Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies

Dallabernardina, Pietro; Ruprecht, Colin; Smith, Peter J.; Hahn, Michael G.; Urbanowicz, Breeanna R.; Pfrengle, Fabian

doi:10.1039/c7ob02605f

Title: Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies

Journal Article · 21 November 2017 · Organic and Biomolecular Chemistry

DOI: https://doi.org/10.1039/c7ob02605f · OSTI ID:1507521

Dallabernardina, Pietro ^[1]; Ruprecht, Colin ^[2]; Smith, Peter J. ^[3];

^[3];

^[1]

Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany). Dept. of Biomolecular Systems; Freie Univ., Berlin (Germany). Inst. of Chemistry and Biochemistry
Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany). Dept. of Biomolecular Systems
Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).

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Research Organization:: Univ. of Georgia, Athens, GA (United States); Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); German Research Foundation (DFG); Max Planck Society (Germany)

Grant/Contract Number:: FG02-93ER20097; FG05-93ER20097; DBI-0421683; IOS-0923992; PF850/1-1

OSTI ID:: 1507521

Journal Information:: Organic and Biomolecular Chemistry, Vol. 15, Issue 47; ISSN 1477-0520

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 26 works

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