Metal Reduction and Protein Secretion Genes Required for Iodate Reduction by Shewanella oneidensis

Toporek, Yael J.; Mok, Jung Kee; Shin, Hyun Dong; Lee, Brady D.; Lee, M. Hope; DiChristina, Thomas J.; Müller, Volker

doi:10.1128/AEM.02115-18

Title: Metal Reduction and Protein Secretion Genes Required for Iodate Reduction by Shewanella oneidensis

Journal Article · 23 January 2019 · Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/AEM.02115-18 · OSTI ID:1501527

Toporek, Yael J. ^[1]; Mok, Jung Kee ^[1]; Shin, Hyun Dong ^[1]; Lee, Brady D. ^[2]; Lee, M. Hope ^[3];

^[1]; Müller, Volker ^[4]

Georgia Inst. of Technology, Atlanta, GA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Savannah River National Lab., Aiken, SC (United States)
Goethe Univ., Frankfurt am Main (Germany)

The metal-reducing gammaproteobacteriumShewanella oneidensisreduces iodate (IO₃^-) as an anaerobic terminal electron acceptor. Microbial IO₃^-electron transport pathways are postulated to terminate with nitrate (NO₃^-) reductase, which reduces IO₃^-as an alternative electron acceptor. Recent studies withS. oneidensis, however, have demonstrated that NO₃^-reductase is not involved in IO₃^-reduction. The main objective of the present study was to determine the metal reduction and protein secretion genes required for IO₃^-reduction byShewanella oneidensiswith lactate, formate, or H₂as the electron donor. With all electron donors, the type I and type V protein secretion mutants retained wild-type IO₃^-reduction activity, while the type II protein secretion mutant lacking the outer membrane secretin GspD was impaired in IO₃^-reduction. Deletion mutants lacking the cyclic AMP receptor protein (CRP), cytochrome maturation permease CcmB, and inner membrane-tetheredc-type cytochrome CymA were impaired in IO₃^-reduction with all electron donors, while deletion mutants lackingc-type cytochrome MtrA and outer membrane β-barrel protein MtrB of the outer membrane MtrAB module were impaired in IO₃^-reduction with only lactate as an electron donor. With all electron donors, mutants lacking thec-type cytochromes OmcA and MtrC of the metal-reducing extracellular electron conduit MtrCAB retained wild-type IO₃^-reduction activity. These findings indicate that IO₃^-reduction byS. oneidensisinvolves electron donor-dependent metal reduction and protein secretion pathway components, including the outer membrane MtrAB module and type II protein secretion of an unidentified IO₃^-reductase to theS. oneidensisouter membrane.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1501527

Report Number(s):: PNNL-SA-137826

Journal Information:: Applied and Environmental Microbiology, Vol. 85, Issue 3; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 16 works

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Shewanella oneidensis
iodate
iodine
metals
reduction

Title: Metal Reduction and Protein Secretion Genes Required for Iodate Reduction by Shewanella oneidensis

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