Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes
Abstract
Here, the enigma of how a metaphase chromosome is constructed remains unclear with the higher order structure still under investigation. It is clear that this is a 3D structural problem requiring the development of new technologies necessary to answer this long standing mystery. This mini-review highlights new and recent, advanced 3D imaging methods that have been used to image mitotic human chromosomes. These 3D methods have advantages and limitations that are both discussed, Furthermore chromosome sample preparation and future imaging directions are also highlighted.
- Authors:
-
[1];
[3]
- Univ. College London, London (United Kingdom); Aga Khan Univ., Karachi (Pakistan)
- Osaka Univ., Osaka (Japan)
- Univ. College London, London (United Kingdom); Brookhaven National Lab. (BNL), Upton, NY (United States)
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1503225
- Alternate Identifier(s):
- OSTI ID: 1462414
- Report Number(s):
- BNL-211436-2019-JAAM; BNL-207897-2018-JAAM
Journal ID: ISSN 0009-5915
- Grant/Contract Number:
- SC0012704
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Chromosoma
- Additional Journal Information:
- Journal Volume: 128; Journal Issue: 1; Journal ID: ISSN 0009-5915
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; Chromosome structure; Chromatin; 3D; High-resolution imaging
Citation Formats
Yusuf, Mohammed, Kaneyoshi, Kohei, Fukui, Kiichi, and Robinson, Ian. Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes. United States: N. p., 2018.
Web. doi:10.1007/s00412-018-0678-5.
Yusuf, Mohammed, Kaneyoshi, Kohei, Fukui, Kiichi, & Robinson, Ian. Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes. United States. https://doi.org/10.1007/s00412-018-0678-5
Yusuf, Mohammed, Kaneyoshi, Kohei, Fukui, Kiichi, and Robinson, Ian. Mon .
"Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes". United States. https://doi.org/10.1007/s00412-018-0678-5. https://www.osti.gov/servlets/purl/1503225.
@article{osti_1503225,
title = {Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes},
author = {Yusuf, Mohammed and Kaneyoshi, Kohei and Fukui, Kiichi and Robinson, Ian},
abstractNote = {Here, the enigma of how a metaphase chromosome is constructed remains unclear with the higher order structure still under investigation. It is clear that this is a 3D structural problem requiring the development of new technologies necessary to answer this long standing mystery. This mini-review highlights new and recent, advanced 3D imaging methods that have been used to image mitotic human chromosomes. These 3D methods have advantages and limitations that are both discussed, Furthermore chromosome sample preparation and future imaging directions are also highlighted.},
doi = {10.1007/s00412-018-0678-5},
journal = {Chromosoma},
number = 1,
volume = 128,
place = {United States},
year = {Mon Sep 03 00:00:00 EDT 2018},
month = {Mon Sep 03 00:00:00 EDT 2018}
}
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