Enhancing microRNA 167A expression in seed decreases the α-linolenic acid content and increases seed size in Camelina sativa
Abstract
Despite well established roles of microRNAs in plant development, few aspects have been addressed to understand their effects in seeds especially on lipid metabolism. In this study, we showed that overexpressing microRNA167A (miR167OE) in camelina (Camelina sativa) under a seed-specific promoter changed fatty acid composition and increased seed size. Specifically, the miR167OE seeds had a lower a-linolenic acid with a concomitantly higher linoleic acid content than the wild-type. This decreased level of fatty acid desatura- tion corresponded to a decreased transcriptional expression of the camelina fatty acid desaturase3 (CsFAD3) in developing seeds. MiR167 targeted the transcription factor auxin response factor (CsARF8) in camelina, as had been reported previously in Arabidopsis. Chromatin immunoprecipitation experiments combined with transcriptome analysis indicated that CsARF8 bound to promoters of camelina bZIP67 and ABI3 genes. These transcription factors directly or through the ABI3-bZIP12 pathway regulate CsFAD3 expression and affect a-linolenic acid accumulation. In addition, to decipher the miR167A-CsARF8 mediated transcriptional cascade for CsFAD3 suppression, transcriptome analysis was conducted to implicate mechanisms that regu- late seed size in camelina. Expression levels of many genes were altered in miR167OE, including orthologs that have previously been identified to affect seed size in other plants. Most notably, genes for seedmore »
- Authors:
-
- Montana State Univ., Bozeman, MT (United States)
- Montana State Univ., Bozeman, MT (United States); Shanxi Agricultural Univ., Taigu (China)
- HudsonAlpha Inst. of Biotechnology, Huntsville, AL (United States)
- HudsonAlpha Inst. of Biotechnology, Huntsville, AL (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Montana State Univ., Bozeman, MT (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); USDA
- OSTI Identifier:
- 1616082
- Alternate Identifier(s):
- OSTI ID: 1494844; OSTI ID: 1647217
- Report Number(s):
- 1
Journal ID: ISSN 0960-7412; ark:/13030/qt27x8k3xz
- Grant/Contract Number:
- AC02-05CH11231; SC00164900; DBI-1339358; SC0016400; DBI‐1339358; AC02‐05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Volume: 98; Journal Issue: 2; Journal ID: ISSN 0960-7412
- Publisher:
- Society for Experimental Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; microRNA 167; transcription factors; Camelina sativa; fatty acid desaturase 3; a-linolenic acid; seed size
Citation Formats
Na, GunNam, Mu, Xiaopeng, Grabowski, Paul, Schmutz, Jeremy, and Lu, Chaofu. Enhancing microRNA 167A expression in seed decreases the α-linolenic acid content and increases seed size in Camelina sativa. United States: N. p., 2019.
Web. doi:10.1111/tpj.14223.
Na, GunNam, Mu, Xiaopeng, Grabowski, Paul, Schmutz, Jeremy, & Lu, Chaofu. Enhancing microRNA 167A expression in seed decreases the α-linolenic acid content and increases seed size in Camelina sativa. United States. https://doi.org/10.1111/tpj.14223
Na, GunNam, Mu, Xiaopeng, Grabowski, Paul, Schmutz, Jeremy, and Lu, Chaofu. Thu .
"Enhancing microRNA 167A expression in seed decreases the α-linolenic acid content and increases seed size in Camelina sativa". United States. https://doi.org/10.1111/tpj.14223. https://www.osti.gov/servlets/purl/1616082.
@article{osti_1616082,
title = {Enhancing microRNA 167A expression in seed decreases the α-linolenic acid content and increases seed size in Camelina sativa},
author = {Na, GunNam and Mu, Xiaopeng and Grabowski, Paul and Schmutz, Jeremy and Lu, Chaofu},
abstractNote = {Despite well established roles of microRNAs in plant development, few aspects have been addressed to understand their effects in seeds especially on lipid metabolism. In this study, we showed that overexpressing microRNA167A (miR167OE) in camelina (Camelina sativa) under a seed-specific promoter changed fatty acid composition and increased seed size. Specifically, the miR167OE seeds had a lower a-linolenic acid with a concomitantly higher linoleic acid content than the wild-type. This decreased level of fatty acid desatura- tion corresponded to a decreased transcriptional expression of the camelina fatty acid desaturase3 (CsFAD3) in developing seeds. MiR167 targeted the transcription factor auxin response factor (CsARF8) in camelina, as had been reported previously in Arabidopsis. Chromatin immunoprecipitation experiments combined with transcriptome analysis indicated that CsARF8 bound to promoters of camelina bZIP67 and ABI3 genes. These transcription factors directly or through the ABI3-bZIP12 pathway regulate CsFAD3 expression and affect a-linolenic acid accumulation. In addition, to decipher the miR167A-CsARF8 mediated transcriptional cascade for CsFAD3 suppression, transcriptome analysis was conducted to implicate mechanisms that regu- late seed size in camelina. Expression levels of many genes were altered in miR167OE, including orthologs that have previously been identified to affect seed size in other plants. Most notably, genes for seed coat development such as suberin and lignin biosynthesis were down-regulated. This study provides valuable insights into the regulatory mechanism of fatty acid metabolism and seed size determination, and suggests possible approaches to improve these important traits in camelina.},
doi = {10.1111/tpj.14223},
journal = {The Plant Journal},
number = 2,
volume = 98,
place = {United States},
year = {Thu Jan 03 00:00:00 EST 2019},
month = {Thu Jan 03 00:00:00 EST 2019}
}
Web of Science
Figures / Tables:
Works referenced in this record:
Gene regulation during late embryogenesis: the RY motif of maturation-specific gene promoters is a direct target of the FUS3 gene product
journal, March 2000
- Reidt, Wim; Wohlfarth, Thomas; Ellerstrom, Mats
- The Plant Journal, Vol. 21, Issue 5
Seed-Development Programs: A Systems Biology–Based Comparison Between Dicots and Monocots
journal, April 2013
- Sreenivasulu, Nese; Wobus, Ulrich
- Annual Review of Plant Biology, Vol. 64, Issue 1
Lipid biochemists salute the genome
journal, March 2010
- Wallis, James G.; Browse, John
- The Plant Journal, Vol. 61, Issue 6
Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs
journal, January 2007
- Varkonyi-Gasic, Erika; Wu, Rongmei; Wood, Marion
- Plant Methods, Vol. 3, Issue 1
Selective gene dosage by CRISPR-Cas9 genome editing in hexaploid Camelina sativa
journal, April 2017
- Morineau, Céline; Bellec, Yannick; Tellier, Frédérique
- Plant Biotechnology Journal, Vol. 15, Issue 6
Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis
journal, May 2008
- Santos-Mendoza, Monica; Dubreucq, Bertrand; Baud, Sébastien
- The Plant Journal, Vol. 54, Issue 4
Identification of acyltransferases required for cutin biosynthesis and production of cutin with suberin-like monomers
journal, November 2007
- Li, Y.; Beisson, F.; Koo, A. J. K.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 46
The transcription factor MYB115 contributes to the regulation of proanthocyanidin biosynthesis and enhances fungal resistance in poplar
journal, April 2017
- Wang, Lijun; Ran, Lingyu; Hou, Yisu
- New Phytologist, Vol. 215, Issue 1
Regulation and evolution of the interaction of the seed B3 transcription factors with NF-Y subunits
journal, October 2017
- Boulard, C.; Fatihi, A.; Lepiniec, L.
- Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Vol. 1860, Issue 10
Two high linolenic mutants of Arabidopsis thaliana contain megabase-scale genome duplications encompassing the FAD3 locus: High linolenic acid mutants of A. thaliana
journal, October 2011
- O’Neill, Carmel M.; Baker, David; Bennett, Gawain
- The Plant Journal, Vol. 68, Issue 5
Mutagenesis of the FAE1 genes significantly changes fatty acid composition in seeds of Camelina sativa
journal, February 2018
- Ozseyhan, Mehmet E.; Kang, Jinling; Mu, Xiaopeng
- Plant Physiology and Biochemistry, Vol. 123
Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter
journal, May 2007
- Vigeolas, Helene; Waldeck, Peter; Zank, Thorsten
- Plant Biotechnology Journal, Vol. 5, Issue 3
Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis
journal, November 1992
- Arondel, V.; Lemieux, B.; Hwang, I.
- Science, Vol. 258, Issue 5086
The Acyltransferase GPAT5 Is Required for the Synthesis of Suberin in Seed Coat and Root of Arabidopsis
journal, January 2007
- Beisson, Fred; Li, Yonghua; Bonaventure, Gustavo
- The Plant Cell, Vol. 19, Issue 1
Two Acyltransferases Contribute Differently to Linolenic Acid Levels in Seed Oil
journal, February 2017
- Marmon, Sofia; Sturtevant, Drew; Herrfurth, Cornelia
- Plant Physiology, Vol. 173, Issue 4
Networks controlling seed size in Arabidopsis
journal, February 2015
- Orozco-Arroyo, Gregorio; Paolo, Dario; Ezquer, Ignacio
- Plant Reproduction, Vol. 28, Issue 1
“Designer” Biodiesel: Optimizing Fatty Ester Composition to Improve Fuel Properties †
journal, March 2008
- Knothe, Gerhard
- Energy & Fuels, Vol. 22, Issue 2
Biodiesel from Camelina sativa: A comprehensive characterisation
journal, March 2013
- Ciubota-Rosie, Camelia; Ruiz, José Ramón; Ramos, María Jesús
- Fuel, Vol. 105
Hormonal regulation of tissue-specific ectopic expression of an Arabidopsis endoplasmic reticulum-type ω-3 fatty acid desaturase (FAD3) gene
journal, October 2001
- Matsuda, Osamu; Watanabe, Chizuru; Iba, Koh
- Planta, Vol. 213, Issue 6
WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis
journal, September 2004
- Cernac, Alex; Benning, Christoph
- The Plant Journal, Vol. 40, Issue 4, p. 575-585
The Protein Kinase SnRK2.6 Mediates the Regulation of Sucrose Metabolism and Plant Growth in Arabidopsis
journal, March 2010
- Zheng, Zhifu; Xu, Xiaoping; Crosley, Rodney A.
- Plant Physiology, Vol. 153, Issue 1
Two Arabidopsis 3-ketoacyl CoA synthase genes, KCS20 and KCS2 / DAISY , are functionally redundant in cuticular wax and root suberin biosynthesis, but differentially controlled by osmotic stress
journal, November 2009
- Lee, Saet-Buyl; Jung, Su-Jin; Go, Young-Sam
- The Plant Journal, Vol. 60, Issue 3
An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in Arabidopsis plants
journal, May 2008
- Saleh, Abdelaty; Alvarez-Venegas, Raúl; Avramova, Zoya
- Nature Protocols, Vol. 3, Issue 6
Identification of Direct Targets of FUSCA3, a Key Regulator of Arabidopsis Seed Development
journal, January 2013
- Wang, Fangfang; Perry, Sharyn E.
- Plant Physiology, Vol. 161, Issue 3
Fast and sensitive protein alignment using DIAMOND
journal, November 2014
- Buchfink, Benjamin; Xie, Chao; Huson, Daniel H.
- Nature Methods, Vol. 12, Issue 1
FUSCA3 activates triacylglycerol accumulation in Arabidopsis seedlings and tobacco BY2 cells
journal, August 2016
- Zhang, Meng; Cao, Xia; Jia, Qingli
- The Plant Journal, Vol. 88, Issue 1
Genes directly regulated by LEAFY COTYLEDON2 provide insight into the control of embryo maturation and somatic embryogenesis
journal, February 2006
- Braybrook, S. A.; Stone, S. L.; Park, S.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 9
Acyl-Lipid Metabolism
journal, January 2013
- Li-Beisson, Yonghua; Shorrosh, Basil; Beisson, Fred
- The Arabidopsis Book, Vol. 11
The plasma membrane–bound phospholipase Dδ enhances freezing tolerance in Arabidopsis thaliana
journal, March 2004
- Li, Weiqi; Li, Maoyin; Zhang, Wenhua
- Nature Biotechnology, Vol. 22, Issue 4
Arabidopsis LEAFY COTYLEDON2 induces maturation traits and auxin activity: Implications for somatic embryogenesis
journal, February 2008
- Stone, S. L.; Braybrook, S. A.; Paula, S. L.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 8
Integration of epigenetic and genetic controls of seed size by cytokinin in Arabidopsis
journal, September 2013
- Li, J.; Nie, X.; Tan, J. L. H.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 38
Characterization of the morphological changes and fatty acid profile of developing Camelina sativa seeds
journal, October 2013
- Rodríguez-Rodríguez, Manuel Fernando; Sánchez-García, Alicia; Salas, Joaquín J.
- Industrial Crops and Products, Vol. 50
Identification of three genes encoding microsomal oleate desaturases (FAD2) from the oilseed crop Camelina sativa
journal, February 2011
- Kang, Jinling; Snapp, Anna R.; Lu, Chaofu
- Plant Physiology and Biochemistry, Vol. 49, Issue 2
ABI3 and PIL5 Collaboratively Activate the Expression of SOMNUS by Directly Binding to Its Promoter in Imbibed Arabidopsis Seeds
journal, April 2011
- Park, Jeongmoo; Lee, Nayoung; Kim, Woohyun
- The Plant Cell, Vol. 23, Issue 4
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis
journal, June 2007
- Yoo, Sang-Dong; Cho, Young-Hee; Sheen, Jen
- Nature Protocols, Vol. 2, Issue 7
WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis: Oil synthesis regulatory network in seeds
journal, April 2007
- Baud, Sébastien; Mendoza, Monica Santos; To, Alexandra
- The Plant Journal, Vol. 50, Issue 5
Camelina-derived jet fuel and diesel: Sustainable advanced biofuels
journal, June 2010
- Shonnard, David R.; Williams, Larry; Kalnes, Tom N.
- Environmental Progress & Sustainable Energy, Vol. 29, Issue 3
Auxin-regulated changes of fatty acid content and composition in soybean zygotic embryo cotyledons
journal, March 1995
- Liu, Wennuan; Hildebrand, David F.; Collins, Glenn B.
- Plant Science, Vol. 106, Issue 1
Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas
journal, January 2017
- Sun, Yanwei; Wang, Chunming; Wang, Ning
- Scientific Reports, Vol. 7, Issue 1
Camelina seed transcriptome: a tool for meal and oil improvement and translational research
journal, April 2013
- Nguyen, Huu T.; Silva, Jillian E.; Podicheti, Ram
- Plant Biotechnology Journal, Vol. 11, Issue 6
Acyl Editing and Headgroup Exchange Are the Major Mechanisms That Direct Polyunsaturated Fatty Acid Flux into Triacylglycerols
journal, August 2012
- Bates, Philip D.; Fatihi, Abdelhak; Snapp, Anna R.
- Plant Physiology, Vol. 160, Issue 3
microRNA regulation of fruit growth
journal, March 2015
- José Ripoll, Juan; Bailey, Lindsay J.; Mai, Quynh-Anh
- Nature Plants, Vol. 1, Issue 4
Auxin controls seed dormancy through stimulation of abscisic acid signaling by inducing ARF-mediated ABI3 activation in Arabidopsis
journal, August 2013
- Liu, X.; Zhang, H.; Zhao, Y.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 38
MYB118 Represses Endosperm Maturation in Seeds of Arabidopsis
journal, September 2014
- Barthole, G.; To, A.; Marchive, C.
- The Plant Cell, Vol. 26, Issue 9
Dual DNA Binding Property of ABA insensitive 3 Like Factors Targeted to Promoters Responsive to ABA and Auxin
journal, November 2005
- Nag, Ronita; Maity, Manas Kanti; DasGupta, Maitrayee
- Plant Molecular Biology, Vol. 59, Issue 5
bZIP67 Regulates the Omega-3 Fatty Acid Content of Arabidopsis Seed Oil by Activating FATTY ACID DESATURASE3
journal, August 2013
- Mendes, Ana; Kelly, Amélie A.; van Erp, Harrie
- The Plant Cell, Vol. 25, Issue 8
Increasing seed mass and oil content in transgenic Arabidopsis by the overexpression of wri1-like gene from Brassica napus
journal, January 2010
- Liu, Jing; Hua, Wei; Zhan, Gaomiao
- Plant Physiology and Biochemistry, Vol. 48, Issue 1
Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction
journal, October 2006
- Wu, M. -F.; Tian, Q.; Reed, J. W.
- Development, Vol. 133, Issue 21
Leafy cotyledon genes are essential for induction of somatic embryogenesis of Arabidopsis
journal, July 2005
- Gaj, Malgorzata D.; Zhang, Shibo; Harada, John J.
- Planta, Vol. 222, Issue 6
Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling: TFs of A. thaliana R2R3-MYB subgroup 7
journal, April 2007
- Stracke, Ralf; Ishihara, Hirofumi; Huep, Gunnar
- The Plant Journal, Vol. 50, Issue 4
MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis
journal, December 2010
- Nodine, M. D.; Bartel, D. P.
- Genes & Development, Vol. 24, Issue 23
The Nonspecific Lipid Transfer Protein AtLtpI-4 Is Involved in Suberin Formation of Arabidopsis thaliana Crown Galls
journal, September 2016
- Deeken, Rosalia; Saupe, Stefanie; Klinkenberg, Joern
- Plant Physiology, Vol. 172, Issue 3
Signaling pathways of seed size control in plants
journal, October 2016
- Li, Na; Li, Yunhai
- Current Opinion in Plant Biology, Vol. 33
The Transcription Factor FUSCA3 Controls Developmental Timing in Arabidopsis through the Hormones Gibberellin and Abscisic Acid
journal, September 2004
- Gazzarrini, Sonia; Tsuchiya, Yuichiro; Lumba, Shelley
- Developmental Cell, Vol. 7, Issue 3
TTG1 complex MYBs, MYB5 and TT2, control outer seed coat differentiation
journal, January 2009
- Gonzalez, Antonio; Mendenhall, John; Huo, Yujia
- Developmental Biology, Vol. 325, Issue 2
Natural allelic variation at seed size loci in relation to other life history traits of Arabidopsis thaliana
journal, April 1999
- Alonso-Blanco, C.; Blankestijn-de Vries, H.; Hanhart, C. J.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 8
Flavonoids and the regulation of seed size in Arabidopsis
journal, March 2014
- Doughty, James; Aljabri, Maha; Scott, Rod J.
- Biochemical Society Transactions, Vol. 42, Issue 2
New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications
journal, April 2011
- Lu, Chaofu; Napier, Johnathan A.; Clemente, Thomas E.
- Current Opinion in Biotechnology, Vol. 22, Issue 2
Histone acetyltransferase general control non-repressed protein 5 (GCN5) affects the fatty acid composition of Arabidopsis thaliana seeds by acetylating fatty acid desaturase3 ( FAD3 )
journal, September 2016
- Wang, Tianya; Xing, Jiewen; Liu, Xinye
- The Plant Journal, Vol. 88, Issue 5
Activation and repression of transcription by auxin-response factors
journal, May 1999
- Ulmasov, T.; Hagen, G.; Guilfoyle, T. J.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 10
Three Arabidopsis Fatty Acyl-Coenzyme A Reductases, FAR1, FAR4, and FAR5, Generate Primary Fatty Alcohols Associated with Suberin Deposition
journal, June 2010
- Domergue, Frédéric; Vishwanath, Sollapura J.; Joubès, Jérôme
- Plant Physiology, Vol. 153, Issue 4
Leafy Cotyledon Mutants of Arabidopsis
journal, August 1994
- Meinke, David W.; Franzmann, Linda H.; Nickle, Todd C.
- The Plant Cell, Vol. 6, Issue 8
A transgene design for enhancing oil content in Arabidopsis and Camelina seeds
journal, February 2018
- Zhu, Yerong; Xie, Linan; Chen, Grace Q.
- Biotechnology for Biofuels, Vol. 11, Issue 1
Natural variation in ARF18 gene simultaneously affects seed weight and silique length in polyploid rapeseed
journal, August 2015
- Liu, Jing; Hua, Wei; Hu, Zhiyong
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 37
The turnip Mutant of Arabidopsis Reveals That LEAFY COTYLEDON1 Expression Mediates the Effects of Auxin and Sugars to Promote Embryonic Cell Identity
journal, August 2006
- Casson, Stuart A.; Lindsey, Keith
- Plant Physiology, Vol. 142, Issue 2
Complexity and robustness of the flavonoid transcriptional regulatory network revealed by comprehensive analyses of MYB-bHLH-WDR complexes and their targets in Arabidopsis seed
journal, December 2013
- Xu, Wenjia; Grain, Damaris; Bobet, Sophie
- New Phytologist, Vol. 202, Issue 1
Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching
journal, July 2013
- Zhang, Yu-Chan; Yu, Yang; Wang, Cong-Ying
- Nature Biotechnology, Vol. 31, Issue 9
Brassinosteroid Regulates Seed Size and Shape in Arabidopsis
journal, June 2013
- Jiang, Wen-Bo; Huang, Hui-Ya; Hu, Yu-Wei
- Plant Physiology, Vol. 162, Issue 4
Expression of ZmLEC1 and ZmWRI1 Increases Seed Oil Production in Maize
journal, May 2010
- Shen, Bo; Allen, William B.; Zheng, Peizhong
- Plant Physiology, Vol. 153, Issue 3
The Acyl-CoA Synthetase Encoded by LACS2 Is Essential for Normal Cuticle Development in Arabidopsis
journal, February 2004
- Schnurr, Judy; Shockey, Jay; Browse, John
- The Plant Cell, Vol. 16, Issue 3
Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds
journal, October 2015
- Kanai, Masatake; Mano, Shoji; Kondo, Maki
- Plant Biotechnology Journal, Vol. 14, Issue 5
Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato
journal, April 2014
- Liu, Ning; Wu, Shan; Van Houten, Jason
- Journal of Experimental Botany, Vol. 65, Issue 9
CYP86B1 Is Required for Very Long Chain ω -Hydroxyacid and α , ω -Dicarboxylic Acid Synthesis in Root and Seed Suberin Polyester
journal, June 2009
- Compagnon, Vincent; Diehl, Patrik; Benveniste, Irène
- Plant Physiology, Vol. 150, Issue 4
MicroRNAs Regulate the Timing of Embryo Maturation in Arabidopsis
journal, February 2011
- Willmann, Matthew R.; Mehalick, Andrew J.; Packer, Rachel L.
- Plant Physiology, Vol. 155, Issue 4
Seed Development and Differentiation: A Role for Metabolic Regulation
journal, July 2004
- Borisjuk, L.; Rolletschek, H.; Radchuk, R.
- Plant Biology, Vol. 6, Issue 4
The Arabidopsis Information Resource (TAIR): improved gene annotation and new tools
journal, December 2011
- Lamesch, Philippe; Berardini, Tanya Z.; Li, Donghui
- Nucleic Acids Research, Vol. 40, Issue D1
The FUS3 transcription factor functions through the epidermal regulator TTG1 during embryogenesis in Arabidopsis
journal, January 2004
- Tsuchiya, Yuichiro; Nambara, Eiji; Naito, Satoshi
- The Plant Journal, Vol. 37, Issue 1
MYB 56 Encoding a R2R3 MYB Transcription Factor Regulates Seed Size in Arabidopsis thaliana : MYB56 and Seed Size
journal, September 2013
- Zhang, Yanjie; Liang, Wanqi; Shi, Jianxin
- Journal of Integrative Plant Biology, Vol. 55, Issue 11
A mutant of Arabidopsis which is defective in seed development and storage protein accumulation is a new abi3 allele
journal, July 1992
- Nambara, Eiji; Naito, Satoshi; McCourt, Peter
- The Plant Journal, Vol. 2, Issue 4
Three Classes of Abscisic Acid (ABA)-Insensitive Mutations of Arabidopsis Define Genes that Control Overlapping Subsets of ABA Responses
journal, November 1990
- Finkelstein, Ruth R.; Somerville, Chris R.
- Plant Physiology, Vol. 94, Issue 3
edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
journal, November 2009
- Robinson, M. D.; McCarthy, D. J.; Smyth, G. K.
- Bioinformatics, Vol. 26, Issue 1
The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure
journal, April 2014
- Kagale, Sateesh; Koh, Chushin; Nixon, John
- Nature Communications, Vol. 5, Issue 1
A Regulatory Network for Coordinated Flower Maturation
journal, February 2012
- Reeves, Paul H.; Ellis, Christine M.; Ploense, Sara E.
- PLoS Genetics, Vol. 8, Issue 2
A New Dynamin-Like Protein, ADL6, Is Involved in Trafficking from the trans -Golgi Network to the Central Vacuole in Arabidopsis
journal, July 2001
- Jin, Jing Bo; Kim, Young A.; Kim, Soo Jin
- The Plant Cell, Vol. 13, Issue 7
Epigenetic mechanisms governing seed development in plants
journal, December 2006
- Köhler, Claudia; Makarevich, Grigory
- EMBO reports, Vol. 7, Issue 12
The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs
journal, December 2005
- Schruff, M. C.
- Development, Vol. 133, Issue 2
Identification of MicroRNAs and Transcript Targets in Camelina sativa by Deep Sequencing and Computational Methods
journal, March 2015
- Poudel, Saroj; Aryal, Niranjan; Lu, Chaofu
- PLOS ONE, Vol. 10, Issue 3
Module-specific regulation of the β-phaseolin promoter during embryogenesis
journal, February 2003
- Chandrasekharan, Mahesh B.; Bishop, Kenneth J.; Hall, Timothy C.
- The Plant Journal, Vol. 33, Issue 5
Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing
journal, January 2017
- Jiang, Wen Zhi; Henry, Isabelle M.; Lynagh, Peter G.
- Plant Biotechnology Journal, Vol. 15, Issue 5
AUXIN RESPONSE FACTOR8 Is a Negative Regulator of Fruit Initiation in Arabidopsis
journal, July 2006
- Goetz, Marc; Vivian-Smith, Adam; Johnson, Susan D.
- The Plant Cell, Vol. 18, Issue 8
Abscisic acid or high osmoticum promote accumulation of long-chain fatty acids in developing embryos of Brassica napus
journal, January 1989
- Finkelstein, Ruth; Somerville, Chris
- Plant Science, Vol. 61, Issue 2
MYB107 and MYB9 Homologs Regulate Suberin Deposition in Angiosperms
journal, September 2016
- Lashbrooke, Justin; Cohen, Hagai; Levy-Samocha, Dorit
- The Plant Cell, Vol. 28, Issue 9
A high-throughput screen for genes from castor that boost hydroxy fatty acid accumulation in seed oils of transgenic Arabidopsis
journal, March 2006
- Lu, Chaofu; Fulda, Martin; Wallis, James G.
- The Plant Journal, Vol. 45, Issue 5
Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation
journal, September 2007
- Lu, Chaofu; Kang, Jinling
- Plant Cell Reports, Vol. 27, Issue 2
A fatty acid condensing enzyme from Physaria fendleri increases hydroxy fatty acid accumulation in transgenic oilseeds of Camelina sativa
journal, July 2014
- Snapp, Anna R.; Kang, Jinling; Qi, Xiaoli
- Planta, Vol. 240, Issue 3
Deposition and localization of lipid polyester in developing seeds of Brassica napus and Arabidopsis thaliana: Lipid polyesters in seed coats
journal, October 2007
- Molina, Isabel; Ohlrogge, John B.; Pollard, Mike
- The Plant Journal, Vol. 53, Issue 3
Overexpression of the FAD3 Desaturase Gene in a Mutant of Arabidopsis
journal, August 1997
- Shah, S.; Xin, Z.; Browse, J.
- Plant Physiology, Vol. 114, Issue 4
Physiological and developmental regulation of seed oil production
journal, July 2010
- Baud, Sébastien; Lepiniec, Loïc
- Progress in Lipid Research, Vol. 49, Issue 3
An enzyme regulating triacylglycerol composition is encoded by the ROD1 gene of Arabidopsis
journal, October 2009
- Lu, C.; Xin, Z.; Ren, Z.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 44
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
journal, December 2014
- Love, Michael I.; Huber, Wolfgang; Anders, Simon
- Genome Biology, Vol. 15, Issue 12
MYB58 and MYB63 Are Transcriptional Activators of the Lignin Biosynthetic Pathway during Secondary Cell Wall Formation in Arabidopsis
journal, January 2009
- Zhou, Jianli; Lee, Chanhui; Zhong, Ruiqin
- The Plant Cell, Vol. 21, Issue 1
Identification and molecular characterization of miRNAs and their target genes associated with seed development through small RNA sequencing in chickpea
journal, February 2021
- Pradhan, Seema; Verma, Subodh; Chakraborty, Anirban
- Functional & Integrative Genomics, Vol. 21, Issue 2
Leafy Cotyledon Mutants of Arabidopsis.
journal, August 1994
- Meinke, D. W.; Franzmann, L. H.; Nickle, T. C.
- The Plant Cell
Datura genome reveals duplications of psychoactive alkaloid biosynthetic genes and high mutation rate following tissue culture
journal, March 2021
- Rajewski, Alex; Carter-House, Derreck; Stajich, Jason
- BMC Genomics, Vol. 22, Issue 1
Polinton-like viruses are abundant in aquatic ecosystems
journal, January 2021
- Bellas, Christopher M.; Sommaruga, Ruben
- Microbiome, Vol. 9, Issue 1
Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato
text, January 2014
- Zhangjun, Fei,; Ning, Liu,; Biao, Ding,
- The University of North Carolina at Chapel Hill University Libraries
A Regulatory Network for Coordinated Flower Maturation
text, January 2012
- E., Farmer, Edward; Ina, Haupt,; M., Ellis, Christine
- The University of North Carolina at Chapel Hill University Libraries
The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
book, January 2009
- Walker, John M.
- Springer Protocols Handbooks
A New Dynamin-Like Protein, ADL6, Is Involved in Trafficking from the trans-Golgi Network to the Central Vacuole in Arabidopsis
journal, July 2001
- Jin, Jing Bo; Kim, Young A.; Kim, Soo Jin
- The Plant Cell, Vol. 13, Issue 7
Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction
text, January 2006
- Feng, Wu, Miin; Qing, Tian,; W., Reed, Jason
- The University of North Carolina at Chapel Hill University Libraries
Works referencing / citing this record:
Mapping quantitative trait loci for seed traits in Camelina sativa
journal, June 2019
- King, Kevin; Li, Huang; Kang, Jinling
- Theoretical and Applied Genetics, Vol. 132, Issue 9
Into the Seed: Auxin Controls Seed Development and Grain Yield
journal, February 2020
- Cao, Jinshan; Li, Guoji; Qu, Dejie
- International Journal of Molecular Sciences, Vol. 21, Issue 5
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