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Title: Phycobilisomes Harbor FNR L in Cyanobacteria

Abstract

Cyanobacterial phycobilisomes (PBSs) are photosynthetic antenna complexes that harvest light energy and supply it to two reaction centers (RCs) where photochemistry starts. PBSs can be classified into two types, depending on the presence of allophycocyanin (APC): CpcG-PBS and CpcL-PBS. Because the accurate protein composition of CpcL-PBS remains unclear, we describe here its isolation and characterization from the cyanobacterium Synechocystis sp. strain 6803. We found that ferredoxin-NADP⁺ oxidoreductase (or FNRL), an enzyme involved in both cyclic electron transport and the terminal step of the electron transport chain in oxygenic photosynthesis, is tightly associated with CpcL-PBS as well as with CpcG-PBS. Room temperature and low-temperature fluorescence analyses show a red-shifted emission at 669 nm in CpcL-PBS as a terminal energy emitter without APC. SDS-PAGE and quantitative mass spectrometry reveal an increased content of FNRL and CpcC2, a rod linker protein, in CpcL-PBS compared to that of CpcG-PBS rods, indicative of an elongated CpcL-PBS rod length and its potential functional differences from CpcGPBS. Furthermore, we combined isotope-encoded cross-linking mass spectrometry with computational protein structure predictions and structural modeling to produce an FNRL-PBS binding model that is supported by two cross-links between K69 of FNRL and the N terminus of CpcB, one component inmore » PBS, in both CpcG-PBS and CpcL-PBS (cross-link 1), and between the N termini of FNRL and CpcB (cross-link 2). Our data provide a novel functional assembly form of phycobiliproteins and a molecular-level description of the close association of FNRL with phycocyanin in both CpcG-PBS and CpcL-PBS.« less

Authors:
ORCiD logo; ; ; ; ; ; ; ORCiD logo; ; ORCiD logo;
Publication Date:
Research Org.:
Washington Univ., St. Louis, MO (United States); Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division; National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH)
OSTI Identifier:
1508182
Alternate Identifier(s):
OSTI ID: 1626117
Grant/Contract Number:  
SC0001035; FG02-99ER20350; 2P41GM103422
Resource Type:
Published Article
Journal Name:
mBio (Online)
Additional Journal Information:
Journal Name: mBio (Online) Journal Volume: 10 Journal Issue: 2; Journal ID: ISSN 2150-7511
Publisher:
American Society for Microbiology
Country of Publication:
Country unknown/Code not available
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; microbiology; CpcL-PBS; isotopic cross-linking; photosynthesis; mass spectrometry

Citation Formats

Liu, Haijun, Weisz, Daniel A., Zhang, Mengru M., Cheng, Ming, Zhang, Bojie, Zhang, Hao, Gerstenecker, Gary S., Pakrasi, Himadri B., Gross, Michael L., Blankenship, Robert E., and McFall-Ngai, ed., Margaret J. Phycobilisomes Harbor FNR L in Cyanobacteria. Country unknown/Code not available: N. p., 2019. Web. https://doi.org/10.1128/mBio.00669-19.
Liu, Haijun, Weisz, Daniel A., Zhang, Mengru M., Cheng, Ming, Zhang, Bojie, Zhang, Hao, Gerstenecker, Gary S., Pakrasi, Himadri B., Gross, Michael L., Blankenship, Robert E., & McFall-Ngai, ed., Margaret J. Phycobilisomes Harbor FNR L in Cyanobacteria. Country unknown/Code not available. https://doi.org/10.1128/mBio.00669-19
Liu, Haijun, Weisz, Daniel A., Zhang, Mengru M., Cheng, Ming, Zhang, Bojie, Zhang, Hao, Gerstenecker, Gary S., Pakrasi, Himadri B., Gross, Michael L., Blankenship, Robert E., and McFall-Ngai, ed., Margaret J. Tue . "Phycobilisomes Harbor FNR L in Cyanobacteria". Country unknown/Code not available. https://doi.org/10.1128/mBio.00669-19.
@article{osti_1508182,
title = {Phycobilisomes Harbor FNR L in Cyanobacteria},
author = {Liu, Haijun and Weisz, Daniel A. and Zhang, Mengru M. and Cheng, Ming and Zhang, Bojie and Zhang, Hao and Gerstenecker, Gary S. and Pakrasi, Himadri B. and Gross, Michael L. and Blankenship, Robert E. and McFall-Ngai, ed., Margaret J.},
abstractNote = {Cyanobacterial phycobilisomes (PBSs) are photosynthetic antenna complexes that harvest light energy and supply it to two reaction centers (RCs) where photochemistry starts. PBSs can be classified into two types, depending on the presence of allophycocyanin (APC): CpcG-PBS and CpcL-PBS. Because the accurate protein composition of CpcL-PBS remains unclear, we describe here its isolation and characterization from the cyanobacterium Synechocystis sp. strain 6803. We found that ferredoxin-NADP⁺ oxidoreductase (or FNRL), an enzyme involved in both cyclic electron transport and the terminal step of the electron transport chain in oxygenic photosynthesis, is tightly associated with CpcL-PBS as well as with CpcG-PBS. Room temperature and low-temperature fluorescence analyses show a red-shifted emission at 669 nm in CpcL-PBS as a terminal energy emitter without APC. SDS-PAGE and quantitative mass spectrometry reveal an increased content of FNRL and CpcC2, a rod linker protein, in CpcL-PBS compared to that of CpcG-PBS rods, indicative of an elongated CpcL-PBS rod length and its potential functional differences from CpcGPBS. Furthermore, we combined isotope-encoded cross-linking mass spectrometry with computational protein structure predictions and structural modeling to produce an FNRL-PBS binding model that is supported by two cross-links between K69 of FNRL and the N terminus of CpcB, one component in PBS, in both CpcG-PBS and CpcL-PBS (cross-link 1), and between the N termini of FNRL and CpcB (cross-link 2). Our data provide a novel functional assembly form of phycobiliproteins and a molecular-level description of the close association of FNRL with phycocyanin in both CpcG-PBS and CpcL-PBS.},
doi = {10.1128/mBio.00669-19},
journal = {mBio (Online)},
number = 2,
volume = 10,
place = {Country unknown/Code not available},
year = {2019},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1128/mBio.00669-19

Citation Metrics:
Cited by: 3 works
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Figures / Tables:

FIG 1 FIG 1: (A) Sucrose density isolation of CpcG-PBS and CpcL-PBS. (B) SDS-PAGE analysis of CpcG-PBS and CpcL-PBS. (C) Mass spectrometry quantification of protein subunits in CpcG-PBS and CpcL-PBS. In the heat map representation, highlighting the component differences, the relevance of effects is related to a ratio, not to a visualmore » difference. Ratio* (PEAKS label-free quantification) indicates the CpcL-PBS/CpcG-PBS ratio. (D) Absorption spectra of the sucrose gradient bands normalized at the absorption maxima. The spectrum difference (Dif) is the difference spectrum between CpcG-PBS and CpcL-PBS. (E and F) Fluorescence emission spectra of CpcG-PBS and CpcL-PBS upon excitation at 580 nm at room temperature (RT) (E) and at 77 K (F).« less

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