Glycosylation Is Vital for Industrial Performance of Hyperactive Cellulases

Chung, Daehwan; Sarai, Nicholas S.; Knott, Brandon C.; Hengge, Neal; Russell, Jordan F.; Yarbrough, John M.; Brunecky, Roman; Young, Jenna; Supekar, Nitin; Vander Wall, Todd; Sammond, Deanne W.; Crowley, Michael F.; Szymanski, Christine M.; Wells, Lance; Azadi, Parastoo; Westpheling, Janet; Himmel, Michael E.; Bomble, Yannick J.

doi:10.1021/acssuschemeng.8b05049

Title: Glycosylation Is Vital for Industrial Performance of Hyperactive Cellulases

Journal Article · 01 February 2019 · ACS Sustainable Chemistry & Engineering

DOI: https://doi.org/10.1021/acssuschemeng.8b05049 · OSTI ID:1497985

Chung, Daehwan ^[1]; Sarai, Nicholas S. ^[2]; Knott, Brandon C. ^[1]; Hengge, Neal ^[1]; Russell, Jordan F. ^[3]; Yarbrough, John M. ^[1]; Brunecky, Roman ^[1]; Young, Jenna ^[3]; Supekar, Nitin ^[3]; Vander Wall, Todd ^[1];

^[1];

^[1]; Szymanski, Christine M. ^[3]; Wells, Lance ^[3]; Azadi, Parastoo ^[3]; Westpheling, Janet ^[3]; Himmel, Michael E. ^[1];

^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States); California Inst. of Technology (CalTech), Pasadena, CA (United States)
Univ. of Georgia, Athens, GA (United States)

In the terrestrial biosphere, biomass deconstruction is conducted by microbes employing a variety of complementary strategies, many of which remain to be discovered. Moreover, the biofuels industry seeks more efficient (and less costly) cellulase formulations upon which to launch the nascent sustainable bioenergy economy. The glycan decoration of fungal cellulases has been shown to protect these enzymes from protease action and to enhance binding to cellulose. We show here that thermal tolerant bacterial cellulases are glycosylated as well, although the types and extents of decoration differ from their Eukaryotic counterparts. Our major findings are that glycosylation of CelA is uniform across its three linker peptides and composed of mainly galactose disaccharides (which is unique) and that this glycosylation dramatically impacts the hydrolysis of insoluble substrates, proteolytic and thermal stability, and substrate binding and changes the dynamics of the enzyme. This study suggests that the glycosylation of CelA is crucial for its exceptionally high cellulolytic activity on biomass and provides the robustness needed for this enzyme to function in harsh environments including industrial settings.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1497985

Report Number(s):: NREL/JA-2700-73100

Journal Information:: ACS Sustainable Chemistry & Engineering, Vol. 7, Issue 5; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 16 works

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Title: Glycosylation Is Vital for Industrial Performance of Hyperactive Cellulases

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