Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway

Journal Article · 21 May 2018 · Nature Communications

DOI: https://doi.org/10.1038/s41467-018-04417-z · OSTI ID:1500021

Yu, Hong ^[1]; Li, Xiaoqian ^[2]; Duchoud, Fabienne ^[2]; Chuang, Derrick S. ^[2]; Liao, James C. ^[3]

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1. Univ. of California, Los Angeles, CA (United States); University of California, Los Angeles
2. Univ. of California, Los Angeles, CA (United States)
3. Academia Sinica, Taipei (Taiwan)

The Calvin–Benson–Bassham (CBB) cycle is presumably evolved for optimal synthesis of C3 sugars, but not for the production of C2 metabolite acetyl-CoA. The carbon loss in producing acetyl-CoA from decarboxylation of C3 sugar limits the maximum carbon yield of photosynthesis. Here we design a synthetic malyl-CoA-glycerate (MCG) pathway to augment the CBB cycle for efficient acetyl-CoA synthesis. This pathway converts a C3 metabolite to two acetyl-CoA by fixation of one additional CO2 equivalent, or assimilates glyoxylate, a photorespiration intermediate, to produce acetyl-CoA without net carbon loss. We first functionally demonstrate the design of the MCG pathway in vitro and in Escherichia coli. We then implement the pathway in a photosynthetic organism Synechococcus elongates PCC7942, and show that it increases the intracellular acetyl-CoA pool and enhances bicarbonate assimilation by roughly 2-fold. This work provides a strategy to improve carbon fixation efficiency in photosynthetic organisms.

• Research Organization: Univ. of California, Los Angeles, CA (United States)
• Sponsoring Organization: USDOE Advanced Research Projects Agency - Energy (ARPA-E); USDOE
• Grant/Contract Number: AR0000201; FC03-02ER63421
• OSTI ID: 1500021
• Journal Information: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 9; ISSN 2041-1723
• Publisher: Nature Publishing GroupCopyright Statement
• Country of Publication: United States
• Language: English