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Title: Phytobiome and Transcriptional Adaptation of Populus deltoides to Acute Progressive Drought and Cyclic Drought

Abstract

Plant drought stress causes systematic changes to photosynthesis, metabolism, growth, and potentially the phytobiome. Additionally, drought affects plants in both a species-specific and water-deficit-driven manner, causing the response to drought to be dependent both on how drought is being experienced and on any adaptation to prior drought exposure. Thus, understanding the effect of drought on plants requires assessing drought response in multiple conditions, such as progressive acute drought and recurrent cyclic drought, and at different levels of severity. In this study, we have utilized RNA sequencing to identify changes to the plant transcriptome and the phytobiome during both acute progressive drought and cyclic drought at multiple severities. Co-analysis of the plant and phytobiome, utilizing the same RNAseq data, allows for the identification of novel associations that would not be possible otherwise. We have identified that the drought response ranges from increased transcripts related to photosynthesis and metabolic activity in mild acute drought to decreased transcripts related to photosynthesis and metabolic impairment in severe drought. Moreover, while water deficit is a main driver of transcriptional responses in severe drought, there are increases in reactive oxygen species (ROS) metabolism and photosynthetic transcripts in cyclic severe drought compared with acute severe drought, independentmore » of water deficit. The phytobiome exhibits alternate responses to drought when compared with the transcriptome. Specifically, the phytobiome is affected more by the cyclic or acute nature of the drought rather than the severity of the drought, with the phytobiome having an increase in taxa under cyclic drought that are often reported to have beneficial effects on the plants. Finally, we have identified associations between taxa in the phytobiome with expression of disease response, ROS metabolism, and photosynthesis transcripts suggesting interplay between the host plant and its phytobiome in response to drought.« less

Authors:
ORCiD logo [1]; ORCiD logo [1];  [2]; ORCiD logo [1];  [3];  [4];  [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [2]
+ Show Author Affiliations
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Stanford Univ., Stanford, CA (United States)
  4. Univ. of Missouri, St. Louis, MO (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1502604
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Phytobiomes Journal
Additional Journal Information:
Journal Volume: 2; Journal Issue: 4; Journal ID: ISSN 2471-2906
Publisher:
American Phytopathological Society (APS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bacteriology; microbiome; mutualism; mycology; nematology; nutrient cycling; populus deltoides; rhizosphere and phyllosphere; symbiosis; transcriptomics

Citation Formats

Garcia, Benjamin J., Labbé, Jessy L., Jones, Piet C., Abraham, Paul E., Hodge, Ian, Climer, Sharlee, Jawdy, Sara, Gunter, Lee E., Tuskan, Gerald A., Yang, Xiaohan, Tschaplinski, Timothy J., and Jacobson, Daniel A. Phytobiome and Transcriptional Adaptation of Populus deltoides to Acute Progressive Drought and Cyclic Drought. United States: N. p., 2018. Web. doi:10.1094/PBIOMES-04-18-0021-R.
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Garcia, Benjamin J., Labbé, Jessy L., Jones, Piet C., Abraham, Paul E., Hodge, Ian, Climer, Sharlee, Jawdy, Sara, Gunter, Lee E., Tuskan, Gerald A., Yang, Xiaohan, Tschaplinski, Timothy J., & Jacobson, Daniel A. Phytobiome and Transcriptional Adaptation of Populus deltoides to Acute Progressive Drought and Cyclic Drought. United States. https://doi.org/10.1094/PBIOMES-04-18-0021-R
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Garcia, Benjamin J., Labbé, Jessy L., Jones, Piet C., Abraham, Paul E., Hodge, Ian, Climer, Sharlee, Jawdy, Sara, Gunter, Lee E., Tuskan, Gerald A., Yang, Xiaohan, Tschaplinski, Timothy J., and Jacobson, Daniel A. Thu . "Phytobiome and Transcriptional Adaptation of Populus deltoides to Acute Progressive Drought and Cyclic Drought". United States. https://doi.org/10.1094/PBIOMES-04-18-0021-R. https://www.osti.gov/servlets/purl/1502604.
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@article{osti_1502604,
title = {Phytobiome and Transcriptional Adaptation of Populus deltoides to Acute Progressive Drought and Cyclic Drought},
author = {Garcia, Benjamin J. and Labbé, Jessy L. and Jones, Piet C. and Abraham, Paul E. and Hodge, Ian and Climer, Sharlee and Jawdy, Sara and Gunter, Lee E. and Tuskan, Gerald A. and Yang, Xiaohan and Tschaplinski, Timothy J. and Jacobson, Daniel A.},
abstractNote = {Plant drought stress causes systematic changes to photosynthesis, metabolism, growth, and potentially the phytobiome. Additionally, drought affects plants in both a species-specific and water-deficit-driven manner, causing the response to drought to be dependent both on how drought is being experienced and on any adaptation to prior drought exposure. Thus, understanding the effect of drought on plants requires assessing drought response in multiple conditions, such as progressive acute drought and recurrent cyclic drought, and at different levels of severity. In this study, we have utilized RNA sequencing to identify changes to the plant transcriptome and the phytobiome during both acute progressive drought and cyclic drought at multiple severities. Co-analysis of the plant and phytobiome, utilizing the same RNAseq data, allows for the identification of novel associations that would not be possible otherwise. We have identified that the drought response ranges from increased transcripts related to photosynthesis and metabolic activity in mild acute drought to decreased transcripts related to photosynthesis and metabolic impairment in severe drought. Moreover, while water deficit is a main driver of transcriptional responses in severe drought, there are increases in reactive oxygen species (ROS) metabolism and photosynthetic transcripts in cyclic severe drought compared with acute severe drought, independent of water deficit. The phytobiome exhibits alternate responses to drought when compared with the transcriptome. Specifically, the phytobiome is affected more by the cyclic or acute nature of the drought rather than the severity of the drought, with the phytobiome having an increase in taxa under cyclic drought that are often reported to have beneficial effects on the plants. Finally, we have identified associations between taxa in the phytobiome with expression of disease response, ROS metabolism, and photosynthesis transcripts suggesting interplay between the host plant and its phytobiome in response to drought.},
doi = {10.1094/PBIOMES-04-18-0021-R},
journal = {Phytobiomes Journal},
number = 4,
volume = 2,
place = {United States},
year = {Thu Mar 01 00:00:00 EST 2018},
month = {Thu Mar 01 00:00:00 EST 2018}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1094/PBIOMES-04-18-0021-R
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Cited by: 13 works
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Figures / Tables:

Fig. 1 Fig. 1: Drought experimental design. Plants were exposed to two different drought conditions: acute drought (one drought exposure) and cyclic drought (four drought and three rewatering cycles). RNAseq was performed on fully expandedmature leaves (~10 leaves from the apex) in both drought and control samples. Both the host transcriptome andmore » phytobiome taxa were profiled and analyzed for differential expression/ abundance and pairwise associations.« less
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Works referencing / citing this record:

Differences in resource use lead to coexistence of seed-transmitted microbial populations
journal, April 2019

Plant Host-Associated Mechanisms for Microbial Selection
journal, July 2019

Advances in Omics Approaches for Abiotic Stress Tolerance in Tomato
journal, November 2019

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