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Title: Genetic loci simultaneously controlling lignin monomers and biomass digestibility of rice straw

Lignin content and composition are crucial factors affecting biomass digestibility. Exploring the genetic loci simultaneously affecting lignin-relevant traits and biomass digestibility is a precondition for lignin genetic manipulation towards energy crop breeding. In this study, a high-throughput platform was employed to assay the lignin content, lignin composition and biomass enzymatic digestibility of a rice recombinant inbred line population. Correlation analysis indicated that the absolute content of lignin monomers rather than lignin content had negative effects on biomass saccharification, whereas the relative content of p-hydroxyphenyl unit and the molar ratio of p-hydroxyphenyl unit to guaiacyl unit exhibited positive roles. Eight QTL clusters were identified and four of them affecting both lignin composition and biomass digestibility. Here, the additive effects of clustered QTL revealed consistent relationships between lignin-relevant traits and biomass digestibility. Pyramiding rice lines containing the above four positive alleles for increasing biomass digestibility were selected and showed comparable lignin content, decreased syringyl or guaiacyl unit and increased molar percentage of p-hydroxyphenyl unit, the molar ratio of p-hydroxyphenyl unit to guaiacyl unit and sugar releases. More importantly, the lodging resistance and eating/cooking quality of pyramiding lines were not sacrificed, indicating the QTL information could be applied to select desirable energy ricemore » lines.« less
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [1] ;  [1] ; ORCiD logo [1]
  1. Huazhong Agricultural Univ., Wuhan (China)
  2. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Grant/Contract Number:
FC02-07ER64494
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1500120

Hu, Zhen, Zhang, Guifen, Muhammad, Ali, Samad, Rana Abdul, Wang, Youmei, Walton, Jonathan D., He, Yuqing, Peng, Liangcai, and Wang, Lingqiang. Genetic loci simultaneously controlling lignin monomers and biomass digestibility of rice straw. United States: N. p., Web. doi:10.1038/s41598-018-21741-y.
Hu, Zhen, Zhang, Guifen, Muhammad, Ali, Samad, Rana Abdul, Wang, Youmei, Walton, Jonathan D., He, Yuqing, Peng, Liangcai, & Wang, Lingqiang. Genetic loci simultaneously controlling lignin monomers and biomass digestibility of rice straw. United States. doi:10.1038/s41598-018-21741-y.
Hu, Zhen, Zhang, Guifen, Muhammad, Ali, Samad, Rana Abdul, Wang, Youmei, Walton, Jonathan D., He, Yuqing, Peng, Liangcai, and Wang, Lingqiang. 2018. "Genetic loci simultaneously controlling lignin monomers and biomass digestibility of rice straw". United States. doi:10.1038/s41598-018-21741-y. https://www.osti.gov/servlets/purl/1500120.
@article{osti_1500120,
title = {Genetic loci simultaneously controlling lignin monomers and biomass digestibility of rice straw},
author = {Hu, Zhen and Zhang, Guifen and Muhammad, Ali and Samad, Rana Abdul and Wang, Youmei and Walton, Jonathan D. and He, Yuqing and Peng, Liangcai and Wang, Lingqiang},
abstractNote = {Lignin content and composition are crucial factors affecting biomass digestibility. Exploring the genetic loci simultaneously affecting lignin-relevant traits and biomass digestibility is a precondition for lignin genetic manipulation towards energy crop breeding. In this study, a high-throughput platform was employed to assay the lignin content, lignin composition and biomass enzymatic digestibility of a rice recombinant inbred line population. Correlation analysis indicated that the absolute content of lignin monomers rather than lignin content had negative effects on biomass saccharification, whereas the relative content of p-hydroxyphenyl unit and the molar ratio of p-hydroxyphenyl unit to guaiacyl unit exhibited positive roles. Eight QTL clusters were identified and four of them affecting both lignin composition and biomass digestibility. Here, the additive effects of clustered QTL revealed consistent relationships between lignin-relevant traits and biomass digestibility. Pyramiding rice lines containing the above four positive alleles for increasing biomass digestibility were selected and showed comparable lignin content, decreased syringyl or guaiacyl unit and increased molar percentage of p-hydroxyphenyl unit, the molar ratio of p-hydroxyphenyl unit to guaiacyl unit and sugar releases. More importantly, the lodging resistance and eating/cooking quality of pyramiding lines were not sacrificed, indicating the QTL information could be applied to select desirable energy rice lines.},
doi = {10.1038/s41598-018-21741-y},
journal = {Scientific Reports},
number = 1,
volume = 8,
place = {United States},
year = {2018},
month = {2}
}

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