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Title: The Role of Mass Spectrometry in Structural Studies of Flavin-Based Electron Bifurcating Enzymes

Abstract

For decades, biologists and biochemists have taken advantage of atomic resolution structural models of proteins from X-ray crystallography, nuclear magnetic resonance spectroscopy, and more recently cryo-electron microscopy. However, not all proteins relent to structural analyses using these approaches, and as the depth of knowledge increases, additional data elucidating a mechanistic understanding of protein function is desired. Flavin-based electron bifurcating enzymes, which are responsible for producing high energy compounds through the simultaneous endergonic and exergonic reduction of two intercellular electron carriers (i.e., NAD+ and ferredoxin) are one class of proteins that have challenged structural biologists and in which there is great interest to understand the mechanism behind electron gating. A limited number of X-ray crystallography projects have been successful; however, it is clear that to understand how these enzymes function, techniques that can reveal detailed in solution information about protein structure, dynamics, and interactions involved in the bifurcating reaction are needed. In this review, we cover a general set of mass spectrometry-based techniques that, combined with protein modeling, are capable of providing information on both protein structure and dynamics. Techniques discussed include surface labeling, covalent cross-linking, native mass spectrometry, and hydrogen/deuterium exchange. We cover how biophysical data can be used tomore » validate computationally generated protein models and develop mechanistic explanations for regulation and performance of enzymes and protein complexes. Our focus will be on flavin-based electron bifurcating enzymes, but the broad applicability of the techniques will be showcased.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
  1. Montana State Univ., Bozeman, MT (United States). Dept. of Chemistry and Biochemistry
Publication Date:
Research Org.:
Montana State Univ., Bozeman, MT (United States).
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1510453
Grant/Contract Number:  
SC0012518
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 9; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; chemical cross-linking; hydrogen deuterium exchange; protein labeling; native mass spectrometry; electron bifurcation; protein structure; protein-modeling; mass spectrometry

Citation Formats

Tokmina-Lukaszewska, Monika, Patterson, Angela, Berry, Luke, Scott, Liam, Balasubramanian, Narayanaganesh, and Bothner, Brian. The Role of Mass Spectrometry in Structural Studies of Flavin-Based Electron Bifurcating Enzymes. United States: N. p., 2018. Web. doi:10.3389/fmicb.2018.01397.
Tokmina-Lukaszewska, Monika, Patterson, Angela, Berry, Luke, Scott, Liam, Balasubramanian, Narayanaganesh, & Bothner, Brian. The Role of Mass Spectrometry in Structural Studies of Flavin-Based Electron Bifurcating Enzymes. United States. doi:10.3389/fmicb.2018.01397.
Tokmina-Lukaszewska, Monika, Patterson, Angela, Berry, Luke, Scott, Liam, Balasubramanian, Narayanaganesh, and Bothner, Brian. Thu . "The Role of Mass Spectrometry in Structural Studies of Flavin-Based Electron Bifurcating Enzymes". United States. doi:10.3389/fmicb.2018.01397. https://www.osti.gov/servlets/purl/1510453.
@article{osti_1510453,
title = {The Role of Mass Spectrometry in Structural Studies of Flavin-Based Electron Bifurcating Enzymes},
author = {Tokmina-Lukaszewska, Monika and Patterson, Angela and Berry, Luke and Scott, Liam and Balasubramanian, Narayanaganesh and Bothner, Brian},
abstractNote = {For decades, biologists and biochemists have taken advantage of atomic resolution structural models of proteins from X-ray crystallography, nuclear magnetic resonance spectroscopy, and more recently cryo-electron microscopy. However, not all proteins relent to structural analyses using these approaches, and as the depth of knowledge increases, additional data elucidating a mechanistic understanding of protein function is desired. Flavin-based electron bifurcating enzymes, which are responsible for producing high energy compounds through the simultaneous endergonic and exergonic reduction of two intercellular electron carriers (i.e., NAD+ and ferredoxin) are one class of proteins that have challenged structural biologists and in which there is great interest to understand the mechanism behind electron gating. A limited number of X-ray crystallography projects have been successful; however, it is clear that to understand how these enzymes function, techniques that can reveal detailed in solution information about protein structure, dynamics, and interactions involved in the bifurcating reaction are needed. In this review, we cover a general set of mass spectrometry-based techniques that, combined with protein modeling, are capable of providing information on both protein structure and dynamics. Techniques discussed include surface labeling, covalent cross-linking, native mass spectrometry, and hydrogen/deuterium exchange. We cover how biophysical data can be used to validate computationally generated protein models and develop mechanistic explanations for regulation and performance of enzymes and protein complexes. Our focus will be on flavin-based electron bifurcating enzymes, but the broad applicability of the techniques will be showcased.},
doi = {10.3389/fmicb.2018.01397},
journal = {Frontiers in Microbiology},
number = ,
volume = 9,
place = {United States},
year = {2018},
month = {7}
}

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