Microscopy with ultraviolet surface excitation (MUSE) enables translation of optical biopsy principles to enhance life science education
Abstract
The translation of microscopy with ultraviolet surface excitation (MUSE) into a high school science classroom is investigated with the goal of providing a suitable new modality to enhance life science education. A key part of this effort is the development of laboratory exercises that can integrate the advanced capabilities of MUSE into a classroom setting. MUSE utilizes the unique property of ultraviolet light at wavelengths between 250 and 285 nm to propagate about 10 um into tissues, thus illuminating only the top cell layer. Furthermore this illumination is provided by a low-power UV LED source, which enables one to cost-efficiently implement this method into the educational environment. MUSE in education can eliminate the need for premade microscope slides and provide a far more engaging and rewarding experience for students.
- Authors:
-
- Univ. of Rochester, Rochester, NY (United States)
- Publication Date:
- Research Org.:
- Univ. of Rochester, NY (United States). Lab. for Laser Energetics
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- Contributing Org.:
- Laboratory for Laser Energetics, University of Rochester
- OSTI Identifier:
- 1502408
- Report Number(s):
- 2018-268, 1479
Journal ID: ISSN 0277-786X; 2018-268, 1479, 2438
- Grant/Contract Number:
- NA0003856
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Proceedings of SPIE - The International Society for Optical Engineering
- Additional Journal Information:
- Journal Volume: 10873; Conference: Photonics West, San Francisco, CA (United States), 2-7 Feb 2019; Journal ID: ISSN 0277-786X
- Publisher:
- SPIE
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; fluorescence microscope; ultraviolet fluorescence microscopy; curriculum; education; MUSE microscopy
Citation Formats
Kopp, Katherine A., Demos, Stavros G., and Kosc, Tanya Z. Microscopy with ultraviolet surface excitation (MUSE) enables translation of optical biopsy principles to enhance life science education. United States: N. p., 2019.
Web. doi:10.1117/12.2515366.
Kopp, Katherine A., Demos, Stavros G., & Kosc, Tanya Z. Microscopy with ultraviolet surface excitation (MUSE) enables translation of optical biopsy principles to enhance life science education. United States. https://doi.org/10.1117/12.2515366
Kopp, Katherine A., Demos, Stavros G., and Kosc, Tanya Z. Mon .
"Microscopy with ultraviolet surface excitation (MUSE) enables translation of optical biopsy principles to enhance life science education". United States. https://doi.org/10.1117/12.2515366. https://www.osti.gov/servlets/purl/1502408.
@article{osti_1502408,
title = {Microscopy with ultraviolet surface excitation (MUSE) enables translation of optical biopsy principles to enhance life science education},
author = {Kopp, Katherine A. and Demos, Stavros G. and Kosc, Tanya Z.},
abstractNote = {The translation of microscopy with ultraviolet surface excitation (MUSE) into a high school science classroom is investigated with the goal of providing a suitable new modality to enhance life science education. A key part of this effort is the development of laboratory exercises that can integrate the advanced capabilities of MUSE into a classroom setting. MUSE utilizes the unique property of ultraviolet light at wavelengths between 250 and 285 nm to propagate about 10 um into tissues, thus illuminating only the top cell layer. Furthermore this illumination is provided by a low-power UV LED source, which enables one to cost-efficiently implement this method into the educational environment. MUSE in education can eliminate the need for premade microscope slides and provide a far more engaging and rewarding experience for students.},
doi = {10.1117/12.2515366},
journal = {Proceedings of SPIE - The International Society for Optical Engineering},
number = ,
volume = 10873,
place = {United States},
year = {Mon Mar 04 00:00:00 EST 2019},
month = {Mon Mar 04 00:00:00 EST 2019}
}
Works referenced in this record:
Adaptation of microscopy with ultraviolet surface excitation for enhancing STEM and undergraduate education
journal, July 2018
- Huang, Chi Z. R.; Wood, Ronald W.; Demos, Stavros G.
- Journal of Biomedical Optics, Vol. 23, Issue 12
Characterizing the origin of autofluorescence in human esophageal epithelium under ultraviolet excitation
journal, January 2010
- Lin, Bevin; Urayama, Shiro; Saroufeem, Ramez M. G.
- Optics Express, Vol. 18, Issue 20, Article No. 21074
Microscopy with ultraviolet surface excitation for rapid slide-free histology
journal, December 2017
- Fereidouni, Farzad; Harmany, Zachary T.; Tian, Miao
- Nature Biomedical Engineering, Vol. 1, Issue 12