CAD 1 and CCR 2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

Yan, Xiaojing; Liu, Jie; Kim, Hoon; Liu, Baoguang; Huang, Xiong; Yang, Zhichang; Lin, Ying‐Chung Jimmy; Chen, Hao; Yang, Chenmin; Wang, Jack P.; Muddiman, David C.; Ralph, John; Sederoff, Ronald R.; Li, Quanzi; Chiang, Vincent L.

doi:10.1111/nph.15505

Title: CAD 1 and CCR 2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

Journal Article · 01 October 2018 · New Phytologist

DOI: https://doi.org/10.1111/nph.15505 · OSTI ID:1506674

Yan, Xiaojing ^[1]; Liu, Jie ^[2]; Kim, Hoon ^[3]; Liu, Baoguang ^[4]; Huang, Xiong ^[5]; Yang, Zhichang ^[2]; Lin, Ying‐Chung Jimmy ^[6]; Chen, Hao ^[2]; Yang, Chenmin ^[2]; Wang, Jack P. ^[7]; Muddiman, David C. ^[2]; Ralph, John ^[3]; Sederoff, Ronald R. ^[2]; Li, Quanzi ^[5]; Chiang, Vincent L. ^[7]

Chinese Academy of Forestry, Beijing (China); State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry
North Carolina State Univ., Raleigh, NC (United States)
Univ. of Wisconsin, Madison, WI (United States)
Northeast Forestry Univ., Harbin (China); Beihua Univ., Jilin (China)
Chinese Academy of Forestry, Beijing (China)
Northeast Forestry Univ., Harbin (China); National Taiwan Univ., Taipei (Taiwan)
North Carolina State Univ., Raleigh, NC (United States); Northeast Forestry Univ., Harbin (China)

Lignin is the major phenolic polymer in plant secondary cell walls and is polymerized from monomeric subunits, the monolignols. Eleven enzyme families are implicated in monolignol biosynthesis. In this report, we examined the functions of members of the cinnamyl alcohol dehydrogenase (CAD) and cinnamoyl-CoA reductase (CCR) families in wood formation in Populus trichocarpa, including the regulatory effects of their transcripts and protein activities on monolignol biosynthesis. Enzyme activity assays from stem-differentiating xylem (SDX) proteins showed that RNAi suppression of PtrCAD1 in P. trichocarpa transgenics caused a reduction in SDX CCR activity. RNAi suppression of PtrCCR2, the only CCR member highly expressed in SDX, caused a reciprocal reduction in SDX protein CAD activities. The enzyme assays of mixed and coexpressed recombinant proteins supported physical interactions between PtrCAD1 and PtrCCR2. Biomolecular fluorescence complementation and pull-down/co-immunoprecipitation experiments supported a hypothesis of PtrCAD1/PtrCCR2 heterodimer formation. These results imply evidence for the formation of PtrCAD1/PtrCCR2 protein complexes in monolignol biosynthesis in planta.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: SC0018409

OSTI ID:: 1506674

Journal Information:: New Phytologist, Journal Name: New Phytologist Journal Issue: 1 Vol. 222; ISSN 0028-646X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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co‐immunoprecipitation
enzyme activity
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nuclear magnetic resonance (NMR)
stem‐differentiating xylem protein

Title: CAD 1 and CCR 2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

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