Geometry-Dependent Nonequilibrium Steady-State Diffusion and Adsorption of Lipid Vesicles in Micropillar Arrays
Abstract
Micro-and nanofabricated sample environments are useful tools for characterizing diffusion in confined aqueous environments. The steady-state diffusion and adsorption of unilamellar lipid vesicles in arrays of hydrophilic micropillars is investigated. Gradients in the coverage of fluorescently labeled, pillar-supported lipid films, formed from vesicle fusion, are determined from 3D z-stack images using confocal microscopy. The gradients are the result of preferential adsorption of vesicles near the tops of the pillars, which progressively deplete them from solution as they diffuse toward the base of the array. However, the increased propensity for vesicle adsorption near the pillar tops compared to the confined spaces between pillars results in the formation of confluent supported lipid bilayers at the pillar tops that resist the adsorption of additional vesicles while leaving the pillar surfaces below available for binding. This results in a reduction in the numbers of depleted vesicles compared to what one would anticipate based on diffusive fluxes. The resulting inhomogeneous spatial profiles of lipid structures on the pillars are the result of the system being maintained in a dissipative, nonequilibrium steady state during incubation of the pillar arrays in the vesicle solution, which is ultimately quenched by rinsing away the unbound, freely diffusing vesicles.
- Authors:
-
[1];
[1];
[1];
[1];
[1]
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Univ. of Tennessee, Knoxville, TN (United States)
- Eastern Tennessee State Univ., Johnson City, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1546529
- Alternate Identifier(s):
- OSTI ID: 1501712
- Grant/Contract Number:
- AC05-00OR22725; DE‐AC05‐00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Advanced Materials Interfaces
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 9; Journal ID: ISSN 2196-7350
- Publisher:
- Wiley-VCH
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Liu, Fangjie, Abel, Steven M., Collins, Liam F., Srijanto, Bernadeta R., Standaert, Robert, Katsaras, John, and Collier, Pat. Geometry-Dependent Nonequilibrium Steady-State Diffusion and Adsorption of Lipid Vesicles in Micropillar Arrays. United States: N. p., 2019.
Web. doi:10.1002/admi.201900054.
Liu, Fangjie, Abel, Steven M., Collins, Liam F., Srijanto, Bernadeta R., Standaert, Robert, Katsaras, John, & Collier, Pat. Geometry-Dependent Nonequilibrium Steady-State Diffusion and Adsorption of Lipid Vesicles in Micropillar Arrays. United States. https://doi.org/10.1002/admi.201900054
Liu, Fangjie, Abel, Steven M., Collins, Liam F., Srijanto, Bernadeta R., Standaert, Robert, Katsaras, John, and Collier, Pat. Mon .
"Geometry-Dependent Nonequilibrium Steady-State Diffusion and Adsorption of Lipid Vesicles in Micropillar Arrays". United States. https://doi.org/10.1002/admi.201900054. https://www.osti.gov/servlets/purl/1546529.
@article{osti_1546529,
title = {Geometry-Dependent Nonequilibrium Steady-State Diffusion and Adsorption of Lipid Vesicles in Micropillar Arrays},
author = {Liu, Fangjie and Abel, Steven M. and Collins, Liam F. and Srijanto, Bernadeta R. and Standaert, Robert and Katsaras, John and Collier, Pat},
abstractNote = {Micro-and nanofabricated sample environments are useful tools for characterizing diffusion in confined aqueous environments. The steady-state diffusion and adsorption of unilamellar lipid vesicles in arrays of hydrophilic micropillars is investigated. Gradients in the coverage of fluorescently labeled, pillar-supported lipid films, formed from vesicle fusion, are determined from 3D z-stack images using confocal microscopy. The gradients are the result of preferential adsorption of vesicles near the tops of the pillars, which progressively deplete them from solution as they diffuse toward the base of the array. However, the increased propensity for vesicle adsorption near the pillar tops compared to the confined spaces between pillars results in the formation of confluent supported lipid bilayers at the pillar tops that resist the adsorption of additional vesicles while leaving the pillar surfaces below available for binding. This results in a reduction in the numbers of depleted vesicles compared to what one would anticipate based on diffusive fluxes. The resulting inhomogeneous spatial profiles of lipid structures on the pillars are the result of the system being maintained in a dissipative, nonequilibrium steady state during incubation of the pillar arrays in the vesicle solution, which is ultimately quenched by rinsing away the unbound, freely diffusing vesicles.},
doi = {10.1002/admi.201900054},
journal = {Advanced Materials Interfaces},
number = 9,
volume = 6,
place = {United States},
year = {Mon Mar 18 00:00:00 EDT 2019},
month = {Mon Mar 18 00:00:00 EDT 2019}
}
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