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Title: Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery

Agave-based alcoholic beverage companies generate thousands of tons of solid residues per year in Mexico. These agave residues might be used for biofuel production due to their abundance and favorable sustainability characteristics. In this work, agave leaf and bagasse residues from species Agave tequilana and Agave salmiana were subjected to pretreatment using the ammonia fiber expansion (AFEX) process. The pretreatment conditions were optimized using a response surface design methodology. We also identified commercial enzyme mixtures that maximize sugar yields for AFEX-pretreated agave bagasse and leaf matter, at ~ 6% glucan (w/w) loading enzymatic hydrolysis. Finally, the pretreated agave hydrolysates (at a total solids loading of ~ 20%) were used for ethanol fermentation using the glucose- and xylose-consuming strain Saccharomyces cerevisiae 424A (LNH-ST), to determine ethanol yields at industrially relevant conditions.
Authors:
ORCiD logo [1] ;  [1] ;  [2] ;  [3] ;  [3] ; ORCiD logo [4]
  1. Tecnologico Nacional de Mexico, Guanajuato (Mexico)
  2. Tianjin Univ. of Science & Technology, Tianjin (People's Republic of China)
  3. Michigan State Univ., East Lansing, MI (United States)
  4. Michigan State Univ., East Lansing, MI (United States); Univ. of Houston, Houston, TX (United States)
Publication Date:
Grant/Contract Number:
FC02-07ER64494
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Agave; Biomass; Pretreatment; AFEX; Enzymatic hydrolysis; Cellulase; Fermentation; Biofuel; Lignocellulosic; Ethanol
OSTI Identifier:
1504761

Flores-Gómez, Carlos A., Escamilla Silva, Eleazar M., Zhong, Cheng, Dale, Bruce E., da Costa Sousa, Leonardo, and Balan, Venkatesh. Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery. United States: N. p., Web. doi:10.1186/s13068-017-0995-6.
Flores-Gómez, Carlos A., Escamilla Silva, Eleazar M., Zhong, Cheng, Dale, Bruce E., da Costa Sousa, Leonardo, & Balan, Venkatesh. Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery. United States. doi:10.1186/s13068-017-0995-6.
Flores-Gómez, Carlos A., Escamilla Silva, Eleazar M., Zhong, Cheng, Dale, Bruce E., da Costa Sousa, Leonardo, and Balan, Venkatesh. 2018. "Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery". United States. doi:10.1186/s13068-017-0995-6. https://www.osti.gov/servlets/purl/1504761.
@article{osti_1504761,
title = {Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery},
author = {Flores-Gómez, Carlos A. and Escamilla Silva, Eleazar M. and Zhong, Cheng and Dale, Bruce E. and da Costa Sousa, Leonardo and Balan, Venkatesh},
abstractNote = {Agave-based alcoholic beverage companies generate thousands of tons of solid residues per year in Mexico. These agave residues might be used for biofuel production due to their abundance and favorable sustainability characteristics. In this work, agave leaf and bagasse residues from species Agave tequilana and Agave salmiana were subjected to pretreatment using the ammonia fiber expansion (AFEX) process. The pretreatment conditions were optimized using a response surface design methodology. We also identified commercial enzyme mixtures that maximize sugar yields for AFEX-pretreated agave bagasse and leaf matter, at ~ 6% glucan (w/w) loading enzymatic hydrolysis. Finally, the pretreated agave hydrolysates (at a total solids loading of ~ 20%) were used for ethanol fermentation using the glucose- and xylose-consuming strain Saccharomyces cerevisiae 424A (LNH-ST), to determine ethanol yields at industrially relevant conditions.},
doi = {10.1186/s13068-017-0995-6},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 11,
place = {United States},
year = {2018},
month = {1}
}

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book, January 2009

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
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Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification
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Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
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Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review
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