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Title: The Macromolecular Femtosecond Crystallography Instrument at the Linac Coherent Light Source

Abstract

The Macromolecular Femtosecond Crystallography (MFX) instrument at the Linac Coherent Light Source (LCLS) is the seventh and newest instrument at the world's first hard X-ray free-electron laser. It was designed with a primary focus on structural biology, employing the ultrafast pulses of X-rays from LCLS at atmospheric conditions to overcome radiation damage limitations in biological measurements. It is also capable of performing various time-resolved measurements. The MFX design consists of a versatile base system capable of supporting multiple methods, techniques and experimental endstations. The primary techniques supported are forward scattering and crystallography, with capabilities for various spectroscopic methods and time-resolved measurements. The location of the MFX instrument allows for utilization of multiplexing methods, increasing user access to LCLS by running multiple experiments simultaneously.

Authors:
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Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Nuclear Physics (NP) (SC-26); National Science Foundation (NSF)
OSTI Identifier:
1495876
Alternate Identifier(s):
OSTI ID: 1505631; OSTI ID: 1514708; OSTI ID: 1605922
Report Number(s):
BNL-211684-2019-JAAM
Journal ID: ISSN 1600-5775; JSYRES; PII: S1600577519001577
Grant/Contract Number:  
AC02-76SF00515; SC0012704
Resource Type:
Published Article
Journal Name:
Journal of Synchrotron Radiation
Additional Journal Information:
Journal Name: Journal of Synchrotron Radiation Journal Volume: 26 Journal Issue: 2; Journal ID: ISSN 1600-5775
Publisher:
International Union of Crystallography (IUCr)
Country of Publication:
Denmark
Language:
English
Subject:
47 OTHER INSTRUMENTATION; X-ray free-electron laser; serial femtosecond crystallography; protein crystallography; time-resolved crystallography; room-temperature crystallography; X-ray FEL; 73 NUCLEAR PHYSICS AND RADIATION PHYSICS; 38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY

Citation Formats

Sierra, Raymond G., Batyuk, Alexander, Sun, Zhibin, Aquila, Andrew, Hunter, Mark S., Lane, Thomas J., Liang, Mengning, Yoon, Chun Hong, Alonso-Mori, Roberto, Armenta, Rebecca, Castagna, Jean-Charles, Hollenbeck, Michael, Osier, Ted O., Hayes, Matt, Aldrich, Jeff, Curtis, Robin, Koglin, Jason E., Rendahl, Theodore, Rodriguez, Evan, Carbajo, Sergio, Guillet, Serge, Paul, Rob, Hart, Philip, Nakahara, Kazutaka, Carini, Gabriella, DeMirci, Hasan, Dao, E. Han, Hayes, Brandon M., Rao, Yashas P., Chollet, Matthieu, Feng, Yiping, Fuller, Franklin D., Kupitz, Christopher, Sato, Takahiro, Seaberg, Matthew H., Song, Sanghoon, van Driel, Tim B., Yavas, Hasan, Zhu, Diling, Cohen, Aina E., Wakatsuki, Soichi, and Boutet, Sébastien. The Macromolecular Femtosecond Crystallography Instrument at the Linac Coherent Light Source. Denmark: N. p., 2019. Web. doi:10.1107/S1600577519001577.
Sierra, Raymond G., Batyuk, Alexander, Sun, Zhibin, Aquila, Andrew, Hunter, Mark S., Lane, Thomas J., Liang, Mengning, Yoon, Chun Hong, Alonso-Mori, Roberto, Armenta, Rebecca, Castagna, Jean-Charles, Hollenbeck, Michael, Osier, Ted O., Hayes, Matt, Aldrich, Jeff, Curtis, Robin, Koglin, Jason E., Rendahl, Theodore, Rodriguez, Evan, Carbajo, Sergio, Guillet, Serge, Paul, Rob, Hart, Philip, Nakahara, Kazutaka, Carini, Gabriella, DeMirci, Hasan, Dao, E. Han, Hayes, Brandon M., Rao, Yashas P., Chollet, Matthieu, Feng, Yiping, Fuller, Franklin D., Kupitz, Christopher, Sato, Takahiro, Seaberg, Matthew H., Song, Sanghoon, van Driel, Tim B., Yavas, Hasan, Zhu, Diling, Cohen, Aina E., Wakatsuki, Soichi, & Boutet, Sébastien. The Macromolecular Femtosecond Crystallography Instrument at the Linac Coherent Light Source. Denmark. doi:10.1107/S1600577519001577.
Sierra, Raymond G., Batyuk, Alexander, Sun, Zhibin, Aquila, Andrew, Hunter, Mark S., Lane, Thomas J., Liang, Mengning, Yoon, Chun Hong, Alonso-Mori, Roberto, Armenta, Rebecca, Castagna, Jean-Charles, Hollenbeck, Michael, Osier, Ted O., Hayes, Matt, Aldrich, Jeff, Curtis, Robin, Koglin, Jason E., Rendahl, Theodore, Rodriguez, Evan, Carbajo, Sergio, Guillet, Serge, Paul, Rob, Hart, Philip, Nakahara, Kazutaka, Carini, Gabriella, DeMirci, Hasan, Dao, E. Han, Hayes, Brandon M., Rao, Yashas P., Chollet, Matthieu, Feng, Yiping, Fuller, Franklin D., Kupitz, Christopher, Sato, Takahiro, Seaberg, Matthew H., Song, Sanghoon, van Driel, Tim B., Yavas, Hasan, Zhu, Diling, Cohen, Aina E., Wakatsuki, Soichi, and Boutet, Sébastien. Fri . "The Macromolecular Femtosecond Crystallography Instrument at the Linac Coherent Light Source". Denmark. doi:10.1107/S1600577519001577.
@article{osti_1495876,
title = {The Macromolecular Femtosecond Crystallography Instrument at the Linac Coherent Light Source},
author = {Sierra, Raymond G. and Batyuk, Alexander and Sun, Zhibin and Aquila, Andrew and Hunter, Mark S. and Lane, Thomas J. and Liang, Mengning and Yoon, Chun Hong and Alonso-Mori, Roberto and Armenta, Rebecca and Castagna, Jean-Charles and Hollenbeck, Michael and Osier, Ted O. and Hayes, Matt and Aldrich, Jeff and Curtis, Robin and Koglin, Jason E. and Rendahl, Theodore and Rodriguez, Evan and Carbajo, Sergio and Guillet, Serge and Paul, Rob and Hart, Philip and Nakahara, Kazutaka and Carini, Gabriella and DeMirci, Hasan and Dao, E. Han and Hayes, Brandon M. and Rao, Yashas P. and Chollet, Matthieu and Feng, Yiping and Fuller, Franklin D. and Kupitz, Christopher and Sato, Takahiro and Seaberg, Matthew H. and Song, Sanghoon and van Driel, Tim B. and Yavas, Hasan and Zhu, Diling and Cohen, Aina E. and Wakatsuki, Soichi and Boutet, Sébastien},
abstractNote = {The Macromolecular Femtosecond Crystallography (MFX) instrument at the Linac Coherent Light Source (LCLS) is the seventh and newest instrument at the world's first hard X-ray free-electron laser. It was designed with a primary focus on structural biology, employing the ultrafast pulses of X-rays from LCLS at atmospheric conditions to overcome radiation damage limitations in biological measurements. It is also capable of performing various time-resolved measurements. The MFX design consists of a versatile base system capable of supporting multiple methods, techniques and experimental endstations. The primary techniques supported are forward scattering and crystallography, with capabilities for various spectroscopic methods and time-resolved measurements. The location of the MFX instrument allows for utilization of multiplexing methods, increasing user access to LCLS by running multiple experiments simultaneously.},
doi = {10.1107/S1600577519001577},
journal = {Journal of Synchrotron Radiation},
number = 2,
volume = 26,
place = {Denmark},
year = {2019},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1107/S1600577519001577

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Cited by: 4 works
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Figures / Tables:

Table 1 Table 1: X-ray parameters and capabilities of the MFX instrument.

Existing LCLS parameters are derived from Emma et al. (2010), Amann et al. (2012), Bostedt et al. (2016) and Liu et al. (2018). LCLS-II capabilities are extracted from Galayda (2014).


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Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.