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Title: Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals

Abstract

Microbial production of oleochemicals from renewable feedstocks remains an attractive route to produce high-energy density, liquid transportation fuels and high-value chemical products. Metabolic engineering strategies have been applied to demonstrate production of a wide range of oleochemicals, including free fatty acids, fatty alcohols, esters, olefins, alkanes, ketones, and polyesters in both bacteria and yeast. The majority of these demonstrations synthesized products containing long-chain fatty acids. These successes motivated additional effort to produce analogous molecules comprised of medium-chain fatty acids, molecules that are less common in natural oils and therefore of higher commercial value. Substantial progress has been made towards producing a subset of these chemicals, but significant work remains for most. The other primary challenge to producing oleochemicals in microbes is improving the performance, in terms of yield, rate, and titer, of biocatalysts such that economic large-scale processes are feasible. Common metabolic engineering strategies include blocking pathways that compete with synthesis of oleochemical building blocks and/or consume products, pulling flux through pathways by removing regulatory signals, pushing flux into biosynthesis by overexpressing rate-limiting enzymes, and engineering cells to tolerate the presence of oleochemical products. In this review, we describe the basic fundamentals of oleochemical synthesis and summarize advances since 2013more » towards improving performance of heterotrophic microbial cell factories.« less

Authors:
 [1];  [2]
  1. Univ. of Wisconsin, Madison, WI (United States). Dept. of Chemical and Biological Engineering, and DOE Center for Advanced Bioenergy and Bioproducts Innovation
  2. Univ. of Wisconsin, Madison, WI (United States). Dept. of Chemical and Biological Engineering, DOE Center for Advanced Bioenergy and Bioproducts Innovation, and Microbiology Doctoral Training Program
Publication Date:
Research Org.:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF)
OSTI Identifier:
1509679
Grant/Contract Number:  
SC0018420
Resource Type:
Accepted Manuscript
Journal Name:
Metabolic Engineering
Additional Journal Information:
Journal Name: Metabolic Engineering; Journal ID: ISSN 1096-7176
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Oleochemical; Escherichia coli; Yarrowia lipolytica; Saccharomyces cerevisiae; Metabolic engineering; Fatty acid; Enzyme engineering; Biodiesel, Beta-oxidation; Thioesterase

Citation Formats

Yan, Qiang, and Pfleger, Brian F. Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals. United States: N. p., 2019. Web. doi:10.1016/j.ymben.2019.04.009.
Yan, Qiang, & Pfleger, Brian F. Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals. United States. doi:10.1016/j.ymben.2019.04.009.
Yan, Qiang, and Pfleger, Brian F. Mon . "Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals". United States. doi:10.1016/j.ymben.2019.04.009.
@article{osti_1509679,
title = {Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals},
author = {Yan, Qiang and Pfleger, Brian F.},
abstractNote = {Microbial production of oleochemicals from renewable feedstocks remains an attractive route to produce high-energy density, liquid transportation fuels and high-value chemical products. Metabolic engineering strategies have been applied to demonstrate production of a wide range of oleochemicals, including free fatty acids, fatty alcohols, esters, olefins, alkanes, ketones, and polyesters in both bacteria and yeast. The majority of these demonstrations synthesized products containing long-chain fatty acids. These successes motivated additional effort to produce analogous molecules comprised of medium-chain fatty acids, molecules that are less common in natural oils and therefore of higher commercial value. Substantial progress has been made towards producing a subset of these chemicals, but significant work remains for most. The other primary challenge to producing oleochemicals in microbes is improving the performance, in terms of yield, rate, and titer, of biocatalysts such that economic large-scale processes are feasible. Common metabolic engineering strategies include blocking pathways that compete with synthesis of oleochemical building blocks and/or consume products, pulling flux through pathways by removing regulatory signals, pushing flux into biosynthesis by overexpressing rate-limiting enzymes, and engineering cells to tolerate the presence of oleochemical products. In this review, we describe the basic fundamentals of oleochemical synthesis and summarize advances since 2013 towards improving performance of heterotrophic microbial cell factories.},
doi = {10.1016/j.ymben.2019.04.009},
journal = {Metabolic Engineering},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {4}
}

Journal Article:
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This content will become publicly available on April 22, 2020
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