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Title: Does co-transcriptional regulation of alternative splicing mediate plant stress responses?

Abstract

Plants display exquisite control over gene expression to elicit appropriate responses under normal and stress conditions. Alternative splicing (AS) of pre-mRNAs, a process that generates two or more transcripts from multi-exon genes, adds another layer of regulation to fine-tune condition-specific gene expression in animals and plants. However, exactly how plants control splice isoform ratios and the timing of this regulation in response to environmental signals remains elusive. In mammals, recent evidence indicate that epigenetic and epitranscriptome changes, such as DNA methylation, chromatin modifications and RNA methylation, regulate RNA polymerase II processivity, co-transcriptional splicing, and stability and translation efficiency of splice isoforms. In plants, the role of epigenetic modifications in regulating transcription rate and mRNA abundance under stress is beginning to emerge. However, the mechanisms by which epigenetic and epitranscriptomic modifications regulate AS and translation efficiency require further research. Dynamic changes in the chromatin landscape in response to stress may provide a scaffold around which gene expression, AS and translation are orchestrated. Finally, we discuss CRISPR/Cas-based strategies for engineering chromatin architecture to manipulate AS patterns (or splice isoforms levels) to obtain insight into the epigenetic regulation of AS.

Authors:
 [1];  [2]; ORCiD logo [3];  [1];  [1];  [1]; ORCiD logo [1]; ORCiD logo [1]
  1. School of Human and Life Sciences, Canterbury Christ Church University, Canterbury, CT1 1QU, UK
  2. Department of Biology and Program in Cell and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1878, USA
  3. Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences - BOKU, Muthgasse 18, 1190 Vienna, Austria
Publication Date:
Research Org.:
Colorado State Univ., Fort Collins, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1495855
Alternate Identifier(s):
OSTI ID: 1611510
Grant/Contract Number:  
SC0010733
Resource Type:
Published Article
Journal Name:
Nucleic Acids Research
Additional Journal Information:
Journal Name: Nucleic Acids Research Journal Volume: 47 Journal Issue: 6; Journal ID: ISSN 0305-1048
Publisher:
Oxford University Press
Country of Publication:
United Kingdom
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 54 ENVIRONMENTAL SCIENCES; Biochemistry & Molecular Biology

Citation Formats

Jabre, Ibtissam, Reddy, Anireddy S. N., Kalyna, Maria, Chaudhary, Saurabh, Khokhar, Waqas, Byrne, Lee J., Wilson, Cornelia M., and Syed, Naeem H. Does co-transcriptional regulation of alternative splicing mediate plant stress responses?. United Kingdom: N. p., 2019. Web. https://doi.org/10.1093/nar/gkz121.
Jabre, Ibtissam, Reddy, Anireddy S. N., Kalyna, Maria, Chaudhary, Saurabh, Khokhar, Waqas, Byrne, Lee J., Wilson, Cornelia M., & Syed, Naeem H. Does co-transcriptional regulation of alternative splicing mediate plant stress responses?. United Kingdom. https://doi.org/10.1093/nar/gkz121
Jabre, Ibtissam, Reddy, Anireddy S. N., Kalyna, Maria, Chaudhary, Saurabh, Khokhar, Waqas, Byrne, Lee J., Wilson, Cornelia M., and Syed, Naeem H. Fri . "Does co-transcriptional regulation of alternative splicing mediate plant stress responses?". United Kingdom. https://doi.org/10.1093/nar/gkz121.
@article{osti_1495855,
title = {Does co-transcriptional regulation of alternative splicing mediate plant stress responses?},
author = {Jabre, Ibtissam and Reddy, Anireddy S. N. and Kalyna, Maria and Chaudhary, Saurabh and Khokhar, Waqas and Byrne, Lee J. and Wilson, Cornelia M. and Syed, Naeem H.},
abstractNote = {Plants display exquisite control over gene expression to elicit appropriate responses under normal and stress conditions. Alternative splicing (AS) of pre-mRNAs, a process that generates two or more transcripts from multi-exon genes, adds another layer of regulation to fine-tune condition-specific gene expression in animals and plants. However, exactly how plants control splice isoform ratios and the timing of this regulation in response to environmental signals remains elusive. In mammals, recent evidence indicate that epigenetic and epitranscriptome changes, such as DNA methylation, chromatin modifications and RNA methylation, regulate RNA polymerase II processivity, co-transcriptional splicing, and stability and translation efficiency of splice isoforms. In plants, the role of epigenetic modifications in regulating transcription rate and mRNA abundance under stress is beginning to emerge. However, the mechanisms by which epigenetic and epitranscriptomic modifications regulate AS and translation efficiency require further research. Dynamic changes in the chromatin landscape in response to stress may provide a scaffold around which gene expression, AS and translation are orchestrated. Finally, we discuss CRISPR/Cas-based strategies for engineering chromatin architecture to manipulate AS patterns (or splice isoforms levels) to obtain insight into the epigenetic regulation of AS.},
doi = {10.1093/nar/gkz121},
journal = {Nucleic Acids Research},
number = 6,
volume = 47,
place = {United Kingdom},
year = {2019},
month = {2}
}

Journal Article:
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https://doi.org/10.1093/nar/gkz121

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    Works referencing / citing this record:

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