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Title: Characterization of a large sex determination region in Salix purpurea L. (Salicaceae)

Abstract

Dioecy has evolved numerous times in plants, but heteromorphic sex chromosomes are apparently rare. Sex determination has been studied in multiple Salix and Populus (Salicaceae) species, and P. trichocarpa has an XY sex determination system on chromosome 19, while S. suchowensis and S. viminalis have a ZW system on chromosome 15. Here in this paper, we use whole genome sequencing coupled with quantitative trait locus mapping and a genome-wide association study to characterize the genomic composition of the non-recombining portion of the sex determination region. We demonstrate that Salix purpurea also has a ZW system on chromosome 15. The sex determination region has reduced recombination, high structural polymorphism, an abundance of transposable elements, and contains genes that are involved in sex expression in other plants. We also show that chromosome 19 contains sex-associated markers in this S. purpurea assembly, along with other autosomes. This raises the intriguing possibility of a translocation of the sex determination region within the Salicaceae lineage, suggesting a common evolutionary origin of the Populus and Salix sex determination loci.

Authors:
 [1];  [1];  [1];  [2];  [2];  [1];  [3];  [4];  [5]; ORCiD logo [6];  [2]; ORCiD logo [1]
  1. West Virginia Univ., Morgantown, WV (United States). Dept. of Biology
  2. Cornell Univ., Geneva, NY (United States). School of Integrative Plant Science, New York State Agricultural Experiment Station, Horticulture Section
  3. HudsonAlpha Inst. of Biotechnology, Huntsville, AL (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  4. HudsonAlpha Inst. of Biotechnology, Huntsville, AL (United States)
  5. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  6. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1494877
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Molecular Genetics and Genomics
Additional Journal Information:
Journal Volume: 293; Journal Issue: 6; Journal ID: ISSN 1617-4615
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Sex; Salix; Genome; Suppressed recombination; Dioecy

Citation Formats

Zhou, Ran, Macaya-Sanz, David, Rodgers-Melnick, Eli, Carlson, Craig H., Gouker, Fred E., Evans, Luke M., Schmutz, Jeremy, Jenkins, Jerry W., Yan, Juying, Tuskan, Gerald A., Smart, Lawrence B., and DiFazio, Stephen P. Characterization of a large sex determination region in Salix purpurea L. (Salicaceae). United States: N. p., 2018. Web. doi:10.1007/s00438-018-1473-y.
Zhou, Ran, Macaya-Sanz, David, Rodgers-Melnick, Eli, Carlson, Craig H., Gouker, Fred E., Evans, Luke M., Schmutz, Jeremy, Jenkins, Jerry W., Yan, Juying, Tuskan, Gerald A., Smart, Lawrence B., & DiFazio, Stephen P. Characterization of a large sex determination region in Salix purpurea L. (Salicaceae). United States. doi:10.1007/s00438-018-1473-y.
Zhou, Ran, Macaya-Sanz, David, Rodgers-Melnick, Eli, Carlson, Craig H., Gouker, Fred E., Evans, Luke M., Schmutz, Jeremy, Jenkins, Jerry W., Yan, Juying, Tuskan, Gerald A., Smart, Lawrence B., and DiFazio, Stephen P. Wed . "Characterization of a large sex determination region in Salix purpurea L. (Salicaceae)". United States. doi:10.1007/s00438-018-1473-y. https://www.osti.gov/servlets/purl/1494877.
@article{osti_1494877,
title = {Characterization of a large sex determination region in Salix purpurea L. (Salicaceae)},
author = {Zhou, Ran and Macaya-Sanz, David and Rodgers-Melnick, Eli and Carlson, Craig H. and Gouker, Fred E. and Evans, Luke M. and Schmutz, Jeremy and Jenkins, Jerry W. and Yan, Juying and Tuskan, Gerald A. and Smart, Lawrence B. and DiFazio, Stephen P.},
abstractNote = {Dioecy has evolved numerous times in plants, but heteromorphic sex chromosomes are apparently rare. Sex determination has been studied in multiple Salix and Populus (Salicaceae) species, and P. trichocarpa has an XY sex determination system on chromosome 19, while S. suchowensis and S. viminalis have a ZW system on chromosome 15. Here in this paper, we use whole genome sequencing coupled with quantitative trait locus mapping and a genome-wide association study to characterize the genomic composition of the non-recombining portion of the sex determination region. We demonstrate that Salix purpurea also has a ZW system on chromosome 15. The sex determination region has reduced recombination, high structural polymorphism, an abundance of transposable elements, and contains genes that are involved in sex expression in other plants. We also show that chromosome 19 contains sex-associated markers in this S. purpurea assembly, along with other autosomes. This raises the intriguing possibility of a translocation of the sex determination region within the Salicaceae lineage, suggesting a common evolutionary origin of the Populus and Salix sex determination loci.},
doi = {10.1007/s00438-018-1473-y},
journal = {Molecular Genetics and Genomics},
number = 6,
volume = 293,
place = {United States},
year = {2018},
month = {7}
}

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Cited by: 11 works
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    Works referencing / citing this record:

    Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population
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