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Title: Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies

Abstract

We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).

Authors:
 [1];  [2];  [3]; ORCiD logo [3]; ORCiD logo [3]; ORCiD logo [1]
  1. Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany). Dept. of Biomolecular Systems; Freie Univ., Berlin (Germany). Inst. of Chemistry and Biochemistry
  2. Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany). Dept. of Biomolecular Systems
  3. Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
Publication Date:
Research Org.:
Univ. of Georgia, Athens, GA (United States); Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF); German Research Foundation (DFG); Max Planck Society (Germany)
OSTI Identifier:
1507521
Grant/Contract Number:  
FG02-93ER20097; FG05-93ER20097; DBI-0421683; IOS-0923992; PF850/1-1
Resource Type:
Accepted Manuscript
Journal Name:
Organic and Biomolecular Chemistry
Additional Journal Information:
Journal Volume: 15; Journal Issue: 47; Journal ID: ISSN 1477-0520
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Dallabernardina, Pietro, Ruprecht, Colin, Smith, Peter J., Hahn, Michael G., Urbanowicz, Breeanna R., and Pfrengle, Fabian. Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies. United States: N. p., 2017. Web. doi:10.1039/c7ob02605f.
Dallabernardina, Pietro, Ruprecht, Colin, Smith, Peter J., Hahn, Michael G., Urbanowicz, Breeanna R., & Pfrengle, Fabian. Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies. United States. doi:10.1039/c7ob02605f.
Dallabernardina, Pietro, Ruprecht, Colin, Smith, Peter J., Hahn, Michael G., Urbanowicz, Breeanna R., and Pfrengle, Fabian. Tue . "Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies". United States. doi:10.1039/c7ob02605f. https://www.osti.gov/servlets/purl/1507521.
@article{osti_1507521,
title = {Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies},
author = {Dallabernardina, Pietro and Ruprecht, Colin and Smith, Peter J. and Hahn, Michael G. and Urbanowicz, Breeanna R. and Pfrengle, Fabian},
abstractNote = {We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).},
doi = {10.1039/c7ob02605f},
journal = {Organic and Biomolecular Chemistry},
number = 47,
volume = 15,
place = {United States},
year = {2017},
month = {11}
}

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Cited by: 11 works
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Works referenced in this record:

A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies
journal, April 2010

  • Pattathil, S.; Avci, U.; Baldwin, D.
  • Plant Physiology, Vol. 153, Issue 2, p. 514-525
  • DOI: 10.1104/pp.109.151985