Sulfate-Reducing Bacteria That Produce Exopolymers Thrive in the Calcifying Zone of a Hypersaline Cyanobacterial Mat

doi:10.3389/fmicb.2019.00862

Title: Sulfate-Reducing Bacteria That Produce Exopolymers Thrive in the Calcifying Zone of a Hypersaline Cyanobacterial Mat

Abstract

Calcifying microbial mats in hypersaline environments are important model systems for the study of the earliest ecosystems on Earth that started to appear more than three billion years ago and have been preserved in the fossil record as laminated lithified structures known as stromatolites. It is believed that sulfate-reducing bacteria play a pivotal role in the lithification process by increasing the saturation index of calcium minerals within the mat. Strain L21-Syr-AB ^T was isolated from anoxic samples of a several centimeters-thick microbialite-forming cyanobacterial mat of a hypersaline lake on the Kiritimati Atoll (Kiribati, Central Pacific). The novel isolate was assigned to the family Desulfovibrionaceae within the Deltaproteobacteria. Available 16S rRNA-based population surveys obtained from discrete layers of the mat indicate that the occurrence of a species-level clade represented by strain L21-Syr-AB ^T is restricted to a specific layer of the suboxic zone, which is characterized by the presence of aragonitic spherulites. To elucidate a possible function of this sulfate-reducing bacterium in the mineral formation within the mat a comprehensive phenotypic characterization was combined with the results of a comparative genome analysis. Among the determined traits of strain L21-Syr-AB ^T, several features were identified that could play a role in themore »« less

Authors:

Spring, Stefan ^[1]; Sorokin, Dimitry Y. ^[2]; Verbarg, Susanne ^[1]; Rohde, Manfred ^[3]; Woyke, Tanja ^[4]; Kyrpides, Nikos C. ^[4]

Leibniz Inst. DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig (Germany)
Winogradsky Inst. of Microbiology, Research Centre of Biotechnology, Moscow (Russia); Delft Univ. of Technology (Netherlands)
Helmholtz Centre for Infection Research, Braunschweig (Germany). Central Facility for Microscopy
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

Publication Date:: 2019-04-24

Research Org.:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1508654

Alternate Identifier(s):: OSTI ID: 1560580

Grant/Contract Number:: AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Frontiers in Microbiology

Additional Journal Information:: Journal Volume: 10; Journal Issue: APR; Journal ID: ISSN 1664-302X

Publisher:: Frontiers Research Foundation

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; stromatolites; lithification; biofilm; alkalinity engine; sulfate reduction

Citation Formats


                    Spring, Stefan, Sorokin, Dimitry Y., Verbarg, Susanne, Rohde, Manfred, Woyke, Tanja, and Kyrpides, Nikos C. Sulfate-Reducing Bacteria That Produce Exopolymers Thrive in the Calcifying Zone of a Hypersaline Cyanobacterial Mat.  United States: N. p., 2019. 
        Web.  doi:10.3389/fmicb.2019.00862.

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                    Spring, Stefan, Sorokin, Dimitry Y., Verbarg, Susanne, Rohde, Manfred, Woyke, Tanja, & Kyrpides, Nikos C. Sulfate-Reducing Bacteria That Produce Exopolymers Thrive in the Calcifying Zone of a Hypersaline Cyanobacterial Mat.  United States.  doi:10.3389/fmicb.2019.00862.

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                    Spring, Stefan, Sorokin, Dimitry Y., Verbarg, Susanne, Rohde, Manfred, Woyke, Tanja, and Kyrpides, Nikos C. Wed .  
        "Sulfate-Reducing Bacteria That Produce Exopolymers Thrive in the Calcifying Zone of a Hypersaline Cyanobacterial Mat".  United States.  doi:10.3389/fmicb.2019.00862.

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@article{osti_1508654,

  title        = {Sulfate-Reducing Bacteria That Produce Exopolymers Thrive in the Calcifying Zone of a Hypersaline Cyanobacterial Mat},

  author       = {Spring, Stefan and Sorokin, Dimitry Y. and Verbarg, Susanne and Rohde, Manfred and Woyke, Tanja and Kyrpides, Nikos C.},

  abstractNote = {Calcifying microbial mats in hypersaline environments are important model systems for the study of the earliest ecosystems on Earth that started to appear more than three billion years ago and have been preserved in the fossil record as laminated lithified structures known as stromatolites. It is believed that sulfate-reducing bacteria play a pivotal role in the lithification process by increasing the saturation index of calcium minerals within the mat. Strain L21-Syr-ABT was isolated from anoxic samples of a several centimeters-thick microbialite-forming cyanobacterial mat of a hypersaline lake on the Kiritimati Atoll (Kiribati, Central Pacific). The novel isolate was assigned to the family Desulfovibrionaceae within the Deltaproteobacteria. Available 16S rRNA-based population surveys obtained from discrete layers of the mat indicate that the occurrence of a species-level clade represented by strain L21-Syr-ABT is restricted to a specific layer of the suboxic zone, which is characterized by the presence of aragonitic spherulites. To elucidate a possible function of this sulfate-reducing bacterium in the mineral formation within the mat a comprehensive phenotypic characterization was combined with the results of a comparative genome analysis. Among the determined traits of strain L21-Syr-ABT, several features were identified that could play a role in the precipitation of calcium carbonate: (i) the potential deacetylation of polysaccharides and consumption of substrates such as lactate and sulfate could mobilize free calcium; (ii) under conditions that favor the utilization of formate and hydrogen, the alkalinity engine within the mat is stimulated, thereby increasing the availability of carbonate; (iii) the production of extracellular polysaccharides could provide nucleation sites for calcium mineralization. In addition, our data suggest the proposal of the novel species and genus Desulfohalovibrio reitneri represented by the type strain L21-Syr-ABT (=DSM 26903T = JCM 18662T).},

  doi          = {10.3389/fmicb.2019.00862},

  journal      = {Frontiers in Microbiology},

  number       = APR,

  volume       = 10,

  place        = {United States},

  year         = {2019},

  month        = {4}

}

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DOI: 10.3389/fmicb.2019.00862

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Works referenced in this record:

Draft Genome Sequences for Three Mercury-Methylating, Sulfate-Reducing Bacteria
journal, June 2013

Brown, S. D.; Hurt, R. A.; Gilmour, C. C.
Genome Announcements, Vol. 1, Issue 4, Article No. e00618-13
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The Genetic Basis for Bacterial Mercury Methylation
journal, February 2013

Parks, J. M.; Johs, A.; Podar, M.
Science, Vol. 339, Issue 6125, p. 1332-1335
DOI: 10.1126/science.1230667

The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes
preprint, March 2016

Rodriguez-R, Luis M.; Konstantinidis, Konstantinos T.
PeerJ Preprints 4:e1900v1
DOI: 10.7287/peerj.preprints.1900v1

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Sulfate-Reducing Bacteria That Produce Exopolymers Thrive in the Calcifying Zone of a Hypersaline Cyanobacterial Mat

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Similar Records

Title: Sulfate-Reducing Bacteria That Produce Exopolymers Thrive in the Calcifying Zone of a Hypersaline Cyanobacterial Mat

Abstract

Citation Formats

Draft Genome Sequences for Three Mercury-Methylating, Sulfate-Reducing Bacteria journal, June 2013

The Genetic Basis for Bacterial Mercury Methylation journal, February 2013

The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes preprint, March 2016

Draft Genome Sequences for Three Mercury-Methylating, Sulfate-Reducing Bacteria
journal, June 2013

The Genetic Basis for Bacterial Mercury Methylation
journal, February 2013

The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes
preprint, March 2016