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Title: Production of biofuels and chemicals from xylose using native and engineered yeast strains

Abstract

Numerous metabolic engineering strategies have allowed yeasts to efficiently assimilate xylose, the second most abundant sugar component of lignocellulosic biomass. During the investigation of xylose utilization by yeasts, a global rewiring of metabolic networks upon xylose cultivation has been captured, as opposed to a pattern of glucose repression. A clear understanding of the xylose-induced metabolic reprogramming in yeast would shed light on the optimization of yeast-based bioprocesses to produce biofuels and chemicals using xylose. In this review, we delved into characteristics of yeast xylose metabolism, and potential benefits of using xylose as a carbon source to produce various biochemicals with examples. Transcriptomic and metabolomic patterns of xylose-grown yeast cells were distinct from those on glucose—a conventional sugar of industrial biotechnology—and the gap might lead to opportunities to produce biochemicals efficiently. Indeed, limited glycolytic metabolic fluxes during xylose utilization could result in enhanced production of metabolites whose biosynthetic pathways compete for precursors with ethanol fermentation. Also, alleviation of glucose repression on cytosolic acetyl coenzyme A (acetyl-CoA) synthesis, and respiratory energy metabolism during xylose utilization enhanced production of acetyl-CoA derivatives. Consideration of singular properties of xylose metabolism, such as redox cofactor imbalance between xylose reductase and xylitol dehydrogenase, is necessary to maximizemore » these positive xylose effects. As a result, this review argues the importance and benefits of xylose utilization as not only a way of expanding a substrate range, but also an effective environmental perturbation for the efficient production of advanced biofuels and chemicals in yeasts.« less

Authors:
 [1];  [2];  [3];  [1]; ORCiD logo [2]
+ Show Author Affiliations
  1. Univ. of Illinois, Urbana-Champaign, IL (United States). Carl R. Woese Inst. for Genomic Biology
  2. Seoul National Univ. (Korea, Republic of). Dept. of Agricultural Biotechnology and Center for Food and Bioconvergence
  3. Univ. of Illinois, Urbana-Champaign, IL (United States). Carl R. Woese Inst. for Genomic Biology; Korea Univ., Seoul (South Korea). Dept. of Biotechnology, Graduate School
Publication Date:
Research Org.:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); Korea Institute for Industrial Economics and Trade (KIET)
OSTI Identifier:
1495021
Alternate Identifier(s):
OSTI ID: 1487444
Grant/Contract Number:  
SC0018420
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology Advances
Additional Journal Information:
Journal Volume: 37; Journal Issue: 2; Journal ID: ISSN 0734-9750
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Kwak, Suryang, Jo, Jung Hyun, Yun, Eun Ju, Jin, Yong-Su, and Seo, Jin-Ho. Production of biofuels and chemicals from xylose using native and engineered yeast strains. United States: N. p., 2019. Web. doi:10.1016/j.biotechadv.2018.12.003.
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Kwak, Suryang, Jo, Jung Hyun, Yun, Eun Ju, Jin, Yong-Su, & Seo, Jin-Ho. Production of biofuels and chemicals from xylose using native and engineered yeast strains. United States. https://doi.org/10.1016/j.biotechadv.2018.12.003
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Kwak, Suryang, Jo, Jung Hyun, Yun, Eun Ju, Jin, Yong-Su, and Seo, Jin-Ho. Fri . "Production of biofuels and chemicals from xylose using native and engineered yeast strains". United States. https://doi.org/10.1016/j.biotechadv.2018.12.003. https://www.osti.gov/servlets/purl/1495021.
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@article{osti_1495021,
title = {Production of biofuels and chemicals from xylose using native and engineered yeast strains},
author = {Kwak, Suryang and Jo, Jung Hyun and Yun, Eun Ju and Jin, Yong-Su and Seo, Jin-Ho},
abstractNote = {Numerous metabolic engineering strategies have allowed yeasts to efficiently assimilate xylose, the second most abundant sugar component of lignocellulosic biomass. During the investigation of xylose utilization by yeasts, a global rewiring of metabolic networks upon xylose cultivation has been captured, as opposed to a pattern of glucose repression. A clear understanding of the xylose-induced metabolic reprogramming in yeast would shed light on the optimization of yeast-based bioprocesses to produce biofuels and chemicals using xylose. In this review, we delved into characteristics of yeast xylose metabolism, and potential benefits of using xylose as a carbon source to produce various biochemicals with examples. Transcriptomic and metabolomic patterns of xylose-grown yeast cells were distinct from those on glucose—a conventional sugar of industrial biotechnology—and the gap might lead to opportunities to produce biochemicals efficiently. Indeed, limited glycolytic metabolic fluxes during xylose utilization could result in enhanced production of metabolites whose biosynthetic pathways compete for precursors with ethanol fermentation. Also, alleviation of glucose repression on cytosolic acetyl coenzyme A (acetyl-CoA) synthesis, and respiratory energy metabolism during xylose utilization enhanced production of acetyl-CoA derivatives. Consideration of singular properties of xylose metabolism, such as redox cofactor imbalance between xylose reductase and xylitol dehydrogenase, is necessary to maximize these positive xylose effects. As a result, this review argues the importance and benefits of xylose utilization as not only a way of expanding a substrate range, but also an effective environmental perturbation for the efficient production of advanced biofuels and chemicals in yeasts.},
doi = {10.1016/j.biotechadv.2018.12.003},
journal = {Biotechnology Advances},
number = 2,
volume = 37,
place = {United States},
year = {Fri Mar 01 00:00:00 EST 2019},
month = {Fri Mar 01 00:00:00 EST 2019}
}
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Metabolomic and 13 C-metabolic flux analysis of a xylose-consuming Saccharomyces cerevisiae strain expressing xylose isomerase: Xylose Metabolic Flux Analysis
journal, November 2014

  • Wasylenko, Thomas M.; Stephanopoulos, Gregory
  • Biotechnology and Bioengineering, Vol. 112, Issue 3
  • DOI: 10.1002/bit.25447

Recycling Carbon Dioxide during Xylose Fermentation by Engineered Saccharomyces cerevisiae
journal, October 2016

Optimizing pentose utilization in yeast: the need for novel tools and approaches
journal, January 2010

  • Young, Eric; Lee, Sun-Mi; Alper, Hal
  • Biotechnology for Biofuels, Vol. 3, Issue 1
  • DOI: 10.1186/1754-6834-3-24

A molecular transporter engineering approach to improving xylose catabolism in Saccharomyces cerevisiae
journal, July 2012

RNA-Seq of the xylose-fermenting yeast Scheffersomyces stipitis cultivated in glucose or xylose
journal, November 2011

  • Yuan, Tiezheng; Ren, Yan; Meng, Kun
  • Applied Microbiology and Biotechnology, Vol. 92, Issue 6
  • DOI: 10.1007/s00253-011-3607-6

Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion
journal, December 2015

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    Works referencing / citing this record:

    Producing Biochemicals in Yarrowia lipolytica from Xylose through a Strain Mating Approach
    journal, September 2019

    Redirection of the Glycolytic Flux Enhances Isoprenoid Production in Saccharomyces cerevisiae
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    • Kwak, Suryang; Yun, Eun Ju; Lane, Stephan
    • Biotechnology Journal, Vol. 15, Issue 2
    • DOI: 10.1002/biot.201900173

    Simulating Extracellular Glucose Signals Enhances Xylose Metabolism in Recombinant Saccharomyces cerevisiae
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    Rewired cellular signaling coordinates sugar and hypoxic responses for anaerobic xylose fermentation in yeast
    journal, March 2019

    A Thi2p Regulatory Network Controls the Post-glucose Effect of Xylose Utilization in Saccharomyces cerevisiae
    journal, July 2019

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