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Title: Direct Writing of Tunable Living Inks for Bioprocess Intensification

Abstract

Critical to the success of three-dimensional (3D) printing of living materials with high performance is the development of new ink materials and 3D geometries that favor long-term cell functionality. Here we report the use of freeze-dried live cells as the solid filler to enable a new living material system for direct ink writing of catalytically active microorganisms with tunable densities and various self-supporting porous 3D geometries. Baker’s yeast was used as an exemplary live whole-cell biocatalyst, and the printed structures displayed high resolution, large scale, high catalytic activity and long-term viability. An unprecedented high cell loading was achieved, and cell inks showed unique thixotropic behavior. In the presence of glucose, printed bioscaffolds exhibited increased ethanol production compared to bulk counterparts due largely to improved mass transfer through engineered porous structures. The new living materials developed in this work could serve as a versatile platform for process intensification of an array of bioconversion processes utilizing diverse microbial biocatalysts for production of high-value products or bioremediation applications.

Authors:
ORCiD logo [1];  [1]; ORCiD logo [1];  [1];  [1];  [2];  [1];  [1];  [3];  [3];  [1]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  2. Univ. of California, Davis, CA (United States)
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1495251
Alternate Identifier(s):
OSTI ID: 1508842
Grant/Contract Number:  
AC52-07NA27344; Lab-directed research and development/17-FS-027; Lab-directed research and development/19-ERD-005
Resource Type:
Published Article
Journal Name:
Nano Letters
Additional Journal Information:
Journal Name: Nano Letters; Journal ID: ISSN 1530-6984
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; additive manufacturing; biocatalysts; bioinks; Bioprinting; living materials

Citation Formats

Qian, Fang, Zhu, Cheng, Knipe, Jennifer M., Ruelas, Samantha, Stolaroff, Joshuah K., DeOtte, Joshua R., Duoss, Eric B., Spadaccini, Christopher M., Henard, Calvin A., Guarnieri, Michael T., and Baker, Sarah E. Direct Writing of Tunable Living Inks for Bioprocess Intensification. United States: N. p., 2019. Web. doi:10.1021/acs.nanolett.9b00066.
Qian, Fang, Zhu, Cheng, Knipe, Jennifer M., Ruelas, Samantha, Stolaroff, Joshuah K., DeOtte, Joshua R., Duoss, Eric B., Spadaccini, Christopher M., Henard, Calvin A., Guarnieri, Michael T., & Baker, Sarah E. Direct Writing of Tunable Living Inks for Bioprocess Intensification. United States. doi:10.1021/acs.nanolett.9b00066.
Qian, Fang, Zhu, Cheng, Knipe, Jennifer M., Ruelas, Samantha, Stolaroff, Joshuah K., DeOtte, Joshua R., Duoss, Eric B., Spadaccini, Christopher M., Henard, Calvin A., Guarnieri, Michael T., and Baker, Sarah E. Thu . "Direct Writing of Tunable Living Inks for Bioprocess Intensification". United States. doi:10.1021/acs.nanolett.9b00066.
@article{osti_1495251,
title = {Direct Writing of Tunable Living Inks for Bioprocess Intensification},
author = {Qian, Fang and Zhu, Cheng and Knipe, Jennifer M. and Ruelas, Samantha and Stolaroff, Joshuah K. and DeOtte, Joshua R. and Duoss, Eric B. and Spadaccini, Christopher M. and Henard, Calvin A. and Guarnieri, Michael T. and Baker, Sarah E.},
abstractNote = {Critical to the success of three-dimensional (3D) printing of living materials with high performance is the development of new ink materials and 3D geometries that favor long-term cell functionality. Here we report the use of freeze-dried live cells as the solid filler to enable a new living material system for direct ink writing of catalytically active microorganisms with tunable densities and various self-supporting porous 3D geometries. Baker’s yeast was used as an exemplary live whole-cell biocatalyst, and the printed structures displayed high resolution, large scale, high catalytic activity and long-term viability. An unprecedented high cell loading was achieved, and cell inks showed unique thixotropic behavior. In the presence of glucose, printed bioscaffolds exhibited increased ethanol production compared to bulk counterparts due largely to improved mass transfer through engineered porous structures. The new living materials developed in this work could serve as a versatile platform for process intensification of an array of bioconversion processes utilizing diverse microbial biocatalysts for production of high-value products or bioremediation applications.},
doi = {10.1021/acs.nanolett.9b00066},
journal = {Nano Letters},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1021/acs.nanolett.9b00066

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