skip to main content

DOE PAGESDOE PAGES

Title: Anthropogenic N Deposition Alters the Composition of Expressed Class II Fungal Peroxidases

Here, we present evidence that ca. 20 years of experimental N deposition altered the composition of lignin-decaying class II peroxidases expressed by forest floor fungi, a response which has occurred concurrently with reductions in plant litter decomposition and a rapid accumulation of soil organic matter. This finding suggests that anthropogenic N deposition has induced changes in the biological mediation of lignin decay, the rate limiting step in plant litter decomposition. Hence, an altered composition of transcripts for a critical gene that is associated with terrestrial C cycling may explain the increased soil C storage under long-term increases in anthropogenic N deposition. IMPORTANCE: Fungal class II peroxidases are enzymes that mediate the rate-limiting step in the decomposition of plant material, which involves the oxidation of lignin and other polyphenols. In field experiments, anthropogenic N deposition has increased soil C storage in forests, a result which could potentially arise from anthropogenic N-induced changes in the composition of class II peroxidases expressed by the fungal community. For this study, we have gained unique insight into how anthropogenic N deposition, a widespread agent of global change, affects the expression of a functional gene encoding an enzyme that plays a critical role in a biologicallymore » mediated ecosystem process.« less
Authors:
 [1] ;  [2] ;  [2] ; ORCiD logo [3] ;  [4] ;  [2] ;  [5]
  1. Univ. of Michigan, Ann Arbor, MI (United States); Univ. of Alabama, Birmingham, AL (United States). Dept. of Biology
  2. Univ. of Michigan, Ann Arbor, MI (United States)
  3. West Virginia Univ., Morgantown, WV (United States)
  4. Univ. of Alabama, Birmingham, AL (United States). Dept. of Biology
  5. McMaster Univ., Hamilton, ON (Canada)
Publication Date:
Grant/Contract Number:
SC0010541
Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 84; Journal Issue: 9; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Research Org:
Univ. of Michigan, Ann Arbor, MI (United States); Univ. of California, Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; fungal peroxidase; fungi; peroxidase; atmospheric N deposition; peroxidases; soil carbon
OSTI Identifier:
1503614

Entwistle, Elizabeth M., Romanowicz, Karl J., Argiroff, William A., Freedman, Zachary B., Morris, J. Jeffrey, Zak, Donald R., and Elliot, Marie A.. Anthropogenic N Deposition Alters the Composition of Expressed Class II Fungal Peroxidases. United States: N. p., Web. doi:10.1128/aem.02816-17.
Entwistle, Elizabeth M., Romanowicz, Karl J., Argiroff, William A., Freedman, Zachary B., Morris, J. Jeffrey, Zak, Donald R., & Elliot, Marie A.. Anthropogenic N Deposition Alters the Composition of Expressed Class II Fungal Peroxidases. United States. doi:10.1128/aem.02816-17.
Entwistle, Elizabeth M., Romanowicz, Karl J., Argiroff, William A., Freedman, Zachary B., Morris, J. Jeffrey, Zak, Donald R., and Elliot, Marie A.. 2018. "Anthropogenic N Deposition Alters the Composition of Expressed Class II Fungal Peroxidases". United States. doi:10.1128/aem.02816-17. https://www.osti.gov/servlets/purl/1503614.
@article{osti_1503614,
title = {Anthropogenic N Deposition Alters the Composition of Expressed Class II Fungal Peroxidases},
author = {Entwistle, Elizabeth M. and Romanowicz, Karl J. and Argiroff, William A. and Freedman, Zachary B. and Morris, J. Jeffrey and Zak, Donald R. and Elliot, Marie A.},
abstractNote = {Here, we present evidence that ca. 20 years of experimental N deposition altered the composition of lignin-decaying class II peroxidases expressed by forest floor fungi, a response which has occurred concurrently with reductions in plant litter decomposition and a rapid accumulation of soil organic matter. This finding suggests that anthropogenic N deposition has induced changes in the biological mediation of lignin decay, the rate limiting step in plant litter decomposition. Hence, an altered composition of transcripts for a critical gene that is associated with terrestrial C cycling may explain the increased soil C storage under long-term increases in anthropogenic N deposition. IMPORTANCE: Fungal class II peroxidases are enzymes that mediate the rate-limiting step in the decomposition of plant material, which involves the oxidation of lignin and other polyphenols. In field experiments, anthropogenic N deposition has increased soil C storage in forests, a result which could potentially arise from anthropogenic N-induced changes in the composition of class II peroxidases expressed by the fungal community. For this study, we have gained unique insight into how anthropogenic N deposition, a widespread agent of global change, affects the expression of a functional gene encoding an enzyme that plays a critical role in a biologically mediated ecosystem process.},
doi = {10.1128/aem.02816-17},
journal = {Applied and Environmental Microbiology},
number = 9,
volume = 84,
place = {United States},
year = {2018},
month = {2}
}

Works referenced in this record:

Review: lignin conversion by manganese peroxidase (MnP)
journal, April 2002

KEGG: Kyoto Encyclopedia of Genes and Genomes
journal, January 2000
  • Kanehisa, Minoru; Goto, Susumu
  • Nucleic Acids Research, Vol. 28, Issue 1, p. 27-30
  • DOI: 10.1093/nar/28.1.27

Enzymatic "Combustion": The Microbial Degradation of Lignin
journal, October 1987

Pathways for degradation of lignin in bacteria and fungi
journal, January 2011
  • Bugg, Timothy D. H.; Ahmad, Mark; Hardiman, Elizabeth M.
  • Natural Product Reports, Vol. 28, Issue 12, p. 1883-1896
  • DOI: 10.1039/c1np00042j

Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
journal, October 2009
  • Schloss, P. D.; Westcott, S. L.; Ryabin, T.
  • Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541
  • DOI: 10.1128/AEM.01541-09

MUSCLE: multiple sequence alignment with high accuracy and high throughput
journal, March 2004
  • Edgar, R. C.
  • Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797
  • DOI: 10.1093/nar/gkh340