Downregulation of pectin biosynthesis gene GAUT4 leads to reduced ferulate and lignin-carbohydrate cross-linking in switchgrass
Abstract
Knockdown (KD) expression of GAlactUronosylTransferase 4 (GAUT4) in switchgrass improves sugar yield and ethanol production from the biomass. The reduced recalcitrance of GAUT4-KD transgenic biomass is associated with reduced cell wall pectic homogalacturonan and rhamnogalacturonan II content and cross-linking, and the associated increases in accessibility of cellulose to enzymatic deconstruction. To further probe the molecular basis for the reduced recalcitrance of GAUT4-KD biomass, potential recalcitrance-related factors including the physicochemical properties of lignin and hemicellulose are investigated. We show that the transgenic switchgrass have a lower abundance of ferulate and lignin-carbohydrate complex cross-linkages, reduced amounts of residual arabinan and xylan in lignin-enriched fractions after enzymatic hydrolysis, and greater coalescence and migration of lignin after hydrothermal pretreatment in comparison to the wild-type switchgrass control. The results reveal the roles of both decreased lignin-polymer and pectin cross-links in the reduction of recalcitrance in PvGAUT4-KD switchgrass.
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF); Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1619630
- Alternate Identifier(s):
- OSTI ID: 1509568; OSTI ID: 1964097
- Grant/Contract Number:
- AC05-00OR22725; SC0015662
- Resource Type:
- Published Article
- Journal Name:
- Communications Biology
- Additional Journal Information:
- Journal Name: Communications Biology Journal Volume: 2 Journal Issue: 1; Journal ID: ISSN 2399-3642
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Agriculture; Biofuels
Citation Formats
Li, Mi, Yoo, Chang Geun, Pu, Yunqiao, Biswal, Ajaya K., Tolbert, Allison K., Mohnen, Debra, and Ragauskas, Arthur J. Downregulation of pectin biosynthesis gene GAUT4 leads to reduced ferulate and lignin-carbohydrate cross-linking in switchgrass. United Kingdom: N. p., 2019.
Web. doi:10.1038/s42003-018-0265-6.
Li, Mi, Yoo, Chang Geun, Pu, Yunqiao, Biswal, Ajaya K., Tolbert, Allison K., Mohnen, Debra, & Ragauskas, Arthur J. Downregulation of pectin biosynthesis gene GAUT4 leads to reduced ferulate and lignin-carbohydrate cross-linking in switchgrass. United Kingdom. https://doi.org/10.1038/s42003-018-0265-6
Li, Mi, Yoo, Chang Geun, Pu, Yunqiao, Biswal, Ajaya K., Tolbert, Allison K., Mohnen, Debra, and Ragauskas, Arthur J. Thu .
"Downregulation of pectin biosynthesis gene GAUT4 leads to reduced ferulate and lignin-carbohydrate cross-linking in switchgrass". United Kingdom. https://doi.org/10.1038/s42003-018-0265-6.
@article{osti_1619630,
title = {Downregulation of pectin biosynthesis gene GAUT4 leads to reduced ferulate and lignin-carbohydrate cross-linking in switchgrass},
author = {Li, Mi and Yoo, Chang Geun and Pu, Yunqiao and Biswal, Ajaya K. and Tolbert, Allison K. and Mohnen, Debra and Ragauskas, Arthur J.},
abstractNote = {Knockdown (KD) expression of GAlactUronosylTransferase 4 (GAUT4) in switchgrass improves sugar yield and ethanol production from the biomass. The reduced recalcitrance of GAUT4-KD transgenic biomass is associated with reduced cell wall pectic homogalacturonan and rhamnogalacturonan II content and cross-linking, and the associated increases in accessibility of cellulose to enzymatic deconstruction. To further probe the molecular basis for the reduced recalcitrance of GAUT4-KD biomass, potential recalcitrance-related factors including the physicochemical properties of lignin and hemicellulose are investigated. We show that the transgenic switchgrass have a lower abundance of ferulate and lignin-carbohydrate complex cross-linkages, reduced amounts of residual arabinan and xylan in lignin-enriched fractions after enzymatic hydrolysis, and greater coalescence and migration of lignin after hydrothermal pretreatment in comparison to the wild-type switchgrass control. The results reveal the roles of both decreased lignin-polymer and pectin cross-links in the reduction of recalcitrance in PvGAUT4-KD switchgrass.},
doi = {10.1038/s42003-018-0265-6},
journal = {Communications Biology},
number = 1,
volume = 2,
place = {United Kingdom},
year = {Thu Jan 17 00:00:00 EST 2019},
month = {Thu Jan 17 00:00:00 EST 2019}
}
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https://doi.org/10.1038/s42003-018-0265-6
https://doi.org/10.1038/s42003-018-0265-6
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Cited by: 23 works
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Figures / Tables:
Figure 1: The molecular weights and glycosyl residue composition of the hemicellulose fractions from switchgrass. Number-average (Mn) and weight-average (Mw) molecular weights of the alkaline-extracted hemicellulose-enriched fractions isolated from GAUT4-KD (2A, 2B, and 4A) lines and wild-type (WT) control. Ara: arabinose; Rha: rhamnose; Xyl: xylose; GalA: galacturonic acid; Man: mannose;more »
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