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Title: Experimental 3D coherent diffractive imaging from photon-sparse random projections

Abstract

The routine atomic resolution structure determination of single particles is expected to have profound implications for probing structure–function relationships in systems ranging from energy-storage materials to biological molecules. Extremely bright ultrashort-pulse X-ray sources – X-ray free-electron lasers (XFELs) – provide X-rays that can be used to probe ensembles of nearly identical nanoscale particles. When combined with coherent diffractive imaging, these objects can be imaged; however, as the resolution of the images approaches the atomic scale, the measured data are increasingly difficult to obtain and, during an X-ray pulse, the number of photons incident on the 2D detector is much smaller than the number of pixels. This latter concern, the signal `sparsity', materially impedes the application of the method. An experimental analog using a conventional X-ray source is demonstrated and yields signal levels comparable with those expected from single biomolecules illuminated by focused XFEL pulses. The analog experiment provides an invaluable cross check on the fidelity of the reconstructed data that is not available during XFEL experiments. Using these experimental data, it is established that a sparsity of order 1.3 × 10 -3 photons per pixel per frame can be overcome, lending vital insight to the solution of the atomic resolutionmore » XFEL single-particle imaging problem by experimentally demonstrating 3D coherent diffractive imaging from photon-sparse random projections.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [5];  [5];  [5];  [6];  [6];  [6];  [3];  [7];  [4];  [8];  [9];  [8];  [10];  [5];  [11]
  1. European XFEL GmbH, Schenefeld (Germany)
  2. European XFEL GmbH, Schenefeld (Germany); SLAC National Accelerator Lab., Menlo Park, CA (United States)
  3. National Univ. of Singapore (Singapore)
  4. ESRF – The European Synchrotron, Grenoble (France)
  5. Cornell Univ., Ithaca, NY (United States)
  6. European XFEL GmbH, Schenefeld (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  7. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  8. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  9. Stanford Univ., Stanford, CA (United States)
  10. SLAC National Accelerator Lab., Menlo Park, CA (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)
  11. European XFEL GmbH, Schenefeld (Germany); La Trobe Univ., Melbourne, VIC (Australia)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1504880
Report Number(s):
BNL-211503-2019-JAAM
Journal ID: ISSN 2052-2525; IUCRAJ
Grant/Contract Number:  
SC0012704
Resource Type:
Accepted Manuscript
Journal Name:
IUCrJ
Additional Journal Information:
Journal Volume: 6; Journal Issue: 3; Journal ID: ISSN 2052-2525
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; coherent X-ray diffractive imaging (CXDI); X-ray free-electron lasers; XFELs; phase problem; single particles

Citation Formats

Giewekemeyer, Klaus, Aquila, A., Loh, N. -T. D., Chushkin, Y., Shanks, K. S., Weiss, J. T., Tate, M. W., Philipp, H. T., Stern, S., Vagovic, P., Mehrjoo, M., Teo, C., Barthelmess, M., Zontone, F., Chang, C., Tiberio, R. C., Sakdinawat, A., Williams, Garth J., Gruner, S. M., and Mancuso, A. P. Experimental 3D coherent diffractive imaging from photon-sparse random projections. United States: N. p., 2019. Web. doi:10.1107/S2052252519002781.
Giewekemeyer, Klaus, Aquila, A., Loh, N. -T. D., Chushkin, Y., Shanks, K. S., Weiss, J. T., Tate, M. W., Philipp, H. T., Stern, S., Vagovic, P., Mehrjoo, M., Teo, C., Barthelmess, M., Zontone, F., Chang, C., Tiberio, R. C., Sakdinawat, A., Williams, Garth J., Gruner, S. M., & Mancuso, A. P. Experimental 3D coherent diffractive imaging from photon-sparse random projections. United States. doi:10.1107/S2052252519002781.
Giewekemeyer, Klaus, Aquila, A., Loh, N. -T. D., Chushkin, Y., Shanks, K. S., Weiss, J. T., Tate, M. W., Philipp, H. T., Stern, S., Vagovic, P., Mehrjoo, M., Teo, C., Barthelmess, M., Zontone, F., Chang, C., Tiberio, R. C., Sakdinawat, A., Williams, Garth J., Gruner, S. M., and Mancuso, A. P. Wed . "Experimental 3D coherent diffractive imaging from photon-sparse random projections". United States. doi:10.1107/S2052252519002781. https://www.osti.gov/servlets/purl/1504880.
@article{osti_1504880,
title = {Experimental 3D coherent diffractive imaging from photon-sparse random projections},
author = {Giewekemeyer, Klaus and Aquila, A. and Loh, N. -T. D. and Chushkin, Y. and Shanks, K. S. and Weiss, J. T. and Tate, M. W. and Philipp, H. T. and Stern, S. and Vagovic, P. and Mehrjoo, M. and Teo, C. and Barthelmess, M. and Zontone, F. and Chang, C. and Tiberio, R. C. and Sakdinawat, A. and Williams, Garth J. and Gruner, S. M. and Mancuso, A. P.},
abstractNote = {The routine atomic resolution structure determination of single particles is expected to have profound implications for probing structure–function relationships in systems ranging from energy-storage materials to biological molecules. Extremely bright ultrashort-pulse X-ray sources – X-ray free-electron lasers (XFELs) – provide X-rays that can be used to probe ensembles of nearly identical nanoscale particles. When combined with coherent diffractive imaging, these objects can be imaged; however, as the resolution of the images approaches the atomic scale, the measured data are increasingly difficult to obtain and, during an X-ray pulse, the number of photons incident on the 2D detector is much smaller than the number of pixels. This latter concern, the signal `sparsity', materially impedes the application of the method. An experimental analog using a conventional X-ray source is demonstrated and yields signal levels comparable with those expected from single biomolecules illuminated by focused XFEL pulses. The analog experiment provides an invaluable cross check on the fidelity of the reconstructed data that is not available during XFEL experiments. Using these experimental data, it is established that a sparsity of order 1.3 × 10-3 photons per pixel per frame can be overcome, lending vital insight to the solution of the atomic resolution XFEL single-particle imaging problem by experimentally demonstrating 3D coherent diffractive imaging from photon-sparse random projections.},
doi = {10.1107/S2052252519002781},
journal = {IUCrJ},
number = 3,
volume = 6,
place = {United States},
year = {2019},
month = {3}
}

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Works referenced in this record:

Femtosecond X-ray protein nanocrystallography
journal, February 2011

  • Chapman, Henry N.; Fromme, Petra; Barty, Anton
  • Nature, Vol. 470, Issue 7332, p. 73-77
  • DOI: 10.1038/nature09750

Solving protein structure from sparse serial microcrystal diffraction data at a storage-ring synchrotron source
journal, July 2018


Serial femtosecond crystallography: the first five years
journal, February 2015


Single-particle imaging without symmetry constraints at an X-ray free-electron laser
journal, September 2018


Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses
journal, October 2017

  • Kurta, Ruslan P.; Donatelli, Jeffrey J.; Yoon, Chun Hong
  • Physical Review Letters, Vol. 119, Issue 15, Article No. 158102
  • DOI: 10.1103/PhysRevLett.119.158102

Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
journal, April 2017