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Title: Direct Writing of Tunable Living Inks for Bioprocess Intensification

Abstract

Critical to the success of three-dimensional (3D) printing of living materials with high performance is the development of new ink materials and 3D geometries that favor long-term cell functionality. Here we report the use of freeze-dried live cells as the solid filler to enable a new living material system for direct ink writing of catalytically active microorganisms with tunable densities and various self-supporting porous 3D geometries. Baker’s yeast was used as an exemplary live whole-cell biocatalyst, and the printed structures displayed high resolution, large scale, high catalytic activity and long-term viability. An unprecedented high cell loading was achieved, and cell inks showed unique thixotropic behavior. In the presence of glucose, printed bioscaffolds exhibited increased ethanol production compared to bulk counterparts due largely to improved mass transfer through engineered porous structures. The new living materials developed in this work could serve as a versatile platform for process intensification of an array of bioconversion processes utilizing diverse microbial biocatalysts for production of high-value products or bioremediation applications.

Authors:
ORCiD logo; ; ORCiD logo; ; ;  [1]; ; ;  [2];  [2];
  1. University of California, Davis, California 95616, United States
  2. National Bioenergy Center, National Renewable Energy Laboratory, Golden, Colorado 80401, United States
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE); USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1495251
Alternate Identifier(s):
OSTI ID: 1500077; OSTI ID: 1508842; OSTI ID: 1592032
Report Number(s):
NREL/JA-5100-73418; LLNL-JRNL-753559
Journal ID: ISSN 1530-6984
Grant/Contract Number:  
AC36-08GO28308; AC52-07NA27344
Resource Type:
Published Article
Journal Name:
Nano Letters
Additional Journal Information:
Journal Name: Nano Letters Journal Volume: 19 Journal Issue: 9; Journal ID: ISSN 1530-6984
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; additive manufacturing; biocatalysts; bioinks; bioprinting; living materials; 36 MATERIALS SCIENCE

Citation Formats

Qian, Fang, Zhu, Cheng, Knipe, Jennifer M., Ruelas, Samantha, Stolaroff, Joshuah K., DeOtte, Joshua R., Duoss, Eric B., Spadaccini, Christopher M., Henard, Calvin A., Guarnieri, Michael T., and Baker, Sarah E. Direct Writing of Tunable Living Inks for Bioprocess Intensification. United States: N. p., 2019. Web. doi:10.1021/acs.nanolett.9b00066.
Qian, Fang, Zhu, Cheng, Knipe, Jennifer M., Ruelas, Samantha, Stolaroff, Joshuah K., DeOtte, Joshua R., Duoss, Eric B., Spadaccini, Christopher M., Henard, Calvin A., Guarnieri, Michael T., & Baker, Sarah E. Direct Writing of Tunable Living Inks for Bioprocess Intensification. United States. doi:10.1021/acs.nanolett.9b00066.
Qian, Fang, Zhu, Cheng, Knipe, Jennifer M., Ruelas, Samantha, Stolaroff, Joshuah K., DeOtte, Joshua R., Duoss, Eric B., Spadaccini, Christopher M., Henard, Calvin A., Guarnieri, Michael T., and Baker, Sarah E. Thu . "Direct Writing of Tunable Living Inks for Bioprocess Intensification". United States. doi:10.1021/acs.nanolett.9b00066.
@article{osti_1495251,
title = {Direct Writing of Tunable Living Inks for Bioprocess Intensification},
author = {Qian, Fang and Zhu, Cheng and Knipe, Jennifer M. and Ruelas, Samantha and Stolaroff, Joshuah K. and DeOtte, Joshua R. and Duoss, Eric B. and Spadaccini, Christopher M. and Henard, Calvin A. and Guarnieri, Michael T. and Baker, Sarah E.},
abstractNote = {Critical to the success of three-dimensional (3D) printing of living materials with high performance is the development of new ink materials and 3D geometries that favor long-term cell functionality. Here we report the use of freeze-dried live cells as the solid filler to enable a new living material system for direct ink writing of catalytically active microorganisms with tunable densities and various self-supporting porous 3D geometries. Baker’s yeast was used as an exemplary live whole-cell biocatalyst, and the printed structures displayed high resolution, large scale, high catalytic activity and long-term viability. An unprecedented high cell loading was achieved, and cell inks showed unique thixotropic behavior. In the presence of glucose, printed bioscaffolds exhibited increased ethanol production compared to bulk counterparts due largely to improved mass transfer through engineered porous structures. The new living materials developed in this work could serve as a versatile platform for process intensification of an array of bioconversion processes utilizing diverse microbial biocatalysts for production of high-value products or bioremediation applications.},
doi = {10.1021/acs.nanolett.9b00066},
journal = {Nano Letters},
number = 9,
volume = 19,
place = {United States},
year = {2019},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1021/acs.nanolett.9b00066

Citation Metrics:
Cited by: 12 works
Citation information provided by
Web of Science

Figures / Tables:

Figure 1 Figure 1: Preparation and printing of bioinks with tunable cell loading. (a) Schematic of the ink components of filler(s), binder, and photoinitiator. (b) Photograph of the printing nozzle in the process of writing a 3D scaffold structure.

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Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.