Genomic Comparison of Two Family-Level Groups of the Uncultivated NAG1 Archaeal Lineage from Chemically and Geographically Disparate Hot Springs
Abstract
Recent progress based on single-cell genomics and metagenomic investigations of archaea in a variety of extreme environments has led to significant advances in our understanding of the diversity, evolution, and metabolic potential of archaea, yet the vast majority of archaeal diversity remains undersampled. In this work, we coordinated single-cell genomics with metagenomics in order to construct a near-complete genome from a deeply branching uncultivated archaeal lineage sampled from Great Boiling Spring (GBS) in the U.S. Great Basin, Nevada. This taxon is distantly related (distinct families) to an archaeal genome, designated “Novel Archaeal Group 1” (NAG1), which was extracted from a metagenome recovered from an acidic iron spring in Yellowstone National Park (YNP). We compared the metabolic predictions of the NAG1 lineage to better understand how these archaea could inhabit such chemically distinct environments. Similar to the NAG1 population previously studied in YNP, the NAG1 population from GBS is predicted to utilize proteins as a primary carbon source, ferment simple carbon sources, and use oxygen as a terminal electron acceptor under oxic conditions. However, GBS NAG1 populations contained distinct genes involved in central carbon metabolism and electron transfer, including nitrite reductase, which could confer the ability to reduce nitrite under anaerobicmore »
- Authors:
-
- Northern Illinois Univ., DeKalb, IL (United States); Bigelow Lab. for Ocean Sciences, East Boothbay, ME (United States)
- California State Univ. (CalState), San Bernardino, CA (United States); Univ. of Nevada, Las Vegas, NV (United States)
- Univ. of Nevada, Las Vegas, NV (United States); Université Paris-Saclay, Jouy-en-Josas (France). Institut National de la Recherche Agronomique (INRA)
- Univ. of Nevada, Las Vegas, NV (United States)
- Northern Illinois Univ., DeKalb, IL (United States)
- Bigelow Lab. for Ocean Sciences, East Boothbay, ME (United States)
- Publication Date:
- Research Org.:
- Nevada System of Higher Education (NSHE), Las Vegas, NV (United States); University of California, Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE); USDOE Office of Science (SC)
- OSTI Identifier:
- 1510510
- Grant/Contract Number:
- EE0000716; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 8; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; extreme microbiology; microbial ecology; uncultivated archaea; NAG1 lineage; Great Boiling Spring
Citation Formats
Becraft, Eric D., Dodsworth, Jeremy A., Murugapiran, Senthil K., Thomas, Scott C., Ohlsson, J. Ingemar, Stepanauskas, Ramunas, Hedlund, Brian P., and Swingley, Wesley D. Genomic Comparison of Two Family-Level Groups of the Uncultivated NAG1 Archaeal Lineage from Chemically and Geographically Disparate Hot Springs. United States: N. p., 2017.
Web. doi:10.3389/fmicb.2017.02082.
Becraft, Eric D., Dodsworth, Jeremy A., Murugapiran, Senthil K., Thomas, Scott C., Ohlsson, J. Ingemar, Stepanauskas, Ramunas, Hedlund, Brian P., & Swingley, Wesley D. Genomic Comparison of Two Family-Level Groups of the Uncultivated NAG1 Archaeal Lineage from Chemically and Geographically Disparate Hot Springs. United States. https://doi.org/10.3389/fmicb.2017.02082
Becraft, Eric D., Dodsworth, Jeremy A., Murugapiran, Senthil K., Thomas, Scott C., Ohlsson, J. Ingemar, Stepanauskas, Ramunas, Hedlund, Brian P., and Swingley, Wesley D. Tue .
"Genomic Comparison of Two Family-Level Groups of the Uncultivated NAG1 Archaeal Lineage from Chemically and Geographically Disparate Hot Springs". United States. https://doi.org/10.3389/fmicb.2017.02082. https://www.osti.gov/servlets/purl/1510510.
@article{osti_1510510,
title = {Genomic Comparison of Two Family-Level Groups of the Uncultivated NAG1 Archaeal Lineage from Chemically and Geographically Disparate Hot Springs},
author = {Becraft, Eric D. and Dodsworth, Jeremy A. and Murugapiran, Senthil K. and Thomas, Scott C. and Ohlsson, J. Ingemar and Stepanauskas, Ramunas and Hedlund, Brian P. and Swingley, Wesley D.},
abstractNote = {Recent progress based on single-cell genomics and metagenomic investigations of archaea in a variety of extreme environments has led to significant advances in our understanding of the diversity, evolution, and metabolic potential of archaea, yet the vast majority of archaeal diversity remains undersampled. In this work, we coordinated single-cell genomics with metagenomics in order to construct a near-complete genome from a deeply branching uncultivated archaeal lineage sampled from Great Boiling Spring (GBS) in the U.S. Great Basin, Nevada. This taxon is distantly related (distinct families) to an archaeal genome, designated “Novel Archaeal Group 1” (NAG1), which was extracted from a metagenome recovered from an acidic iron spring in Yellowstone National Park (YNP). We compared the metabolic predictions of the NAG1 lineage to better understand how these archaea could inhabit such chemically distinct environments. Similar to the NAG1 population previously studied in YNP, the NAG1 population from GBS is predicted to utilize proteins as a primary carbon source, ferment simple carbon sources, and use oxygen as a terminal electron acceptor under oxic conditions. However, GBS NAG1 populations contained distinct genes involved in central carbon metabolism and electron transfer, including nitrite reductase, which could confer the ability to reduce nitrite under anaerobic conditions. Despite inhabiting chemically distinct environments with large variations in pH, GBS NAG1 populations shared many core genomic and metabolic features with the archaeon identified from YNP, yet were able to carve out a distinct niche at GBS.},
doi = {10.3389/fmicb.2017.02082},
journal = {Frontiers in Microbiology},
number = ,
volume = 8,
place = {United States},
year = {Tue Oct 31 00:00:00 EDT 2017},
month = {Tue Oct 31 00:00:00 EDT 2017}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 2 works
Citation information provided by
Web of Science
Web of Science
Figures / Tables:
Table 1: NAG1 statistics for the Great Boiling Spring (GBS) single assembled genome (SAG) co-assembly, the GBS MLP metagenome assembly, and the Yellowstone National Park (YNP) metagenome assembly (Kozubal et al., 2013).
All figures and tables
(3 total)
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Detection and analysis of elusive members of a novel and diverse archaeal community within a thermal spring streamer consortium
journal, December 2014
- Colman, Daniel R.; Thomas, Raquela; Maas, Kendra R.
- Extremophiles, Vol. 19, Issue 2
Asgard archaea illuminate the origin of eukaryotic cellular complexity
journal, January 2017
- Zaremba-Niedzwiedzka, Katarzyna; Caceres, Eva F.; Saw, Jimmy H.
- Nature, Vol. 541, Issue 7637
Active-site remodelling in the bifunctional fructose-1,6-bisphosphate aldolase/phosphatase
journal, October 2011
- Du, Juan; Say, Rafael F.; Lü, Wei
- Nature, Vol. 478, Issue 7370
Carbohydrate Metabolism in Archaea: Current Insights into Unusual Enzymes and Pathways and Their Regulation
journal, March 2014
- Brasen, C.; Esser, D.; Rauch, B.
- Microbiology and Molecular Biology Reviews, Vol. 78, Issue 1
The Binning of Metagenomic Contigs for Microbial Physiology of Mixed Cultures
journal, January 2012
- Strous, Marc; Kraft, Beate; Bisdorf, Regina
- Frontiers in Microbiology, Vol. 3
The YNP metagenome project: environmental parameters responsible for microbial distribution in the Yellowstone geothermal ecosystem
journal, January 2013
- Inskeep, William
- Frontiers in Microbiology, Vol. 4
Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea
journal, August 2017
- Bowers, Robert M.; Kyrpides, Nikos C.; Stepanauskas, Ramunas
- Nature Biotechnology, Vol. 35, Issue 8
Geoarchaeota: a new candidate phylum in the Archaea from high-temperature acidic iron mats in Yellowstone National Park
journal, November 2012
- Kozubal, Mark A.; Romine, Margaret; Jennings, Ryan deM
- The ISME Journal, Vol. 7, Issue 3
CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats
journal, May 2007
- Grissa, I.; Vergnaud, G.; Pourcel, C.
- Nucleic Acids Research, Vol. 35, Issue Web Server
Single-Cell-Genomics-Facilitated Read Binning of Candidate Phylum EM19 Genomes from Geothermal Spring Metagenomes
journal, December 2015
- Becraft, Eric D.; Dodsworth, Jeremy A.; Murugapiran, Senthil K.
- Applied and Environmental Microbiology, Vol. 82, Issue 4
A Comprehensive Census of Microbial Diversity in Hot Springs of Tengchong, Yunnan Province China Using 16S rRNA Gene Pyrosequencing
journal, January 2013
- Hou, Weiguo; Wang, Shang; Dong, Hailiang
- PLoS ONE, Vol. 8, Issue 1
Evaluation of methods for extraction of bacteria from soil
journal, February 1995
- Lindahl, Viggo; Bakken, Lars R.
- FEMS Microbiology Ecology, Vol. 16, Issue 2
BlastKOALA and GhostKOALA: KEGG Tools for Functional Characterization of Genome and Metagenome Sequences
journal, February 2016
- Kanehisa, Minoru; Sato, Yoko; Morishima, Kanae
- Journal of Molecular Biology, Vol. 428, Issue 4
Microbial diversity and potential for arsenic and iron biogeochemical cycling at an arsenic rich, shallow-sea hydrothermal vent (Tutum Bay, Papua New Guinea)
journal, June 2013
- Meyer-Dombard, D'Arcy R.; Amend, Jan P.; Osburn, Magdalena R.
- Chemical Geology, Vol. 348
Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences
journal, August 2014
- Yarza, Pablo; Yilmaz, Pelin; Pruesse, Elmar
- Nature Reviews Microbiology, Vol. 12, Issue 9
Autotrophic carbon fixation in archaea
journal, May 2010
- Berg, Ivan A.; Kockelkorn, Daniel; Ramos-Vera, W. Hugo
- Nature Reviews Microbiology, Vol. 8, Issue 6, p. 447-460
Distribution, diversity and ecology of aerobic CO-oxidizing bacteria
journal, February 2007
- King, Gary M.; Weber, Carolyn F.
- Nature Reviews Microbiology, Vol. 5, Issue 2
Ammonia oxidation, denitrification and dissimilatory nitrate reduction to ammonium in two US Great Basin hot springs with abundant ammonia-oxidizing archaea: N-cycling in Great Basin hot springs
journal, June 2011
- Dodsworth, Jeremy A.; Hungate, Bruce A.; Hedlund, Brian P.
- Environmental Microbiology, Vol. 13, Issue 8
Potential role of Thermus thermophilus and T. oshimai in high rates of nitrous oxide (N2O) production in ∼80 °C hot springs in the US Great Basin: N2O production by Thermus thermophilus and T. oshimai
journal, September 2011
- Hedlund, B. P.; Mcdonald, A. I.; Lam, J.
- Geobiology, Vol. 9, Issue 6
CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes
journal, May 2015
- Parks, Donovan H.; Imelfort, Michael; Skennerton, Connor T.
- Genome Research, Vol. 25, Issue 7
Alkaline pH homeostasis in bacteria: New insights
journal, November 2005
- Padan, Etana; Bibi, Eitan; Ito, Masahiro
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1717, Issue 2
The RAST Server: Rapid Annotations using Subsystems Technology
journal, January 2008
- Aziz, Ramy K.; Bartels, Daniela; Best, Aaron A.
- BMC Genomics, Vol. 9, Issue 1, Article No. 75
Hydrothermal ecotones and streamer biofilm communities in the Lower Geyser Basin, Yellowstone National Park: Hydrothermal ecotones and biofilms in Yellowstone
journal, March 2011
- Meyer-Dombard, D'Arcy R.; Swingley, Wesley; Raymond, Jason
- Environmental Microbiology, Vol. 13, Issue 8
Genomic Expansion of Domain Archaea Highlights Roles for Organisms from New Phyla in Anaerobic Carbon Cycling
journal, March 2015
- Castelle, Cindy J.; Wrighton, Kelly C.; Thomas, Brian C.
- Current Biology, Vol. 25, Issue 6
The hybrid-cluster protein (‘prismane protein’) from Escherichia coli: Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxi
journal, February 2000
- van den Berg, Willy A. M.; Hagen, Wilfred R.; van Dongen, Walter M. A. M.
- European Journal of Biochemistry, Vol. 267, Issue 3
Sediment microbial communities in Great Boiling Spring are controlled by temperature and distinct from water communities
journal, December 2012
- Cole, Jessica K.; Peacock, Joseph P.; Dodsworth, Jeremy A.
- The ISME Journal, Vol. 7, Issue 4
The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
journal, November 2012
- Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin
- Nucleic Acids Research, Vol. 41, Issue D1
Transaldolase of Methanocaldococcus jannaschii
journal, January 2004
- Soderberg, Tim; Alver, Robert C.
- Archaea, Vol. 1, Issue 4
Microbiology and geochemistry of great boiling and mud hot springs in the United States Great Basin
journal, February 2009
- Costa, Kyle C.; Navarro, Jason B.; Shock, Everett L.
- Extremophiles, Vol. 13, Issue 3
SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
journal, May 2012
- Bankevich, Anton; Nurk, Sergey; Antipov, Dmitry
- Journal of Computational Biology, Vol. 19, Issue 5
Thermoflexus hugenholtzii gen. nov., sp. nov., a thermophilic, microaerophilic, filamentous bacterium representing a novel class in the Chloroflexi, Thermoflexia classis nov., and description of Thermoflexaceae fam. nov. and Thermoflexales ord. nov.
journal, March 2014
- Dodsworth, J. A.; Gevorkian, J.; Despujos, F.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 64, Issue Pt 6
The respiratory complex I of bacteria, archaea and eukarya and its module common with membrane-bound multisubunit hydrogenases
journal, August 2000
- Friedrich, Thorsten; Scheide, Dierk
- FEBS Letters, Vol. 479, Issue 1-2
Evidence for strong selective constraint acting on the nucleotide composition of 16S ribosomal RNA genes
journal, June 2002
- Wang, H. -c.
- Nucleic Acids Research, Vol. 30, Issue 11
Single-cell and metagenomic analyses indicate a fermentative and saccharolytic lifestyle for members of the OP9 lineage
journal, May 2013
- Dodsworth, Jeremy A.; Blainey, Paul C.; Murugapiran, Senthil K.
- Nature Communications, Vol. 4, Issue 1
Phylogenetic and Functional Analysis of Metagenome Sequence from High-Temperature Archaeal Habitats Demonstrate Linkages between Metabolic Potential and Geochemistry
journal, January 2013
- Inskeep, William P.; Jay, Zackary J.; Herrgard, Markus J.
- Frontiers in Microbiology, Vol. 4
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
Accurate phylogenetic classification of variable-length DNA fragments
journal, December 2006
- McHardy, Alice Carolyn; Martín, Héctor García; Tsirigos, Aristotelis
- Nature Methods, Vol. 4, Issue 1
The growing tree of Archaea: new perspectives on their diversity, evolution and ecology
journal, August 2017
- Adam, Panagiotis S.; Borrel, Guillaume; Brochier-Armanet, Céline
- The ISME Journal, Vol. 11, Issue 11
A ubiquitous thermoacidophilic archaeon from deep-sea hydrothermal vents
journal, July 2006
- Reysenbach, Anna-Louise; Liu, Yitai; Banta, Amy B.
- Nature, Vol. 442, Issue 7101
Microbial Iron Cycling in Acidic Geothermal Springs of Yellowstone National Park: Integrating Molecular Surveys, Geochemical Processes, and Isolation of Novel Fe-Active Microorganisms
journal, January 2012
- Kozubal, Mark A.; Macur, Richard E.; Jay, Zackary J.
- Frontiers in Microbiology, Vol. 3
Archaea in Symbioses
journal, January 2012
- Wrede, Christoph; Dreier, Anne; Kokoschka, Sebastian
- Archaea, Vol. 2012
Thousands of microbial genomes shed light on interconnected biogeochemical processes in an aquifer system
journal, October 2016
- Anantharaman, Karthik; Brown, Christopher T.; Hug, Laura A.
- Nature Communications, Vol. 7, Issue 1
Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum
journal, January 2002
- Fitz-Gibbon, S. T.; Ladner, H.; Kim, U. -J.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 2
Insights into the phylogeny and coding potential of microbial dark matter
journal, July 2013
- Rinke, Christian; Schwientek, Patrick; Sczyrba, Alexander
- Nature, Vol. 499, Issue 7459
‘Geoarchaeote NAG1’ is a deeply rooting lineage of the archaeal order Thermoproteales rather than a new phylum
journal, February 2014
- Guy, Lionel; Spang, Anja; Saw, Jimmy H.
- The ISME Journal, Vol. 8, Issue 7
Impact of single-cell genomics and metagenomics on the emerging view of extremophile “microbial dark matter”
journal, August 2014
- Hedlund, Brian P.; Dodsworth, Jeremy A.; Murugapiran, Senthil K.
- Extremophiles, Vol. 18, Issue 5
MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0
journal, October 2013
- Tamura, Koichiro; Stecher, Glen; Peterson, Daniel
- Molecular Biology and Evolution, Vol. 30, Issue 12
Hydrogenases and H+-Reduction in Primary Energy Conservation
book,
- Vignais, Paulette M.
- Bioenergetics
Assembly and Succession of Iron Oxide Microbial Mat Communities in Acidic Geothermal Springs
journal, February 2016
- Beam, Jacob P.; Bernstein, Hans C.; Jay, Zackary J.
- Frontiers in Microbiology, Vol. 7
DNA–DNA hybridization values and their relationship to whole-genome sequence similarities
journal, January 2007
- Klappenbach, Joel A.; Goris, Johan; Vandamme, Peter
- International Journal of Systematic and Evolutionary Microbiology, Vol. 57, Issue 1
CDD/SPARCLE: functional classification of proteins via subfamily domain architectures
journal, November 2016
- Marchler-Bauer, Aron; Bo, Yu; Han, Lianyi
- Nucleic Acids Research, Vol. 45, Issue D1
Impact of single-cell genomics and metagenomics on the emerging view of extremophile “microbial dark matter”
journal, August 2014
- Hedlund, Brian P.; Dodsworth, Jeremy A.; Murugapiran, Senthil K.
- Extremophiles, Vol. 18, Issue 5
Alkaline pH homeostasis in bacteria: New insights
journal, November 2005
- Padan, Etana; Bibi, Eitan; Ito, Masahiro
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1717, Issue 2
Genomic Expansion of Domain Archaea Highlights Roles for Organisms from New Phyla in Anaerobic Carbon Cycling
journal, March 2015
- Castelle, Cindy J.; Wrighton, Kelly C.; Thomas, Brian C.
- Current Biology, Vol. 25, Issue 6
The growing tree of Archaea: new perspectives on their diversity, evolution and ecology
journal, August 2017
- Adam, Panagiotis S.; Borrel, Guillaume; Brochier-Armanet, Céline
- The ISME Journal, Vol. 11, Issue 11
Asgard archaea illuminate the origin of eukaryotic cellular complexity
journal, January 2017
- Zaremba-Niedzwiedzka, Katarzyna; Caceres, Eva F.; Saw, Jimmy H.
- Nature, Vol. 541, Issue 7637
Erratum: Corrigendum: Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea
journal, July 2018
- Bowers, Robert M.; Kyrpides, Nikos C.; Stepanauskas, Ramunas
- Nature Biotechnology, Vol. 36, Issue 7
Single-cell and metagenomic analyses indicate a fermentative and saccharolytic lifestyle for members of the OP9 lineage
journal, May 2013
- Dodsworth, Jeremy A.; Blainey, Paul C.; Murugapiran, Senthil K.
- Nature Communications, Vol. 4, Issue 1
Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum
journal, January 2002
- Fitz-Gibbon, S. T.; Ladner, H.; Kim, U. -J.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 2
CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats
journal, May 2007
- Grissa, I.; Vergnaud, G.; Pourcel, C.
- Nucleic Acids Research, Vol. 35, Issue Web Server
Ammonia oxidation, denitrification and dissimilatory nitrate reduction to ammonium in two US Great Basin hot springs with abundant ammonia-oxidizing archaea: N-cycling in Great Basin hot springs
journal, June 2011
- Dodsworth, Jeremy A.; Hungate, Bruce A.; Hedlund, Brian P.
- Environmental Microbiology, Vol. 13, Issue 8
Another Unusual Type of Citric Acid Cycle Enzyme in Helicobacter pylori: the Malate:Quinone Oxidoreductase
journal, June 2000
- Kather, B.; Stingl, K.; van der Rest, M. E.
- Journal of Bacteriology, Vol. 182, Issue 11
Cytochrome bd Oxidase, Oxidative Stress, and Dioxygen Tolerance of the Strictly Anaerobic Bacterium Moorella thermoacetica
journal, March 2005
- Das, A.; Silaghi-Dumitrescu, R.; Ljungdahl, L. G.
- Journal of Bacteriology, Vol. 187, Issue 6
Identification and Characterization of an ATP-Dependent Hexokinase with Broad Substrate Specificity from the Hyperthermophilic Archaeon Sulfolobus tokodaii
journal, February 2006
- Nishimasu, H.; Fushinobu, S.; Shoun, H.
- Journal of Bacteriology, Vol. 188, Issue 5
Transaldolase of Methanocaldococcus jannaschii
journal, January 2004
- Soderberg, Tim; Alver, Robert C.
- Archaea, Vol. 1, Issue 4
The RAST Server: Rapid Annotations using Subsystems Technology
journal, January 2008
- Aziz, Ramy K.; Bartels, Daniela; Best, Aaron A.
- BMC Genomics, Vol. 9, Issue 1, Article No. 75
Works referencing / citing this record:
Position-Specific Metabolic Probing and Metagenomics of Microbial Communities Reveal Conserved Central Carbon Metabolic Network Activities at High Temperatures
journal, July 2019
- Thomas, Scott C.; Tamadonfar, Kevin O.; Seymour, Cale O.
- Frontiers in Microbiology, Vol. 10
Position-Specific Metabolic Probing and Metagenomics of Microbial Communities Reveal Conserved Central Carbon Metabolic Network Activities at High Temperatures
journal, July 2019
- Thomas, Scott C.; Tamadonfar, Kevin O.; Seymour, Cale O.
- Frontiers in Microbiology, Vol. 10
Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.