Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies

Dallabernardina, Pietro; Ruprecht, Colin; Smith, Peter J.; Hahn, Michael G.; Urbanowicz, Breeanna R.; Pfrengle, Fabian

doi:10.1039/c7ob02605f

Title: Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies

Journal Article · 21 November 2017 · Organic and Biomolecular Chemistry

DOI: https://doi.org/10.1039/c7ob02605f · OSTI ID:1507521

Dallabernardina, Pietro ^[1]; Ruprecht, Colin ^[2]; Smith, Peter J. ^[3];

^[3];

^[4]

Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany). Dept. of Biomolecular Systems; Freie Univ., Berlin (Germany). Inst. of Chemistry and Biochemistry; Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany
Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany). Dept. of Biomolecular Systems
Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany). Dept. of Biomolecular Systems; Freie Univ., Berlin (Germany). Inst. of Chemistry and Biochemistry

We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).

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Research Organization:: Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany); Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: German Research Foundation (DFG); Max Planck Society (Germany); National Science Foundation (NSF) (United States); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: FG02-93ER20097; FG05-93ER20097

OSTI ID:: 1507521

Journal Information:: Organic and Biomolecular Chemistry, Journal Name: Organic and Biomolecular Chemistry Journal Issue: 47 Vol. 15; ISSN 1477-0520

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Analyzing Xyloglucan Endotransglycosylases by Incorporating Synthetic Oligosaccharides into Plant Cell Walls Ruprecht, Colin; Dallabernardina, Pietro; Smith, Peter J. ChemBioChem, Vol. 19, Issue 8 https://doi.org/10.1002/cbic.201700638	journal	February 2018
Automated Glycan Assembly of Plant Oligosaccharides and Their Application in Cell‐Wall Biology Bartetzko, Max P.; Pfrengle, Fabian ChemBioChem, Vol. 20, Issue 7 https://doi.org/10.1002/cbic.201800641	journal	January 2019
Desirable plant cell wall traits for higher-quality miscanthus lignocellulosic biomass da Costa, Ricardo M. F.; Pattathil, Sivakumar; Avci, Utku Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1426-7	journal	April 2019

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