Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes
Abstract
Duckweed farming can be a sustainable practice for biofuel production, animal feed supplement, and wastewater treatment, although large scale production remains a challenge. Plant growth promoting bacteria (PGPB) have been shown to improve plant health by producing phytohormones such as auxin. While some of the mechanisms for plant growth promotion have been characterized in soil epiphytes, more work is necessary to understand how plants may select for bacterial endophytes that have the ability to provide an exogenous source of phytohormones such as auxin. We have isolated and characterized forty-seven potentially endophytic bacteria from surface-sterilized duckweed tissues and screened these bacterial strains for production of indole related compounds using the Salkowski colorimetric assay. Indole-3-acetic acid (IAA), indole-3-lactic acid (ILA), and indole produced by various bacterial isolates were verified by mass spectrometry. Using the Salkowski reagent, we found that 79% of the isolated bacterial strains from our collection may be capable of producing indole related compounds to various extents during in vitro growth. Of these bacteria that are producing indole related compounds, 19% are additionally producing indole. There is an apparent correlation between the type of indole related compound produced by a particular bacteria and the duckweed genus from which the bacterialmore »
- Authors:
-
- Rutgers Univ., New Brunswick, NJ (United States). Dept. of Plant Biology
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology
- Publication Date:
- Research Org.:
- Rutgers Univ., New Brunswick, NJ (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1510429
- Grant/Contract Number:
- SC0018244
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Chemistry
- Additional Journal Information:
- Journal Volume: 6; Journal ID: ISSN 2296-2646
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; auxin; Wolffia; duckweeds; indoles; Salkowski assay; endophytes
Citation Formats
Gilbert, Sarah, Xu, Jenny, Acosta, Kenneth, Poulev, Alexander, Lebeis, Sarah, and Lam, Eric. Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes. United States: N. p., 2018.
Web. doi:10.3389/fchem.2018.00265.
Gilbert, Sarah, Xu, Jenny, Acosta, Kenneth, Poulev, Alexander, Lebeis, Sarah, & Lam, Eric. Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes. United States. https://doi.org/10.3389/fchem.2018.00265
Gilbert, Sarah, Xu, Jenny, Acosta, Kenneth, Poulev, Alexander, Lebeis, Sarah, and Lam, Eric. Thu .
"Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes". United States. https://doi.org/10.3389/fchem.2018.00265. https://www.osti.gov/servlets/purl/1510429.
@article{osti_1510429,
title = {Bacterial Production of Indole Related Compounds Reveals Their Role in Association Between Duckweeds and Endophytes},
author = {Gilbert, Sarah and Xu, Jenny and Acosta, Kenneth and Poulev, Alexander and Lebeis, Sarah and Lam, Eric},
abstractNote = {Duckweed farming can be a sustainable practice for biofuel production, animal feed supplement, and wastewater treatment, although large scale production remains a challenge. Plant growth promoting bacteria (PGPB) have been shown to improve plant health by producing phytohormones such as auxin. While some of the mechanisms for plant growth promotion have been characterized in soil epiphytes, more work is necessary to understand how plants may select for bacterial endophytes that have the ability to provide an exogenous source of phytohormones such as auxin. We have isolated and characterized forty-seven potentially endophytic bacteria from surface-sterilized duckweed tissues and screened these bacterial strains for production of indole related compounds using the Salkowski colorimetric assay. Indole-3-acetic acid (IAA), indole-3-lactic acid (ILA), and indole produced by various bacterial isolates were verified by mass spectrometry. Using the Salkowski reagent, we found that 79% of the isolated bacterial strains from our collection may be capable of producing indole related compounds to various extents during in vitro growth. Of these bacteria that are producing indole related compounds, 19% are additionally producing indole. There is an apparent correlation between the type of indole related compound produced by a particular bacteria and the duckweed genus from which the bacterial strain is derived. These results suggest the possible association between different duckweed genera and endophytes that are producing distinct types of secondary metabolites. Understanding the role of indole related compounds during interaction between endophytes and the plant host may be useful to help design synthetic bacterial communities that could target specific or multiple species of duckweed in the future to sustainably enhance plant growth.},
doi = {10.3389/fchem.2018.00265},
journal = {Frontiers in Chemistry},
number = ,
volume = 6,
place = {United States},
year = {Thu Jul 12 00:00:00 EDT 2018},
month = {Thu Jul 12 00:00:00 EDT 2018}
}
Web of Science
Works referenced in this record:
Plant growth-promoting bacterium Acinetobacter calcoaceticus P23 increases the chlorophyll content of the monocot Lemna minor (duckweed) and the dicot Lactuca sativa (lettuce)
journal, July 2014
- Suzuki, Wakako; Sugawara, Masayuki; Miwa, Kyoko
- Journal of Bioscience and Bioengineering, Vol. 118, Issue 1
Indoleacetic acid, a product of transferred DNA, inhibits vir gene expression and growth of Agrobacterium tumefaciens C58
journal, March 2006
- Liu, P.; Nester, E. W.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 12
Colorimetric Estimation of Indoleacetic acid
journal, January 1951
- Gordon, Solon A.; Weber, Robert P.
- Plant Physiology, Vol. 26, Issue 1
Comprehensive definition of genome features in Spirodela polyrhiza by high-depth physical mapping and short-read DNA sequencing strategies
journal, February 2017
- Michael, Todd P.; Bryant, Douglas; Gutierrez, Ryan
- The Plant Journal, Vol. 89, Issue 3
Sustainable Biodegradation of Phenol by Acinetobacter calcoaceticus P23 Isolated from the Rhizosphere of Duckweed Lemna aoukikusa
journal, August 2010
- Yamaga, Fumiko; Washio, Kenji; Morikawa, Masaaki
- Environmental Science & Technology, Vol. 44, Issue 16
Structure, variation, and assembly of the root-associated microbiomes of rice
journal, January 2015
- Edwards, Joseph; Johnson, Cameron; Santos-Medellín, Christian
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 8
Review and re-analysis of domain-specific 16S primers
journal, December 2003
- Baker, G. C.; Smith, J. J.; Cowan, D. A.
- Journal of Microbiological Methods, Vol. 55, Issue 3
Growing Duckweed to Recover Nutrients from Wastewaters and for Production of Fuel Ethanol and Animal Feed
journal, January 2009
- Cheng, Jay J.; Stomp, Anne-M.
- CLEAN - Soil, Air, Water, Vol. 37, Issue 1
Indole-3-acetic acid improves Escherichia coli’s defences to stress
journal, March 2006
- Bianco, C.; Imperlini, E.; Calogero, R.
- Archives of Microbiology, Vol. 185, Issue 5
Plant growth-promoting bacterial endophytes
journal, February 2016
- Santoyo, Gustavo; Moreno-Hagelsieb, Gabriel; del Carmen Orozco-Mosqueda, Ma.
- Microbiological Research, Vol. 183
RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes
journal, February 2015
- Brettin, Thomas; Davis, James J.; Disz, Terry
- Scientific Reports, Vol. 5, Issue 1
Indole-3-acetic acid in microbial and microorganism-plant signaling
journal, July 2007
- Spaepen, Stijn; Vanderleyden, Jos; Remans, Roseline
- FEMS Microbiology Reviews, Vol. 31, Issue 4
Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota
journal, August 2012
- Bulgarelli, Davide; Rott, Matthias; Schlaeppi, Klaus
- Nature, Vol. 488, Issue 7409
Defining the core Arabidopsis thaliana root microbiome
journal, August 2012
- Lundberg, Derek S.; Lebeis, Sarah L.; Paredes, Sur Herrera
- Nature, Vol. 488, Issue 7409
Assessment, validation and deployment strategy of a two-barcode protocol for facile genotyping of duckweed species
journal, August 2014
- Borisjuk, N.; Chu, P.; Gutierrez, R.
- Plant Biology, Vol. 17
Associations with rhizosphere bacteria can confer an adaptive advantage to plants
journal, May 2015
- Haney, Cara H.; Samuel, Buck S.; Bush, Jenifer
- Nature Plants, Vol. 1, Issue 6
The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST)
journal, November 2013
- Overbeek, Ross; Olson, Robert; Pusch, Gordon D.
- Nucleic Acids Research, Vol. 42, Issue D1
Auxin and Plant-Microbe Interactions
journal, November 2010
- Spaepen, S.; Vanderleyden, J.
- Cold Spring Harbor Perspectives in Biology, Vol. 3, Issue 4
Indole as an intercellular signal in microbial communities
journal, July 2010
- Lee, Jin-Hyung; Lee, Jintae
- FEMS Microbiology Reviews, Vol. 34, Issue 4
A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria
journal, May 2007
- Chakravorty, Soumitesh; Helb, Danica; Burday, Michele
- Journal of Microbiological Methods, Vol. 69, Issue 2
Dating the Monocot?Dicot Divergence and the Origin of Core Eudicots Using Whole Chloroplast Genomes
journal, April 2004
- Chang, Chien-Chang; Chen, Hsin-Liang; Li, Wen-Hsiung
- Journal of Molecular Evolution, Vol. 58, Issue 4
Indole can act as an extracellular signal to regulate biofilm formation of Escherichia coli and other indole-producing bacteria
journal, July 2003
- Di Martino, P.; Fursy, R.; Bret, L.
- Canadian Journal of Microbiology, Vol. 49, Issue 7
The RAST Server: Rapid Annotations using Subsystems Technology
journal, January 2008
- Aziz, Ramy K.; Bartels, Daniela; Best, Aaron A.
- BMC Genomics, Vol. 9, Issue 1, Article No. 75
Indole-3-acetic acid improves Escherichia coli’s defences to stress
journal, March 2006
- Bianco, C.; Imperlini, E.; Calogero, R.
- Archives of Microbiology, Vol. 185, Issue 5
Possible effects of temperature on bacterial communities in the rhizosphere of rice under different climatic regions
journal, March 2022
- Zhang, Yang; Zhang, Yujie; Xu, Wenjie
- Archives of Microbiology, Vol. 204, Issue 4
Dating the Monocot?Dicot Divergence and the Origin of Core Eudicots Using Whole Chloroplast Genomes
journal, April 2004
- Chang, Chien-Chang; Chen, Hsin-Liang; Li, Wen-Hsiung
- Journal of Molecular Evolution, Vol. 58, Issue 4
Plant growth-promoting bacterium Acinetobacter calcoaceticus P23 increases the chlorophyll content of the monocot Lemna minor (duckweed) and the dicot Lactuca sativa (lettuce)
journal, July 2014
- Suzuki, Wakako; Sugawara, Masayuki; Miwa, Kyoko
- Journal of Bioscience and Bioengineering, Vol. 118, Issue 1
Plant growth-promoting bacterial endophytes
journal, February 2016
- Santoyo, Gustavo; Moreno-Hagelsieb, Gabriel; del Carmen Orozco-Mosqueda, Ma.
- Microbiological Research, Vol. 183
A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria
journal, May 2007
- Chakravorty, Soumitesh; Helb, Danica; Burday, Michele
- Journal of Microbiological Methods, Vol. 69, Issue 2
The relative importance of Lemna gibba L., bacteria and algae for the nitrogen and phosphorus removal in duckweed-covered domestic wastewater
journal, December 1998
- Körner, S.; Vermaat, J. E.
- Water Research, Vol. 32, Issue 12
Sustainable Biodegradation of Phenol by Acinetobacter calcoaceticus P23 Isolated from the Rhizosphere of Duckweed Lemna aoukikusa
journal, August 2010
- Yamaga, Fumiko; Washio, Kenji; Morikawa, Masaaki
- Environmental Science & Technology, Vol. 44, Issue 16
Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota
journal, August 2012
- Bulgarelli, Davide; Rott, Matthias; Schlaeppi, Klaus
- Nature, Vol. 488, Issue 7409
Associations with rhizosphere bacteria can confer an adaptive advantage to plants
journal, May 2015
- Haney, Cara H.; Samuel, Buck S.; Bush, Jenifer
- Nature Plants, Vol. 1, Issue 6
RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes
journal, February 2015
- Brettin, Thomas; Davis, James J.; Disz, Terry
- Scientific Reports, Vol. 5, Issue 1
The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST)
journal, November 2013
- Overbeek, Ross; Olson, Robert; Pusch, Gordon D.
- Nucleic Acids Research, Vol. 42, Issue D1
Organic Acids, Sugars, and l -Tryptophane in Exudates of Vegetables Growing on Stonewool and Their Effects on Activities of Rhizosphere Bacteria
journal, March 2006
- Kamilova, Faina; Kravchenko, Lev V.; Shaposhnikov, Alexander I.
- Molecular Plant-Microbe Interactions, Vol. 19, Issue 3
Colorimetric Estimation of Indoleacetic acid
journal, January 1951
- Gordon, Solon A.; Weber, Robert P.
- Plant Physiology, Vol. 26, Issue 1
Indole as an intercellular signal in microbial communities
journal, July 2010
- Lee, Jin-Hyung; Lee, Jintae
- FEMS Microbiology Reviews, Vol. 34, Issue 4
Assessment, validation and deployment strategy of a two-barcode protocol for facile genotyping of duckweed species
journal, August 2014
- Borisjuk, N.; Chu, P.; Gutierrez, R.
- Plant Biology, Vol. 17
Comprehensive definition of genome features in Spirodela polyrhiza by high-depth physical mapping and short-read DNA sequencing strategies
journal, February 2017
- Michael, Todd P.; Bryant, Douglas; Gutierrez, Ryan
- The Plant Journal, Vol. 89, Issue 3
A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria.
journal, January 1995
- Glickmann, E.; Dessaux, Y.
- Applied and environmental microbiology, Vol. 61, Issue 2
Indole can act as an extracellular signal to regulate biofilm formation of Escherichia coli and other indole-producing bacteria
journal, July 2003
- Di Martino, P.; Fursy, R.; Bret, L.
- Canadian Journal of Microbiology, Vol. 49, Issue 7
The RAST Server: Rapid Annotations using Subsystems Technology
journal, January 2008
- Aziz, Ramy K.; Bartels, Daniela; Best, Aaron A.
- BMC Genomics, Vol. 9, Issue 1, Article No. 75
Works referencing / citing this record:
Achromobacter sp. FB-14 harboring ACC deaminase activity augmented rice growth by upregulating the expression of stress-responsive CIPK genes under salinity stress
journal, December 2019
- Shahid, Muhammad; Shah, Asad Ali; Basit, Farwa
- Brazilian Journal of Microbiology, Vol. 51, Issue 2
Production of Indole-3-Lactic Acid by Bifidobacterium Strains Isolated fromHuman Infants
journal, September 2019
- Sakurai, Takuma; Odamaki, Toshitaka; Xiao, Jin-zhong
- Microorganisms, Vol. 7, Issue 9
Production of Indole-3-Lactic Acid by Bifidobacterium Strains Isolated fromHuman Infants
journal, September 2019
- Sakurai, Takuma; Odamaki, Toshitaka; Xiao, Jin-zhong
- Microorganisms, Vol. 7, Issue 9