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Title: Insights Into the Mineralogy and Surface Chemistry of Extracellular Biogenic S0 Globules Produced by Chlorobaculum tepidum

Abstract

Elemental sulfur (S0) is produced and degraded by phylogenetically diverse groups of microorganisms. For Chlorobaculum tepidum, an anoxygenic phototroph, sulfide is oxidized to produce extracellular S0 globules, which can be further oxidized to sulfate. While some sulfur-oxidizing bacteria (e.g., Allochromatium vinosum) are also capable of growth on commercial S0 as an electron donor, C. tepidum is not. Even colloidal sulfur sols, which appear indistinguishable from biogenic globules, do not support the growth of C. tepidum. Here, we investigate the properties that make biogenic S0 globules distinct from abiotic forms of S0. We found that S0 globules produced by C. tepidum and abiotic S0 sols are quite similar in terms of mineralogy and material properties, but the two are distinguished primarily by the properties of their surfaces. C. tepidum’s globules are enveloped by a layer of organics (protein and polysaccharides), which results in a surface that is fundamentally different from that of abiotic S0 sols. The organic coating on the globules appears to slow the aging and crystallization of amorphous sulfur, perhaps providing an extended window of time for microbes in the environment to access the more labile forms of sulfur as needed. Overall, our results suggest that the surface ofmore » biogenic S0 globules may be key to cell–sulfur interactions and the reactivity of biogenic S0 in the environment.« less

Authors:
 [1];  [2];  [2];  [2];  [3];  [4];  [5];  [6];  [5]
  1. Niagara Univ., Lewiston, NY (United States); Univ. of Delaware, Newark, DE (United States)
  2. Delaware Biotechnology Inst., Newark, DE (United States)
  3. Univ. of Delaware, Newark, DE (United States)
  4. Carnegie Inst. of Science, Washington, DC (United States)
  5. Delaware Biotechnology Inst., Newark, DE (United States); Univ. of Delaware, Newark, DE (United States)
  6. SLAC National Accelerator Lab., Menlo Park, CA (United States)
Publication Date:
Research Org.:
SLAC National Accelerator Lab.(SLAC), Menlo Park, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1506230
Grant/Contract Number:  
AC02-76SF00515; INBRE220RR016472-09; MCB-1244373; EPS-0814251
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 10; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; elemental sulfur; biomineralization; sulfur cycling; green sulfur bacteria; microbe-mineral interactions

Citation Formats

Marnocha, Cassandra L., Sabanayagam, Chandran R., Modla, Shannon, Powell, Deborah H., Henri, Pauline A., Steele, Andrew S., Hanson, Thomas E., Webb, Samuel M., and Chan, Clara S. Insights Into the Mineralogy and Surface Chemistry of Extracellular Biogenic S0 Globules Produced by Chlorobaculum tepidum. United States: N. p., 2019. Web. doi:10.3389/fmicb.2019.00271.
Marnocha, Cassandra L., Sabanayagam, Chandran R., Modla, Shannon, Powell, Deborah H., Henri, Pauline A., Steele, Andrew S., Hanson, Thomas E., Webb, Samuel M., & Chan, Clara S. Insights Into the Mineralogy and Surface Chemistry of Extracellular Biogenic S0 Globules Produced by Chlorobaculum tepidum. United States. doi:10.3389/fmicb.2019.00271.
Marnocha, Cassandra L., Sabanayagam, Chandran R., Modla, Shannon, Powell, Deborah H., Henri, Pauline A., Steele, Andrew S., Hanson, Thomas E., Webb, Samuel M., and Chan, Clara S. Mon . "Insights Into the Mineralogy and Surface Chemistry of Extracellular Biogenic S0 Globules Produced by Chlorobaculum tepidum". United States. doi:10.3389/fmicb.2019.00271. https://www.osti.gov/servlets/purl/1506230.
@article{osti_1506230,
title = {Insights Into the Mineralogy and Surface Chemistry of Extracellular Biogenic S0 Globules Produced by Chlorobaculum tepidum},
author = {Marnocha, Cassandra L. and Sabanayagam, Chandran R. and Modla, Shannon and Powell, Deborah H. and Henri, Pauline A. and Steele, Andrew S. and Hanson, Thomas E. and Webb, Samuel M. and Chan, Clara S.},
abstractNote = {Elemental sulfur (S0) is produced and degraded by phylogenetically diverse groups of microorganisms. For Chlorobaculum tepidum, an anoxygenic phototroph, sulfide is oxidized to produce extracellular S0 globules, which can be further oxidized to sulfate. While some sulfur-oxidizing bacteria (e.g., Allochromatium vinosum) are also capable of growth on commercial S0 as an electron donor, C. tepidum is not. Even colloidal sulfur sols, which appear indistinguishable from biogenic globules, do not support the growth of C. tepidum. Here, we investigate the properties that make biogenic S0 globules distinct from abiotic forms of S0. We found that S0 globules produced by C. tepidum and abiotic S0 sols are quite similar in terms of mineralogy and material properties, but the two are distinguished primarily by the properties of their surfaces. C. tepidum’s globules are enveloped by a layer of organics (protein and polysaccharides), which results in a surface that is fundamentally different from that of abiotic S0 sols. The organic coating on the globules appears to slow the aging and crystallization of amorphous sulfur, perhaps providing an extended window of time for microbes in the environment to access the more labile forms of sulfur as needed. Overall, our results suggest that the surface of biogenic S0 globules may be key to cell–sulfur interactions and the reactivity of biogenic S0 in the environment.},
doi = {10.3389/fmicb.2019.00271},
journal = {Frontiers in Microbiology},
number = ,
volume = 10,
place = {United States},
year = {2019},
month = {2}
}

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Figures / Tables:

Figure 1 Figure 1: Chlorobaculum tepidum biogenic S0 globules (Ct S0) compared to a standard of bulk crystalline $\alpha$-S8. (A) XRD patterns, with peak broadening in S0 globules. (B) Raman spectra.

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