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Title: GUN1 interacts with MORF2 to regulate plastid RNA editing during retrograde signaling

Abstract

During development or under stress, chloroplasts generate signals that regulate the expression of a large number of nuclear genes, a process called retrograde signaling. GENOMES UNCOUPLED 1 (GUN1) is an important regulator of this pathway. In this study, we have discovered an unexpected role for GUN1 in plastid RNA editing, as gun1 mutations affect RNA-editing efficiency at multiple sites in plastids during retrograde signaling. GUN1 plays a direct role in RNA editing by physically interacting with MULTIPLE ORGANELLAR RNA EDITING FACTOR 2 (MORF2). MORF2 overexpression causes widespread RNA-editing changes and a strong genomes uncoupled ( gun ) molecular phenotype similar to gun1 . MORF2 further interacts with RNA-editing site-specificity factors: ORGANELLE TRANSCRIPT PROCESSING 81 (OTP81), ORGANELLE TRANSCRIPT PROCESSING 84 (OTP84), and YELLOW SEEDLINGS 1 (YS1). We further show that otp81 , otp84 , and ys1 single mutants each exhibit a very weak gun phenotype, but combining the three mutations enhances the phenotype. Our study uncovers a role for GUN1 in the regulation of RNA-editing efficiency in damaged chloroplasts and suggests that MORF2 is involved in retrograde signaling.

Authors:
; ;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1507237
Grant/Contract Number:  
FG02-04ER15540
Resource Type:
Published Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America; Journal ID: ISSN 0027-8424
Publisher:
Proceedings of the National Academy of Sciences
Country of Publication:
United States
Language:
English

Citation Formats

Zhao, Xiaobo, Huang, Jianyan, and Chory, Joanne. GUN1 interacts with MORF2 to regulate plastid RNA editing during retrograde signaling. United States: N. p., 2019. Web. doi:10.1073/pnas.1820426116.
Zhao, Xiaobo, Huang, Jianyan, & Chory, Joanne. GUN1 interacts with MORF2 to regulate plastid RNA editing during retrograde signaling. United States. doi:10.1073/pnas.1820426116.
Zhao, Xiaobo, Huang, Jianyan, and Chory, Joanne. Mon . "GUN1 interacts with MORF2 to regulate plastid RNA editing during retrograde signaling". United States. doi:10.1073/pnas.1820426116.
@article{osti_1507237,
title = {GUN1 interacts with MORF2 to regulate plastid RNA editing during retrograde signaling},
author = {Zhao, Xiaobo and Huang, Jianyan and Chory, Joanne},
abstractNote = {During development or under stress, chloroplasts generate signals that regulate the expression of a large number of nuclear genes, a process called retrograde signaling. GENOMES UNCOUPLED 1 (GUN1) is an important regulator of this pathway. In this study, we have discovered an unexpected role for GUN1 in plastid RNA editing, as gun1 mutations affect RNA-editing efficiency at multiple sites in plastids during retrograde signaling. GUN1 plays a direct role in RNA editing by physically interacting with MULTIPLE ORGANELLAR RNA EDITING FACTOR 2 (MORF2). MORF2 overexpression causes widespread RNA-editing changes and a strong genomes uncoupled ( gun ) molecular phenotype similar to gun1 . MORF2 further interacts with RNA-editing site-specificity factors: ORGANELLE TRANSCRIPT PROCESSING 81 (OTP81), ORGANELLE TRANSCRIPT PROCESSING 84 (OTP84), and YELLOW SEEDLINGS 1 (YS1). We further show that otp81 , otp84 , and ys1 single mutants each exhibit a very weak gun phenotype, but combining the three mutations enhances the phenotype. Our study uncovers a role for GUN1 in the regulation of RNA-editing efficiency in damaged chloroplasts and suggests that MORF2 is involved in retrograde signaling.},
doi = {10.1073/pnas.1820426116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {4}
}

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This content will become publicly available on October 15, 2019
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