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Title: Identifying and creating pathways to improve biological lignin valorization

Abstract

Biological lignin valorization to fuels and value-added chemicals enables sustainable and economic biorefineries. Even though significant progress has been made, several major challenges arose due to high recalcitrance and heterogeneity of lignin, which needs to be addressed to improve lignin processing. This work demonstrates an overview of biological lignin conversion and its regulation from a metabolic engineering and systems biology viewpoint. Biological lignin valorization includes three stages: lignin depolymerization, aromatics degradation, and target product biosynthesis. Ligninolytic microorganisms have an extensive enzymatic toolbox to break down the lignin and convert heterogeneous lignin derivatives to central intermediates, such as protocatechuate or catechol, through a peripheral pathway. These intermediates undergo ring cleavage via the β-ketoadipate pathway and are ultimately transformed into metabolites by yielding acetyl-CoA for internal product biosynthesis, such as triacylglycerols, polyhydroxyalkanoates, etc. Bioprospecting will expand the knowledge base of ligninolytic microbial communities, strains, and enzymes to facilitate the understanding of aromatics metabolism. Systems biology analyses achieve an understanding of molecular and systems-level degradation mechanisms and metabolic pathways of lignin and aromatics. By identifying these mechanisms, synthetic biology provides promising approaches to create the lignin conversion pathways and engineer ligninolytic strains suitable as potential hosts for lignin conversion. Techno-economic analysis of biologicalmore » lignin upgrading to coproducts in biorefineries will guide the implementation of lignin valorization by mitigating technical risk for scale-up and improving the profitability of biorefinery. Through improving the understanding of biological lignin valorization, it should be possible to create biological lignin valorization route to effectively produce value-added products from lignin.« less

Authors:
 [1];  [2];  [3];  [4];  [1]; ORCiD logo [5]
  1. Texas A & M Univ., College Station, TX (United States)
  2. Univ. of Tennessee, Knoxville, TN (United States)
  3. Fortress Advanced Bioproducts Inc., Vancouver, BC (Canada)
  4. Washington State Univ., Richland, WA (United States)
  5. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1507846
Grant/Contract Number:  
AC05-00OR22725; EE0006112
Resource Type:
Accepted Manuscript
Journal Name:
Renewable and Sustainable Energy Reviews
Additional Journal Information:
Journal Volume: 105; Journal Issue: C; Journal ID: ISSN 1364-0321
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Biological lignin valorization; Ligninolytic microorganism; Bioprospecting; Metabolic engineering; Systems biology; Synthetic biology; Techno-economic analysis

Citation Formats

Liu, Zhi-Hua, Le, Rosemary K., Kosa, Matyas, Yang, Bin, Yuan, Joshua, and Ragauskas, Arthur J. Identifying and creating pathways to improve biological lignin valorization. United States: N. p., 2019. Web. doi:10.1016/j.rser.2019.02.009.
Liu, Zhi-Hua, Le, Rosemary K., Kosa, Matyas, Yang, Bin, Yuan, Joshua, & Ragauskas, Arthur J. Identifying and creating pathways to improve biological lignin valorization. United States. doi:10.1016/j.rser.2019.02.009.
Liu, Zhi-Hua, Le, Rosemary K., Kosa, Matyas, Yang, Bin, Yuan, Joshua, and Ragauskas, Arthur J. Sat . "Identifying and creating pathways to improve biological lignin valorization". United States. doi:10.1016/j.rser.2019.02.009.
@article{osti_1507846,
title = {Identifying and creating pathways to improve biological lignin valorization},
author = {Liu, Zhi-Hua and Le, Rosemary K. and Kosa, Matyas and Yang, Bin and Yuan, Joshua and Ragauskas, Arthur J.},
abstractNote = {Biological lignin valorization to fuels and value-added chemicals enables sustainable and economic biorefineries. Even though significant progress has been made, several major challenges arose due to high recalcitrance and heterogeneity of lignin, which needs to be addressed to improve lignin processing. This work demonstrates an overview of biological lignin conversion and its regulation from a metabolic engineering and systems biology viewpoint. Biological lignin valorization includes three stages: lignin depolymerization, aromatics degradation, and target product biosynthesis. Ligninolytic microorganisms have an extensive enzymatic toolbox to break down the lignin and convert heterogeneous lignin derivatives to central intermediates, such as protocatechuate or catechol, through a peripheral pathway. These intermediates undergo ring cleavage via the β-ketoadipate pathway and are ultimately transformed into metabolites by yielding acetyl-CoA for internal product biosynthesis, such as triacylglycerols, polyhydroxyalkanoates, etc. Bioprospecting will expand the knowledge base of ligninolytic microbial communities, strains, and enzymes to facilitate the understanding of aromatics metabolism. Systems biology analyses achieve an understanding of molecular and systems-level degradation mechanisms and metabolic pathways of lignin and aromatics. By identifying these mechanisms, synthetic biology provides promising approaches to create the lignin conversion pathways and engineer ligninolytic strains suitable as potential hosts for lignin conversion. Techno-economic analysis of biological lignin upgrading to coproducts in biorefineries will guide the implementation of lignin valorization by mitigating technical risk for scale-up and improving the profitability of biorefinery. Through improving the understanding of biological lignin valorization, it should be possible to create biological lignin valorization route to effectively produce value-added products from lignin.},
doi = {10.1016/j.rser.2019.02.009},
journal = {Renewable and Sustainable Energy Reviews},
number = C,
volume = 105,
place = {United States},
year = {2019},
month = {2}
}

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This content will become publicly available on February 16, 2020
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