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Title: Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes

Abstract

Duckweed farming can be a sustainable practice for biofuel production, animal feed supplement, and wastewater treatment, although large scale production remains a challenge. Plant growth promoting bacteria (PGPB) have been shown to improve plant health by producing phytohormones such as auxin. While some of the mechanisms for plant growth promotion have been characterized in soil epiphytes, more work is necessary to understand how plants may select for bacterial endophytes that have the ability to provide an exogenous source of phytohormones such as auxin. We have isolated and characterized forty-seven potentially endophytic bacteria from surface-sterilized duckweed tissues and screened these bacterial strains for production of indole related compounds using the Salkowski colorimetric assay. Indole-3-acetic acid (IAA), indole-3-lactic acid (ILA), and indole produced by various bacterial isolates were verified by mass spectrometry. Using the Salkowski reagent, we found that 79% of the isolated bacterial strains from our collection may be capable of producing indole related compounds to various extents during in vitro growth. Of these bacteria that are producing indole related compounds, 19% are additionally producing indole. There is an apparent correlation between the type of indole related compound produced by a particular bacteria and the duckweed genus from which the bacterialmore » strain is derived. These results suggest the possible association between different duckweed genera and endophytes that are producing distinct types of secondary metabolites. Understanding the role of indole related compounds during interaction between endophytes and the plant host may be useful to help design synthetic bacterial communities that could target specific or multiple species of duckweed in the future to sustainably enhance plant growth.« less

Authors:
 [1];  [1];  [1];  [1];  [2];  [1]
  1. Rutgers Univ., New Brunswick, NJ (United States). Dept. of Plant Biology
  2. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology
Publication Date:
Research Org.:
Rutgers Univ., New Brunswick, NJ (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1510429
Grant/Contract Number:  
SC0018244
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Chemistry
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2296-2646
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; auxin; Wolffia; duckweeds; indoles; Salkowski assay; endophytes

Citation Formats

Gilbert, Sarah, Xu, Jenny, Acosta, Kenneth, Poulev, Alexander, Lebeis, Sarah, and Lam, Eric. Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes. United States: N. p., 2018. Web. doi:10.3389/fchem.2018.00265.
Gilbert, Sarah, Xu, Jenny, Acosta, Kenneth, Poulev, Alexander, Lebeis, Sarah, & Lam, Eric. Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes. United States. doi:10.3389/fchem.2018.00265.
Gilbert, Sarah, Xu, Jenny, Acosta, Kenneth, Poulev, Alexander, Lebeis, Sarah, and Lam, Eric. Thu . "Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes". United States. doi:10.3389/fchem.2018.00265. https://www.osti.gov/servlets/purl/1510429.
@article{osti_1510429,
title = {Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes},
author = {Gilbert, Sarah and Xu, Jenny and Acosta, Kenneth and Poulev, Alexander and Lebeis, Sarah and Lam, Eric},
abstractNote = {Duckweed farming can be a sustainable practice for biofuel production, animal feed supplement, and wastewater treatment, although large scale production remains a challenge. Plant growth promoting bacteria (PGPB) have been shown to improve plant health by producing phytohormones such as auxin. While some of the mechanisms for plant growth promotion have been characterized in soil epiphytes, more work is necessary to understand how plants may select for bacterial endophytes that have the ability to provide an exogenous source of phytohormones such as auxin. We have isolated and characterized forty-seven potentially endophytic bacteria from surface-sterilized duckweed tissues and screened these bacterial strains for production of indole related compounds using the Salkowski colorimetric assay. Indole-3-acetic acid (IAA), indole-3-lactic acid (ILA), and indole produced by various bacterial isolates were verified by mass spectrometry. Using the Salkowski reagent, we found that 79% of the isolated bacterial strains from our collection may be capable of producing indole related compounds to various extents during in vitro growth. Of these bacteria that are producing indole related compounds, 19% are additionally producing indole. There is an apparent correlation between the type of indole related compound produced by a particular bacteria and the duckweed genus from which the bacterial strain is derived. These results suggest the possible association between different duckweed genera and endophytes that are producing distinct types of secondary metabolites. Understanding the role of indole related compounds during interaction between endophytes and the plant host may be useful to help design synthetic bacterial communities that could target specific or multiple species of duckweed in the future to sustainably enhance plant growth.},
doi = {10.3389/fchem.2018.00265},
journal = {Frontiers in Chemistry},
number = ,
volume = 6,
place = {United States},
year = {2018},
month = {7}
}

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