Cloud-Enabled Microscopy and Droplet Microfluidic Platform for Specific Detection of Escherichia coli in Water
Abstract
We report an all-in-one platform – ScanDrop – for the rapid and specific capture, detection, and identification of bacteria in drinking water. The ScanDrop platform integrates droplet microfluidics, a portable imaging system, and cloud-based control software and data storage. The cloud-based control software and data storage enables robotic image acquisition, remote image processing, and rapid data sharing. These features form a “cloud” network for water quality monitoring. We have demonstrated the capability of ScanDrop to perform water quality monitoring via the detection of an indicator coliform bacterium, Escherichia coli, in drinking water contaminated with feces. Magnetic beads conjugated with antibodies to E. coli antigen were used to selectively capture and isolate specific bacteria from water samples. The bead-captured bacteria were co-encapsulated in pico-liter droplets with fluorescently-labeled anti-E. coli antibodies, and imaged with an automated custom designed fluorescence microscope. The entire water quality diagnostic process required 8 hours from sample collection to online-accessible results compared with 2–4 days for other currently available standard detection methods.
- Authors:
-
- Harvard Medical School, Boston, MA (United States). Shriners Burns Inst. Massachusetts General Hospital. Centre for Engineering in Medicine
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States). Fuels Synthesis Division; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical BioSciences Division; USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- Technion-Israel Inst. of Technology, Haifa (Israel). Dept. of Computer Science
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States). Fuels Synthesis Division; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical BioSciences Division
- Harvard Medical School, Boston, MA (United States). Shriners Burns Inst. Massachusetts General Hospital. Centre for Engineering in Medicine; Rutgers Univ., New Brunswick, NJ (United States). Dept. of Biomedical Engineering
- Ben-Gurion Univ. of the Negev, Beer-Sheva (Israel). The National Inst. of Biotechnology in Negev. Dept. of Biotechnology Engineering; Nanyang Technological Univ. (Singapore). School of Materials Science and Engineering; NRF CREATE program for Nanomaterials in Energy and Water Management (Singapore)
- Northeastern Univ., Boston, MA (United States). Dept. of Pharmaceutical Sciences. School of Pharmacy. Bouvé College of Health Sciences
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); Shriners Foundation; Singapore National Research Foundation
- OSTI Identifier:
- 1511391
- Grant/Contract Number:
- AC02-05CH11231; 85120-BOS
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; microfluidics; water resources; polymerase chain reaction; water pollution; water quality; fluorescence microscopy; fluorescence imaging; water management
Citation Formats
Golberg, Alexander, Linshiz, Gregory, Kravets, Ilia, Stawski, Nina, Hillson, Nathan J., Yarmush, Martin L., Marks, Robert S., and Konry, Tania. Cloud-Enabled Microscopy and Droplet Microfluidic Platform for Specific Detection of Escherichia coli in Water. United States: N. p., 2014.
Web. doi:10.1371/journal.pone.0086341.
Golberg, Alexander, Linshiz, Gregory, Kravets, Ilia, Stawski, Nina, Hillson, Nathan J., Yarmush, Martin L., Marks, Robert S., & Konry, Tania. Cloud-Enabled Microscopy and Droplet Microfluidic Platform for Specific Detection of Escherichia coli in Water. United States. https://doi.org/10.1371/journal.pone.0086341
Golberg, Alexander, Linshiz, Gregory, Kravets, Ilia, Stawski, Nina, Hillson, Nathan J., Yarmush, Martin L., Marks, Robert S., and Konry, Tania. Mon .
"Cloud-Enabled Microscopy and Droplet Microfluidic Platform for Specific Detection of Escherichia coli in Water". United States. https://doi.org/10.1371/journal.pone.0086341. https://www.osti.gov/servlets/purl/1511391.
@article{osti_1511391,
title = {Cloud-Enabled Microscopy and Droplet Microfluidic Platform for Specific Detection of Escherichia coli in Water},
author = {Golberg, Alexander and Linshiz, Gregory and Kravets, Ilia and Stawski, Nina and Hillson, Nathan J. and Yarmush, Martin L. and Marks, Robert S. and Konry, Tania},
abstractNote = {We report an all-in-one platform – ScanDrop – for the rapid and specific capture, detection, and identification of bacteria in drinking water. The ScanDrop platform integrates droplet microfluidics, a portable imaging system, and cloud-based control software and data storage. The cloud-based control software and data storage enables robotic image acquisition, remote image processing, and rapid data sharing. These features form a “cloud” network for water quality monitoring. We have demonstrated the capability of ScanDrop to perform water quality monitoring via the detection of an indicator coliform bacterium, Escherichia coli, in drinking water contaminated with feces. Magnetic beads conjugated with antibodies to E. coli antigen were used to selectively capture and isolate specific bacteria from water samples. The bead-captured bacteria were co-encapsulated in pico-liter droplets with fluorescently-labeled anti-E. coli antibodies, and imaged with an automated custom designed fluorescence microscope. The entire water quality diagnostic process required 8 hours from sample collection to online-accessible results compared with 2–4 days for other currently available standard detection methods.},
doi = {10.1371/journal.pone.0086341},
journal = {PLoS ONE},
number = 1,
volume = 9,
place = {United States},
year = {Mon Jan 27 00:00:00 EST 2014},
month = {Mon Jan 27 00:00:00 EST 2014}
}
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Figures / Tables:
Figure 1: Bacteria capturing and detection assay. Magnetic bead capture of E. coli from enriched water samples, and downstream chip encapsulation for fluorescent labeling and detection. 1L of water is passed through a 0.22 mm filter, which is then incubated for 6 hr in LB media. Dynabeads® MAX anti-E. colimore »
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Works referenced in this record:
Water and sewage systems, socio-demographics, and duration of residence associated with endemic intestinal infectious diseases: A cohort study
journal, December 2010
- Teschke, Kay; Bellack, Neil; Shen, Hui
- BMC Public Health, Vol. 10, Issue 1
Digital Microfluidics
journal, July 2012
- Choi, Kihwan; Ng, Alphonsus H. C.; Fobel, Ryan
- Annual Review of Analytical Chemistry, Vol. 5, Issue 1
Estimating the Burden of Disease from Water, Sanitation, and Hygiene at a Global Level
journal, April 2002
- Prüss, Annette; Kay, David; Fewtrell, Lorna
- Environmental Health Perspectives, Vol. 110, Issue 5
Fountain Flow™ cytometry, a new technique for the rapid detection and enumeration of microorganisms in aqueous samples
journal, January 2006
- Johnson, Paul E.; Deromedi, Anthony J.; Lebaron, Philippe
- Cytometry Part A, Vol. 69A, Issue 12
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems
book, January 2008
- Zourob, Mohammed; Elwary, Souna; Turner, Anthony
Nano-silver-modified PQC/DNA biosensor for detecting E. coli in environmental water
journal, January 2009
- Sun, H.; Choy, T. S.; Zhu, D. R.
- Biosensors and Bioelectronics, Vol. 24, Issue 5
Quantitative analysis of microbial contamination in private drinking water supply systems
journal, April 2013
- Allevi, Richard P.; Krometis, Leigh-Anne H.; Hagedorn, Charles
- Journal of Water and Health, Vol. 11, Issue 2
Microspot sensing based on surface-enhanced fluorescence from nanosculptured thin films
journal, January 2012
- Karabchevsky, Alina
- Journal of Nanophotonics, Vol. 6, Issue 1
Development and use of 16S rRNA gene targeted PCR primers for the identification of Escherichia coli cells in water
journal, September 1998
- Tsen, H. Y.; Lin, C. K.; Chi, W. R.
- Journal of Applied Microbiology, Vol. 85, Issue 3
Single-cell analysis and sorting using droplet-based microfluidics
journal, April 2013
- Mazutis, Linas; Gilbert, John; Ung, W. Lloyd
- Nature Protocols, Vol. 8, Issue 5
Detection of viable but nonculturable Escherichia coli O157:H7 using propidium monoazide treatments and qPCR
journal, March 2013
- Xiao, Xing-long; Tian, Cong; Yu, Yi-gang
- Canadian Journal of Microbiology, Vol. 59, Issue 3
Highly sensitive amperometric immunosensor for the detection of Escherichia coli
journal, August 2009
- Abu-Rabeah, K.; Ashkenazi, A.; Atias, D.
- Biosensors and Bioelectronics, Vol. 24, Issue 12
Micro Total Analysis Systems. 1. Introduction, Theory, and Technology
journal, June 2002
- Reyes, Darwin R.; Iossifidis, Dimitri; Auroux, Pierre-Alain
- Analytical Chemistry, Vol. 74, Issue 12
Droplet microfluidics for high-throughput biological assays
journal, January 2012
- Guo, Mira T.; Rotem, Assaf; Heyman, John A.
- Lab on a Chip, Vol. 12, Issue 12
Escherichia coli: the best biological drinking water indicator for public health protection
journal, December 2000
- Edberg, S. C.; Rice, E. W.; Karlin, R. J.
- Journal of Applied Microbiology, Vol. 88, Issue S1
Detection and enumeration of coliforms in drinking water: current methods and emerging approaches
journal, March 2002
- Rompré, Annie; Servais, Pierre; Baudart, Julia
- Journal of Microbiological Methods, Vol. 49, Issue 1
The social ecology of water in a Mumbai slum: failures in water quality, quantity, and reliability
journal, February 2013
- Subbaraman, Ramnath; Shitole, Shrutika; Shitole, Tejal
- BMC Public Health, Vol. 13, Issue 1
Sensitivity-enhancement methods for surface plasmon sensors
journal, March 2011
- Shalabney, A.; Abdulhalim, I.
- Laser & Photonics Reviews, Vol. 5, Issue 4
PaR-PaR Laboratory Automation Platform
journal, October 2012
- Linshiz, Gregory; Stawski, Nina; Poust, Sean
- ACS Synthetic Biology, Vol. 2, Issue 5
Mathematical modeling of drop mixing in a slit-type microchannel
journal, August 2001
- Handique, K.; Burns, M. A.
- Journal of Micromechanics and Microengineering, Vol. 11, Issue 5
Gastroenterology in developing countries: Issues and advances
journal, January 2009
- Mandeville, Kate L.; Krabshuis, Justus; Ladep, Nimzing Gwamzhi
- World Journal of Gastroenterology, Vol. 15, Issue 23
Immobilization strategies of Brucella particles on optical fibers for use in chemiluminescence immunosensors
journal, November 2009
- Liebes, Yael; Amir, Liron; Marks, Robert S.
- Talanta, Vol. 80, Issue 1
Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens
journal, January 2012
- Setterington, Emma B.; Alocilja, Evangelyn C.
- Biosensors, Vol. 2, Issue 1
Dynamics of microfluidic droplets
journal, January 2010
- Baroud, Charles N.; Gallaire, Francois; Dangla, Rémi
- Lab on a Chip, Vol. 10, Issue 16
A review of technologies for rapid detection of bacteria in recreational waters
journal, December 2005
- Noble, Rachel T.; Weisberg, Stephen B.
- Journal of Water and Health, Vol. 3, Issue 4
Mixing characterization inside microdroplets engineered on a microcoalescer
journal, February 2007
- Sarrazin, F.; Prat, L.; Di Miceli, N.
- Chemical Engineering Science, Vol. 62, Issue 4
Extreme water-related weather events and waterborne disease
journal, August 2012
- Cann, K. F.; Thomas, D. Rh.; Salmon, R. L.
- Epidemiology and Infection, Vol. 141, Issue 4
Design, implementation and practice of JBEI-ICE: an open source biological part registry platform and tools
journal, June 2012
- Ham, T. S.; Dmytriv, Z.; Plahar, H.
- Nucleic Acids Research, Vol. 40, Issue 18
Global Distribution of Outbreaks of Water-Associated Infectious Diseases
journal, February 2012
- Yang, Kun; LeJeune, Jeffrey; Alsdorf, Doug
- PLoS Neglected Tropical Diseases, Vol. 6, Issue 2
Total viable bacterial count using a real time all-fibre spectroscopic system
journal, January 2013
- Bogomolny, E.; Swift, S.; Vanholsbeeck, F.
- The Analyst, Vol. 138, Issue 14
Surface-enhanced fluorescence from metal sculptured thin films with application to biosensing in water
journal, February 2009
- Abdulhalim, I.; Karabchevsky, Alina; Patzig, Christian
- Applied Physics Letters, Vol. 94, Issue 6
Rethinking Indicators of Microbial Drinking Water Quality for Health Studies in Tropical Developing Countries: Case Study in Northern Coastal Ecuador
journal, March 2012
- Nelson, Kara L.; Levy, Karen; Eisenberg, Joseph N. S.
- The American Journal of Tropical Medicine and Hygiene, Vol. 86, Issue 3
Microfluidic Systems for Pathogen Sensing: A Review
journal, June 2009
- Mairhofer, Jürgen; Roppert, Kriemhilt; Ertl, Peter
- Sensors, Vol. 9, Issue 6
Chemiluminescent optical fiber immunosensor detection of Brucella cells presenting smooth-A antigen
journal, July 2009
- Liebes, Yael; Marks, Robert S.; Banai, Menachem
- Sensors and Actuators B: Chemical, Vol. 140, Issue 2
Burden of childhood diseases and malnutrition in a semi-urban slum in southern India
journal, January 2013
- Sarkar, Rajiv; Sivarathinaswamy, Prabhu; Thangaraj, Bhuvaneshwari
- BMC Public Health, Vol. 13, Issue 1
Fountain Flow™ cytometry, a new technique for the rapid detection and enumeration of microorganisms in aqueous samples
journal, January 2006
- Johnson, Paul E.; Deromedi, Anthony J.; Lebaron, Philippe
- Cytometry Part A, Vol. 69A, Issue 12
Sensitivity-enhancement methods for surface plasmon sensors
journal, March 2011
- Shalabney, A.; Abdulhalim, I.
- Laser & Photonics Reviews, Vol. 5, Issue 4
Nano-silver-modified PQC/DNA biosensor for detecting E. coli in environmental water
journal, January 2009
- Sun, H.; Choy, T. S.; Zhu, D. R.
- Biosensors and Bioelectronics, Vol. 24, Issue 5
Highly sensitive amperometric immunosensor for the detection of Escherichia coli
journal, August 2009
- Abu-Rabeah, K.; Ashkenazi, A.; Atias, D.
- Biosensors and Bioelectronics, Vol. 24, Issue 12
Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms
journal, August 2008
- Clausell-Tormos, Jenifer; Lieber, Diana; Baret, Jean-Christophe
- Chemistry & Biology, Vol. 15, Issue 8
Chemiluminescent optical fiber immunosensor detection of Brucella cells presenting smooth-A antigen
journal, July 2009
- Liebes, Yael; Marks, Robert S.; Banai, Menachem
- Sensors and Actuators B: Chemical, Vol. 140, Issue 2
Immobilization strategies of Brucella particles on optical fibers for use in chemiluminescence immunosensors
journal, November 2009
- Liebes, Yael; Amir, Liron; Marks, Robert S.
- Talanta, Vol. 80, Issue 1
Micro Total Analysis Systems. 1. Introduction, Theory, and Technology
journal, June 2002
- Reyes, Darwin R.; Iossifidis, Dimitri; Auroux, Pierre-Alain
- Analytical Chemistry, Vol. 74, Issue 12
PaR-PaR Laboratory Automation Platform
journal, October 2012
- Linshiz, Gregory; Stawski, Nina; Poust, Sean
- ACS Synthetic Biology, Vol. 2, Issue 5
Building droplet-based microfluidic systems for biological analysis
journal, July 2012
- Niu, Xize; deMello, Andrew J.
- Biochemical Society Transactions, Vol. 40, Issue 4
Development and use of 16S rRNA gene targeted PCR primers for the identification of Escherichia coli cells in water
journal, September 1998
- Tsen, H. Y.; Lin, C. K.; Chi, W. R.
- Journal of Applied Microbiology, Vol. 85, Issue 3
Microfluidic systems for chemical kinetics that rely on chaotic mixing in droplets
journal, May 2004
- Bringer, Michelle R.; Gerdts, Cory J.; Song, Helen
- Philosophical Transactions of the Royal Society of London. Series A: Mathematical, Physical and Engineering Sciences, Vol. 362, Issue 1818
Escherichia coli: the best biological drinking water indicator for public health protection
journal, December 2000
- Edberg, S. C.; Rice, E. W.; Karlin, R. J.
- Journal of Applied Microbiology, Vol. 88, Issue S1
Detection of Viable but Nonculturable Escherichia coli O157:H7 Bacteria in Drinking Water and River Water
journal, January 2008
- Liu, Y.; Gilchrist, A.; Zhang, J.
- Applied and Environmental Microbiology, Vol. 74, Issue 5
Selective and Sensitive Method for PCR Amplification of
Escherichia coli
16S rRNA Genes in Soil
journal, February 2000
- Sabat, G.; Rose, P.; Hickey, W. J.
- Applied and Environmental Microbiology, Vol. 66, Issue 2
Detection of viable but nonculturable Escherichia coli O157:H7 using propidium monoazide treatments and qPCR
journal, March 2013
- Xiao, Xing-long; Tian, Cong; Yu, Yi-gang
- Canadian Journal of Microbiology, Vol. 59, Issue 3
Digital Microfluidics
journal, July 2012
- Choi, Kihwan; Ng, Alphonsus H. C.; Fobel, Ryan
- Annual Review of Analytical Chemistry, Vol. 5, Issue 1
Bacteriological and Physicochemical Studies on Tigris River Near the Water Purification Stations within Baghdad Province
journal, January 2012
- Al-Bayatti, Khalid K.; Al-Arajy, Kadhum H.; Al-Nuaemy, Seba Hussain
- Journal of Environmental and Public Health, Vol. 2012
Water and sewage systems, socio-demographics, and duration of residence associated with endemic intestinal infectious diseases: A cohort study
journal, December 2010
- Teschke, Kay; Bellack, Neil; Shen, Hui
- BMC Public Health, Vol. 10, Issue 1
Estimating the burden of disease from water, sanitation, and hygiene at a global level.
journal, May 2002
- Prüss, Annette; Kay, David; Fewtrell, Lorna
- Environmental Health Perspectives, Vol. 110, Issue 5
A review of technologies for rapid detection of bacteria in recreational waters
journal, December 2005
- Noble, Rachel T.; Weisberg, Stephen B.
- Journal of Water and Health, Vol. 3, Issue 4
Quantitative analysis of microbial contamination in private drinking water supply systems
journal, April 2013
- Allevi, Richard P.; Krometis, Leigh-Anne H.; Hagedorn, Charles
- Journal of Water and Health, Vol. 11, Issue 2
Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens
journal, January 2012
- Setterington, Emma B.; Alocilja, Evangelyn C.
- Biosensors, Vol. 2, Issue 1
Microfluidic Systems for Pathogen Sensing: A Review
journal, June 2009
- Mairhofer, Jürgen; Roppert, Kriemhilt; Ertl, Peter
- Sensors, Vol. 9, Issue 6
Gastroenterology in developing countries: Issues and advances
journal, January 2009
- Mandeville, Kate L.; Krabshuis, Justus; Ladep, Nimzing Gwamzhi
- World Journal of Gastroenterology, Vol. 15, Issue 23
Works referencing / citing this record:
Recent trends in the detection of pathogenic Escherichia coli O157 : H7
journal, August 2015
- Wu, Si-Ying; Hulme, John; An, Seong Soo A.
- BioChip Journal, Vol. 9, Issue 3
Highly sensitive and specific detection of E. coli by a SERS nanobiosensor chip utilizing metallic nanosculptured thin films
journal, January 2015
- Srivastava, Sachin K.; Hamo, Hilla Ben; Kushmaro, Ariel
- The Analyst, Vol. 140, Issue 9
Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution of in-vitro liver technologies
journal, March 2015
- Usta, O. B.; McCarty, W. J.; Bale, S.
- TECHNOLOGY, Vol. 03, Issue 01
Development of a Microsphere-Based System to Facilitate Real-Time Insulin Monitoring
journal, December 2015
- Kahanovitz, Lindy; Seker, Erkin; Marks, Robert S.
- Journal of Diabetes Science and Technology, Vol. 10, Issue 3
Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
journal, January 2018
- Gutiérrez-del-Río, Ignacio; Marín, Laura; Fernández, Javier
- PLOS ONE, Vol. 13, Issue 1
Recent Advances in Applications of Droplet Microfluidics
journal, September 2015
- Chou, Wei-Lung; Lee, Pee-Yew; Yang, Cing-Long
- Micromachines, Vol. 6, Issue 9
Microfluidics Integrated Biosensors: A Leading Technology towards Lab-on-a-Chip and Sensing Applications
journal, December 2015
- Luka, George; Ahmadi, Ali; Najjaran, Homayoun
- Sensors, Vol. 15, Issue 12
A Comprehensive Review of Microfluidic Water Quality Monitoring Sensors
journal, November 2019
- Jaywant, Swapna A.; Arif, Khalid Mahmood
- Sensors, Vol. 19, Issue 21
Microfluidics Integrated Biosensors: A Leading Technology towards Lab-on-a-Chip and Sensing Applications
text, January 2019
- Luka, George; Ahmadi, Ali; Najjaran, Homayoun
- Multidisciplinary Digital Publishing Institute
Punch Card Programmable Microfluidics
journal, March 2015
- Korir, George; Prakash, Manu
- PLOS ONE, Vol. 10, Issue 3
Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water
journal, January 2018
- Gutiérrez-del-Río, Ignacio; Marín, Laura; Fernández, Javier
- PLOS ONE, Vol. 13, Issue 1
Lab-on-Chip Cytometry Based on Magnetoresistive Sensors for Bacteria Detection in Milk
journal, August 2014
- Fernandes, Ana; Duarte, Carla; Cardoso, Filipe
- Sensors, Vol. 14, Issue 8
Microfluidics Integrated Biosensors: A Leading Technology towards Lab-on-a-Chip and Sensing Applications
journal, December 2015
- Luka, George; Ahmadi, Ali; Najjaran, Homayoun
- Sensors, Vol. 15, Issue 12
A Comprehensive Review of Microfluidic Water Quality Monitoring Sensors
journal, November 2019
- Jaywant, Swapna A.; Arif, Khalid Mahmood
- Sensors, Vol. 19, Issue 21
T Cell Dynamic Activation and Functional Analysis in Nanoliter Droplet Microarray
journal, January 2015
- Sarkar, Saheli
- Journal of Clinical & Cellular Immunology, Vol. 06, Issue 03
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