Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration
Abstract
We developed membrane voltage nanosensors that are based on inorganic semiconductor nanoparticles. We provide here a feasibility study for their utilization. We use a rationally designed peptide to functionalize the nanosensors, imparting them with the ability to self-insert into a lipid membrane with a desired orientation. Once inserted, these nanosensors could sense membrane potential via the quantum confined Stark effect, with a single-particle sensitivity. With further improvements, these nanosensors could potentially be used for simultaneous recording of action potentials from multiple neurons in a large field of view over a long duration and for recording electrical signals on the nanoscale, such as across one synapse.
- Authors:
-
- Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry
- Academy of Sciences of the Czech Republic (ASCR), Prague (Czech Republic). Inst. of Organic Chemistry and Biochemistry
- Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics
- Univ. of California, Los Angeles, CA (United States). Dept. of Chemical and Biomolecular Engineering
- Univ. of California, Los Angeles, CA (United States). Dept. of Ecology and Evolutionary Biology
- Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics. California NanoSystems Inst.
- Harvard Univ., Cambridge, MA (United States). Dept. of Chemistry and Chemical Biology
- Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry. Dept. of Physics
- Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry. California NanoSystems Inst. Dept. of Physiology
- Publication Date:
- Research Org.:
- Univ. of California, Los Angeles, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Inst. of Health (NIH) (United States); National Science Foundation (NSF); Defense Advanced Research Projects Agency (DARPA) (United States); United States–Israel Binational Science Foundation; Human Frontier Science Program
- OSTI Identifier:
- 1499965
- Grant/Contract Number:
- FC02-02ER63421; AC02-05CH11231; 1S10RR23057; GM071940; DMR-1309188; DMR-1548924; D14PC00141; 2010382; RGP0061/2015
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Science Advances
- Additional Journal Information:
- Journal Volume: 4; Journal Issue: 1; Journal ID: ISSN 2375-2548
- Publisher:
- AAAS
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 36 MATERIALS SCIENCE
Citation Formats
Park, Kyoungwon, Kuo, Yung, Shvadchak, Volodymyr, Ingargiola, Antonino, Dai, Xinghong, Hsiung, Lawrence, Kim, Wookyeom, Zhou, Hong, Zou, Peng, Levine, Alex J., Li, Jack, and Weiss, Shimon. Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration. United States: N. p., 2018.
Web. doi:10.1126/sciadv.1601453.
Park, Kyoungwon, Kuo, Yung, Shvadchak, Volodymyr, Ingargiola, Antonino, Dai, Xinghong, Hsiung, Lawrence, Kim, Wookyeom, Zhou, Hong, Zou, Peng, Levine, Alex J., Li, Jack, & Weiss, Shimon. Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration. United States. https://doi.org/10.1126/sciadv.1601453
Park, Kyoungwon, Kuo, Yung, Shvadchak, Volodymyr, Ingargiola, Antonino, Dai, Xinghong, Hsiung, Lawrence, Kim, Wookyeom, Zhou, Hong, Zou, Peng, Levine, Alex J., Li, Jack, and Weiss, Shimon. Fri .
"Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration". United States. https://doi.org/10.1126/sciadv.1601453. https://www.osti.gov/servlets/purl/1499965.
@article{osti_1499965,
title = {Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration},
author = {Park, Kyoungwon and Kuo, Yung and Shvadchak, Volodymyr and Ingargiola, Antonino and Dai, Xinghong and Hsiung, Lawrence and Kim, Wookyeom and Zhou, Hong and Zou, Peng and Levine, Alex J. and Li, Jack and Weiss, Shimon},
abstractNote = {We developed membrane voltage nanosensors that are based on inorganic semiconductor nanoparticles. We provide here a feasibility study for their utilization. We use a rationally designed peptide to functionalize the nanosensors, imparting them with the ability to self-insert into a lipid membrane with a desired orientation. Once inserted, these nanosensors could sense membrane potential via the quantum confined Stark effect, with a single-particle sensitivity. With further improvements, these nanosensors could potentially be used for simultaneous recording of action potentials from multiple neurons in a large field of view over a long duration and for recording electrical signals on the nanoscale, such as across one synapse.},
doi = {10.1126/sciadv.1601453},
journal = {Science Advances},
number = 1,
volume = 4,
place = {United States},
year = {2018},
month = {1}
}
Web of Science
Figures / Tables:

Works referenced in this record:
Optically monitoring voltage in neurons by photo-induced electron transfer through molecular wires
journal, January 2012
- Miller, E. W.; Lin, J. Y.; Frady, E. P.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 6
Fusion of cationic liposomes with mammalian cells occurs after endocytosis
journal, May 1995
- Wrobel, Iwona; Collins, David
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1235, Issue 2
Breakthroughs in the Development of Semiconductor-Sensitized Solar Cells
journal, October 2010
- Mora-Seró, Iván; Bisquert, Juan
- The Journal of Physical Chemistry Letters, Vol. 1, Issue 20
Bilayer-Spanning DNA Nanopores with Voltage-Switching between Open and Closed State
journal, December 2014
- Seifert, Astrid; Göpfrich, Kerstin; Burns, Jonathan R.
- ACS Nano, Vol. 9, Issue 2
Synthesis and characterization of nearly monodisperse CdE (E = sulfur, selenium, tellurium) semiconductor nanocrystallites
journal, September 1993
- Murray, C. B.; Norris, D. J.; Bawendi, M. G.
- Journal of the American Chemical Society, Vol. 115, Issue 19, p. 8706-8715
Synthesis of Soluble and Processable Rod-, Arrow-, Teardrop-, and Tetrapod-Shaped CdSe Nanocrystals
December 2000
- Manna, Liberato; Scher, Erik C.; Alivisatos, A. Paul
- Journal of the American Chemical Society, Vol. 122, Issue 51, p. 12700-12706
Dual-view microscopy with a single camera: real-time imaging of molecular orientations and calcium
journal, October 1991
- Kinosita, K.
- The Journal of Cell Biology, Vol. 115, Issue 1
Allosteric regulation of DegS protease subunits through a shared energy landscape
journal, December 2012
- Mauldin, Randall V.; Sauer, Robert T.
- Nature Chemical Biology, Vol. 9, Issue 2
Advances in fluorescence imaging with quantum dot bio-probes
journal, March 2006
- Pinaud, Fabien; Michalet, Xavier; Bentolila, Laurent A.
- Biomaterials, Vol. 27, Issue 9
Seeded Growth of Highly Luminescent CdSe/CdS Nanoheterostructures with Rod and Tetrapod Morphologies
journal, October 2007
- Talapin, Dmitri V.; Nelson, James H.; Shevchenko, Elena V.
- Nano Letters, Vol. 7, Issue 10
Design Rules for Membrane-Embedded Voltage-Sensing Nanoparticles
journal, February 2017
- Park, Kyoungwon; Weiss, Shimon
- Biophysical Journal, Vol. 112, Issue 4
Near Unity Quantum Yield of Light-Driven Redox Mediator Reduction and Efficient H 2 Generation Using Colloidal Nanorod Heterostructures
journal, July 2012
- Zhu, Haiming; Song, Nianhui; Lv, Hongjin
- Journal of the American Chemical Society, Vol. 134, Issue 28
Interactive notebooks: Sharing the code
journal, November 2014
- Shen, Helen
- Nature, Vol. 515, Issue 7525
Nearly Monodisperse and Shape-Controlled CdSe Nanocrystals via Alternative Routes: Nucleation and Growth
journal, April 2002
- Peng, Z. Adam; Peng, Xiaogang
- Journal of the American Chemical Society, Vol. 124, Issue 13, p. 3343-3353
Azamacrocycle Activated Quantum Dot for Zinc Ion Detection
journal, November 2008
- Ruedas-Rama, Maria Jose; Hall, Elizabeth A. H.
- Analytical Chemistry, Vol. 80, Issue 21
Stochastic transport through carbon nanotubes in lipid bilayers and live cell membranes
journal, October 2014
- Geng, Jia; Kim, Kyunghoon; Zhang, Jianfei
- Nature, Vol. 514, Issue 7524
Fusogenic activity of cationic lipids and lipid shape distribution
journal, November 2009
- Lonez, Caroline; Lensink, Marc F.; Kleiren, Emilie
- Cellular and Molecular Life Sciences, Vol. 67, Issue 3
Single-exciton optical gain in semiconductor nanocrystals
journal, May 2007
- Klimov, Victor I.; Ivanov, Sergei A.; Nanda, Jagjit
- Nature, Vol. 447, Issue 7143
Wave Function Engineering in Elongated Semiconductor Nanocrystals with Heterogeneous Carrier Confinement
journal, October 2005
- Müller, J.; Lupton, J. M.; Lagoudakis, P. G.
- Nano Letters, Vol. 5, Issue 10
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics
journal, January 2005
- Michalet, X.
- Science, Vol. 307, Issue 5709
Incorporation of quantum dots into the lipid bilayer of giant unilamellar vesicles and its stability
journal, September 2012
- Wi, Haeng Sub; Kim, Seong Jin; Lee, Kyuyong
- Colloids and Surfaces B: Biointerfaces, Vol. 97
Application of Thiolated Gold Nanoparticles for the Enhancement of Glucose Oxidase Activity
journal, March 2007
- Pandey, Pratibha; Singh, Surinder P.; Arya, Sunil K.
- Langmuir, Vol. 23, Issue 6
Nanoblade Delivery and Incorporation of Quantum Dot Conjugates into Tubulin Networks in Live Cells
journal, October 2012
- Xu, Jianmin; Teslaa, Tara; Wu, Ting-Hsiang
- Nano Letters, Vol. 12, Issue 11
Quantum dot bioconjugates for imaging, labelling and sensing
journal, June 2005
- Medintz, Igor L.; Uyeda, H. Tetsuo; Goldman, Ellen R.
- Nature Materials, Vol. 4, Issue 6, p. 435-446
Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization
journal, June 1979
- Axelrod, D.
- Biophysical Journal, Vol. 26, Issue 3
Synthetic Lipid Membrane Channels Formed by Designed DNA Nanostructures
journal, November 2012
- Langecker, M.; Arnaut, V.; Martin, T. G.
- Science, Vol. 338, Issue 6109
Single Quantum Dots as Local Temperature Markers
journal, October 2007
- Li, Sha; Zhang, Kai; Yang, Jui-Ming
- Nano Letters, Vol. 7, Issue 10
Solubilization of Quantum Dots with a Recombinant Peptide fromEscherichia coli
journal, May 2007
- Iyer, Gopal; Pinaud, Fabien; Tsay, James
- Small, Vol. 3, Issue 5
Auger Recombination Suppression in Nanocrystals with Asymmetric Electron-Hole Confinement
journal, January 2012
- Climente, Juan I.; Movilla, Jose L.; Planelles, Josep
- Small, Vol. 8, Issue 5
DNA-based programming of quantum dot valency, self-assembly and luminescence
journal, July 2011
- Tikhomirov, Grigory; Hoogland, Sjoerd; Lee, P. E.
- Nature Nanotechnology, Vol. 6, Issue 8
Highly Emissive Colloidal CdSe/CdS Heterostructures of Mixed Dimensionality
journal, December 2003
- Talapin, Dmitri V.; Koeppe, Robert; Götzinger, Stephan
- Nano Letters, Vol. 3, Issue 12
Full-colour quantum dot displays fabricated by transfer printing
journal, February 2011
- Kim, Tae-Ho; Cho, Kyung-Sang; Lee, Eun Kyung
- Nature Photonics, Vol. 5, Issue 3
Single Molecule Quantum-Confined Stark Effect Measurements of Semiconductor Nanoparticles at Room Temperature
journal, October 2012
- Park, KyoungWon; Deutsch, Zvicka; Li, J. Jack
- ACS Nano, Vol. 6, Issue 11
Electrical control of Förster energy transfer
journal, September 2006
- Becker, Klaus; Lupton, John M.; Müller, Josef
- Nature Materials, Vol. 5, Issue 10
Ez, a Depth-dependent Potential for Assessing the Energies of Insertion of Amino Acid Side-chains into Membranes: Derivation and Applications to Determining the Orientation of Transmembrane and Interfacial Helices
journal, February 2007
- Senes, Alessandro; Chadi, Deborah C.; Law, Peter B.
- Journal of Molecular Biology, Vol. 366, Issue 2
Enhancing the Photoluminescence of Peptide-Coated Nanocrystals with Shell Composition and UV Irradiation
journal, February 2005
- Tsay, James M.; Doose, Sören; Pinaud, Fabien
- The Journal of Physical Chemistry B, Vol. 109, Issue 5
Shape control of CdSe nanocrystals
journal, March 2000
- Peng, Xiaogang; Manna, Liberato; Yang, Weidong
- Nature, Vol. 404, Issue 6773, p. 59-61
Bioactivation and Cell Targeting of Semiconductor CdSe/ZnS Nanocrystals with Phytochelatin-Related Peptides
journal, May 2004
- Pinaud, Fabien; King, David; Moore, Hsiao-Ping
- Journal of the American Chemical Society, Vol. 126, Issue 19
Recent progress in the bioconjugation of quantum dots
journal, March 2014
- Blanco-Canosa, Juan B.; Wu, Miao; Susumu, Kimihiro
- Coordination Chemistry Reviews, Vol. 263-264
Monitoring surface charge migration in the spectral dynamics of single nanodot/nanorod heterostructures
journal, November 2005
- Müller, J.; Lupton, J. M.; Rogach, A. L.
- Physical Review B, Vol. 72, Issue 20
Self-Assembled Quantum Dot−Peptide Bioconjugates for Selective Intracellular Delivery
journal, July 2006
- Delehanty, James B.; Medintz, Igor L.; Pons, Thomas
- Bioconjugate Chemistry, Vol. 17, Issue 4
Quantum-dot/dopamine bioconjugates function as redox coupled assemblies for in vitro and intracellular pH sensing
journal, August 2010
- Medintz, Igor L.; Stewart, Michael H.; Trammell, Scott A.
- Nature Materials, Vol. 9, Issue 8
Colloidal Quantum Dots as Probes of Excitation Field Enhancement in Photonic Antennas
journal, July 2010
- Aouani, Heykel; Itzhakov, Stella; Gachet, David
- ACS Nano, Vol. 4, Issue 8
Radiative Recombination of Spatially Extended Excitons in (ZnSe/CdS)/CdS Heterostructured Nanorods
journal, January 2009
- Hewa-Kasakarage, Nishshanka N.; Kirsanova, Maria; Nemchinov, Alexander
- Journal of the American Chemical Society, Vol. 131, Issue 3
Screening Fluorescent Voltage Indicators with Spontaneously Spiking HEK Cells
journal, December 2013
- Park, Jeehae; Werley, Christopher A.; Venkatachalam, Veena
- PLoS ONE, Vol. 8, Issue 12
Fluorescence intermittency in single cadmium selenide nanocrystals
journal, October 1996
- Nirmal, M.; Dabbousi, B. O.; Bawendi, M. G.
- Nature, Vol. 383, Issue 6603
Particle Size, Surface Coating, and PEGylation Influence the Biodistribution of Quantum Dots in Living Mice
journal, January 2009
- Schipper, Meike L.; Iyer, Gopal; Koh, Ai Leen
- Small, Vol. 5, Issue 1
Multifunctional Lipid/Quantum Dot Hybrid Nanocontainers for Controlled Targeting of Live Cells
journal, August 2006
- Gopalakrishnan, Gopakumar; Danelon, Christophe; Izewska, Paulina
- Angewandte Chemie International Edition, Vol. 45, Issue 33
High Affinity scFv−Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells
journal, December 2008
- Iyer, Gopal; Michalet, Xavier; Chang, Yun-Pei
- Nano Letters, Vol. 8, Issue 12
Stable, Compact, Bright Biofunctional Quantum Dots with Improved Peptide Coating
journal, August 2012
- Xu, Jianmin; Ruchala, Piotr; Ebenstain, Yuval
- The Journal of Physical Chemistry B, Vol. 116, Issue 36
FRET between CdSe Quantum Dots in Lipid Vesicles and Water- and Lipid-soluble Dyes
journal, November 2004
- Kloepfer, Jeremiah A.; Cohen, Netta; Nadeau, Jay L.
- The Journal of Physical Chemistry B, Vol. 108, Issue 44
Lipid−Quantum Dot Bilayer Vesicles Enhance Tumor Cell Uptake and Retention in Vitro and in Vivo
journal, March 2008
- Al-Jamal, Wafa’ T.; Al-Jamal, Khuloud T.; Tian, Bowen
- ACS Nano, Vol. 2, Issue 3
Bright future of optical assays for ion channel drug discovery
journal, January 2008
- Molokanova, E.
- Drug Discovery Today, Vol. 13, Issue 1-2
Room-Temperature Exciton Storage in Elongated Semiconductor Nanocrystals
journal, January 2007
- Kraus, R. M.; Lagoudakis, P. G.; Rogach, A. L.
- Physical Review Letters, Vol. 98, Issue 1
Optical Strategies for Sensing Neuronal Voltage Using Quantum Dots and Other Semiconductor Nanocrystals
journal, April 2013
- Marshall, Jesse D.; Schnitzer, Mark J.
- ACS Nano, Vol. 7, Issue 5
Multifunctional Lipid/Quantum Dot Hybrid Nanocontainers for Controlled Targeting of Live Cells
journal, August 2006
- Gopalakrishnan, Gopakumar; Danelon, Christophe; Izewska, Paulina
- Angewandte Chemie, Vol. 118, Issue 33
Synthetic Lipid Membrane Channels formed by Designed DNA Nanostructures
journal, January 2013
- Langecker, Martin; Arnaut, Vera; Martin, Thomas G.
- Biophysical Journal, Vol. 104, Issue 2
Interactive notebooks: Sharing the code
journal, November 2014
- Shen, Helen
- Nature, Vol. 515, Issue 7525
Single molecule quantum-confined Stark effect measurements of semiconductor nanoparticles at room temperature
conference, February 2013
- Park, Kyoung Won; Deutsch, Zvicka; Li, J. Jack
- SPIE BiOS, SPIE Proceedings
Works referencing / citing this record:
Interfacing the Cell with “Biomimetic Membrane Proteins”
journal, December 2019
- Grupi, Asaf; Ashur, Idan; Degani‐Katzav, Nurit
- Small, Vol. 15, Issue 52
Strategies to Overcome Autofluorescence in Nanoprobe-Driven In Vivo Fluorescence Imaging
journal, June 2018
- del Rosal, Blanca; Benayas, Antonio
- Small Methods, Vol. 2, Issue 9
Sensing with photoluminescent semiconductor quantum dots
journal, January 2019
- Chern, Margaret; Kays, Joshua C.; Bhuckory, Shashi
- Methods and Applications in Fluorescence, Vol. 7, Issue 1
Figures / Tables found in this record: