Correlation Spectroscopy Using Dynamic Nuclear Polarization Enhanced Solid-State NMR and Matrix-Free Sample Preparation
journal, October 2012

  • Takahashi, Hiroki; Lee, Daniel; Dubois, Lionel
  • Angewandte Chemie International Edition, Vol. 51, Issue 47
  • DOI: 10.1002/anie.201206102

Relaxation-compensated difference spin diffusion NMR for detecting 13C–13C long-range correlations in proteins and polysaccharides
journal, December 2014

  • Wang, Tuo; Williams, Jonathan K.; Schmidt-Rohr, Klaus
  • Journal of Biomolecular NMR, Vol. 61, Issue 2
  • DOI: 10.1007/s10858-014-9889-0

Fungal glycans and the innate immune recognition
journal, October 2014

  • Barreto-Bergter, Eliana; Figueiredo, Rodrigo T.
  • Frontiers in Cellular and Infection Microbiology, Vol. 4
  • DOI: 10.3389/fcimb.2014.00145

Matrix-free dynamic nuclear polarization enables solid-state NMR 13C–13C correlation spectroscopy of proteins at natural isotopic abundance
journal, January 2013

  • Takahashi, Hiroki; Hediger, Sabine; De Paëpe, Gaël
  • Chemical Communications, Vol. 49, Issue 82
  • DOI: 10.1039/c3cc45195j

Research data supporting “Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid-state NMR”
dataset, January 2016

  • Simmons, Thomas J.; Mortimer, Jenny C.; Bernardinelli, Oigres D.
  • Apollo - University of Cambridge Repository
  • DOI: 10.17863/cam.5896

From the surface to the inner layer of the fungal cell wall
journal, February 1997

  • Fontaine, T.; Mouyna, I.; Hartland, R. P.
  • Biochemical Society Transactions, Vol. 25, Issue 1
  • DOI: 10.1042/bst0250194

Proton assisted recoupling and protein structure determination
journal, December 2008

  • De Paëpe, Gaël; Lewandowski, Józef R.; Loquet, Antoine
  • The Journal of Chemical Physics, Vol. 129, Issue 24
  • DOI: 10.1063/1.3036928

Determination of Through-Bond Carbon−Carbon Connectivities in Solid-State NMR Using the INADEQUATE Experiment
journal, August 1997

  • Lesage, Anne; Auger, Céline; Caldarelli, Stefano
  • Journal of the American Chemical Society, Vol. 119, Issue 33
  • DOI: 10.1021/ja971089k

Dynamic Nuclear Polarization Surface Enhanced NMR Spectroscopy
journal, March 2013

  • Rossini, Aaron J.; Zagdoun, Alexandre; Lelli, Moreno
  • Accounts of Chemical Research, Vol. 46, Issue 9
  • DOI: 10.1021/ar300322x

Homonuclear radio frequency-driven recoupling in rotating solids
journal, June 1998

  • Bennett, Andrew E.; Rienstra, Chad M.; Griffiths, Janet M.
  • The Journal of Chemical Physics, Vol. 108, Issue 22
  • DOI: 10.1063/1.476420

Theoretical aspects of Magic Angle Spinning - Dynamic Nuclear Polarization
journal, September 2015

  • Mentink-Vigier, Frederic; Akbey, Ümit; Oschkinat, Hartmut
  • Journal of Magnetic Resonance, Vol. 258
  • DOI: 10.1016/j.jmr.2015.07.001

Determination of Through-Bond Carbon−Carbon Connectivities in Solid-State NMR Using the INADEQUATE Experiment
journal, August 1997

  • Lesage, Anne; Auger, Céline; Caldarelli, Stefano
  • Journal of the American Chemical Society, Vol. 119, Issue 33
  • DOI: 10.1021/ja971089k

Fungal glycans and the innate immune recognition
journal, October 2014

  • Barreto-Bergter, Eliana; Figueiredo, Rodrigo T.
  • Frontiers in Cellular and Infection Microbiology, Vol. 4
  • DOI: 10.3389/fcimb.2014.00145

Rotational-echo double-resonance NMR
journal, January 1989

The structure and synthesis of the fungal cell wall
journal, January 2006

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field
journal, October 2014

  • Koers, Eline J.; van der Cruijsen, Elwin A. W.; Rosay, Melanie
  • Journal of Biomolecular NMR, Vol. 60, Issue 2-3
  • DOI: 10.1007/s10858-014-9865-8

Invasive Aspergillosis
journal, November 1988

The structure and synthesis of the fungal cell wall
journal, January 2006

Architecture of the Yeast Cell Wall: THE LINKAGE BETWEEN CHITIN AND β(13)-GLUCAN
journal, January 1995

  • Kollár, Roman; Petráková, Eva; Ashwell, Gilbert
  • Journal of Biological Chemistry, Vol. 270, Issue 3
  • DOI: 10.1074/jbc.270.3.1170

15 N− 15 N Proton Assisted Recoupling in Magic Angle Spinning NMR
journal, April 2009

  • Lewandowski, Józef R.; Paëpe, Gaël De; Eddy, Matthew T.
  • Journal of the American Chemical Society, Vol. 131, Issue 16
  • DOI: 10.1021/ja806578y

Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid-state NMR
text, January 2016

  • Simmons, Thomas; Mortimer, Jennifer; Bernardinelli, Od
  • Apollo - University of Cambridge Repository
  • DOI: 10.17863/cam.7288

Multidimensional solid-state NMR spectroscopy of plant cell walls
journal, September 2016

Homonuclear radio frequency-driven recoupling in rotating solids
journal, June 1998

  • Bennett, Andrew E.; Rienstra, Chad M.; Griffiths, Janet M.
  • The Journal of Chemical Physics, Vol. 108, Issue 22
  • DOI: 10.1063/1.476420

An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls
journal, October 2017

  • Grantham, Nicholas J.; Wurman-Rodrich, Joel; Terrett, Oliver M.
  • Nature Plants, Vol. 3, Issue 11
  • DOI: 10.1038/s41477-017-0030-8

Chitosan: An Update on Potential Biomedical and Pharmaceutical Applications
journal, August 2015

  • Cheung, Randy; Ng, Tzi; Wong, Jack
  • Marine Drugs, Vol. 13, Issue 8
  • DOI: 10.3390/md13085156

A simple and rapid method for the permethylation of carbohydrates
journal, August 1984

Invasive Aspergillosis
journal, April 1998

  • Denning, David W.
  • Clinical Infectious Diseases, Vol. 26, Issue 4
  • DOI: 10.1086/513943

De novo determination of peptide structure with solid-state magic-angle spinning NMR spectroscopy
journal, July 2002

  • Rienstra, C. M.; Tucker-Kellogg, L.; Jaroniec, C. P.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 16
  • DOI: 10.1073/pnas.152346599

Secular Trends in the Epidemiology of Nosocomial Fungal Infections in the United States, 1980-1990
journal, May 1993

The cell wall: a carbohydrate armour for the fungal cell
journal, October 2007

Broadband homonuclear correlation spectroscopy driven by combined <mml:math altimg="si1.gif" overflow="scroll" xmlns:xocs="http://www.elsevier.com/xml/xocs/dtd" xmlns:xs="http://www.w3.org/2001/XMLSchema" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://www.elsevier.com/xml/ja/dtd" xmlns:ja="http://www.elsevier.com/xml/ja/dtd" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:tb="http://www.elsevier.com/xml/common/table/dtd" xmlns:sb="http://www.elsevier.com/xml/common/struct-bib/dtd" xmlns:ce="http://www.elsevier.com/xml/common/dtd" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:cals="http://www.elsevier.com/xml/common/cals/dtd" xmlns:sa="http://www.elsevier.com/xml/common/struct-aff/dtd"><mml:mrow><mml:mtext>R</mml:mtext><mml:msubsup><mml:mrow><mml:mn>2</mml:mn></mml:mrow><mml:mrow><mml:mi>n</mml:mi></mml:mrow><mml:mrow><mml:mi>v</mml:mi></mml:mrow></mml:msubsup></mml:mrow></mml:math> sequences under fast magic angle spinning for NMR structural analysis of organic and biological solids
journal, July 2013

β-glucan: Crucial component of the fungal cell wall and elusive MAMP in plants
journal, May 2016

Revisit of α-Chitin Crystal Structure Using High Resolution X-ray Diffraction Data
journal, May 2009

  • Sikorski, Pawel; Hori, Ritsuko; Wada, Masahisa
  • Biomacromolecules, Vol. 10, Issue 5
  • DOI: 10.1021/bm801251e

Aspergillus fumigatus and Aspergillosis
journal, April 1999

Antifungal Agents: Mode of Action, Mechanisms of Resistance, and Correlation of These Mechanisms with Bacterial Resistance
journal, October 1999

  • Ghannoum, Mahmoud A.; Rice, Louis B.
  • Clinical Microbiology Reviews, Vol. 12, Issue 4
  • DOI: 10.1128/CMR.12.4.501

An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls
text, January 2017

  • Grantham, Nj; Wurman-Rodrich, J.; Terrett, Oliver
  • Apollo - University of Cambridge Repository
  • DOI: 10.17863/cam.13865

Solid-state NMR Reveals the Carbon-based Molecular Architecture of Cryptococcus neoformans Fungal Eumelanins in the Cell Wall
journal, March 2015

  • Chatterjee, Subhasish; Prados-Rosales, Rafael; Itin, Boris
  • Journal of Biological Chemistry, Vol. 290, Issue 22
  • DOI: 10.1074/jbc.m114.618389

Cell Wall Assembly in Saccharomyces cerevisiae
journal, June 2006

  • Lesage, G.; Bussey, H.
  • Microbiology and Molecular Biology Reviews, Vol. 70, Issue 2
  • DOI: 10.1128/MMBR.00038-05

Melanin is an essential component for the integrity of the cell wall of Aspergillus fumigatus conidia
journal, January 2009

From the surface to the inner layer of the fungal cell wall
journal, February 1997

  • Fontaine, T.; Mouyna, I.; Hartland, R. P.
  • Biochemical Society Transactions, Vol. 25, Issue 1
  • DOI: 10.1042/bst0250194

Cell wall analysis
journal, July 2004

Secondary Structure, Dynamics, and Topology of a Seven-Helix Receptor in Native Membranes, Studied by Solid-State NMR Spectroscopy
journal, January 2007

  • Etzkorn, Manuel; Martell, Swetlana; Andronesi, Ovidiu C.
  • Angewandte Chemie International Edition, Vol. 46, Issue 3
  • DOI: 10.1002/anie.200602139

Water–Polysaccharide Interactions in the Primary Cell Wall of Arabidopsis thaliana from Polarization Transfer Solid-State NMR
journal, July 2014

  • White, Paul B.; Wang, Tuo; Park, Yong Bum
  • Journal of the American Chemical Society, Vol. 136, Issue 29
  • DOI: 10.1021/ja504108h

Comparative structure and biomechanics of plant primary and secondary cell walls
journal, January 2012

Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls
journal, September 2013

  • Wang, T.; Park, Y. B.; Caporini, M. A.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 41
  • DOI: 10.1073/pnas.1316290110

Synthesis and assembly of fungal melanin
journal, December 2011

  • Eisenman, Helene C.; Casadevall, Arturo
  • Applied Microbiology and Biotechnology, Vol. 93, Issue 3
  • DOI: 10.1007/s00253-011-3777-2

The Target of β-Expansin EXPB1 in Maize Cell Walls from Binding and Solid-State NMR Studies
journal, October 2016

  • Wang, Tuo; Chen, Yuning; Tabuchi, Akira
  • Plant Physiology, Vol. 172, Issue 4
  • DOI: 10.1104/pp.16.01311

A simple method for quantitative determination of polysaccharides in fungal cell walls
journal, December 2006

Analysis of peptidogalactomannans from the mycelial surface of Aspergillus fumigatus
journal, January 1998

Invasive Aspergillosis
journal, August 2018

  • Darling, Bethany A.; Milder, Edmund A.
  • Pediatrics in Review, Vol. 39, Issue 9
  • DOI: 10.1542/pir.2017-0129

3D TEDOR NMR Experiments for the Simultaneous Measurement of Multiple Carbon−Nitrogen Distances in Uniformly 13 C, 15 N-Labeled Solids
journal, September 2002

  • Jaroniec, Christopher P.; Filip, Claudiu; Griffin, Robert G.
  • Journal of the American Chemical Society, Vol. 124, Issue 36
  • DOI: 10.1021/ja026385y

Aspergillosis Case-Fatality Rate: Systematic Review of the Literature
journal, February 2001

  • Lin, S. -J.; Schranz, J.; Teutsch, S. M.
  • Clinical Infectious Diseases, Vol. 32, Issue 3
  • DOI: 10.1086/318483

Cross polarization in the tilted frame: assignment and spectral simplification in heteronuclear spin systems
journal, December 1998

Microstructure of Cell Wall-Associated Melanin in the Human Pathogenic Fungus Cryptococcus neoformans †
journal, March 2005

  • Eisenman, Helene C.; Nosanchuk, Joshua D.; Webber, J. Beau W.
  • Biochemistry, Vol. 44, Issue 10
  • DOI: 10.1021/bi047731m

Aspergillus fumigatus melanins: interference with the host endocytosis pathway and impact on virulence
journal, January 2013

  • Heinekamp, Thorsten; Thywißen, Andreas; Macheleidt, Juliane
  • Frontiers in Microbiology, Vol. 3
  • DOI: 10.3389/fmicb.2012.00440

Invasive Aspergillosis
journal, April 1998

  • Denning, David W.
  • Clinical Infectious Diseases, Vol. 26, Issue 4
  • DOI: 10.1086/513943

A Trial of Itraconazole or Amphotericin B for HIV-Associated Talaromycosis
journal, June 2017

  • Le, Thuy; Kinh, Nguyen Van; Cuc, Ngo T. K.
  • New England Journal of Medicine, Vol. 376, Issue 24
  • DOI: 10.1056/NEJMoa1613306

Water Distribution, Dynamics, and Interactions with Alzheimer’s β-Amyloid Fibrils Investigated by Solid-State NMR
journal, April 2017

  • Wang, Tuo; Jo, Hyunil; DeGrado, William F.
  • Journal of the American Chemical Society, Vol. 139, Issue 17
  • DOI: 10.1021/jacs.7b02089

Infections with Aspergillus Species
journal, November 1993

β-glucan: Crucial component of the fungal cell wall and elusive MAMP in plants
journal, May 2016

Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization
journal, May 2017

  • Sergeyev, Ivan V.; Itin, Boris; Rogawski, Rivkah
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 20
  • DOI: 10.1073/pnas.1701484114

Exhaustive crystal structure search and crystal modeling of β-chitin
journal, March 2007

  • Yui, Toshifumi; Taki, Naoto; Sugiyama, Junji
  • International Journal of Biological Macromolecules, Vol. 40, Issue 4
  • DOI: 10.1016/j.ijbiomac.2006.08.017

Proton-Assisted Recoupling (PAR) in Peptides and Proteins
journal, November 2017

  • Donovan, Kevin J.; Jain, Sheetal K.; Silvers, Robert
  • The Journal of Physical Chemistry B, Vol. 121, Issue 48
  • DOI: 10.1021/acs.jpcb.7b08934

Antifungal agents: mechanisms of action
journal, June 2003

Polarization-Enhanced NMR Spectroscopy of Biomolecules in Frozen Solution
journal, May 1997

Surface hydrophobin prevents immune recognition of airborne fungal spores
journal, August 2009

  • Aimanianda, Vishukumar; Bayry, Jagadeesh; Bozza, Silvia
  • Nature, Vol. 460, Issue 7259
  • DOI: 10.1038/nature08264

Theoretical aspects of Magic Angle Spinning - Dynamic Nuclear Polarization
journal, September 2015

  • Mentink-Vigier, Frederic; Akbey, Ümit; Oschkinat, Hartmut
  • Journal of Magnetic Resonance, Vol. 258
  • DOI: 10.1016/j.jmr.2015.07.001

Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies
journal, January 2016

  • Gupta, Rupal; Lu, Manman; Hou, Guangjin
  • The Journal of Physical Chemistry B, Vol. 120, Issue 2
  • DOI: 10.1021/acs.jpcb.5b12134

Dynamic Nuclear Polarization Surface Enhanced NMR Spectroscopy
journal, March 2013

  • Rossini, Aaron J.; Zagdoun, Alexandre; Lelli, Moreno
  • Accounts of Chemical Research, Vol. 46, Issue 9
  • DOI: 10.1021/ar300322x

Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid-state NMR
journal, December 2016

  • Simmons, Thomas J.; Mortimer, Jenny C.; Bernardinelli, Oigres D.
  • Nature Communications, Vol. 7, Issue 1
  • DOI: 10.1038/ncomms13902

The refocused INADEQUATE MAS NMR experiment in multiple spin-systems: Interpreting observed correlation peaks and optimising lineshapes
journal, September 2007

  • Cadars, Sylvian; Sein, Julien; Duma, Luminita
  • Journal of Magnetic Resonance, Vol. 188, Issue 1
  • DOI: 10.1016/j.jmr.2007.05.016

Proton-Assisted Recoupling (PAR) in Peptides and Proteins
journal, November 2017

  • Donovan, Kevin J.; Jain, Sheetal K.; Silvers, Robert
  • The Journal of Physical Chemistry B, Vol. 121, Issue 48
  • DOI: 10.1021/acs.jpcb.7b08934

Architecture of the Yeast Cell Wall: β(1→6)-GLUCAN INTERCONNECTS MANNOPROTEIN, β(1→3)-GLUCAN, AND CHITIN
journal, July 1997

  • Kollár, Roman; Reinhold, Bruce B.; Petráková, Eva
  • Journal of Biological Chemistry, Vol. 272, Issue 28
  • DOI: 10.1074/jbc.272.28.17762

Rapid Natural-Abundance 2D 13 C- 13 C Correlation Spectroscopy Using Dynamic Nuclear Polarization Enhanced Solid-State NMR and Matrix-Free Sample Preparation
journal, October 2012

  • Takahashi, Hiroki; Lee, Daniel; Dubois, Lionel
  • Angewandte Chemie International Edition, Vol. 51, Issue 47
  • DOI: 10.1002/anie.201206102

Neurotoxin II Bound to Acetylcholine Receptors in Native Membranes Studied by Dynamic Nuclear Polarization NMR
journal, December 2011

  • Linden, Arne H.; Lange, Sascha; Franks, W. Trent
  • Journal of the American Chemical Society, Vol. 133, Issue 48
  • DOI: 10.1021/ja206999c

3D TEDOR NMR Experiments for the Simultaneous Measurement of Multiple Carbon−Nitrogen Distances in Uniformly 13 C, 15 N-Labeled Solids
journal, September 2002

  • Jaroniec, Christopher P.; Filip, Claudiu; Griffin, Robert G.
  • Journal of the American Chemical Society, Vol. 124, Issue 36
  • DOI: 10.1021/ja026385y

Microstructure of Cell Wall-Associated Melanin in the Human Pathogenic Fungus Cryptococcus neoformans †
journal, March 2005

  • Eisenman, Helene C.; Nosanchuk, Joshua D.; Webber, J. Beau W.
  • Biochemistry, Vol. 44, Issue 10
  • DOI: 10.1021/bi047731m

De novo determination of peptide structure with solid-state magic-angle spinning NMR spectroscopy
journal, July 2002

  • Rienstra, C. M.; Tucker-Kellogg, L.; Jaroniec, C. P.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 16
  • DOI: 10.1073/pnas.152346599

An even pattern of xylan substitution is critical for interaction with cellulose in plant cell walls
journal, October 2017

  • Grantham, Nicholas J.; Wurman-Rodrich, Joel; Terrett, Oliver M.
  • Nature Plants, Vol. 3, Issue 11
  • DOI: 10.1038/s41477-017-0030-8

Matrix-free dynamic nuclear polarization enables solid-state NMR 13C–13C correlation spectroscopy of proteins at natural isotopic abundance
journal, January 2013

  • Takahashi, Hiroki; Hediger, Sabine; De Paëpe, Gaël
  • Chemical Communications, Vol. 49, Issue 82
  • DOI: 10.1039/c3cc45195j

Polarization-Enhanced NMR Spectroscopy of Biomolecules in Frozen Solution
journal, May 1997

Hidden Killers: Human Fungal Infections
journal, December 2012

Synthesis and assembly of fungal melanin
journal, December 2011

  • Eisenman, Helene C.; Casadevall, Arturo
  • Applied Microbiology and Biotechnology, Vol. 93, Issue 3
  • DOI: 10.1007/s00253-011-3777-2

Relaxation-compensated difference spin diffusion NMR for detecting 13C–13C long-range correlations in proteins and polysaccharides
journal, December 2014

  • Wang, Tuo; Williams, Jonathan K.; Schmidt-Rohr, Klaus
  • Journal of Biomolecular NMR, Vol. 61, Issue 2
  • DOI: 10.1007/s10858-014-9889-0

Cell Wall Chitosan Is Necessary for Virulence in the Opportunistic Pathogen Cryptococcus neoformans
journal, July 2011

  • Baker, Lorina G.; Specht, Charles A.; Lodge, Jennifer K.
  • Eukaryotic Cell, Vol. 10, Issue 9
  • DOI: 10.1128/ec.05138-11

Therapeutic Outcome in Invasive Aspergillosis
journal, September 1996

Cell Wall Architecture in Yeast: New Structure and New Challenges
journal, August 1998

Highly Efficient, Water-Soluble Polarizing Agents for Dynamic Nuclear Polarization at High Frequency
journal, August 2013

  • Sauvée, Claire; Rosay, Melanie; Casano, Gilles
  • Angewandte Chemie, Vol. 125, Issue 41
  • DOI: 10.1002/ange.201304657

Chitosan: An Update on Potential Biomedical and Pharmaceutical Applications
journal, August 2015

  • Cheung, Randy; Ng, Tzi; Wong, Jack
  • Marine Drugs, Vol. 13, Issue 8
  • DOI: 10.3390/md13085156

Phosphoethanolamine cellulose: A naturally produced chemically modified cellulose
journal, January 2018

  • Thongsomboon, Wiriya; Serra, Diego O.; Possling, Alexandra
  • Science, Vol. 359, Issue 6373
  • DOI: 10.1126/science.aao4096

15 N− 15 N Proton Assisted Recoupling in Magic Angle Spinning NMR
journal, April 2009

  • Lewandowski, Józef R.; Paëpe, Gaël De; Eddy, Matthew T.
  • Journal of the American Chemical Society, Vol. 131, Issue 16
  • DOI: 10.1021/ja806578y

Molecular organization of the alkali-insoluble fraction of Aspergillus fumigatus cell wall.
journal, December 2000

    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    Evidence for ligninolytic activity of the ascomycete fungus Podospora anserina
    journal, April 2020

    • van Erven, Gijs; Kleijn, Anne F.; Patyshakuliyeva, Aleksandrina
    • Biotechnology for Biofuels, Vol. 13, Issue 1
    • DOI: 10.1186/s13068-020-01713-z

    Molecular Mass and Localization of α-1,3-Glucan in Cell Wall Control the Degree of Hyphal Aggregation in Liquid Culture of Aspergillus nidulans
    journal, November 2018

    Chitinous polymers: extraction from fungal sources, characterization and processing towards value‐added applications
    journal, May 2020

    • Araújo, Diana; Ferreira, Inês C.; Torres, Cristiana AV
    • Journal of Chemical Technology & Biotechnology, Vol. 95, Issue 5
    • DOI: 10.1002/jctb.6325

    Atomic resolution of cotton cellulose structure enabled by dynamic nuclear polarization solid-state NMR
    journal, November 2018

    Molecular architecture of softwood revealed by solid-state NMR
    journal, October 2019

    Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR
    journal, January 2019

    Longitudinal Relaxation Optimization Enhances 1 H‐Detected HSQC in Solid‐State NMR Spectroscopy on Challenging Biological Systems
    journal, February 2019

    • Han, Rong; Yang, Yufei; Wang, Shenlin
    • Chemistry – A European Journal, Vol. 25, Issue 16
    • DOI: 10.1002/chem.201805327

    Circulating (1→3)-β-D-glucan Is Associated With Immune Activation During Human Immunodeficiency Virus Infection
    journal, March 2019

    • Mehraj, Vikram; Ramendra, Rayoun; Isnard, Stéphane
    • Clinical Infectious Diseases, Vol. 70, Issue 2
    • DOI: 10.1093/cid/ciz212

    Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR
    journal, January 2019

    Molecular architecture of softwood revealed by solid-state NMR
    text, January 2019

    • Terrett, Oliver M.; Lyczakowski, Jan J.; Yu, Li
    • Apollo - University of Cambridge Repository
    • DOI: 10.17863/cam.59388

    Aspergillus fumigatus and Aspergillosis in 2019
    journal, November 2019

    • Latgé, Jean-Paul; Chamilos, Georgios
    • Clinical Microbiology Reviews, Vol. 33, Issue 1
    • DOI: 10.1128/cmr.00140-18

    The puzzling construction of the conidial outer layer of Aspergillus fumigatus
    journal, January 2019

    • Valsecchi, Isabel; Dupres, Vincent; Michel, Jean‐Philippe
    • Cellular Microbiology, Vol. 21, Issue 5
    • DOI: 10.1111/cmi.12994

    Integration of electron microscopy and solid-state NMR analysis for new views and compositional parameters of Aspergillus fumigatus biofilms
    journal, February 2019

    • Reichhardt, Courtney; Joubert, Lydia-Marie; Clemons, Karl V.
    • Medical Mycology, Vol. 57, Issue Supplement_2
    • DOI: 10.1093/mmy/myy140

    FGB1 and WSC3 are in planta‐ induced β ‐glucan‐binding fungal lectins with different functions
    journal, February 2019

    • Wawra, Stephan; Fesel, Philipp; Widmer, Heidi
    • New Phytologist, Vol. 222, Issue 3
    • DOI: 10.1111/nph.15711

    The melanization road more traveled by: Precursor substrate effects on melanin synthesis in cell-free and fungal cell systems
    journal, November 2018

    • Chatterjee, Subhasish; Prados-Rosales, Rafael; Tan, Sindy
    • Journal of Biological Chemistry, Vol. 293, Issue 52
    • DOI: 10.1074/jbc.ra118.005791

    Molecular architecture of softwood revealed by solid-state NMR.
    text, January 2019

    • Terrett, Oliver; Lyczakowski, Jan; Yu, Li
    • Apollo - University of Cambridge Repository
    • DOI: 10.17863/cam.44837
      Sort by:
      [ × clear filter / sort ]

      Similar Records in DOE PAGES and OSTI.GOV collections: