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Title: Transcriptomic analysis of transgressive segregants revealed the central role of photosynthetic capacity and efficiency in biomass accumulation in sugarcane

Abstract

Sugarcane is among the most efficient crops in converting solar energy into chemical energy. However, due to its complex genome structure and inheritance, the genetic and molecular basis of biomass yield in sugarcane is still largely unknown. We created an F2 segregating population by crossing S. officinarum and S. spontaneum and evaluated the biomass yield of the F2 individuals. The F2 individuals exhibited clear transgressive segregation in biomass yield. We sequenced transcriptomes of source and sink tissues from 12 selected extreme segregants to explore the molecular basis of high biomass yield for future breeding of high-yielding energy canes. Among the 103,664 assembled unigenes, 10,115 and 728 showed significant differential expression patterns between the two extreme segregating groups in the top visible dewlap leaf and the 9th culm internode, respectively. The most enriched functional categories were photosynthesis and fermentation in the high-biomass and the low-biomass groups, respectively. Our results revealed that high-biomass yield was mainly determined by assimilation of carbon in source tissues. The high-level expression of fermentative genes in the low-biomass group was likely induced by their low-energy status. Group-specific expression alleles which can be applied in the development of new high-yielding energy cane varieties via molecular breeding were identified.

Authors:
ORCiD logo [1];  [2];  [3];  [4];  [2];  [5]; ORCiD logo [6]
+ Show Author Affiliations
  1. Texas A & M Univ., College Station, TX (United States)
  2. Hawaii Agriculture Research Center, Kunia, HI (United States)
  3. Univ. of Illinois, Urbana-Champaign, IL (United States)
  4. Texas A & M Univ., Weslaco, TX (United States) AgriLife Research Center
  5. Univ. of Illinois, Urbana-Champaign, IL (United States); Fujian Agriculture and Forestry Univ., Fuzhou (China)
  6. Texas A & M Univ., College Station, TX (United States); Fujian Agriculture and Forestry Univ., Fuzhou (China)
Publication Date:
Research Org.:
Univ. of Illinois, Urbana-Champaign, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1500033
Grant/Contract Number:  
SC0010686
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Singh, Ratnesh, Jones, Tyler, Wai, Ching Man, Jifon, John, Nagai, Chifumi, Ming, Ray, and Yu, Qingyi. Transcriptomic analysis of transgressive segregants revealed the central role of photosynthetic capacity and efficiency in biomass accumulation in sugarcane. United States: N. p., 2018. Web. doi:10.1038/s41598-018-22798-5.
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Singh, Ratnesh, Jones, Tyler, Wai, Ching Man, Jifon, John, Nagai, Chifumi, Ming, Ray, & Yu, Qingyi. Transcriptomic analysis of transgressive segregants revealed the central role of photosynthetic capacity and efficiency in biomass accumulation in sugarcane. United States. doi:10.1038/s41598-018-22798-5.
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Singh, Ratnesh, Jones, Tyler, Wai, Ching Man, Jifon, John, Nagai, Chifumi, Ming, Ray, and Yu, Qingyi. Tue . "Transcriptomic analysis of transgressive segregants revealed the central role of photosynthetic capacity and efficiency in biomass accumulation in sugarcane". United States. doi:10.1038/s41598-018-22798-5. https://www.osti.gov/servlets/purl/1500033.
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@article{osti_1500033,
title = {Transcriptomic analysis of transgressive segregants revealed the central role of photosynthetic capacity and efficiency in biomass accumulation in sugarcane},
author = {Singh, Ratnesh and Jones, Tyler and Wai, Ching Man and Jifon, John and Nagai, Chifumi and Ming, Ray and Yu, Qingyi},
abstractNote = {Sugarcane is among the most efficient crops in converting solar energy into chemical energy. However, due to its complex genome structure and inheritance, the genetic and molecular basis of biomass yield in sugarcane is still largely unknown. We created an F2 segregating population by crossing S. officinarum and S. spontaneum and evaluated the biomass yield of the F2 individuals. The F2 individuals exhibited clear transgressive segregation in biomass yield. We sequenced transcriptomes of source and sink tissues from 12 selected extreme segregants to explore the molecular basis of high biomass yield for future breeding of high-yielding energy canes. Among the 103,664 assembled unigenes, 10,115 and 728 showed significant differential expression patterns between the two extreme segregating groups in the top visible dewlap leaf and the 9th culm internode, respectively. The most enriched functional categories were photosynthesis and fermentation in the high-biomass and the low-biomass groups, respectively. Our results revealed that high-biomass yield was mainly determined by assimilation of carbon in source tissues. The high-level expression of fermentative genes in the low-biomass group was likely induced by their low-energy status. Group-specific expression alleles which can be applied in the development of new high-yielding energy cane varieties via molecular breeding were identified.},
doi = {10.1038/s41598-018-22798-5},
journal = {Scientific Reports},
number = 1,
volume = 8,
place = {United States},
year = {2018},
month = {3}
}
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Journal Article:
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DOI:  10.1038/s41598-018-22798-5
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Figures / Tables:

Figure 1 Figure 1: Distribution of the stalk volumes of the S. officinarum × S. spontaneum F2 clones.
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    Works referencing / citing this record:

    Suberin and hemicellulose in sugarcane cell wall architecture and crop digestibility: A biotechnological perspective
    journal, February 2019

    • Figueiredo, Raquel; Araújo, Pedro; Llerena, Juan Pablo P.
    • Food and Energy Security, Vol. 8, Issue 3
    • DOI: 10.1002/fes3.163
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      Figures / Tables found in this record:

      Figure 1 (p. 2)figure
      Table 1 (p. 3)table
      Table 2 (p. 4)table
      Figure 2 (p. 5)figure
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      Table 3 (p. 6)table
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