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Title: Antioxidant metabolism variation associated with alkali-salt tolerance in thirty switchgrass (Panicum virgatum) lines

Abstract

Soil salinization is a major factor limiting crop growth and development in many areas. Switchgrass (Panicum virgatum L.) is an important warm-season grass species used for biofuel production. The objective of this study was to investigate antioxidant metabolism, proline,and protein variation associated with alkali-salt tolerance among 30 switchgrass lines and identify metabolic markers for evaluating alkali-salt tolerance of switchgrass lines. The grass lines were transplanted into plastic pots containing fine sand. When the plants reached E5 developmental stage, they were subjected to either alkali-salt stress treatment (150 mM Na + and pH of 9.5) or control (no alkali-salt stress) for 20 d. The 30 switchgrass lines differed in alkali-salt tolerance as determined by the level of leaf malondialdehyde (MDA), antioxidant enzyme activity [(superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX)], proline and protein. Alkali-salt stress increased MDA, proline, SOD, reduced CAT activity, but its effect on protein and APX varied depending on lines. Wide variations in the five parameters existed among the 30 lines. In general, the lines with higher CAT activity and lower proline content under alkali-salt stress had less MDA, exhibiting better alkali-salt tolerance. Among the five parameters, CAT can be considered as valuable metabolic markers for assessmentmore » of switchgrass tolerance to alkali-salt stress.« less

Authors:
 [1];  [2];  [3];  [4];  [3];  [3];  [3];  [5]; ORCiD logo [6]
  1. Northeast Agricultural Univ., Harbin, Heilongjiang Province (China). College of Animal Science and Technology; Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Crop and Soil Environmental Science
  2. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Crop and Soil Environmental Science; Chinese Academy of Tropical Agricultural Sciences (CATAS), Hainan Danzhou (China). Tropical Crops Genetic Resources Inst.
  3. Northeast Agricultural Univ., Harbin, Heilongjiang Province (China). College of Animal Science and Technology
  4. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Horticulture
  5. Univ. of Delaware, Newark, DE (United States). Dept. of Plant and Soil Sciences
  6. Virginia Polytechnic Inst. and State Univ. (Virginia Tech), Blacksburg, VA (United States). Dept. of Crop and Soil Environmental Science
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1499879
Grant/Contract Number:  
SC0008338
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 13; Journal Issue: 6; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Hu, Guofu, Liu, Yiming, Duo, Tianqi, Zhao, Bingyu, Cui, Guowen, Ji, Jing, Kuang, Xiao, Ervin, Erik H., and Zhang, Xunzhong. Antioxidant metabolism variation associated with alkali-salt tolerance in thirty switchgrass (Panicum virgatum) lines. United States: N. p., 2018. Web. doi:10.1371/journal.pone.0199681.
Hu, Guofu, Liu, Yiming, Duo, Tianqi, Zhao, Bingyu, Cui, Guowen, Ji, Jing, Kuang, Xiao, Ervin, Erik H., & Zhang, Xunzhong. Antioxidant metabolism variation associated with alkali-salt tolerance in thirty switchgrass (Panicum virgatum) lines. United States. doi:10.1371/journal.pone.0199681.
Hu, Guofu, Liu, Yiming, Duo, Tianqi, Zhao, Bingyu, Cui, Guowen, Ji, Jing, Kuang, Xiao, Ervin, Erik H., and Zhang, Xunzhong. Mon . "Antioxidant metabolism variation associated with alkali-salt tolerance in thirty switchgrass (Panicum virgatum) lines". United States. doi:10.1371/journal.pone.0199681. https://www.osti.gov/servlets/purl/1499879.
@article{osti_1499879,
title = {Antioxidant metabolism variation associated with alkali-salt tolerance in thirty switchgrass (Panicum virgatum) lines},
author = {Hu, Guofu and Liu, Yiming and Duo, Tianqi and Zhao, Bingyu and Cui, Guowen and Ji, Jing and Kuang, Xiao and Ervin, Erik H. and Zhang, Xunzhong},
abstractNote = {Soil salinization is a major factor limiting crop growth and development in many areas. Switchgrass (Panicum virgatum L.) is an important warm-season grass species used for biofuel production. The objective of this study was to investigate antioxidant metabolism, proline,and protein variation associated with alkali-salt tolerance among 30 switchgrass lines and identify metabolic markers for evaluating alkali-salt tolerance of switchgrass lines. The grass lines were transplanted into plastic pots containing fine sand. When the plants reached E5 developmental stage, they were subjected to either alkali-salt stress treatment (150 mM Na+ and pH of 9.5) or control (no alkali-salt stress) for 20 d. The 30 switchgrass lines differed in alkali-salt tolerance as determined by the level of leaf malondialdehyde (MDA), antioxidant enzyme activity [(superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX)], proline and protein. Alkali-salt stress increased MDA, proline, SOD, reduced CAT activity, but its effect on protein and APX varied depending on lines. Wide variations in the five parameters existed among the 30 lines. In general, the lines with higher CAT activity and lower proline content under alkali-salt stress had less MDA, exhibiting better alkali-salt tolerance. Among the five parameters, CAT can be considered as valuable metabolic markers for assessment of switchgrass tolerance to alkali-salt stress.},
doi = {10.1371/journal.pone.0199681},
journal = {PLoS ONE},
number = 6,
volume = 13,
place = {United States},
year = {2018},
month = {6}
}

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    Works referencing / citing this record:

    RNA-Seq analysis of Clerodendrum inerme (L.) roots in response to salt stress
    journal, October 2019