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Title: Codominant grasses differ in gene expression under experimental climate extremes in native tallgrass prairie

Abstract

Extremes in climate, such as heat waves and drought, are expected to become more frequent and intense with forecasted climate change. Plant species will almost certainly differ in their responses to these stressors. We experimentally imposed a heat wave and drought in the tallgrass prairie ecosystem near Manhattan, Kansas, USA to assess transcriptional responses of two ecologically important C 4 grass species, Andropogon gerardii and Sorghastrum nutans. Based on previous research, we expected that S. nutans would regulate more genes, particularly those related to stress response, under high heat and drought. Across all treatments, S. nutans showed greater expression of negative regulatory and catabolism genes while A. gerardii upregulated cellular and protein metabolism. As predicted, S. nutans showed greater sensitivity to water stress, particularly with downregulation of non-coding RNAs and upregulation of water stress and catabolism genes. A. gerardii was less sensitive to drought, although A. gerardii tended to respond with upregulation in response to drought versus S. nutans which downregulated more genes under drier conditions. Surprisingly, A. gerardii only showed minimal gene expression response to increased temperature, while S. nutans showed no response. Gene functional annotation suggested that these two species may respond to stress via different mechanisms. Specifically,more » A. gerardii tends to maintain molecular function while S. nutans prioritizes avoidance. Sorghastrum nutans may strategize abscisic acid response and catabolism to respond rapidly to stress. Here these results have important implications for success of these two important grass species under a more variable and extreme climate forecast for the future.« less

Authors:
 [1];  [2];  [1];  [1]
  1. Colorado State Univ., Fort Collins, CO (United States)
  2. The Johns Hopkins Univ., Baltimore, MD (United States)
Publication Date:
Research Org.:
Yale Univ., New Haven, CT (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Climate and Environmental Sciences Division
OSTI Identifier:
1503611
Grant/Contract Number:  
FG02-04ER63892
Resource Type:
Accepted Manuscript
Journal Name:
PeerJ
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2167-8359
Publisher:
PeerJ Inc.
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Andropogon gerardii; Sorghastrum nutans; Microarray; Drought; Heat wave; C4 grass; Dominant species

Citation Formats

Hoffman, Ava M., Avolio, Meghan L., Knapp, Alan K., and Smith, Melinda D.. Codominant grasses differ in gene expression under experimental climate extremes in native tallgrass prairie. United States: N. p., 2018. Web. doi:10.7717/peerj.4394.
Hoffman, Ava M., Avolio, Meghan L., Knapp, Alan K., & Smith, Melinda D.. Codominant grasses differ in gene expression under experimental climate extremes in native tallgrass prairie. United States. doi:10.7717/peerj.4394.
Hoffman, Ava M., Avolio, Meghan L., Knapp, Alan K., and Smith, Melinda D.. Wed . "Codominant grasses differ in gene expression under experimental climate extremes in native tallgrass prairie". United States. doi:10.7717/peerj.4394. https://www.osti.gov/servlets/purl/1503611.
@article{osti_1503611,
title = {Codominant grasses differ in gene expression under experimental climate extremes in native tallgrass prairie},
author = {Hoffman, Ava M. and Avolio, Meghan L. and Knapp, Alan K. and Smith, Melinda D.},
abstractNote = {Extremes in climate, such as heat waves and drought, are expected to become more frequent and intense with forecasted climate change. Plant species will almost certainly differ in their responses to these stressors. We experimentally imposed a heat wave and drought in the tallgrass prairie ecosystem near Manhattan, Kansas, USA to assess transcriptional responses of two ecologically important C4 grass species, Andropogon gerardii and Sorghastrum nutans. Based on previous research, we expected that S. nutans would regulate more genes, particularly those related to stress response, under high heat and drought. Across all treatments, S. nutans showed greater expression of negative regulatory and catabolism genes while A. gerardii upregulated cellular and protein metabolism. As predicted, S. nutans showed greater sensitivity to water stress, particularly with downregulation of non-coding RNAs and upregulation of water stress and catabolism genes. A. gerardii was less sensitive to drought, although A. gerardii tended to respond with upregulation in response to drought versus S. nutans which downregulated more genes under drier conditions. Surprisingly, A. gerardii only showed minimal gene expression response to increased temperature, while S. nutans showed no response. Gene functional annotation suggested that these two species may respond to stress via different mechanisms. Specifically, A. gerardii tends to maintain molecular function while S. nutans prioritizes avoidance. Sorghastrum nutans may strategize abscisic acid response and catabolism to respond rapidly to stress. Here these results have important implications for success of these two important grass species under a more variable and extreme climate forecast for the future.},
doi = {10.7717/peerj.4394},
journal = {PeerJ},
number = ,
volume = 6,
place = {United States},
year = {2018},
month = {2}
}

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