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Title: Single cell genomic and transcriptomic evidence for the use of alternative nitrogen substrates by anammox bacteria

Abstract

Anaerobic ammonium oxidation (anammox) contributes substantially to ocean nitrogen loss, particularly in anoxic marine zones (AMZs). Ammonium is scarce in AMZs, raising the hypothesis that organic nitrogen compounds may be ammonium sources for anammox. Biochemical measurements suggest that the organic compounds urea and cyanate can support anammox in AMZs. However, it is unclear if anammox bacteria degrade these compounds to ammonium themselves, or rely on other organisms for this process. Genes for urea degradation have not been found in anammox bacteria, and genomic evidence for cyanate use for anammox is limited to a cyanase gene recovered from the sediment bacterium Candidatus Scalindua profunda. Here, analysis of Ca. Scalindua single amplified genomes from the Eastern Tropical North Pacific AMZ revealed genes for urea degradation and transport, as well as for cyanate degradation. Urease and cyanase genes were transcribed, along with anammox genes, in the AMZ core where anammox rates peaked. Homologs of these genes were also detected in meta-omic datasets from major AMZs in the Eastern Tropical South Pacific and Arabian Sea. These results suggest that anammox bacteria from different ocean regions can directly access organic nitrogen substrates. Future studies should assess if and under what environmental conditions these substrates contributemore » to the ammonium budget for anammox.« less

Authors:
ORCiD logo [1];  [2];  [3];  [2];  [4]; ORCiD logo [5];  [6];  [7];  [8];  [8];  [5];  [2]; ORCiD logo [9];  [2]
  1. Georgia Inst. of Technology, Atlanta, GA (United States); Radiant Genomics, Emeryville, CA (United States)
  2. Georgia Inst. of Technology, Atlanta, GA (United States)
  3. Max Planck Inst. for Marine Microbiology, Bremen (Germany)
  4. Univ. of Pennsylvania, Philadelphia, PA (United States)
  5. Univ. de Concepcion (Chile)
  6. Woods Hole Oceanographic Inst., Woods Hole, MA (United States); Univ. of Massachusetts Dartmouth, New Bedford, MA (United States)
  7. Univ. of Massachusetts Dartmouth, New Bedford, MA (United States)
  8. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  9. Univ. of Southern Denmark, Odense (Denmark)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1495047
Grant/Contract Number:  
[AC02-05CH11231]
Resource Type:
Accepted Manuscript
Journal Name:
The ISME Journal
Additional Journal Information:
[ Journal Volume: 12; Journal Issue: 11]; Journal ID: ISSN 1751-7362
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Ganesh, Sangita, Bertagnolli, Anthony D., Bristow, Laura A., Padilla, Cory C., Blackwood, Nigel, Aldunate, Montserrat, Bourbonnais, Annie, Altabet, Mark A., Malmstrom, Rex R., Woyke, Tanja, Ulloa, Osvaldo, Konstantinidis, Konstantinos T., Thamdrup, Bo, and Stewart, Frank J. Single cell genomic and transcriptomic evidence for the use of alternative nitrogen substrates by anammox bacteria. United States: N. p., 2018. Web. doi:10.1038/s41396-018-0223-9.
Ganesh, Sangita, Bertagnolli, Anthony D., Bristow, Laura A., Padilla, Cory C., Blackwood, Nigel, Aldunate, Montserrat, Bourbonnais, Annie, Altabet, Mark A., Malmstrom, Rex R., Woyke, Tanja, Ulloa, Osvaldo, Konstantinidis, Konstantinos T., Thamdrup, Bo, & Stewart, Frank J. Single cell genomic and transcriptomic evidence for the use of alternative nitrogen substrates by anammox bacteria. United States. doi:10.1038/s41396-018-0223-9.
Ganesh, Sangita, Bertagnolli, Anthony D., Bristow, Laura A., Padilla, Cory C., Blackwood, Nigel, Aldunate, Montserrat, Bourbonnais, Annie, Altabet, Mark A., Malmstrom, Rex R., Woyke, Tanja, Ulloa, Osvaldo, Konstantinidis, Konstantinos T., Thamdrup, Bo, and Stewart, Frank J. Tue . "Single cell genomic and transcriptomic evidence for the use of alternative nitrogen substrates by anammox bacteria". United States. doi:10.1038/s41396-018-0223-9. https://www.osti.gov/servlets/purl/1495047.
@article{osti_1495047,
title = {Single cell genomic and transcriptomic evidence for the use of alternative nitrogen substrates by anammox bacteria},
author = {Ganesh, Sangita and Bertagnolli, Anthony D. and Bristow, Laura A. and Padilla, Cory C. and Blackwood, Nigel and Aldunate, Montserrat and Bourbonnais, Annie and Altabet, Mark A. and Malmstrom, Rex R. and Woyke, Tanja and Ulloa, Osvaldo and Konstantinidis, Konstantinos T. and Thamdrup, Bo and Stewart, Frank J.},
abstractNote = {Anaerobic ammonium oxidation (anammox) contributes substantially to ocean nitrogen loss, particularly in anoxic marine zones (AMZs). Ammonium is scarce in AMZs, raising the hypothesis that organic nitrogen compounds may be ammonium sources for anammox. Biochemical measurements suggest that the organic compounds urea and cyanate can support anammox in AMZs. However, it is unclear if anammox bacteria degrade these compounds to ammonium themselves, or rely on other organisms for this process. Genes for urea degradation have not been found in anammox bacteria, and genomic evidence for cyanate use for anammox is limited to a cyanase gene recovered from the sediment bacterium Candidatus Scalindua profunda. Here, analysis of Ca. Scalindua single amplified genomes from the Eastern Tropical North Pacific AMZ revealed genes for urea degradation and transport, as well as for cyanate degradation. Urease and cyanase genes were transcribed, along with anammox genes, in the AMZ core where anammox rates peaked. Homologs of these genes were also detected in meta-omic datasets from major AMZs in the Eastern Tropical South Pacific and Arabian Sea. These results suggest that anammox bacteria from different ocean regions can directly access organic nitrogen substrates. Future studies should assess if and under what environmental conditions these substrates contribute to the ammonium budget for anammox.},
doi = {10.1038/s41396-018-0223-9},
journal = {The ISME Journal},
number = [11],
volume = [12],
place = {United States},
year = {2018},
month = {7}
}

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