Characterization of a leukocidin identified in Staphylococcus pseudintermedius
Abstract
Bacterial infections from Staphylococcus pseudintermedius are the most common cause of skin infections (pyoderma) affecting dogs. Two component pore-forming leukocidins are a family of potent toxins secreted by staphylococci and consist of S (slow) and F (fast) components. They impair the innate immune system, the first line of defense against these pathogens. Seven different leukocidins have been characterized in Staphylococcus aureus, some of which are host and cell specific. Through genome sequencing and analysis of the S. pseudintermedius secretome using liquid chromatography mass spectrometry we identified two proteins, named “LukS-I” and “LukF-I”, encoded on a degenerate prophage contained in the genome of S. pseudintermedius isolates. Phylogenetic analysis of LukS-I components in comparison to the rest of the leukocidin family showed that LukS-I was most closely related to S. intermedius LukS-I, S. aureus LukE and LukP, whereas LukF-I was most similar to S. intermedius LukF-I S. aureus gamma hemolysin subunit B. The killing effect of recombinant S. pseudintermedius LukS-I and LukF-I on canine polymorphonuclear leukocytes was determined using a flow cytometry cell permeability assay. The cytotoxic effect occurred only when the two recombinant proteins were combined. Engineered mutant versions of the two-component pore-forming leukocidins, produced through amino acids substitutions at selectedmore »
- Authors:
-
[1];
[1]
- Univ. of Tennessee, Knoxville, TN (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1495979
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 13; Journal Issue: 9; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Abouelkhair, Mohamed A., Bemis, David A., Giannone, Richard J., Frank, Linda A., and Kania, Stephen A. Characterization of a leukocidin identified in Staphylococcus pseudintermedius. United States: N. p., 2018.
Web. doi:10.1371/journal.pone.0204450.
Abouelkhair, Mohamed A., Bemis, David A., Giannone, Richard J., Frank, Linda A., & Kania, Stephen A. Characterization of a leukocidin identified in Staphylococcus pseudintermedius. United States. https://doi.org/10.1371/journal.pone.0204450
Abouelkhair, Mohamed A., Bemis, David A., Giannone, Richard J., Frank, Linda A., and Kania, Stephen A. Thu .
"Characterization of a leukocidin identified in Staphylococcus pseudintermedius". United States. https://doi.org/10.1371/journal.pone.0204450. https://www.osti.gov/servlets/purl/1495979.
@article{osti_1495979,
title = {Characterization of a leukocidin identified in Staphylococcus pseudintermedius},
author = {Abouelkhair, Mohamed A. and Bemis, David A. and Giannone, Richard J. and Frank, Linda A. and Kania, Stephen A.},
abstractNote = {Bacterial infections from Staphylococcus pseudintermedius are the most common cause of skin infections (pyoderma) affecting dogs. Two component pore-forming leukocidins are a family of potent toxins secreted by staphylococci and consist of S (slow) and F (fast) components. They impair the innate immune system, the first line of defense against these pathogens. Seven different leukocidins have been characterized in Staphylococcus aureus, some of which are host and cell specific. Through genome sequencing and analysis of the S. pseudintermedius secretome using liquid chromatography mass spectrometry we identified two proteins, named “LukS-I” and “LukF-I”, encoded on a degenerate prophage contained in the genome of S. pseudintermedius isolates. Phylogenetic analysis of LukS-I components in comparison to the rest of the leukocidin family showed that LukS-I was most closely related to S. intermedius LukS-I, S. aureus LukE and LukP, whereas LukF-I was most similar to S. intermedius LukF-I S. aureus gamma hemolysin subunit B. The killing effect of recombinant S. pseudintermedius LukS-I and LukF-I on canine polymorphonuclear leukocytes was determined using a flow cytometry cell permeability assay. The cytotoxic effect occurred only when the two recombinant proteins were combined. Engineered mutant versions of the two-component pore-forming leukocidins, produced through amino acids substitutions at selected points, were not cytotoxic. Anti-Luk-I produced in dogs against attenuated proteins reduced the cytotoxic effect of native canine leukotoxin which highlights the importance of Luk-I as a promising component in a vaccine against canine S. pseudintermedius infections.},
doi = {10.1371/journal.pone.0204450},
journal = {PLoS ONE},
number = 9,
volume = 13,
place = {United States},
year = {Thu Sep 27 00:00:00 EDT 2018},
month = {Thu Sep 27 00:00:00 EDT 2018}
}
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The Phyre2 web portal for protein modeling, prediction and analysis
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Antibodies to S. aureus LukS-PV Attenuated Subunit Vaccine Neutralize a Broad Spectrum of Canonical and Non-Canonical Bicomponent Leukotoxin Pairs
journal, September 2015
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Staphylococcus aureus Panton-Valentine Leukocidin Is a Very Potent Cytotoxic Factor for Human Neutrophils
journal, January 2010
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The potential use of toxin antibodies as a strategy for controlling acute Staphylococcus aureus infections
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Works referencing / citing this record:
Identification, cloning and characterization of SpEX exotoxin produced by Staphylococcus pseudintermedius
journal, July 2019
- Abouelkhair, Mohamed A.; Bemis, David A.; Giannone, Richard J.
- PLOS ONE, Vol. 14, Issue 7
Underrated Staphylococcus species and their role in antimicrobial resistance spreading
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Identification, cloning and characterization of SpEX exotoxin produced by Staphylococcus pseudintermedius
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- Abouelkhair, Mohamed A.; Bemis, David A.; Giannone, Richard J.
- PLOS ONE, Vol. 14, Issue 7