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Title: Fungal endophytes of Populus trichocarpa alter host phenotype, gene expression and rhizobiome composition

Abstract

Mortierella and Ilyonectria include common species of soil fungi which are frequently detected as root endophytes in many plants including Populus spp. However, the ecological roles of these and other endophytic fungi with respect to plant growth and function are still not well understood. The functional ecology of two key taxa from the Populus rhizobiome, Mortierella elongata PMI93 and Ilyonectria europaea PMI82, was studied by coupling forest soil bioassays with environmental metatranscriptomics. Using soil bioassay experiments amended with fungal inoculants, M. elongata was observed to promote the growth of Populus. Here, this response was cultivar independent. In contrast, I. europaea had no visible effect on Populus growth. Metatranscriptomic studies reveal that these fungi impact rhizophytic and endophytic activities in Populus and induce shifts in soil and root microbial communities. Differential expression of core genes in P. trichocarpa roots was observed in response to both fungal species. Expression of Populus genes for lipid signaling and nutrient uptake were up-regulated and expression of genes associated with gibberellin signaling were altered in plants inoculated with M elongata, but not I. europaea. Up-regulation of genes for growth promotion, down-regulation of genes for several LRR-receptors/kinases, and alteration of expression of genes associated with plant defensemore » responses (e.g., JA/ET/SA pathways) also suggest that M. elongata manipulates plant defenses while promoting plant growth.« less

Authors:
 [1];  [2];  [3];  [4];  [5]; ORCiD logo [6]; ORCiD logo [6]; ORCiD logo [6];  [7];  [8];  [4]
  1. Univ. of Florida, Quincy, FL (United States)
  2. Michigan State Univ., East Lansing, MI (United States)
  3. Duke Univ., Durham, NC (United States); Univ. of Arkansas, Fayetteville, AR (United States)
  4. Duke Univ., Durham, NC (United States)
  5. Davis (United States)
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  7. Institut National de la Recherche Agronomique, Champenoux (France)
  8. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1509573
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Molecular Plant-Microbe Interactions
Additional Journal Information:
Journal Name: Molecular Plant-Microbe Interactions; Journal ID: ISSN 0894-0282
Publisher:
APS Press - International Society for Molecular Plant-Microbe Interactions
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Liao, Hui -Ling, Bonito, Gregory, Rojas, J. Alejandro, Hameed, Khalid, Wu, Steven, Schadt, Christopher Warren, Labbe, Jessy L., Tuskan, Gerald A., Martin, Francis M., Grigoriev, Igor V., and Vilgalys, Rytas. Fungal endophytes of Populus trichocarpa alter host phenotype, gene expression and rhizobiome composition. United States: N. p., 2019. Web. doi:10.1094/MPMI-05-18-0133-R.
Liao, Hui -Ling, Bonito, Gregory, Rojas, J. Alejandro, Hameed, Khalid, Wu, Steven, Schadt, Christopher Warren, Labbe, Jessy L., Tuskan, Gerald A., Martin, Francis M., Grigoriev, Igor V., & Vilgalys, Rytas. Fungal endophytes of Populus trichocarpa alter host phenotype, gene expression and rhizobiome composition. United States. doi:10.1094/MPMI-05-18-0133-R.
Liao, Hui -Ling, Bonito, Gregory, Rojas, J. Alejandro, Hameed, Khalid, Wu, Steven, Schadt, Christopher Warren, Labbe, Jessy L., Tuskan, Gerald A., Martin, Francis M., Grigoriev, Igor V., and Vilgalys, Rytas. Wed . "Fungal endophytes of Populus trichocarpa alter host phenotype, gene expression and rhizobiome composition". United States. doi:10.1094/MPMI-05-18-0133-R.
@article{osti_1509573,
title = {Fungal endophytes of Populus trichocarpa alter host phenotype, gene expression and rhizobiome composition},
author = {Liao, Hui -Ling and Bonito, Gregory and Rojas, J. Alejandro and Hameed, Khalid and Wu, Steven and Schadt, Christopher Warren and Labbe, Jessy L. and Tuskan, Gerald A. and Martin, Francis M. and Grigoriev, Igor V. and Vilgalys, Rytas},
abstractNote = {Mortierella and Ilyonectria include common species of soil fungi which are frequently detected as root endophytes in many plants including Populus spp. However, the ecological roles of these and other endophytic fungi with respect to plant growth and function are still not well understood. The functional ecology of two key taxa from the Populus rhizobiome, Mortierella elongata PMI93 and Ilyonectria europaea PMI82, was studied by coupling forest soil bioassays with environmental metatranscriptomics. Using soil bioassay experiments amended with fungal inoculants, M. elongata was observed to promote the growth of Populus. Here, this response was cultivar independent. In contrast, I. europaea had no visible effect on Populus growth. Metatranscriptomic studies reveal that these fungi impact rhizophytic and endophytic activities in Populus and induce shifts in soil and root microbial communities. Differential expression of core genes in P. trichocarpa roots was observed in response to both fungal species. Expression of Populus genes for lipid signaling and nutrient uptake were up-regulated and expression of genes associated with gibberellin signaling were altered in plants inoculated with M elongata, but not I. europaea. Up-regulation of genes for growth promotion, down-regulation of genes for several LRR-receptors/kinases, and alteration of expression of genes associated with plant defense responses (e.g., JA/ET/SA pathways) also suggest that M. elongata manipulates plant defenses while promoting plant growth.},
doi = {10.1094/MPMI-05-18-0133-R},
journal = {Molecular Plant-Microbe Interactions},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {1}
}

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This content will become publicly available on January 30, 2020
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