Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration

doi:10.1126/sciadv.1601453

Title: Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration

We developed membrane voltage nanosensors that are based on inorganic semiconductor nanoparticles. We provide here a feasibility study for their utilization. We use a rationally designed peptide to functionalize the nanosensors, imparting them with the ability to self-insert into a lipid membrane with a desired orientation. Once inserted, these nanosensors could sense membrane potential via the quantum confined Stark effect, with a single-particle sensitivity. With further improvements, these nanosensors could potentially be used for simultaneous recording of action potentials from multiple neurons in a large field of view over a long duration and for recording electrical signals on the nanoscale, such as across one synapse.

Authors:

Park, Kyoungwon ^[1] ; ; ; ; Dai, Xinghong ^[3] ; ; Kim, Wookyeom ^[5] ; ; Zou, Peng ^[7] ; Levine, Alex J. ^[8] ; Li, Jack ^[1] ;

Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry
Academy of Sciences of the Czech Republic (ASCR), Prague (Czech Republic). Inst. of Organic Chemistry and Biochemistry
Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics
Univ. of California, Los Angeles, CA (United States). Dept. of Chemical and Biomolecular Engineering
Univ. of California, Los Angeles, CA (United States). Dept. of Ecology and Evolutionary Biology
Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics. California NanoSystems Inst.
Harvard Univ., Cambridge, MA (United States). Dept. of Chemistry and Chemical Biology
Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry. Dept. of Physics
Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry. California NanoSystems Inst. Dept. of Physiology

Publication Date:: 2018-01-12

Grant/Contract Number:: FC02-02ER63421; AC02-05CH11231; 1S10RR23057; GM071940; DMR-1309188; DMR-1548924; D14PC00141; 2010382; RGP0061/2015

Type:: Accepted Manuscript

Journal Name:: Science Advances

Additional Journal Information:: Journal Volume: 4; Journal Issue: 1; Journal ID: ISSN 2375-2548

Publisher:: AAAS

Research Org:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Org:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Inst. of Health (NIH) (United States); National Science Foundation (NSF); Defense Advanced Research Projects Agency (DARPA) (United States); United States–Israel Binational Science Foundation; Human Frontier Science Program

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 36 MATERIALS SCIENCE

OSTI Identifier:: 1499965


                    Park, Kyoungwon, Kuo, Yung, Shvadchak, Volodymyr, Ingargiola, Antonino, Dai, Xinghong, Hsiung, Lawrence, Kim, Wookyeom, Zhou, Hong, Zou, Peng, Levine, Alex J., Li, Jack, and Weiss, Shimon. Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration.  United States: N. p.,  
        Web.  doi:10.1126/sciadv.1601453.

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                    Park, Kyoungwon, Kuo, Yung, Shvadchak, Volodymyr, Ingargiola, Antonino, Dai, Xinghong, Hsiung, Lawrence, Kim, Wookyeom, Zhou, Hong, Zou, Peng, Levine, Alex J., Li, Jack, & Weiss, Shimon. Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration.  United States.  doi:10.1126/sciadv.1601453.

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                    Park, Kyoungwon, Kuo, Yung, Shvadchak, Volodymyr, Ingargiola, Antonino, Dai, Xinghong, Hsiung, Lawrence, Kim, Wookyeom, Zhou, Hong, Zou, Peng, Levine, Alex J., Li, Jack, and Weiss, Shimon. 2018.  
        "Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration".  United States.  
        doi:10.1126/sciadv.1601453.  https://www.osti.gov/servlets/purl/1499965.

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@article{osti_1499965,

  title        = {Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration},

  author       = {Park, Kyoungwon and Kuo, Yung and Shvadchak, Volodymyr and Ingargiola, Antonino and Dai, Xinghong and Hsiung, Lawrence and Kim, Wookyeom and Zhou, Hong and Zou, Peng and Levine, Alex J. and Li, Jack and Weiss, Shimon},

  abstractNote = {We developed membrane voltage nanosensors that are based on inorganic semiconductor nanoparticles. We provide here a feasibility study for their utilization. We use a rationally designed peptide to functionalize the nanosensors, imparting them with the ability to self-insert into a lipid membrane with a desired orientation. Once inserted, these nanosensors could sense membrane potential via the quantum confined Stark effect, with a single-particle sensitivity. With further improvements, these nanosensors could potentially be used for simultaneous recording of action potentials from multiple neurons in a large field of view over a long duration and for recording electrical signals on the nanoscale, such as across one synapse.},

  doi          = {10.1126/sciadv.1601453},

  journal      = {Science Advances},

  number       = 1,

  volume       = 4,

  place        = {United States},

  year         = {2018},

  month        = {1}

}

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Free Publicly Accessible Full Text
Accepted Manuscript (DOE)
Publisher's Version of Record
10.1126/sciadv.1601453
https://doi.org/10.1126/sciadv.1601453

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Synthesis of Soluble and Processable Rod-, Arrow-, Teardrop-, and Tetrapod-Shaped CdSe Nanocrystals
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Nature, Vol. 404, Issue 6773, p. 59-61
DOI: 10.1038/35003535

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Title: Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration

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