Title: Core Metabolism Shifts during Growth on Methanol versus Methane in the Methanotroph Methylomicrobium buryatense 5GB1

Abstract

One-carbon compounds such as methane and methanol are of increasing interest as sustainable substrates for biological production of fuels and industrial chemicals. The bacteria that carry out these conversions have been studied for many decades, but gaps exist in our knowledge of their metabolic pathways. One such gap is the difference between growth on methane and growth on methanol. Understanding such metabolism is important, since each has advantages and disadvantages as a feedstock for production of chemicals and fuels. The significance of our research is in the demonstration that the metabolic network is substantially altered in each case and in the delineation of these changes. The resulting new insights into the core metabolism of this bacterium now provide an improved basis for future strain design.

Authors:

Fu, Yanfen ^[1]; He, Lian ^[1]; Reeve, Jennifer ^[1]; Beck, David A. C. ^[2]; Lidstrom, Mary E. ^[3]; Cavanaugh, ed., Colleen M.; Murrell, Colin; Wang, Jin

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+ Show Author Affiliations

1. Department of Chemical Engineering, University of Washington, Seattle, Washington, USA
2. Department of Chemical Engineering, University of Washington, Seattle, Washington, USA, eScience Institute, University of Washington, Seattle, Washington, USA
3. Department of Chemical Engineering, University of Washington, Seattle, Washington, USA, Department of Microbiology, University of Washington, Seattle, Washington, USA

Publication Date:

Tue Apr 30 00:00:00 EDT 2019

Research Org.:

Univ. of Washington, Seattle, WA (United States)

Sponsoring Org.:

USDOE Advanced Research Projects Agency - Energy (ARPA-E)

OSTI Identifier:

1505657

Alternate Identifier(s):

OSTI ID: 1904831

Grant/Contract Number:  

AR0000350

Resource Type:

Published Article

Journal Name:

mBio

Additional Journal Information:

Journal Name: mBio Journal Volume: 10 Journal Issue: 2; Journal ID: ISSN 2161-2129

Publisher:

American Society for Microbiology

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; Microbiology; 13C tracer analysis; flux balance analysis; methanol; methanotrophs