How Water Can Affect Keratin: Hydration‐Driven Recovery of Bighorn Sheep ( Ovis Canadensis ) Horns
Abstract
Abstract Keratin is one of the most common structural biopolymers exhibiting high strength, toughness, and low density. It is found in various tissues such as hairs, feathers, horns, and hooves with various functionalities. For instance, horn keratin absorbs a large amount of energy during intraspecific fights. Keratinized tissues are permanent tissues because of their basic composition consisting of dead keratinized cells that are not able to remodel or regrow once broken or damaged. The lack of a self‐healing mechanism presents a problem for horns, as they are under continued high risk from mechanical damage. In the present work, it is shown for the first time that a combination of material architecture and a water‐assisted recovery mechanism, in the horn of bighorn sheep, endows them with shape and mechanical property recoverability after being subjected to severe compressive loading. Moreover, the effect of hydration is unraveled, on the material molecular structure and mechanical behavior, by means of synchrotron wide angle X‐ray diffraction, Fourier transform infrared spectroscopy, nanoindentation, and in situ and ex situ tensile tests. The recovery and remodeling mechanism is anisotropic and quite distinct to the self‐healing of living tissue such as bones.
- Authors:
-
[7]
- Materials Science and Engineering Program University of California San Diego La Jolla CA USA
- Theoretical and Applied Mechanics Program Northwestern University Evanston IL USA
- Department of NanoEngineering University of California San Diego, La Jolla CA USA
- Department of Chemical and Environment Engineering University of California Riverside CA USA
- Department of Materials Science and Engineering University of California Berkeley CA USA
- Theoretical and Applied Mechanics Program Northwestern University Evanston IL USA, Department of Mechanical Engineering Northwestern University Evanston IL USA
- Materials Science and Engineering Program University of California San Diego La Jolla CA USA, Department of Mechanical and Aerospace Engineering University of California San Diego La Jolla CA USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1510092
- Grant/Contract Number:
- DE‐AC02‐05CH11231
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Advanced Functional Materials
- Additional Journal Information:
- Journal Name: Advanced Functional Materials Journal Volume: 29 Journal Issue: 27; Journal ID: ISSN 1616-301X
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- Germany
- Language:
- English
Citation Formats
Huang, Wei, Zaheri, Alireza, Yang, Wen, Kisailus, David, Ritchie, Robert O., Espinosa, Horacio, and McKittrick, Joanna. How Water Can Affect Keratin: Hydration‐Driven Recovery of Bighorn Sheep ( Ovis Canadensis ) Horns. Germany: N. p., 2019.
Web. doi:10.1002/adfm.201901077.
Huang, Wei, Zaheri, Alireza, Yang, Wen, Kisailus, David, Ritchie, Robert O., Espinosa, Horacio, & McKittrick, Joanna. How Water Can Affect Keratin: Hydration‐Driven Recovery of Bighorn Sheep ( Ovis Canadensis ) Horns. Germany. https://doi.org/10.1002/adfm.201901077
Huang, Wei, Zaheri, Alireza, Yang, Wen, Kisailus, David, Ritchie, Robert O., Espinosa, Horacio, and McKittrick, Joanna. Mon .
"How Water Can Affect Keratin: Hydration‐Driven Recovery of Bighorn Sheep ( Ovis Canadensis ) Horns". Germany. https://doi.org/10.1002/adfm.201901077.
@article{osti_1510092,
title = {How Water Can Affect Keratin: Hydration‐Driven Recovery of Bighorn Sheep ( Ovis Canadensis ) Horns},
author = {Huang, Wei and Zaheri, Alireza and Yang, Wen and Kisailus, David and Ritchie, Robert O. and Espinosa, Horacio and McKittrick, Joanna},
abstractNote = {Abstract Keratin is one of the most common structural biopolymers exhibiting high strength, toughness, and low density. It is found in various tissues such as hairs, feathers, horns, and hooves with various functionalities. For instance, horn keratin absorbs a large amount of energy during intraspecific fights. Keratinized tissues are permanent tissues because of their basic composition consisting of dead keratinized cells that are not able to remodel or regrow once broken or damaged. The lack of a self‐healing mechanism presents a problem for horns, as they are under continued high risk from mechanical damage. In the present work, it is shown for the first time that a combination of material architecture and a water‐assisted recovery mechanism, in the horn of bighorn sheep, endows them with shape and mechanical property recoverability after being subjected to severe compressive loading. Moreover, the effect of hydration is unraveled, on the material molecular structure and mechanical behavior, by means of synchrotron wide angle X‐ray diffraction, Fourier transform infrared spectroscopy, nanoindentation, and in situ and ex situ tensile tests. The recovery and remodeling mechanism is anisotropic and quite distinct to the self‐healing of living tissue such as bones.},
doi = {10.1002/adfm.201901077},
journal = {Advanced Functional Materials},
number = 27,
volume = 29,
place = {Germany},
year = {Mon Apr 29 00:00:00 EDT 2019},
month = {Mon Apr 29 00:00:00 EDT 2019}
}
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Works referenced in this record:
Mechanical Properties and the Structure of Hair
journal, November 1950
- Reese, Cecil E.; Eyring, Henry
- Textile Research Journal, Vol. 20, Issue 11
The effect of behaviour and body weight on the mechanical design of horns
journal, February 1985
- Kitchener, Andrew
- Journal of Zoology, Vol. 205, Issue 2
Infrared spectroscopy of proteins
journal, September 2007
- Barth, Andreas
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1767, Issue 9
A mechanical property evaluation of graded density Al-Si10-Mg lattice structures manufactured by selective laser melting
journal, July 2016
- Maskery, I.; Aboulkhair, N. T.; Aremu, A. O.
- Materials Science and Engineering: A, Vol. 670
The structure of the “amorphous” matrix of keratins
journal, May 2017
- Kadir, Murat; Wang, Xinwei; Zhu, Bowen
- Journal of Structural Biology, Vol. 198, Issue 2
Deformation and energy absorption diagrams for cellular solids
journal, November 1984
- Maiti, S. K.; Gibson, L. J.; Ashby, M. F.
- Acta Metallurgica, Vol. 32, Issue 11
Oriented Structure in Human Stratum Corneum Revealed by X-Ray Diffraction
journal, January 1991
- Garson, Jean-Claude; Doucet, Jean; Lévêque, Jean-Luc
- Journal of Investigative Dermatology, Vol. 96, Issue 1
Effect of water on the linear viscoelasticity of horn sheath keratin
journal, March 1987
- Kitchener, Andrew
- Journal of Materials Science Letters, Vol. 6, Issue 3
Molecular design of the α–keratin composite: insights from a matrix–free model, hagfish slime threads
journal, February 2004
- Fudge, Douglas S.; Gosline, John M.
- Proceedings of the Royal Society of London. Series B: Biological Sciences, Vol. 271, Issue 1536
Mechanical adaptability of the Bouligand-type structure in natural dermal armour
journal, October 2013
- Zimmermann, Elizabeth A.; Gludovatz, Bernd; Schaible, Eric
- Nature Communications, Vol. 4, Issue 1
Fracture toughness of horns and a reinterpretation of the horning behaviour of bovids
journal, December 1987
- Kitchener, Andrew
- Journal of Zoology, Vol. 213, Issue 4
Some Mechanical Properties of Wool Fibers in the "Hookean" Region from Zero to 100% Relative Humidity
journal, June 1971
- Feughelman, M.; Robinson, M. S.
- Textile Research Journal, Vol. 41, Issue 6
Pangolin armor: Overlapping, structure, and mechanical properties of the keratinous scales
journal, September 2016
- Wang, Bin; Yang, Wen; Sherman, Vincent R.
- Acta Biomaterialia, Vol. 41
Analysis of nanoindentation creep for polymeric materials
journal, April 2004
- Yang, Shuang; Zhang, Yong-Wei; Zeng, Kaiyang
- Journal of Applied Physics, Vol. 95, Issue 7
Composite theory and the effect of water on the stiffness of horn keratin
journal, April 1987
- Kitchener, Andrew; Vincent, Julian F. V.
- Journal of Materials Science, Vol. 22, Issue 4
Functional design of horse hoof keratin: the modulation of mechanical properties through hydration effects
journal, July 1987
- Bertram, J. E.; Gosline, J. M.
- Journal of Experimental Biology, Vol. 130, Issue 1
Keratin: Structure, mechanical properties, occurrence in biological organisms, and efforts at bioinspiration
journal, March 2016
- Wang, Bin; Yang, Wen; McKittrick, Joanna
- Progress in Materials Science, Vol. 76
The Intermediate Filament Architecture as Determined by X-Ray Diffraction Modeling of Hard α-Keratin
journal, June 2004
- Er Rafik, Mériem; Doucet, Jean; Briki, Fatma
- Biophysical Journal, Vol. 86, Issue 6
The mechanical efficiency of natural materials
journal, July 2004
- Wegst, U. G. K.; Ashby, M. F.
- Philosophical Magazine, Vol. 84, Issue 21
Water-assisted self-healing and property recovery in a natural dermal armor of pangolin scales
journal, March 2016
- Liu, Z. Q.; Jiao, D.; Weng, Z. Y.
- Journal of the Mechanical Behavior of Biomedical Materials, Vol. 56
Insight into differences in nanoindentation properties of bone
journal, February 2013
- Rodriguez-Florez, Naiara; Oyen, Michelle L.; Shefelbine, Sandra J.
- Journal of the Mechanical Behavior of Biomedical Materials, Vol. 18
Measurement of Creep Compliance of Solid Polymers by Nanoindentation
journal, January 2003
- Lu, H.; Wang, B.; Ma, J.
- Mechanics of Time-Dependent Materials, Vol. 7, Issue 3/4
Mechanism study of biopolymer hair as a coupled thermo-water responsive smart material
journal, February 2017
- Xiao, Xueliang; Zhou, Hongtao; Qian, Kun
- Smart Materials and Structures, Vol. 26, Issue 3
Biological materials: Functional adaptations and bioinspired designs
journal, November 2012
- Chen, Po-Yu; McKittrick, Joanna; Meyers, Marc André
- Progress in Materials Science, Vol. 57, Issue 8
Analysis of the Humidity-Time Superposition for Wool Fibers
journal, December 1985
- Wortmann, F. -J.; De Jong, S.
- Textile Research Journal, Vol. 55, Issue 12
Microstructure and mechanical properties of different keratinous horns
journal, June 2018
- Zhang, Yuchen; Huang, Wei; Hayashi, Cheryl
- Journal of The Royal Society Interface, Vol. 15, Issue 143
An analysis of the forces of fighting of the blackbuck ( Antilope cervicapra ) and the bighorn sheep ( Ovis canadensis ) and the mechanical design of the horn of bovids
journal, January 1988
- Kitchener, Andrew
- Journal of Zoology, Vol. 214, Issue 1
The effects of water and microstructure on the mechanical properties of bighorn sheep (Ovis canadensis) horn keratin
journal, March 2011
- Trim, Michael W.; Horstemeyer, M. F.; Rhee, Hongjoo
- Acta Biomaterialia, Vol. 7, Issue 3
Investigating the microstructure of keratin extracted from wool: Peptide sequence (MALDI-TOF/TOF) and protein conformation (FTIR)
journal, April 2010
- Cardamone, Jeanette M.
- Journal of Molecular Structure, Vol. 969, Issue 1-3
A multi-scale approach to understand the mechanobiology of intermediate filaments
journal, January 2010
- Qin, Zhao; Buehler, Markus J.; Kreplak, Laurent
- Journal of Biomechanics, Vol. 43, Issue 1
Characterization of polymeric structural foams under compressive impact loading by means of energy-absorption diagram
journal, May 2001
- Avalle, M.; Belingardi, G.; Montanini, R.
- International Journal of Impact Engineering, Vol. 25, Issue 5
Nanoconfinement controls stiffness, strength and mechanical toughness of β-sheet crystals in silk
journal, March 2010
- Keten, Sinan; Xu, Zhiping; Ihle, Britni
- Nature Materials, Vol. 9, Issue 4
Energy absorbent natural materials and bioinspired design strategies: A review
journal, April 2010
- McKittrick, J.; Chen, P. -Y.; Tombolato, L.
- Materials Science and Engineering: C, Vol. 30, Issue 3
Viscoelastic properties of α-keratin fibers in hair
journal, December 2017
- Yu, Yang; Yang, Wen; André Meyers, Marc
- Acta Biomaterialia, Vol. 64
Effect of shampoo, conditioner and permanent waving on the molecular structure of human hair
journal, January 2015
- Zhang, Yuchen; Alsop, Richard J.; Soomro, Asfia
- PeerJ, Vol. 3
The structure of people’s hair
journal, January 2014
- Yang, Fei-Chi; Zhang, Yuchen; Rheinstädter, Maikel C.
- PeerJ, Vol. 2
Animal Hairs as Water-stimulated Shape Memory Materials: Mechanism and Structural Networks in Molecular Assemblies
journal, May 2016
- Xiao, Xueliang; Hu, Jinlian
- Scientific Reports, Vol. 6, Issue 1
Biological materials: Structure and mechanical properties
journal, January 2008
- Meyers, Marc André; Chen, Po-Yu; Lin, Albert Yu-Min
- Progress in Materials Science, Vol. 53, Issue 1
Horn and horn core trabecular bone of bighorn sheep rams absorbs impact energy and reduces brain cavity accelerations during high impact ramming of the skull
journal, October 2016
- Drake, Aaron; Haut Donahue, Tammy L.; Stansloski, Mitchel
- Acta Biomaterialia, Vol. 44
Structural Design Elements in Biological Materials: Application to Bioinspiration
journal, August 2015
- Naleway, Steven E.; Porter, Michael M.; McKittrick, Joanna
- Advanced Materials, Vol. 27, Issue 37
The molecular configuration of α-keratin
journal, October 1961
- Fraser, R. D. B.; MacRae, T. P.
- Journal of Molecular Biology, Vol. 3, Issue 5
Hair—the most sophisticated biological composite material
journal, January 2007
- Popescu, Crisan; Höcker, Hartwig
- Chemical Society Reviews, Vol. 36, Issue 8
The effect of holding time on nanoindentation measurements of creep in bone
journal, April 2011
- Wu, Ziheng; Baker, Tyler A.; Ovaert, Timothy C.
- Journal of Biomechanics, Vol. 44, Issue 6
Remarkable shape memory effect of a natural biopolymer in aqueous environment
journal, October 2015
- Liu, Z. Q.; Jiao, D.; Zhang, Z. F.
- Biomaterials, Vol. 65
Lamellae spatial distribution modulates fracture behavior and toughness of african pangolin scales
journal, December 2017
- Chon, Michael J.; Daly, Matthew; Wang, Bin
- Journal of the Mechanical Behavior of Biomedical Materials, Vol. 76
Designing the energy absorption capacity of functionally graded foam materials
journal, May 2009
- Cui, Liang; Kiernan, Stephen; Gilchrist, Michael D.
- Materials Science and Engineering: A, Vol. 507, Issue 1-2
The coiled-coil model of α-keratin structure
journal, October 1964
- Fraser, R. D. B.; MacRae, T. P.; Miller, A.
- Journal of Molecular Biology, Vol. 10, Issue 1
Hierarchies, multiple energy barriers, and robustness govern the fracture mechanics of -helical and beta-sheet protein domains
journal, October 2007
- Ackbarow, T.; Chen, X.; Keten, S.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 42
Hydration-induced nano- to micro-scale self-recovery of the tooth enamel of the giant panda
journal, November 2018
- Liu, Zengqian; Weng, Zhaoyong; Zhai, Zhao-Feng
- Acta Biomaterialia, Vol. 81
Measuring the constitutive behavior of viscoelastic solids in the time and frequency domain using flat punch nanoindentation
journal, March 2009
- Herbert, E. G.; Oliver, W. C.; Lumsdaine, A.
- Journal of Materials Research, Vol. 24, Issue 3
Hierarchical structure and compressive deformation mechanisms of bighorn sheep (Ovis canadensis) horn
journal, December 2017
- Huang, Wei; Zaheri, Alireza; Jung, Jae-Young
- Acta Biomaterialia, Vol. 64
On the tear resistance of skin
journal, March 2015
- Yang, Wen; Sherman, Vincent R.; Gludovatz, Bernd
- Nature Communications, Vol. 6, Issue 1
Moisture, anisotropy, stress state, and strain rate effects on bighorn sheep horn keratin mechanical properties
journal, January 2017
- Johnson, K. L.; Trim, M. W.; Francis, D. K.
- Acta Biomaterialia, Vol. 48
The Mechanical Properties of Natural Materials. II. Microstructures for Mechanical Efficiency
journal, July 1995
- Gibson, L. J.; Ashby, M. F.; Karam, G. N.
- Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 450, Issue 1938
Microstructure, elastic properties and deformation mechanisms of horn keratin
journal, February 2010
- Tombolato, Luca; Novitskaya, Ekaterina E.; Chen, Po-Yu
- Acta Biomaterialia, Vol. 6, Issue 2
Bioinspired structural materials
journal, October 2014
- Wegst, Ulrike G. K.; Bai, Hao; Saiz, Eduardo
- Nature Materials, Vol. 14, Issue 1
Deformation mechanisms and energy absorption of polystyrene foams for protective helmets
journal, January 2002
- Di Landro, Luca; Sala, Giuseppe; Olivieri, Daniela
- Polymer Testing, Vol. 21, Issue 2
Modelling the mechanical behavior of cellular materials
journal, March 1989
- Gibson, L. J.
- Materials Science and Engineering: A, Vol. 110