Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane
Abstract
Sugarcane, a tropical C4 grass in the genus Saccharum (Poaceae), accounts for nearly 80% of sugar produced worldwide and is also an important feedstock for biofuel production. Generating transgenic sugarcane with predictable and stable transgene expression is critical for crop improvement. We generated a highly expressed single copy locus as landing pad for transgene stacking. Transgenic sugarcane lines with stable integration of a single copy nptII expression cassette flanked by insulators supported higher transgene expression along with reduced line to line variation when compared to single copy events without insulators by NPTII ELISA analysis. Subsequently, the nptII selectable marker gene was efficiently excised from the sugarcane genome by the FLPe/FRT site-specific recombination system to create selectable marker free plants. This study provides valuable resources for future gene stacking using site-specific recombination or genome editing tools.
- Authors:
-
[4]
- Univ. of Florida, Gainesville, FL (United States). Agronomy Dept. Plant Molecular and Cellular Biology Program. Genetics Inst.
- Univ. of Florida, Gainesville, FL (United States). Agronomy Dept. Plant Molecular and Cellular Biology Program. Genetics Inst.; Kongju National Univ., Yesan (Korea, Republic of). Dept. of Plant Resources. College of Industrial Science
- Univ. of Florida, Gainesville, FL (United States). Agronomy Dept. Plant Molecular and Cellular Biology Program. Genetics Inst.; Korea Inst. of Science and Technology (KIST), Gangwon (Korea, Republic of). Smart Farm Research Center. Inst. of Natural Products
- Univ. of Florida, Gainesville, FL (United States). Agronomy Dept. Plant Molecular and Cellular Biology Program. Genetics Inst. DOE Center for Advanced Bioenergy and Bioproducts Innovation
- Syngenta Crop Protection, LLC, Research Triangle Park, NC (United States)
- Univ. of Arkansas, Fayetteville, AR (United States). Crop, Soil and Environmental Sciences
- Publication Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States); Syngenta Crop Protection, LLC, Research Triangle Park, NC (United States); Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1505510
- Grant/Contract Number:
- SC0018420
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Plant Molecular Biology
- Additional Journal Information:
- Journal Volume: 100; Journal Issue: 3; Journal ID: ISSN 0167-4412
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; sugarcane; selection marker removal; site-specific recombination; insulator; FLP; FLPe
Citation Formats
Zhao, Yang, Kim, Jae Y., Karan, Ratna, Jung, Je H., Pathak, Bhuvan, Williamson, Bruce, Kannan, Baskaran, Wang, Duoduo, Fan, Chunyang, Yu, Wenjin, Dong, Shujie, Srivastava, Vibha, and Altpeter, Fredy. Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane. United States: N. p., 2019.
Web. doi:10.1007/s11103-019-00856-4.
Zhao, Yang, Kim, Jae Y., Karan, Ratna, Jung, Je H., Pathak, Bhuvan, Williamson, Bruce, Kannan, Baskaran, Wang, Duoduo, Fan, Chunyang, Yu, Wenjin, Dong, Shujie, Srivastava, Vibha, & Altpeter, Fredy. Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane. United States. doi:10.1007/s11103-019-00856-4.
Zhao, Yang, Kim, Jae Y., Karan, Ratna, Jung, Je H., Pathak, Bhuvan, Williamson, Bruce, Kannan, Baskaran, Wang, Duoduo, Fan, Chunyang, Yu, Wenjin, Dong, Shujie, Srivastava, Vibha, and Altpeter, Fredy. Wed .
"Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane". United States. doi:10.1007/s11103-019-00856-4. https://www.osti.gov/servlets/purl/1505510.
@article{osti_1505510,
title = {Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane},
author = {Zhao, Yang and Kim, Jae Y. and Karan, Ratna and Jung, Je H. and Pathak, Bhuvan and Williamson, Bruce and Kannan, Baskaran and Wang, Duoduo and Fan, Chunyang and Yu, Wenjin and Dong, Shujie and Srivastava, Vibha and Altpeter, Fredy},
abstractNote = {Sugarcane, a tropical C4 grass in the genus Saccharum (Poaceae), accounts for nearly 80% of sugar produced worldwide and is also an important feedstock for biofuel production. Generating transgenic sugarcane with predictable and stable transgene expression is critical for crop improvement. We generated a highly expressed single copy locus as landing pad for transgene stacking. Transgenic sugarcane lines with stable integration of a single copy nptII expression cassette flanked by insulators supported higher transgene expression along with reduced line to line variation when compared to single copy events without insulators by NPTII ELISA analysis. Subsequently, the nptII selectable marker gene was efficiently excised from the sugarcane genome by the FLPe/FRT site-specific recombination system to create selectable marker free plants. This study provides valuable resources for future gene stacking using site-specific recombination or genome editing tools.},
doi = {10.1007/s11103-019-00856-4},
journal = {Plant Molecular Biology},
number = 3,
volume = 100,
place = {United States},
year = {2019},
month = {3}
}
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Works referenced in this record:
Site-specific excisional recombination strategies for elimination of undesirable transgenes from crop plants
journal, June 2008
- Gidoni, David; Srivastava, Vibha; Carmi, Nir
- In Vitro Cellular & Developmental Biology - Plant, Vol. 44, Issue 6
Effect of various growth regulators on shoot regeneration of sugarcane
journal, July 2001
- Chengalrayan, K.; Gallo-Meagher, M.
- In Vitro Cellular & Developmental Biology - Plant, Vol. 37, Issue 4
Transgene expression in rice engineered through particle bombardment: molecular factors controlling stable expression and transgene silencing
journal, March 1999
- Kohli, Ajay; Gahakwa, Daphrose; Vain, Philippe
- Planta, Vol. 208, Issue 1
Rapid production of transgenic sugarcane with the introduction of simple loci following biolistic transfer of a minimal expression cassette and direct embryogenesis
journal, August 2011
- Taparia, Yogesh; Fouad, Walid M.; Gallo, Maria
- In Vitro Cellular & Developmental Biology - Plant, Vol. 48, Issue 1
The Human β Globin Locus Introduced by YAC Transfer Exhibits a Specific and Reproducible Pattern of Developmental Regulation in Transgenic Mice
journal, December 1997
- Porcu, Susanna; Kitamura, Michael; Witkowska, Ewa
- Blood, Vol. 90, Issue 11
Site-specific recombination for genetic engineering in plants
journal, June 2003
- Lyznik, L. A.; Gordon-Kamm, W. J.; Tao, Y.
- Plant Cell Reports, Vol. 21, Issue 10
Simultaneous excision of two transgene flanking sequences and resolution of complex integration loci
journal, September 2008
- Kumar, Sandeep; Thompson, William F.
- Plant Molecular Biology, Vol. 69, Issue 1-2
Variegated transgene expression in mouse mammary gland is determined by the transgene integration locus.
journal, June 1996
- Dobie, K. W.; Lee, M.; Fantes, J. A.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 13
TALEN-mediated targeted mutagenesis of more than 100 COMT copies/alleles in highly polyploid sugarcane improves saccharification efficiency without compromising biomass yield
journal, November 2017
- Kannan, Baskaran; Jung, Je Hyeong; Moxley, Geoffrey W.
- Plant Biotechnology Journal, Vol. 16, Issue 4
High-frequency, precise modification of the tomato genome
journal, November 2015
- Čermák, Tomáš; Baltes, Nicholas J.; Čegan, Radim
- Genome Biology, Vol. 16, Issue 1
Functionality of the β/six Site-Specific Recombination System in Tobacco and Arabidopsis: A Novel Tool for Genetic Engineering of Plant Genomes
journal, November 2006
- Grønlund, Jesper T.; Stemmer, Christian; Lichota, Jacek
- Plant Molecular Biology, Vol. 63, Issue 4
Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco.
journal, August 1997
- Iglesias, V. A.; Moscone, E. A.; Papp, I.
- The Plant Cell, Vol. 9, Issue 8
When more is better: multigene engineering in plants
journal, January 2010
- Naqvi, Shaista; Farré, Gemma; Sanahuja, Georgina
- Trends in Plant Science, Vol. 15, Issue 1
A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques
journal, November 2004
- Travella, S.; Ross, S. M.; Harden, J.
- Plant Cell Reports, Vol. 23, Issue 12
Enhancer–promoter interference and its prevention in transgenic plants
journal, December 2010
- Singer, Stacy D.; Cox, Kerik D.; Liu, Zongrang
- Plant Cell Reports, Vol. 30, Issue 5
Homology-Dependent gene Silencing in Plants
journal, June 1996
- Meyer, P.; Saedler, H.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 47, Issue 1
Cre-mediated site-specific gene integration for consistent transgene expression in rice: Cre-mediated site-specific gene integration
journal, February 2004
- Srivastava, Vibha; Ariza-Nieto, Magnolia; Wilson, Andrea J.
- Plant Biotechnology Journal, Vol. 2, Issue 2
Effect of stacking insecticidal cry and herbicide tolerance epsps transgenes on transgenic maize proteome
journal, December 2014
- Agapito-Tenfen, Sarah Zanon; Vilperte, Vinicius; Benevenuto, Rafael Fonseca
- BMC Plant Biology, Vol. 14, Issue 1
Application of pyramided traits against Lepidoptera in insect resistance management for Bt crops
journal, July 2012
- Storer, Nicholas P.; Thompson, Gary D.; Head, Graham P.
- GM Crops & Food, Vol. 3, Issue 3
ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome
journal, August 2008
- Thomson, James G.; Yau, Yuan-Yeu; Blanvillain, Robert
- Transgenic Research, Vol. 18, Issue 2
FLP-mediated site-specific recombination for genome modification in turfgrass
journal, August 2006
- Hu, Qian; Nelson, Kimberly; Luo, Hong
- Biotechnology Letters, Vol. 28, Issue 22
Posttranscriptional Gene Silencing in Transgenic Sugarcane. Dissection of Homology-Dependent Virus Resistance in a Monocot That Has a Complex Polyploid Genome
journal, April 1999
- Ingelbrecht, Ivan L.; Irvine, James E.; Mirkov, T. Erik
- Plant Physiology, Vol. 119, Issue 4
Co-integration, co-expression and inheritance of unlinked minimal transgene expression cassettes in an apomictic turf and forage grass (Paspalum notatum Flugge)
journal, August 2008
- Sandhu, Sukhpreet; Altpeter, Fredy
- Plant Cell Reports, Vol. 27, Issue 11
A mosaic monoploid reference sequence for the highly complex genome of sugarcane
journal, July 2018
- Garsmeur, Olivier; Droc, Gaetan; Antonise, Rudie
- Nature Communications, Vol. 9, Issue 1
Quantitative Real-Time PCR Assay for Determining Transgene Copy Number in Transformed Plants
journal, July 2001
- Ingham, David J.; Beer, Sandra; Money, Stephanie
- BioTechniques, Vol. 31, Issue 1
PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome
journal, January 2010
- Thomson, James G.; Chan, Ronald; Thilmony, Roger
- BMC Biotechnology, Vol. 10, Issue 1
Post-Transcriptional Gene Silencing in Cultured Rice Cells
journal, March 2000
- Kanno, T.; Naito, S.; Shimamoto, K.
- Plant and Cell Physiology, Vol. 41, Issue 3
Rapid isolation of high molecular weight plant DNA
journal, January 1980
- Murray, M. G.; Thompson, W. F.
- Nucleic Acids Research, Vol. 8, Issue 19
Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize
journal, November 2008
- Zhu, C.; Naqvi, S.; Breitenbach, J.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 47
Chromosomal position effects and the modulation of transgene expression
journal, January 1994
- Clark, Aj; Bissinger, P.; Bullock, Dw
- Reproduction, Fertility and Development, Vol. 6, Issue 5
Gene stacking by recombinases
journal, August 2015
- Srivastava, Vibha; Thomson, James
- Plant Biotechnology Journal, Vol. 14, Issue 2
To be or not to be active: the stochastic nature of enhancer action
journal, March 2000
- Fiering, Steve; Whitelaw, Emma; Martin, David I. K.
- BioEssays, Vol. 22, Issue 4
An Open-Source System for In Planta Gene Stacking by Bxb1 and Cre Recombinases
journal, December 2014
- Hou, Lili; Yau, Yuan-Yeu; Wei, Junjie
- Molecular Plant, Vol. 7, Issue 12
Transcription Activator-Like Effector Nucleases (TALEN)-Mediated Targeted DNA Insertion in Potato Plants
journal, October 2016
- Forsyth, Adrienne; Weeks, Troy; Richael, Craig
- Frontiers in Plant Science, Vol. 7
Site-specific gene integration technologies for crop improvement
journal, February 2010
- Srivastava, Vibha; Gidoni, David
- In Vitro Cellular & Developmental Biology - Plant, Vol. 46, Issue 3
Improved FLP Recombinase, FLPe, Efficiently Removes Marker Gene from Transgene Locus Developed by Cre–lox Mediated Site-Specific Gene Integration in Rice
journal, January 2011
- Akbudak, M. Aydın; Srivastava, Vibha
- Molecular Biotechnology, Vol. 49, Issue 1
TALEN mediated targeted mutagenesis of the caffeic acid O-methyltransferase in highly polyploid sugarcane improves cell wall composition for production of bioethanol
journal, June 2016
- Jung, Je Hyeong; Altpeter, Fredy
- Plant Molecular Biology, Vol. 92, Issue 1-2
Site-specific gene integration in rice genome mediated by the FLP-FRT recombination system: FLP-FRT-mediated gene integration in plants
journal, November 2010
- Nandy, Soumen; Srivastava, Vibha
- Plant Biotechnology Journal, Vol. 9, Issue 6
Auto-excision of selectable marker genes from transgenic tobacco via a stress inducible FLP/FRT site-specific recombination system
journal, January 2009
- Woo, Hee-Jong; Cho, Hyun-Suk; Lim, Sun-Hyung
- Transgenic Research, Vol. 18, Issue 3
High-throughput Agrobacterium-mediated barley transformation
journal, January 2008
- Bartlett, Joanne G.; Alves, Sílvia C.; Smedley, Mark
- Plant Methods, Vol. 4, Issue 1
FLP recombinase-mediated site-specific recombination in rice
journal, February 2008
- Hu, Qian; Kononowicz-Hodges, Halina; Nelson-Vasilchik, Kimberly
- Plant Biotechnology Journal, Vol. 6, Issue 2
Genetic Transformation of Wheat Mediated by Agrobacterium tumefaciens
journal, November 1997
- Cheng, M.; Fry, J. E.; Pang, S.
- Plant Physiology, Vol. 115, Issue 3
A novel approach to plastid transformation utilizes the phiC31 phage integrase
journal, March 2004
- Lutz, Kerry A.; Corneille, Sylvie; Azhagiri, Arun K.
- The Plant Journal, Vol. 37, Issue 6
Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences
journal, August 1996
- Jorgensen, Richard A.; Cluster, Paul D.; English, James
- Plant Molecular Biology, Vol. 31, Issue 5
Biotechnological Prospects for Engineering Insect-Resistant Plants
journal, March 2008
- Gatehouse, John A.
- Plant Physiology, Vol. 146, Issue 3
Assessment of transgenic maize events produced by particle bombardment or Agrobacterium-mediated transformation
journal, February 2004
- Shou, Huixia; Frame, Bronwyn R.; Whitham, Steven A.
- Molecular Breeding, Vol. 13, Issue 2
A 5′ element of the chicken β-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila
journal, August 1993
- Chung, Jay H.; Whiteley, Mary; Felsenfeld, Gary
- Cell, Vol. 74, Issue 3
Strong activity of FLPe recombinase in rice plants does not correlate with the transmission of the recombined locus to the progeny
journal, September 2014
- Nguyen, Linh D.; Underwood, Jamie L.; Nandy, Soumen
- Plant Biotechnology Reports, Vol. 8, Issue 6
Transgenic mice containing a 248-kb yeast artificial chromosome carrying the human beta-globin locus display proper developmental control of human globin genes.
journal, August 1993
- Peterson, K. R.; Clegg, C. H.; Huxley, C.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 16
Metabolically engineered oilseed crops with enhanced seed tocopherol
journal, September 2005
- Karunanandaa, Balasulojini; Qi, Qungang; Hao, Ming
- Metabolic Engineering, Vol. 7, Issue 5-6
Transgene copy number can be positively or negatively associated with transgene expression
journal, January 1993
- Hobbs, Shaun L. A.; Warkentin, Thomas D.; DeLong, Catherine M. O.
- Plant Molecular Biology, Vol. 21, Issue 1
T-DNA insertional mutagenesis for functional genomics in rice
journal, June 2000
- Jeon, Jong-Seong; Lee, Sichul; Jung, Ki-Hong
- The Plant Journal, Vol. 22, Issue 6
Targeted integration and removal of transgenes in hybrid aspen (Populus tremula L. x P. tremuloides Michx.) using site-specific recombination systems: Targeted integration and removal of transgenes in hybrid aspen
journal, December 2009
- Fladung, M.; Becker, D.
- Plant Biology, Vol. 12, Issue 2
Gene silencing as a threat to the success of gene therapy
journal, February 2000
- Bestor, Timothy H.
- Journal of Clinical Investigation, Vol. 105, Issue 4
Transformation Systems for Generating Marker–Free Transgenic Plants
journal, March 1994
- Yoder, John I.; Goldsbrough, Andrew P.
- Bio/Technology, Vol. 12, Issue 3
The Potential Benefits of Insulators on Heterologous Constructs in Transgenic Animals
journal, December 2003
- Giraldo, Patricia; Rival-Gervier, Sylvie; Houdebine, Louis-Marie
- Transgenic Research, Vol. 12, Issue 6
Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators
journal, October 2011
- Singer, Stacy D.; Liu, Zongrang; Cox, Kerik D.
- Plant Cell Reports, Vol. 31, Issue 1
A 1-kb Bacteriophage Lambda Fragment Functions as an Insulator to Effectively Block Enhancer–Promoter Interactions in Arabidopsis thaliana
journal, August 2009
- Singer, Stacy D.; Hily, Jean-Michel; Liu, Zongrang
- Plant Molecular Biology Reporter, Vol. 28, Issue 1
Post-transcriptional silencing of chalcone synthase in Petunia by inverted transgene repeats
journal, July 1997
- Stam, Maike; de Bruin, Rob; Kenter, Susan
- The Plant Journal, Vol. 12, Issue 1
Evaluation and comparison of the insulation efficiency of three enhancer-blocking insulators in plants
journal, November 2010
- Yang, Yazhou; Singer, Stacy D.; Liu, Zongrang
- Plant Cell, Tissue and Organ Culture (PCTOC), Vol. 105, Issue 3
Enhanced single copy integration events in corn via particle bombardment using low quantities of DNA
journal, April 2009
- Lowe, Brenda A.; Shiva Prakash, N.; Way, Melissa
- Transgenic Research, Vol. 18, Issue 6
Functional analysis of the HS185 regulatory element in the rice HSP70 promoter
journal, June 2011
- Zhang, Yu-Man; Zheng, Yu-Mei; Xiao, Na
- Molecular Biology Reports, Vol. 39, Issue 2
A transformation booster sequence (TBS) from Petunia hybrida functions as an enhancer-blocking insulator in Arabidopsis thaliana
journal, April 2009
- Hily, Jean-Michel; Singer, Stacy D.; Yang, Yazhou
- Plant Cell Reports, Vol. 28, Issue 7
Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA
journal, June 1997
- Angell, S. M.
- The EMBO Journal, Vol. 16, Issue 12
Gene stacking in transgenic plants - the challenge for 21st century plant biotechnology: The challenge of transgene and trait stacking
journal, February 2005
- Halpin, Claire
- Plant Biotechnology Journal, Vol. 3, Issue 2
Transgene expression produced by biolistic-mediated, site-specific gene integration is consistently inherited by the subsequent generations
journal, March 2006
- Chawla, Rekha; Ariza-Nieto, Magnolia; Wilson, Andrea J.
- Plant Biotechnology Journal, Vol. 4, Issue 2
Evaluation of Bt Corn with Pyramided Genes on Efficacy and Insect Resistance Management for the Asian Corn Borer in China
journal, December 2016
- Jiang, Fan; Zhang, Tiantao; Bai, Shuxiong
- PLOS ONE, Vol. 11, Issue 12
Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass
journal, June 2015
- Zale, Janice; Jung, Je Hyeong; Kim, Jae Yoon
- Plant Biotechnology Journal, Vol. 14, Issue 2
Epigenetic repeat-induced gene silencing (RIGS) inArabidopsis
journal, September 1993
- Assaad, Farhah F.; Tucker, Kerry Lee; Signer, Ethan R.
- Plant Molecular Biology, Vol. 22, Issue 6
Insulators: many functions, many mechanisms
journal, February 2002
- West, A. G.
- Genes & Development, Vol. 16, Issue 3
Improved properties of FLP recombinase evolved by cycling mutagenesis
journal, July 1998
- Buchholz, Frank; Angrand, Pierre-Olivier; Stewart, A. Francis
- Nature Biotechnology, Vol. 16, Issue 7
Position-independent, high-level expression of the human β-globin gene in transgenic mice
journal, December 1987
- Grosveld, Frank; van Assendelft, Greet Blom; Greaves, David R.
- Cell, Vol. 51, Issue 6
DNA methylation and chromatin modification
journal, April 1999
- Ng, Huck-Hui; Adrian, Bird
- Current Opinion in Genetics & Development, Vol. 9, Issue 2
Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms
journal, July 2001
- Moellenbeck, Daniel J.; Peters, Melvin L.; Bing, James W.
- Nature Biotechnology, Vol. 19, Issue 7
Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome
journal, April 1995
- Albert, Henrik; Dale, Emily C.; Lee, Elsa
- The Plant Journal, Vol. 7, Issue 4, p. 649-659
Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium-mediated transformation
journal, September 2006
- Wu, Huixia; Sparks, Caroline A.; Jones, Huw D.
- Molecular Breeding, Vol. 18, Issue 3
Transgene integration complexity and expression stability following biolistic or Agrobacterium-mediated transformation of sugarcane
journal, August 2015
- Wu, Hao; Awan, Faisal Saeed; Vilarinho, Aloisio
- In Vitro Cellular & Developmental Biology - Plant, Vol. 51, Issue 6
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
journal, May 1976
- Bradford, Marion M.
- Analytical Biochemistry, Vol. 72, Issue 1-2
Post-transcriptional gene silencing in plants
journal, June 1997
- Depicker, Ann; Van Montagu, Marc
- Current Opinion in Cell Biology, Vol. 9, Issue 3
Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation.
journal, October 1994
- Ingelbrecht, I.; Van Houdt, H.; Van Montagu, M.
- Proceedings of the National Academy of Sciences, Vol. 91, Issue 22
Generation of Single-Copy T-DNA Transformants in Arabidopsis by the CRE/ loxP Recombination-Mediated Resolution System
journal, August 2007
- De Buck, Sylvie; Peck, Ingrid; De Wilde, Chris
- Plant Physiology, Vol. 145, Issue 4
Site-Specific Integration of Transgenes in Soybean via Recombinase-Mediated DNA Cassette Exchange
journal, May 2009
- Li, Z.; Xing, A.; Moon, B. P.
- Plant Physiology, Vol. 151, Issue 3, p. 1087-1095
T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives
journal, May 1987
- Jorgensen, Richard; Snyder, Christine; Jones, Jonathan D. G.
- MGG Molecular & General Genetics, Vol. 207, Issue 2-3
Comparison of direct and indirect embryogenesis protocols, biolistic gene transfer and selection parameters for efficient genetic transformation of sugarcane
journal, May 2012
- Taparia, Yogesh; Gallo, Maria; Altpeter, Fredy
- Plant Cell, Tissue and Organ Culture (PCTOC), Vol. 111, Issue 2
Site-specific T-DNA integration in Arabidopsis thaliana mediated by the combined action of CRE recombinase and ϕC31 integrase
journal, May 2013
- De Paepe, Annelies; De Buck, Sylvie; Nolf, Jonah
- The Plant Journal, Vol. 75, Issue 1
The Agrobacterium tumefaciens virulence D2 protein is responsible for precise integration of T-DNA into the plant genome.
journal, July 1995
- Tinland, B.; Schoumacher, F.; Gloeckler, V.
- The EMBO Journal, Vol. 14, Issue 14
Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas.
journal, June 1997
- Cerutti, H.; Johnson, A. M.; Gillham, N. W.
- The Plant Cell, Vol. 9, Issue 6
Somatic and germinal inheritance of an FLP-mediated deletion in transgenic tobacco
journal, September 1999
- Davies, G. J.; Kilby, N. J.; Riou-Khamlichi, C.
- Journal of Experimental Botany, Vol. 50, Issue 338
Comparison of Agrobacterium and particle bombardment using whole plasmid or minimal cassette for production of high-expressing, low-copy transgenic plants
journal, August 2012
- Jackson, Mark A.; Anderson, David J.; Birch, Robert G.
- Transgenic Research, Vol. 22, Issue 1