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Title: P450s controlling metabolic bifurcations in plant terpene specialized metabolism

Abstract

Catalyzing stereo- and regio-specific oxidation of inert hydrocarbon backbones, and a range of more exotic reactions inherently difficult in formal chemical synthesis, cytochromes P450 (P450s) offer outstanding potential for biotechnological engineering. Plants and their dazzling diversity of specialized metabolites have emerged as rich repository for functional P450s with the advances of deep transcriptomics and genome wide discovery. P450s are of outstanding interest for understanding chemical diversification throughout evolution, for gaining mechanistic insights through the study of their structure–function relationship, and for exploitation in Synthetic Biology. In this review, we highlight recent developments and examples in the discovery of plant P450s involved in the biosynthesis of industrially relevant monoterpenoids, sesquiterpenoids, diterpenoids and triterpenoids, throughout 2016 and early 2017. Examples were selected to illustrate the spectrum of value from commodity chemicals, flavor and fragrance compounds to pharmacologically active terpenoids. We focus on a recently emerging theme, where P450s control metabolic bifurcations and chemical diversity of the final product profile, either within a pathway, or through neo-functionalization in related species. In conclusion, the implications may inform approaches for rational assembly of recombinant pathways, biotechnological production of high value terpenoids and generation of novel chemical entities.

Authors:
 [1]; ORCiD logo [1]
  1. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1502091
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Accepted Manuscript
Journal Name:
Phytochemistry Reviews
Additional Journal Information:
Journal Volume: 17; Journal Issue: 1; Journal ID: ISSN 1568-7767
Publisher:
Springer Nature
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Terpenoid specialized metabolites; Pathway bifurcation; Regio-specificity; Stereo-specificity; Promiscuity; Orthologs; Biosynthetic; Metabolic diversity; Synthetic Biology; Metabolic engineering; Biotechnology

Citation Formats

Banerjee, Aparajita, and Hamberger, Björn. P450s controlling metabolic bifurcations in plant terpene specialized metabolism. United States: N. p., 2017. Web. doi:10.1007/s11101-017-9530-4.
Banerjee, Aparajita, & Hamberger, Björn. P450s controlling metabolic bifurcations in plant terpene specialized metabolism. United States. doi:10.1007/s11101-017-9530-4.
Banerjee, Aparajita, and Hamberger, Björn. Tue . "P450s controlling metabolic bifurcations in plant terpene specialized metabolism". United States. doi:10.1007/s11101-017-9530-4. https://www.osti.gov/servlets/purl/1502091.
@article{osti_1502091,
title = {P450s controlling metabolic bifurcations in plant terpene specialized metabolism},
author = {Banerjee, Aparajita and Hamberger, Björn},
abstractNote = {Catalyzing stereo- and regio-specific oxidation of inert hydrocarbon backbones, and a range of more exotic reactions inherently difficult in formal chemical synthesis, cytochromes P450 (P450s) offer outstanding potential for biotechnological engineering. Plants and their dazzling diversity of specialized metabolites have emerged as rich repository for functional P450s with the advances of deep transcriptomics and genome wide discovery. P450s are of outstanding interest for understanding chemical diversification throughout evolution, for gaining mechanistic insights through the study of their structure–function relationship, and for exploitation in Synthetic Biology. In this review, we highlight recent developments and examples in the discovery of plant P450s involved in the biosynthesis of industrially relevant monoterpenoids, sesquiterpenoids, diterpenoids and triterpenoids, throughout 2016 and early 2017. Examples were selected to illustrate the spectrum of value from commodity chemicals, flavor and fragrance compounds to pharmacologically active terpenoids. We focus on a recently emerging theme, where P450s control metabolic bifurcations and chemical diversity of the final product profile, either within a pathway, or through neo-functionalization in related species. In conclusion, the implications may inform approaches for rational assembly of recombinant pathways, biotechnological production of high value terpenoids and generation of novel chemical entities.},
doi = {10.1007/s11101-017-9530-4},
journal = {Phytochemistry Reviews},
number = 1,
volume = 17,
place = {United States},
year = {2017},
month = {9}
}

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    Works referencing / citing this record:

    Functional characterization of the cytochrome P450 monooxygenase CYP71AU87 indicates a role in marrubiin biosynthesis in the medicinal plant Marrubium vulgare
    journal, March 2019

    • Karunanithi, Prema S.; Dhanota, Puja; Addison, J. Bennett
    • BMC Plant Biology, Vol. 19, Issue 1
    • DOI: 10.1186/s12870-019-1702-5