Characterization of a leukocidin identified in Staphylococcus pseudintermedius

Abouelkhair, Mohamed A.; Bemis, David A.; Giannone, Richard J.; Frank, Linda A.; Kania, Stephen A.

doi:10.1371/journal.pone.0204450

Title: Characterization of a leukocidin identified in Staphylococcus pseudintermedius

Journal Article · 26 September 2018 · PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0204450 · OSTI ID:1495979

^[1]; Bemis, David A. ^[1]; Giannone, Richard J. ^[2]; Frank, Linda A. ^[1];

^[1]

Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Bacterial infections from Staphylococcus pseudintermedius are the most common cause of skin infections (pyoderma) affecting dogs. Two component pore-forming leukocidins are a family of potent toxins secreted by staphylococci and consist of S (slow) and F (fast) components. They impair the innate immune system, the first line of defense against these pathogens. Seven different leukocidins have been characterized in Staphylococcus aureus, some of which are host and cell specific. Through genome sequencing and analysis of the S. pseudintermedius secretome using liquid chromatography mass spectrometry we identified two proteins, named “LukS-I” and “LukF-I”, encoded on a degenerate prophage contained in the genome of S. pseudintermedius isolates. Phylogenetic analysis of LukS-I components in comparison to the rest of the leukocidin family showed that LukS-I was most closely related to S. intermedius LukS-I, S. aureus LukE and LukP, whereas LukF-I was most similar to S. intermedius LukF-I S. aureus gamma hemolysin subunit B. The killing effect of recombinant S. pseudintermedius LukS-I and LukF-I on canine polymorphonuclear leukocytes was determined using a flow cytometry cell permeability assay. The cytotoxic effect occurred only when the two recombinant proteins were combined. Engineered mutant versions of the two-component pore-forming leukocidins, produced through amino acids substitutions at selected points, were not cytotoxic. Anti-Luk-I produced in dogs against attenuated proteins reduced the cytotoxic effect of native canine leukotoxin which highlights the importance of Luk-I as a promising component in a vaccine against canine S. pseudintermedius infections.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1495979

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 9 Vol. 13; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Characterization of New Staphylococcal Cassette Chromosome mec (SCCmec) and Topoisomerase Genes in Fluoroquinolone- and Methicillin-Resistant Staphylococcus pseudintermedius Descloux, S.; Rossano, A.; Perreten, V. Journal of Clinical Microbiology, Vol. 46, Issue 5 https://doi.org/10.1128/JCM.02255-07	journal	February 2008
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Structurally Designed Attenuated Subunit Vaccines for S. aureus LukS-PV and LukF-PV Confer Protection in a Mouse Bacteremia Model Karauzum, Hatice; Adhikari, Rajan P.; Sarwar, Jawad PLoS ONE, Vol. 8, Issue 6 https://doi.org/10.1371/journal.pone.0065384	journal	June 2013
Antibodies to S. aureus LukS-PV Attenuated Subunit Vaccine Neutralize a Broad Spectrum of Canonical and Non-Canonical Bicomponent Leukotoxin Pairs Adhikari, Rajan P.; Kort, Thomas; Shulenin, Sergey PLOS ONE, Vol. 10, Issue 9 https://doi.org/10.1371/journal.pone.0137874	journal	September 2015
Staphylococcus aureus Panton-Valentine Leukocidin Is a Very Potent Cytotoxic Factor for Human Neutrophils Löffler, Bettina; Hussain, Muzaffar; Grundmeier, Matthias PLoS Pathogens, Vol. 6, Issue 1 https://doi.org/10.1371/journal.ppat.1000715	journal	January 2010
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Characterization of new staphylococcal cassette chromosome mec (SCCmec) and topoisomerase genes in fluoroquinolone- and methicillin-resistant Staphylococcus pseudintermedius Rossano, Alexandra; Descloux, Sybill; Perreten, Vincent American Society for Microbiology https://doi.org/10.7892/boris.52020	text	January 2008

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