skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Highly selective and sensitive macrocycle-based dinuclear foldamer for fluorometric and colorimetric sensing of citrate in water

Abstract

The selective detection of citrate anions is essential for various biological functions in living systems. A quantitative assessment of citrate is required for the diagnosis of various diseases in the human body; however, it is extremely challenging to develop efficient fluorescence and color-detecting molecular probes for sensing citrate in water. Herein, we report a macrocycle-based dinuclear foldamer (1) assembled with eosin Y (EY) that has been studied for anion binding by fluorescence and colorimetric techniques in water at neutral pH. Results from the fluorescence titrations reveal that the 1·EY ensemble strongly binds citrate anions, showing remarkable selectivity over a wide range of inorganic and carboxylate anions. The addition of citrate anions to the 1·EY adduct led to a large fluorescence enhancement, displaying a detectable color change under both visible and UV light in water up to 2 μmol. The biocompatibility of 1·EY as an intracellular carrier in a biological system was evaluated on primary human foreskin fibroblast (HF) cells, showing an excellent cell viability. The strong binding properties of the ensemble allow it to be used as a highly sensitive, detective probe for biologically relevant citrate anions in various applications.

Authors:
 [1];  [2];  [1];  [3];  [4]; ORCiD logo [3]; ORCiD logo [2];  [1]
  1. Jackson State Univ, Jackson, MS (United States)
  2. Univ. of Mississippi Medical Center, Jackson, MS (United States)
  3. Univ. of California, Riverside, CA (United States)
  4. Univ. of Oklahoma, Norman, OK (United States)
Publication Date:
Research Org.:
Univ. of California, Riverside, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1500015
Grant/Contract Number:  
SC0016269
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Rhaman, Md Mhahabubur, Hasan, Mohammad H., Alamgir, Azmain, Xu, Lihua, Powell, Douglas R., Wong, Bryan M., Tandon, Ritesh, and Hossain, Md. Alamgir. Highly selective and sensitive macrocycle-based dinuclear foldamer for fluorometric and colorimetric sensing of citrate in water. United States: N. p., 2018. Web. doi:10.1038/s41598-017-18322-w.
Rhaman, Md Mhahabubur, Hasan, Mohammad H., Alamgir, Azmain, Xu, Lihua, Powell, Douglas R., Wong, Bryan M., Tandon, Ritesh, & Hossain, Md. Alamgir. Highly selective and sensitive macrocycle-based dinuclear foldamer for fluorometric and colorimetric sensing of citrate in water. United States. doi:10.1038/s41598-017-18322-w.
Rhaman, Md Mhahabubur, Hasan, Mohammad H., Alamgir, Azmain, Xu, Lihua, Powell, Douglas R., Wong, Bryan M., Tandon, Ritesh, and Hossain, Md. Alamgir. Wed . "Highly selective and sensitive macrocycle-based dinuclear foldamer for fluorometric and colorimetric sensing of citrate in water". United States. doi:10.1038/s41598-017-18322-w. https://www.osti.gov/servlets/purl/1500015.
@article{osti_1500015,
title = {Highly selective and sensitive macrocycle-based dinuclear foldamer for fluorometric and colorimetric sensing of citrate in water},
author = {Rhaman, Md Mhahabubur and Hasan, Mohammad H. and Alamgir, Azmain and Xu, Lihua and Powell, Douglas R. and Wong, Bryan M. and Tandon, Ritesh and Hossain, Md. Alamgir},
abstractNote = {The selective detection of citrate anions is essential for various biological functions in living systems. A quantitative assessment of citrate is required for the diagnosis of various diseases in the human body; however, it is extremely challenging to develop efficient fluorescence and color-detecting molecular probes for sensing citrate in water. Herein, we report a macrocycle-based dinuclear foldamer (1) assembled with eosin Y (EY) that has been studied for anion binding by fluorescence and colorimetric techniques in water at neutral pH. Results from the fluorescence titrations reveal that the 1·EY ensemble strongly binds citrate anions, showing remarkable selectivity over a wide range of inorganic and carboxylate anions. The addition of citrate anions to the 1·EY adduct led to a large fluorescence enhancement, displaying a detectable color change under both visible and UV light in water up to 2 μmol. The biocompatibility of 1·EY as an intracellular carrier in a biological system was evaluated on primary human foreskin fibroblast (HF) cells, showing an excellent cell viability. The strong binding properties of the ensemble allow it to be used as a highly sensitive, detective probe for biologically relevant citrate anions in various applications.},
doi = {10.1038/s41598-017-18322-w},
journal = {Scientific Reports},
number = 1,
volume = 8,
place = {United States},
year = {2018},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share:

Works referenced in this record:

Highly Selective Oligonucleotide-Based Sensor for Mercury(II) in Aqueous Solutions
journal, August 2004

  • Ono, Akira; Togashi, Humika
  • Angewandte Chemie International Edition, Vol. 43, Issue 33, p. 4300-4302
  • DOI: 10.1002/anie.200454172