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Title: Crystal structure of the SH3 domain of human Lyn non-receptor tyrosine kinase

Abstract

Lyn kinase (Lck/Yes related novel protein tyrosine kinase) belongs to the family of Src-related non-receptor tyrosine kinases. Consistent with physiological roles in cell growth and proliferation, aberrant function of Lyn is associated with various forms of cancer, including leukemia, breast cancer and melanoma. Here, we determine a 1.3 Å resolution crystal structure of the polyproline-binding SH3 regulatory domain of human Lyn kinase, which adopts a five-stranded β-barrel fold. Mapping of cancer-associated point mutations onto this structure reveals that these amino acid substitutions are distributed throughout the SH3 domain and may affect Lyn kinase function distinctly.

Authors:
ORCiD logo; ; ORCiD logo;
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1506167
Alternate Identifier(s):
OSTI ID: 1627880
Grant/Contract Number:  
AC02-76SF00515; R35GM122491; GM120569; DA043680
Resource Type:
Published Article
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Name: PLoS ONE Journal Volume: 14 Journal Issue: 4; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science (PLoS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Science & Technology - Other Topics

Citation Formats

Berndt, Sandra, Gurevich, Vsevolod V., Iverson, T. M., and Boggon, ed., Titus J. Crystal structure of the SH3 domain of human Lyn non-receptor tyrosine kinase. United States: N. p., 2019. Web. doi:10.1371/journal.pone.0215140.
Berndt, Sandra, Gurevich, Vsevolod V., Iverson, T. M., & Boggon, ed., Titus J. Crystal structure of the SH3 domain of human Lyn non-receptor tyrosine kinase. United States. doi:10.1371/journal.pone.0215140.
Berndt, Sandra, Gurevich, Vsevolod V., Iverson, T. M., and Boggon, ed., Titus J. Wed . "Crystal structure of the SH3 domain of human Lyn non-receptor tyrosine kinase". United States. doi:10.1371/journal.pone.0215140.
@article{osti_1506167,
title = {Crystal structure of the SH3 domain of human Lyn non-receptor tyrosine kinase},
author = {Berndt, Sandra and Gurevich, Vsevolod V. and Iverson, T. M. and Boggon, ed., Titus J.},
abstractNote = {Lyn kinase (Lck/Yes related novel protein tyrosine kinase) belongs to the family of Src-related non-receptor tyrosine kinases. Consistent with physiological roles in cell growth and proliferation, aberrant function of Lyn is associated with various forms of cancer, including leukemia, breast cancer and melanoma. Here, we determine a 1.3 Å resolution crystal structure of the polyproline-binding SH3 regulatory domain of human Lyn kinase, which adopts a five-stranded β-barrel fold. Mapping of cancer-associated point mutations onto this structure reveals that these amino acid substitutions are distributed throughout the SH3 domain and may affect Lyn kinase function distinctly.},
doi = {10.1371/journal.pone.0215140},
journal = {PLoS ONE},
number = 4,
volume = 14,
place = {United States},
year = {2019},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1371/journal.pone.0215140

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