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Title: The Role of Chromatin Density in Cell Population Heterogeneity during Stem Cell Differentiation

Journal Article · · Scientific Reports
 [1];  [1];  [2];  [1];  [1]
  1. Univ. of California, Santa Barbara, CA (United States)
  2. Univ. of California, Santa Barbara, CA (United States); Uppsala Univ. (Sweden)

We incorporate three-dimensional (3D) conformation of chromosome (Hi-C) and single-cell RNA sequencing data together with discrete stochastic simulation, to explore the role of chromatin reorganization in determining gene expression heterogeneity during development. While previous research has emphasized the importance of chromatin architecture on activation and suppression of certain regulatory genes and gene networks, our study demonstrates how chromatin remodeling can dictate gene expression distribution by folding into distinct topological domains. We hypothesize that the local DNA density during differentiation accentuate transcriptional bursting due to the crowding effect of chromatin. This phenomenon yields a heterogeneous cell population, thereby increasing the potential of differentiation of the stem cells.

Research Organization:
Univ. of California, Santa Barbara, CA (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
SC0008975
OSTI ID:
1500036
Journal Information:
Scientific Reports, Vol. 7, Issue 1; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (5)

Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis journal August 2019
Chromatin Compaction, Auxeticity, and the Epigenetic Landscape of Stem Cells journal October 2019
You Do Not Mess with the Glia journal July 2018
Chromatin compaction states, nuclear shape fluctuations and auxeticity: A biophysical interpretation of the epigenetic landscape of stem cells posted_content September 2018
Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis text January 2019

Figures / Tables (4)