Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation
Abstract
The Mediator complex is a central component of transcriptional regulation in Eukaryotes. The complex is structurally divided into four modules known as the head, middle, tail and kinase modules, and in Arabidopsis thaliana, comprises 28-34 subunits. Here, we explore the functions of four Arabidopsis Medi-ator tail subunits, MED2, MED5a/b, MED16, and MED23, by comparing the impact of mutations in each on the Arabidopsis transcriptome. We find that these subunits affect both unique and overlapping sets of genes, providing insight into the functional and structural relationships between them. The mutants pri-marily exhibit changes in the expression of genes related to biotic and abiotic stress. We find evidence for a tissue specific role for MED23, as well as in the production of alternative transcripts. Together, our data help disentangle the individual contributions of these MED subunits to global gene expression and suggest new avenues for future research into their functions.
- Authors:
-
[1];
[1]
- Dept. of Biochemistry and Purdue Center for Plant Biology, West Lafayette, IN (United States)
- Publication Date:
- Research Org.:
- Purdue Univ., West Lafayette, IN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1510783
- Grant/Contract Number:
- FG02-07ER15905
- Resource Type:
- Accepted Manuscript
- Journal Name:
- G3
- Additional Journal Information:
- Journal Volume: 8; Journal Issue: 9; Journal ID: ISSN 2160-1836
- Publisher:
- Genetics Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Mediator; Arabidopsis; transcription regulation; gene expression
Citation Formats
Dolan, Whitney L., and Chapple, Clint. Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation. United States: N. p., 2018.
Web. doi:10.1534/g3.118.200573.
Dolan, Whitney L., & Chapple, Clint. Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation. United States. https://doi.org/10.1534/g3.118.200573
Dolan, Whitney L., and Chapple, Clint. Thu .
"Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation". United States. https://doi.org/10.1534/g3.118.200573. https://www.osti.gov/servlets/purl/1510783.
@article{osti_1510783,
title = {Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation},
author = {Dolan, Whitney L. and Chapple, Clint},
abstractNote = {The Mediator complex is a central component of transcriptional regulation in Eukaryotes. The complex is structurally divided into four modules known as the head, middle, tail and kinase modules, and in Arabidopsis thaliana, comprises 28-34 subunits. Here, we explore the functions of four Arabidopsis Medi-ator tail subunits, MED2, MED5a/b, MED16, and MED23, by comparing the impact of mutations in each on the Arabidopsis transcriptome. We find that these subunits affect both unique and overlapping sets of genes, providing insight into the functional and structural relationships between them. The mutants pri-marily exhibit changes in the expression of genes related to biotic and abiotic stress. We find evidence for a tissue specific role for MED23, as well as in the production of alternative transcripts. Together, our data help disentangle the individual contributions of these MED subunits to global gene expression and suggest new avenues for future research into their functions.},
doi = {10.1534/g3.118.200573},
journal = {G3},
number = 9,
volume = 8,
place = {United States},
year = {Thu Jul 26 00:00:00 EDT 2018},
month = {Thu Jul 26 00:00:00 EDT 2018}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 12 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Mediator structure and rearrangements required for holoenzyme formation
journal, March 2017
- Tsai, Kuang-Lei; Yu, Xiaodi; Gopalan, Sneha
- Nature, Vol. 544, Issue 7649
A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II
journal, May 1994
- Kim, Young-Joon; Björklund, Stefan; Li, Yang
- Cell, Vol. 77, Issue 4
The Mediator complex subunits MED25/PFT1 and MED8 are required for transcriptional responses to changes in cell wall arabinose composition and glucose treatment in Arabidopsis thaliana
journal, September 2015
- Seguela-Arnaud, Mathilde; Smith, Caroline; Uribe, Marcos Castellanos
- BMC Plant Biology, Vol. 15, Issue 1
Mediator: A key regulator of plant development
journal, November 2016
- Buendía-Monreal, Manuel; Gillmor, C. Stewart
- Developmental Biology, Vol. 419, Issue 1
CDK8 is a positive regulator of transcriptional elongation within the serum response network
journal, January 2010
- Donner, Aaron J.; Ebmeier, Christopher C.; Taatjes, Dylan J.
- Nature Structural & Molecular Biology, Vol. 17, Issue 2
Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
journal, September 2011
- Xu, R.; Li, Y.
- Development, Vol. 138, Issue 20
The Arabidopsis Mediator Subunit MED25 Differentially Regulates Jasmonate and Abscisic Acid Signaling through Interacting with the MYC2 and ABI5 Transcription Factors
journal, July 2012
- Chen, Rong; Jiang, Hongling; Li, Lin
- The Plant Cell, Vol. 24, Issue 7
Complexity of the Alternative Splicing Landscape in Plants
journal, October 2013
- Reddy, Anireddy S. N.; Marquez, Yamile; Kalyna, Maria
- The Plant Cell, Vol. 25, Issue 10
PHYTOCHROME AND FLOWERING TIME1/MEDIATOR25 Regulates Lateral Root Formation via Auxin Signaling in Arabidopsis
journal, April 2014
- Raya-González, Javier; Ortiz-Castro, Randy; Ruíz-Herrera, León Francisco
- Plant Physiology, Vol. 165, Issue 2
ImageJ2: ImageJ for the next generation of scientific image data
journal, November 2017
- Rueden, Curtis T.; Schindelin, Johannes; Hiner, Mark C.
- BMC Bioinformatics, Vol. 18, Issue 1
TopHat: discovering splice junctions with RNA-Seq
journal, March 2009
- Trapnell, Cole; Pachter, Lior; Salzberg, Steven L.
- Bioinformatics, Vol. 25, Issue 9
The Arabidopsis Mediator Complex Subunit16 Positively Regulates Salicylate-Mediated Systemic Acquired Resistance and Jasmonate/Ethylene-Induced Defense Pathways
journal, October 2012
- Zhang, Xudong; Wang, Chenggang; Zhang, Yanping
- The Plant Cell, Vol. 24, Issue 10
A novel mediator between activator proteins and the RNA polymerase II transcription apparatus
journal, June 1990
- Kelleher, Raymond J.; Flanagan, Peter M.; Kornberg, Roger D.
- Cell, Vol. 61, Issue 7
An Activator Binding Module of Yeast RNA Polymerase II Holoenzyme
journal, April 1999
- Lee, Young Chul; Park, Jin Mo; Min, Soyoung
- Molecular and Cellular Biology, Vol. 19, Issue 4
The Arabidopsis Mediator complex subunits MED14/SWP and MED16/SFR6/IEN1 differentially regulate defense gene expression in plant immune responses
journal, May 2013
- Zhang, Xudong; Yao, Jin; Zhang, Yanping
- The Plant Journal, Vol. 75, Issue 3
ATTED-II in 2016: A Plant Coexpression Database Towards Lineage-Specific Coexpression
journal, November 2015
- Aoki, Yuichi; Okamura, Yasunobu; Tadaka, Shu
- Plant and Cell Physiology, Vol. 57, Issue 1
Conservation and Divergence of Mediator Structure and Function: Insights from Plants
journal, November 2016
- Dolan, Whitney L.; Chapple, Clint
- Plant and Cell Physiology
The M ediator subunit MED 23 couples H2B mono‐ubiquitination to transcriptional control and cell fate determination
journal, September 2015
- Yao, Xiao; Tang, Zhanyun; Fu, Xing
- The EMBO Journal, Vol. 34, Issue 23
Mediator Expression Profiling Epistasis Reveals a Signal Transduction Pathway with Antagonistic Submodules and Highly Specific Downstream Targets
journal, August 2005
- van de Peppel, Jeroen; Kettelarij, Nienke; van Bakel, Harm
- Molecular Cell, Vol. 19, Issue 4
Mediator MED23 regulates basal transcription in vivo via an interaction with P-TEFb
journal, January 2013
- Wang, Wei; Yao, Xiao; Huang, Yan
- Transcription, Vol. 4, Issue 1
Alternative splicing of the auxin biosynthesis gene YUCCA4 determines its subcellular compartmentation: YUCCA4 and auxin biosynthesis
journal, January 2012
- Kriechbaumer, Verena; Wang, Pengwei; Hawes, Chris
- The Plant Journal, Vol. 70, Issue 2
Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme.
journal, November 1995
- Li, Y.; Bjorklund, S.; Jiang, Y. W.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 24
Comparative genomics supports a deep evolutionary origin for the large, four-module transcriptional mediator complex
journal, May 2008
- Bourbon, Henri-Marc
- Nucleic Acids Research, Vol. 36, Issue 12
The Mediator Complex Subunit PFT1 Is a Key Regulator of Jasmonate-Dependent Defense in Arabidopsis
journal, August 2009
- Kidd, Brendan N.; Edgar, Cameron I.; Kumar, Krish K.
- The Plant Cell, Vol. 21, Issue 8
Architecture of the RNA polymerase II–Mediator core initiation complex
journal, February 2015
- Plaschka, C.; Larivière, L.; Wenzeck, L.
- Nature, Vol. 518, Issue 7539
A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis
journal, March 2013
- Remy, Estelle; Cabrito, Tânia R.; Baster, Pawel
- The Plant Cell, Vol. 25, Issue 3
An “Electronic Fluorescent Pictograph” Browser for Exploring and Analyzing Large-Scale Biological Data Sets
journal, August 2007
- Winter, Debbie; Vinegar, Ben; Nahal, Hardeep
- PLoS ONE, Vol. 2, Issue 8
The Mediator Complex Subunits MED14, MED15, and MED16 Are Involved in Defense Signaling Crosstalk in Arabidopsis
journal, December 2016
- Wang, Chenggang; Du, Xuezhu; Mou, Zhonglin
- Frontiers in Plant Science, Vol. 7
Mediator subunit 16 functions in the regulation of iron uptake gene expression in Arabidopsis
journal, May 2014
- Zhang, Yue; Wu, Huilan; Wang, Ning
- New Phytologist, Vol. 203, Issue 3
The human CDK8 subcomplex is a molecular switch that controls Mediator coactivator function
journal, February 2009
- Knuesel, M. T.; Meyer, K. D.; Bernecky, C.
- Genes & Development, Vol. 23, Issue 4
Importance of Mediator complex in the regulation and integration of diverse signaling pathways in plants
journal, September 2015
- Samanta, Subhasis; Thakur, Jitendra K.
- Frontiers in Plant Science, Vol. 6
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
journal, December 2008
- Huang, Da Wei; Sherman, Brad T.; Lempicki, Richard A.
- Nature Protocols, Vol. 4, Issue 1
Plant-plant interactions influence developmental phase transitions, grain productivity and root system architecture in Arabidopsis via auxin and PFT1/MED25 signalling : Auxin and PFT1/MED25 in self plant recognition
journal, July 2017
- Muñoz-Parra, Edith; Pelagio-Flores, Ramón; Raya-González, Javier
- Plant, Cell & Environment, Vol. 40, Issue 9
Inheritance of Trans Chromosomal Methylation patterns from Arabidopsis F1 hybrids
journal, January 2014
- Greaves, Ian K.; Groszmann, Michael; Wang, Aihua
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 5, p. 2017-2022
The MED12-MED13 module of Mediator regulates the timing of embryo patterning in Arabidopsis
journal, December 2009
- Gillmor, C. S.; Park, M. Y.; Smith, M. R.
- Development, Vol. 137, Issue 1
The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum
journal, July 2015
- Wang, Chenggang; Yao, Jin; Du, Xuezhu
- Plant Physiology, Vol. 169, Issue 1
Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo
journal, November 2016
- Jeronimo, Célia; Langelier, Marie-France; Bataille, Alain R.
- Molecular Cell, Vol. 64, Issue 3
Small-sample estimation of negative binomial dispersion, with applications to SAGE data
journal, July 2007
- Robinson, M. D.; Smyth, G. K.
- Biostatistics, Vol. 9, Issue 2
Conserved Alternative Splicing of Arabidopsis Transthyretin-Like Determines Protein Localization and S -Allantoin Synthesis in Peroxisomes
journal, May 2010
- Lamberto, Ilaria; Percudani, Riccardo; Gatti, Rita
- The Plant Cell, Vol. 22, Issue 5
edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
journal, November 2009
- Robinson, M. D.; McCarthy, D. J.; Smyth, G. K.
- Bioinformatics, Vol. 26, Issue 1
HTSeq--a Python framework to work with high-throughput sequencing data
journal, September 2014
- Anders, S.; Pyl, P. T.; Huber, W.
- Bioinformatics, Vol. 31, Issue 2
Plant Mediator complex and its critical functions in transcription regulation: Plant Mediator complex and its function
journal, September 2015
- Yang, Yan; Li, Ling; Qu, Li-Jia
- Journal of Integrative Plant Biology, Vol. 58, Issue 2
The TRAP100 component of the TRAP/Mediator complex is essential in broad transcriptional events and development
journal, July 2002
- Ito, M.
- The EMBO Journal, Vol. 21, Issue 13
The sfr6 Mutation in Arabidopsis Suppresses Low-Temperature Induction of Genes Dependent on the CRT/DRE Sequence Motif
journal, May 1999
- Knight, Heather; Veale, Emma L.; Warren, Gareth J.
- The Plant Cell, Vol. 11, Issue 5
Regulation of flowering time by light quality
journal, June 2003
- Cerdán, Pablo D.; Chory, Joanne
- Nature, Vol. 423, Issue 6942
Differential Use of Signal Peptides and Membrane Domains Is a Common Occurrence in the Protein Output of Transcriptional Units
journal, January 2006
- Davis, Melissa J.; Hanson, Kelly A.; Clark, Francis
- PLoS Genetics, Vol. 2, Issue 4
A conserved Mediator–CDK8 kinase module association regulates Mediator–RNA polymerase II interaction
journal, April 2013
- Tsai, Kuang-Lei; Sato, Shigeo; Tomomori-Sato, Chieri
- Nature Structural & Molecular Biology, Vol. 20, Issue 5
Mediator Complex Regulates Alternative mRNA Processing via the MED23 Subunit
journal, February 2012
- Huang, Yan; Li, Wencheng; Yao, Xiao
- Molecular Cell, Vol. 45, Issue 4
Fiji: an open-source platform for biological-image analysis
journal, June 2012
- Schindelin, Johannes; Arganda-Carreras, Ignacio; Frise, Erwin
- Nature Methods, Vol. 9, Issue 7
Suppression of Mediator is regulated by Cdk8-dependent Grr1 turnover of the Med3 coactivator
journal, February 2014
- Gonzalez, D.; Hamidi, N.; Del Sol, R.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 7
MEDIATOR25 Acts as an Integrative Hub for the Regulation of Jasmonate-Responsive Gene Expression in Arabidopsis
journal, July 2012
- Çevik, Volkan; Kidd, Brendan N.; Zhang, Peijun
- Plant Physiology, Vol. 160, Issue 1
Mediator Med23 deficiency enhances neural differentiation of murine embryonic stem cells through modulating BMP signaling
journal, January 2015
- Zhu, W.; Yao, X.; Liang, Y.
- Development, Vol. 142, Issue 3
Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily
journal, January 2009
- Dixon, David P.; Hawkins, Timothy; Hussey, Patrick J.
- Journal of Experimental Botany, Vol. 60, Issue 4
Mediator protein mutations that selectively abolish activated transcription
journal, January 1999
- Myers, L. C.; Gustafsson, C. M.; Hayashibara, K. C.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 1
SENSITIVE TO FREEZING6 Integrates Cellular and Environmental Inputs to the Plant Circadian Clock
journal, July 2008
- Knight, Heather; Thomson, Adrian J. W.; McWatters, Harriet G.
- Plant Physiology, Vol. 148, Issue 1
The Arabidopsis Mediator Complex Subunits MED16, MED14, and MED2 Regulate Mediator and RNA Polymerase II Recruitment to CBF-Responsive Cold-Regulated Genes
journal, January 2014
- Hemsley, Piers A.; Hurst, Charlotte H.; Kaliyadasa, Ewon
- The Plant Cell, Vol. 26, Issue 1
The Mediator subunit SFR6/MED16 controls defence gene expression mediated by salicylic acid and jasmonate responsive pathways
journal, April 2012
- Wathugala, Deepthi L.; Hemsley, Piers A.; Moffat, Caroline S.
- New Phytologist, Vol. 195, Issue 1
The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II
journal, October 2006
- Elmlund, H.; Baraznenok, V.; Lindahl, M.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 43
Facilitated Assembly of the Preinitiation Complex by Separated Tail and Head/Middle Modules of the Mediator
journal, January 2012
- Galdieri, Luciano; Desai, Parima; Vancura, Ales
- Journal of Molecular Biology, Vol. 415, Issue 3
Mediator Complex Subunits MED2, MED5, MED16, and MED23 Genetically Interact in the Regulation of Phenylpropanoid Biosynthesis
journal, December 2017
- Dolan, Whitney L.; Dilkes, Brian P.; Stout, Jake M.
- The Plant Cell, Vol. 29, Issue 12
The sfr6 mutant of Arabidopsis is defective in transcriptional activation via CBF/DREB1 and DREB2 and shows sensitivity to osmotic stress
journal, May 2003
- Boyce, Joy M.; Knight, Heather; Deyholos, Michael
- The Plant Journal, Vol. 34, Issue 4
Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit
journal, November 2014
- Cevher, Murat A.; Shi, Yi; Li, Dan
- Nature Structural & Molecular Biology, Vol. 21, Issue 12
Identification of SFR6, a key component in cold acclimation acting post-translationally on CBF function: SFR6 and cold acclimation
journal, January 2009
- Knight, Heather; Mugford, Sarah G.; Ülker, Bekir
- The Plant Journal, Vol. 58, Issue 1
The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development
journal, May 2011
- Elfving, N.; Davoine, C.; Benlloch, R.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 20
PFT1, a transcriptional Mediator complex subunit, controls root hair differentiation through reactive oxygen species (ROS) distribution in Arabidopsis
journal, October 2012
- Sundaravelpandian, Kalaipandian; Chandrika, Nulu Naga Prafulla; Schmidt, Wolfgang
- New Phytologist, Vol. 197, Issue 1
Human Mediator Subunit MED26 Functions as a Docking Site for Transcription Elongation Factors
journal, July 2011
- Takahashi, Hidehisa; Parmely, Tari J.; Sato, Shigeo
- Cell, Vol. 146, Issue 1
The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
journal, February 2014
- Yang, Yan; Ou, Bin; Zhang, Jinzhe
- The Plant Journal, Vol. 77, Issue 6
The plant Mediator and its role in noncoding RNA production
journal, April 2011
- Kim, Yun Ju; Chen, Xuemei
- Frontiers in Biology, Vol. 6, Issue 2
FLOWERING LOCUS C Encodes a Novel MADS Domain Protein That Acts as a Repressor of Flowering
journal, May 1999
- Michaels, Scott D.; Amasino, Richard M.
- The Plant Cell, Vol. 11, Issue 5
The Mediator complex and transcription regulation
journal, October 2013
- Poss, Zachary C.; Ebmeier, Christopher C.; Taatjes, Dylan J.
- Critical Reviews in Biochemistry and Molecular Biology, Vol. 48, Issue 6
The Mediator complex: a master coordinator of transcription and cell lineage development
journal, February 2014
- Yin, J. -w.; Wang, G.
- Development, Vol. 141, Issue 5
The Mediator complex: a central integrator of transcription
journal, February 2015
- Allen, Benjamin L.; Taatjes, Dylan J.
- Nature Reviews Molecular Cell Biology, Vol. 16, Issue 3
TSF1 to TSF6, required for silencing the Saccharomyces cerevisiae GAL genes, are global regulatory genes.
journal, July 1993
- Chen, S.; West, R. W.; Ma, J.
- Genetics, Vol. 134, Issue 3
Requirement for RGR1 and SIN4 in RME1-dependent repression in Saccharomyces cerevisiae.
journal, November 1994
- Covitz, P. A.; Song, W.; Mitchell, A. P.
- Genetics, Vol. 138, Issue 3
Resolution of factors required for the initiation of transcription by yeast RNA polymerase II.
journal, July 1990
- Flanagan, P. M.; Kelleher, R. J.; Feaver, W. J.
- Journal of Biological Chemistry, Vol. 265, Issue 19
Regulation of HIS4 expression by the Saccharomyces cerevisiae SIN4 transcriptional regulator.
journal, May 1995
- Jiang, Y. W.; Stillman, D. J.
- Genetics, Vol. 140, Issue 1
Persistence of adoptively transferred T cells with a kinetically engineered IL-2 receptor agonist
journal, January 2020
- Parisi, Giulia; Saco, Justin D.; Salazar, Felix B.
- Nature Communications, Vol. 11, Issue 1
A small library of chalcones induce liver cancer cell death through Akt phosphorylation inhibition
journal, July 2020
- Sahin, Irem Durmaz; Christodoulou, Michael S.; Guzelcan, Ece Akhan
- Scientific Reports, Vol. 10, Issue 1
Two different novel cis -acting elements of erd1 , a clpA homologous Arabidopsis gene function in induction by dehydration stress and dark-induced senescence
journal, January 2003
- Simpson, Sean D.; Nakashima, Kazuo; Narusaka, Yoshihiro
- The Plant Journal, Vol. 33, Issue 2
Athena: a resource for rapid visualization and systematic analysis of Arabidopsis promoter sequences
journal, October 2005
- O'Connor, T. R.; Dyreson, C.; Wyrick, J. J.
- Bioinformatics, Vol. 21, Issue 24
The Role of WRKY Transcription Factors in Plant Immunity: Figure 1.
journal, May 2009
- Pandey, Shree P.; Somssich, Imre E.
- Plant Physiology, Vol. 150, Issue 4
Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) Function as Transcriptional Activators in Abscisic Acid Signaling
journal, December 2002
- Abe, Hiroshi; Urao, Takeshi; Ito, Takuya
- The Plant Cell, Vol. 15, Issue 1
The sfr6 Mutation in Arabidopsis Suppresses Low-Temperature Induction of Genes Dependent on the CRT/DRE Sequence Motif
journal, May 1999
- Knight, Heather; Veale, Emma L.; Warren, Gareth J.
- The Plant Cell, Vol. 11, Issue 5
FLOWERING LOCUS C Encodes a Novel MADS Domain Protein That Acts as a Repressor of Flowering
journal, May 1999
- Michaels, Scott D.; Amasino, Richard M.
- The Plant Cell, Vol. 11, Issue 5
ImageJ2: ImageJ for the next generation of scientific image data
preprint, January 2017
- Rueden, Curtis T.; Schindelin, Johannes; Hiner, Mark C.
- arXiv
The plant Mediator and its role in noncoding RNA production
journal, April 2011
- Kim, Yun Ju; Chen, Xuemei
- Frontiers in Biology, Vol. 6, Issue 2
A novel mediator between activator proteins and the RNA polymerase II transcription apparatus
journal, June 1990
- Kelleher, Raymond J.; Flanagan, Peter M.; Kornberg, Roger D.
- Cell, Vol. 61, Issue 7
A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast
journal, July 1993
- Thompson, Craig M.; Koleske, Anthony J.; Chao, David M.
- Cell, Vol. 73, Issue 7
Human Mediator Subunit MED26 Functions as a Docking Site for Transcription Elongation Factors
journal, July 2011
- Takahashi, Hidehisa; Parmely, Tari J.; Sato, Shigeo
- Cell, Vol. 146, Issue 1
Facilitated Assembly of the Preinitiation Complex by Separated Tail and Head/Middle Modules of the Mediator
journal, January 2012
- Galdieri, Luciano; Desai, Parima; Vancura, Ales
- Journal of Molecular Biology, Vol. 415, Issue 3
Mediator Complex Regulates Alternative mRNA Processing via the MED23 Subunit
journal, February 2012
- Huang, Yan; Li, Wencheng; Yao, Xiao
- Molecular Cell, Vol. 45, Issue 4
Mediator: A key regulator of plant development
journal, November 2016
- Buendía-Monreal, Manuel; Gillmor, C. Stewart
- Developmental Biology, Vol. 419, Issue 1
An Activator Target in the RNA Polymerase II Holoenzyme
journal, May 1998
- Koh, Sang Seok; Ansari, Aseem Z.; Ptashne, Mark
- Molecular Cell, Vol. 1, Issue 6
Regulation of flowering time by light quality
journal, June 2003
- Cerdán, Pablo D.; Chory, Joanne
- Nature, Vol. 423, Issue 6942
Architecture of the RNA polymerase II–Mediator core initiation complex
journal, February 2015
- Plaschka, C.; Larivière, L.; Wenzeck, L.
- Nature, Vol. 518, Issue 7539
A comprehensive fluorescent sensor for spatiotemporal cell cycle analysis in Arabidopsis
journal, September 2020
- Desvoyes, Bénédicte; Arana-Echarri, Ainhoa; Barea, María D.
- Nature Plants, Vol. 6, Issue 11
Targeting redox metabolism: the perfect storm induced by acrylamide poisoning in the brain
journal, January 2020
- Raldúa, Demetrio; Casado, Marta; Prats, Eva
- Scientific Reports, Vol. 10, Issue 1
The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II
journal, October 2006
- Elmlund, H.; Baraznenok, V.; Lindahl, M.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 43
Suppression of Mediator is regulated by Cdk8-dependent Grr1 turnover of the Med3 coactivator
journal, February 2014
- Gonzalez, D.; Hamidi, N.; Del Sol, R.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 7
Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme.
journal, November 1995
- Li, Y.; Bjorklund, S.; Jiang, Y. W.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 24
REF4 and RFR1, Subunits of the Transcriptional Coregulatory Complex Mediator, Are Required for Phenylpropanoid Homeostasis in Arabidopsis
journal, December 2011
- Bonawitz, Nicholas D.; Soltau, Whitney L.; Blatchley, Michael R.
- Journal of Biological Chemistry, Vol. 287, Issue 8
The Structural and Functional Role of Med5 in the Yeast Mediator Tail Module
journal, October 2005
- Béve, Jenny; Hu, Guo-Zhen; Myers, Lawrence C.
- Journal of Biological Chemistry, Vol. 280, Issue 50
TopHat: discovering splice junctions with RNA-Seq
journal, March 2009
- Trapnell, Cole; Pachter, Lior; Salzberg, Steven L.
- Bioinformatics, Vol. 25, Issue 9
Small-sample estimation of negative binomial dispersion, with applications to SAGE data
journal, July 2007
- Robinson, M. D.; Smyth, G. K.
- Biostatistics, Vol. 9, Issue 2
The TRAP100 component of the TRAP/Mediator complex is essential in broad transcriptional events and development
journal, July 2002
- Ito, M.
- The EMBO Journal, Vol. 21, Issue 13
Requirement for RGR1 and SIN4 in RME1-dependent repression in Saccharomyces cerevisiae.
journal, November 1994
- Covitz, P. A.; Song, W.; Mitchell, A. P.
- Genetics, Vol. 138, Issue 3
Regulation of HIS4 expression by the Saccharomyces cerevisiae SIN4 transcriptional regulator.
journal, May 1995
- Jiang, Y. W.; Stillman, D. J.
- Genetics, Vol. 140, Issue 1
Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily
journal, January 2009
- Dixon, David P.; Hawkins, Timothy; Hussey, Patrick J.
- Journal of Experimental Botany, Vol. 60, Issue 4
Comparative genomics supports a deep evolutionary origin for the large, four-module transcriptional mediator complex
journal, May 2008
- Bourbon, Henri-Marc
- Nucleic Acids Research, Vol. 36, Issue 12
Conservation and Divergence of Mediator Structure and Function: Insights from Plants
journal, November 2016
- Dolan, Whitney L.; Chapple, Clint
- Plant and Cell Physiology
The human CDK8 subcomplex is a molecular switch that controls Mediator coactivator function
journal, February 2009
- Knuesel, M. T.; Meyer, K. D.; Bernecky, C.
- Genes & Development, Vol. 23, Issue 4
PHYTOCHROME AND FLOWERING TIME1/MEDIATOR25 Regulates Lateral Root Formation via Auxin Signaling in Arabidopsis
journal, April 2014
- Raya-González, Javier; Ortiz-Castro, Randy; Ruíz-Herrera, León Francisco
- Plant Physiology, Vol. 165, Issue 2
The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum
journal, July 2015
- Wang, Chenggang; Yao, Jin; Du, Xuezhu
- Plant Physiology, Vol. 169, Issue 1
Conserved Alternative Splicing of Arabidopsis Transthyretin-Like Determines Protein Localization and S -Allantoin Synthesis in Peroxisomes
journal, May 2010
- Lamberto, Ilaria; Percudani, Riccardo; Gatti, Rita
- The Plant Cell, Vol. 22, Issue 5
The sfr6 Mutation in Arabidopsis Suppresses Low-Temperature Induction of Genes Dependent on the CRT/DRE Sequence Motif
journal, May 1999
- Knight, Heather; Veale, Emma L.; Warren, Gareth J.
- The Plant Cell, Vol. 11, Issue 5
FLOWERING LOCUS C Encodes a Novel MADS Domain Protein That Acts as a Repressor of Flowering
journal, May 1999
- Michaels, Scott D.; Amasino, Richard M.
- The Plant Cell, Vol. 11, Issue 5
The Arabidopsis Mediator Complex Subunit16 Positively Regulates Salicylate-Mediated Systemic Acquired Resistance and Jasmonate/Ethylene-Induced Defense Pathways
journal, October 2012
- Zhang, Xudong; Wang, Chenggang; Zhang, Yanping
- The Plant Cell, Vol. 24, Issue 10
Complexity of the Alternative Splicing Landscape in Plants
journal, October 2013
- Reddy, Anireddy S. N.; Marquez, Yamile; Kalyna, Maria
- The Plant Cell, Vol. 25, Issue 10
The Arabidopsis Mediator Complex Subunits MED16, MED14, and MED2 Regulate Mediator and RNA Polymerase II Recruitment to CBF-Responsive Cold-Regulated Genes
journal, January 2014
- Hemsley, Piers A.; Hurst, Charlotte H.; Kaliyadasa, Ewon
- The Plant Cell, Vol. 26, Issue 1
Mediator Complex Subunits MED2, MED5, MED16, and MED23 Genetically Interact in the Regulation of Phenylpropanoid Biosynthesis
journal, December 2017
- Dolan, Whitney L.; Dilkes, Brian P.; Stout, Jake M.
- The Plant Cell, Vol. 29, Issue 12
The Mediator subunit SFR6/MED16 controls defence gene expression mediated by salicylic acid and jasmonate responsive pathways
journal, April 2012
- Wathugala, Deepthi L.; Hemsley, Piers A.; Moffat, Caroline S.
- New Phytologist, Vol. 195, Issue 1
Mediator subunit 16 functions in the regulation of iron uptake gene expression in Arabidopsis
journal, May 2014
- Zhang, Yue; Wu, Huilan; Wang, Ning
- New Phytologist, Vol. 203, Issue 3
The Arabidopsis Mediator complex subunits MED14/SWP and MED16/SFR6/IEN1 differentially regulate defense gene expression in plant immune responses
journal, May 2013
- Zhang, Xudong; Yao, Jin; Zhang, Yanping
- The Plant Journal, Vol. 75, Issue 3
The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
journal, February 2014
- Yang, Yan; Ou, Bin; Zhang, Jinzhe
- The Plant Journal, Vol. 77, Issue 6
In Vivo Requirement of Activator-Specific Binding Targets of Mediator
journal, December 2000
- Park, J. M.; Kim, H. -S.; Han, S. J.
- Molecular and Cellular Biology, Vol. 20, Issue 23
Activator-Independent Functions of the Yeast Mediator Sin4 Complex in Preinitiation Complex Formation and Transcription Reinitiation
journal, January 2003
- Reeves, W. M.; Hahn, S.
- Molecular and Cellular Biology, Vol. 23, Issue 1
A Triad of Subunits from the Gal11/Tail Domain of Srb Mediator Is an In Vivo Target of Transcriptional Activator Gcn4p
journal, July 2004
- Zhang, F.; Sumibcay, L.; Hinnebusch, A. G.
- Molecular and Cellular Biology, Vol. 24, Issue 15
The Mediator complex subunits MED25/PFT1 and MED8 are required for transcriptional responses to changes in cell wall arabinose composition and glucose treatment in Arabidopsis thaliana
journal, September 2015
- Seguela-Arnaud, Mathilde; Smith, Caroline; Uribe, Marcos Castellanos
- BMC Plant Biology, Vol. 15, Issue 1
The MED12-MED13 module of Mediator regulates the timing of embryo patterning in Arabidopsis
journal, December 2009
- Gillmor, C. S.; Park, M. Y.; Smith, M. R.
- Development, Vol. 137, Issue 1
Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
journal, September 2011
- Xu, R.; Li, Y.
- Development, Vol. 138, Issue 20
Differential Use of Signal Peptides and Membrane Domains Is a Common Occurrence in the Protein Output of Transcriptional Units
journal, January 2006
- Davis, Melissa J.; Hanson, Kelly A.; Clark, Francis
- PLoS Genetics, Vol. 2, Issue 4
An “Electronic Fluorescent Pictograph” Browser for Exploring and Analyzing Large-Scale Biological Data Sets
journal, August 2007
- Winter, Debbie; Vinegar, Ben; Nahal, Hardeep
- PLoS ONE, Vol. 2, Issue 8
The M ediator subunit MED 23 couples H2B mono‐ubiquitination to transcriptional control and cell fate determination
journal, September 2015
- Yao, Xiao; Tang, Zhanyun; Fu, Xing
- The EMBO Journal, Vol. 34, Issue 23
Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures.
journalarticle, January 2018
- Sozen, Berna; Amadei, Gianluca; Cox, Andy
- Springer Science and Business Media LLC
Role of Arabidopsis MYC and MYB Homologs in Drought- and Abscisic Acid-Regulated Gene Expression
journal, October 1997
- Abe, Hiroshi; Yamaguchi-Shinozaki, Kazuko; Urao, Takeshi
- The Plant Cell, Vol. 9, Issue 10
Importance of Mediator complex in the regulation and integration of diverse signaling pathways in plants
journal, September 2015
- Samanta, Subhasis; Thakur, Jitendra K.
- Frontiers in Plant Science, Vol. 6
The Mediator Complex Subunits MED14, MED15, and MED16 Are Involved in Defense Signaling Crosstalk in Arabidopsis
journal, December 2016
- Wang, Chenggang; Du, Xuezhu; Mou, Zhonglin
- Frontiers in Plant Science, Vol. 7
Plant mediator
journal, June 2010
- Kidd, Brendan N.; Aitken, Elizabeth A.; Schenk, Peer M.
- Plant Signaling & Behavior, Vol. 5, Issue 6
Mediator MED23 regulates basal transcription in vivo via an interaction with P-TEFb
journal, January 2013
- Wang, Wei; Yao, Xiao; Huang, Yan
- Transcription, Vol. 4, Issue 1
Molecular architecture of the yeast Mediator complex
journal, September 2015
- Robinson, Philip J.; Trnka, Michael J.; Pellarin, Riccardo
- eLife, Vol. 4
Molecular Architecture Of The Yeast Mediator Complex
dataset, January 2015
- Robinson, Philip J.; Trnka, Michael J.; Pellarin, Riccardo
- Zenodo
Additional file 2: of The Mediator complex subunits MED25/PFT1 and MED8 are required for transcriptional responses to changes in cell wall arabinose composition and glucose treatment in Arabidopsis thaliana
dataset, January 2015
- Seguela-Arnaud, Mathilde; Smith, Caroline; Uribe, Marcos
- Figshare
Works referencing / citing this record:
NeVOmics: An Enrichment Tool for Gene Ontology and Functional Network Analysis and Visualization of Data from OMICs Technologies
journal, November 2018
- Zúñiga-León, Eduardo; Carrasco-Navarro, Ulises; Fierro, Francisco
- Genes, Vol. 9, Issue 12