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Title: Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast

Abstract

Corn stover and sugarcane bagasse are the most widely available agriculture processing biomass and could serve as feedstocks for production of biofuel. In this study, three different technologies are combined to develop a more efficient conversion process for each of these feedstocks. The three technologies are diluted alkaline deacetylation process, combined thermochemical and mechanical shear pretreatment, and fermentation using a combined inoculum of two commercial Saccharomyces yeast strains. The two yeast strains used were a non-GMO and GMO strain engineered for xylose fermentation. The final ethanol concentrations obtained were 35.7 g/L from deacetylated corn stover and 32.9 g/L from sugarcane bagasse. Blending the two yeast reduced residual xylose content from 1.24 g/L to 0.48g/L and increased ethanol production by 6.5% compared to solely using the C5/C6 yeast. In conclusion, the optimized yeast blend also lowered the amount of C5/C6 yeast required for inoculation by 80%.

Authors:
 [1];  [2];  [1];  [1];  [1]
  1. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
  2. National Center for Agricultural Utilization Research, USDA, Peoria, IL (United States)
Publication Date:
Research Org.:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1498057
Alternate Identifier(s):
OSTI ID: 1547670
Grant/Contract Number:  
SC0018420
Resource Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 282; Journal Issue: C; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; corn stover; sugarcane bagasse; deacetylation; bioethanol; mixed yeast culture; two-stage pretreatment

Citation Formats

Wang, Zhaoqin, Dien, Bruce S., Rausch, Kent D., Tumbleson, M. E., and Singh, Vijay. Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast. United States: N. p., 2019. Web. doi:10.1016/j.biortech.2019.02.123.
Wang, Zhaoqin, Dien, Bruce S., Rausch, Kent D., Tumbleson, M. E., & Singh, Vijay. Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast. United States. doi:10.1016/j.biortech.2019.02.123.
Wang, Zhaoqin, Dien, Bruce S., Rausch, Kent D., Tumbleson, M. E., and Singh, Vijay. Fri . "Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast". United States. doi:10.1016/j.biortech.2019.02.123.
@article{osti_1498057,
title = {Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast},
author = {Wang, Zhaoqin and Dien, Bruce S. and Rausch, Kent D. and Tumbleson, M. E. and Singh, Vijay},
abstractNote = {Corn stover and sugarcane bagasse are the most widely available agriculture processing biomass and could serve as feedstocks for production of biofuel. In this study, three different technologies are combined to develop a more efficient conversion process for each of these feedstocks. The three technologies are diluted alkaline deacetylation process, combined thermochemical and mechanical shear pretreatment, and fermentation using a combined inoculum of two commercial Saccharomyces yeast strains. The two yeast strains used were a non-GMO and GMO strain engineered for xylose fermentation. The final ethanol concentrations obtained were 35.7 g/L from deacetylated corn stover and 32.9 g/L from sugarcane bagasse. Blending the two yeast reduced residual xylose content from 1.24 g/L to 0.48g/L and increased ethanol production by 6.5% compared to solely using the C5/C6 yeast. In conclusion, the optimized yeast blend also lowered the amount of C5/C6 yeast required for inoculation by 80%.},
doi = {10.1016/j.biortech.2019.02.123},
journal = {Bioresource Technology},
number = C,
volume = 282,
place = {United States},
year = {2019},
month = {3}
}

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This content will become publicly available on March 1, 2020
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